FastQCFastQC Report
Wed 30 Apr 2025
1D2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42652107
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT21778115.105986909392307Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA13022853.0532723740939693Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC10916412.5594069713836176No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA10041362.3542471184366107Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC7869941.8451468294403368No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT2908920.682010855876358Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC2591240.6075291895896257Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT2027570.47537393639193487No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT1886720.4423509488054131No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA1824800.4278334948376642No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1467860.3441471250177629No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1328390.31144768533943706Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1149920.2696045004294864No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA1131350.26525067096919736No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC971010.22765815531692254No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA925210.21692011604491193No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT911650.21374090616437777No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG895090.20985833126602632No Hit
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA874480.20502621359362153Illumina RNA PCR Primer (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG814370.19093312318662242No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT808100.18946309029938427Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT786560.18441292947145613Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG775140.18173545330363164No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT746420.17500190553306078No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT745700.1748330979287846Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG736430.17265970002372918No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT707670.16591677405292077No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG681460.159771708347257Illumina RNA PCR Primer (100% over 37bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC665660.1560673192534193No Hit
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT571790.13405902784591625No Hit
AATCATACATGACCAAAACGTGTTTCACCGATCGTCGGACTGTAGAACTC569270.13346820123094974Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT564040.13224200154988827No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGGGATCGT540090.12662680415764688No Hit
CCCTACCACTTGTACTTCTTTGATCGTCGGACTGTAGAACTCTGAACGTG524350.1229364823641655Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGA479910.11251730190023203No Hit
CGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTC464410.10888324930817604Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT462610.10846123029748565No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA462060.10833228004421916No Hit
AACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGTG456330.10698885286018812Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG454590.10658090114985411No Hit
GAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT448720.1052046502649916Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGAC443970.10409098898678087No Hit
GTCAAAGAATGGCAGAAAAAGGTCAAGCAGATCGTCGGACTGTAGAACTC442250.10368772637656563Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCAGAGATTGGTGAGCTGAGAGATCGTCGGACTGTAGAACTCTGAACGT440720.10332901021747883Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC433740.10169251427602392No Hit
TGTGTGAGGCAAGAATTTCTAACGTCTAAGATCGTCGGACTGTAGAACTC428940.10056713024751626Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC427630.10025999418973605Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)