FastQCFastQC Report
Wed 30 Apr 2025
1D5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20050766
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5526152.7560792440548156Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC2435991.2149111909240773No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1684270.8400028208398622No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1544870.7704792924120705No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1529690.7629085093307657Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1338580.6675954424883319Illumina RNA PCR Primer (100% over 50bp)
AGCACACACAGTCTTTGCGAAGAAGAACAGCTCGGGATCGTCGGACTGTA1282020.6393870438665535No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA902420.4500675934276027No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC632440.3154193710105639No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA500950.24984082902368915No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG480320.239551945297252No Hit
AATTCAGCGGGTAGCCTTGCCTGATCTGAGGTCAAGATCGTCGGACTGTA441250.22006640544306386No Hit
AGCACACACAGTCTTTGCGAAGAAGAACAGCTCGGATCGTCGGACTGTAG404360.20166810584692874No Hit
CAGTTAAGAGCACACACAGTCTTTGCGAAGAAGAACAGCTCGGGATCGTC385300.19216223459991502No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG381020.19002765280887524No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG371540.18529965388853473No Hit
CGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTG371160.18511013494447046Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCAACATTAGAGTTTGGTATTGGCAGTGGATCGTCGGACTGTAGAACTC370710.18488570461597326Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG317550.15837300180950692No Hit
TCAGTTAAGAGCACACACAGTCTTTGCGAAGAAGAACAGCTCGGGATCGT302220.15072740861870315No Hit
AGAGCACTGCAAGCAGTCCACCGCATTCGACAACCGGCCGGAAGGAGATC290010.1446378657054798No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG287700.1434857900191943Illumina RNA PCR Primer (100% over 37bp)
AGTCTTTGCGAAGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCTGA283210.1412464740748558Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCACACTCAGTTAAGAGCACACACAGTCTTTGCGAAGAAGAACAGCTCG271620.1354661462808952No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC251220.12529197138902323Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC242380.12088316226921206No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC235620.11751172000112116No Hit
AGAACCGGTGTTCTCCCTGTGGTATGGTCGTAGGCGATCGTCGGACTGTA228350.11388592336073346No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT223250.11134237963776547Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTCTTTGCGAAGAAGAACAGCTCGGGATCGTCGGACTGTAGAACTCT216160.10780635512877663Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG205310.10239509054167806No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT205270.10237514117914498Illumina DpnII expression Sequencing Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)