FastQCFastQC Report
Wed 30 Apr 2025
1D5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20050766
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC5161262.574096171687406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG2413021.203455269489455No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1663990.8298884940355895No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1515640.7559012957410205No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTGGGGGG1283170.6399605880393796TruSeq Adapter, Index 2 (97% over 36bp)
CCGAGCTGTTCTTCTTCGCAAAGACTGTGTGTGCTAGATCGGAAGAGCAC1271790.6342849943987178No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC1099100.5481586090027682No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC892000.44487078448773476No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTGGGGGGGGGGG816460.40719641334400886TruSeq Adapter, Index 2 (96% over 32bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT625840.3121277261926053No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG491780.24526743766298004No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA474330.2365645282579229No Hit
TTGACCTCAGATCAGGCAAGGCTACCCGCTGAATTAGATCGGAAGAGCAC435030.21696427956916958No Hit
CGAGCTGTTCTTCTTCGCAAAGACTGTGTGTGCTAGATCGGAAGAGCACA400120.199553473418422No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTGTGTGTGCTCTTAACTGAGATCGG381220.19012739962154063No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC368140.18360395807322272No Hit
CACTGCCAATACCAAACTCTAATGTTGCTAGATCGGAAGAGCACACGTCT361950.18051679422122824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA341230.17018302442909164Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA314340.15677206546622707No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTGTGTGTGCTCTTAACTGAAGATCG300990.15011396572081087No Hit
TCCTTCCGGCCGGTTGTCGAATGCGGTGGACTGCTTGCAGTGCTCTAGAT293220.14623880204875964No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTAGATCGGAAGAGCACACGTCTGAA270900.13510705775529971Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAT262810.13107229918298383TruSeq Adapter, Index 2 (97% over 36bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT239910.11965128913279423Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT237050.11822490971167884No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG232410.1159107836578413No Hit
GCCTACGACCATACCACAGGGAGAACACCGGTTCTAGATCGGAAGAGCAC226370.11289842991534588No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC210610.10503838107731146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGCTGTTCTTCTTCGCAAAGACTGTAGATCGGAAGAGCACACGTCTG206820.10314817897730193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC202310.1008988883516969No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)