FastQCFastQC Report
Wed 30 Apr 2025
1D2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42652107
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT11377732.667565754723442No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC10071312.361269045864487Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGG9803432.2984632388735218TruSeq Adapter, Index 4 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGGG8789802.060812611203475TruSeq Adapter, Index 4 (97% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT8201211.9228147392577815No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC2959390.6938438000261042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT2644390.6199904731552887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGGT2641770.6193762010397282TruSeq Adapter, Index 4 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGG2632910.6172989296871079TruSeq Adapter, Index 4 (97% over 45bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2102220.49287600258528846No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC1926350.45164240069077943No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG1848580.4334088348788959No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1525920.3577595826625869No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGGGGGGG1364110.3198224181515816TruSeq Adapter, Index 4 (97% over 41bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1353670.31737470788957745Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1187170.27833794940071777No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC1147390.2690113292644605No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG980010.2297682503703744No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG958030.2246149293398331No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG915370.21461307878647118No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC907760.21282887619127466No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC891470.2090096041445268No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG822910.19293536893734228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG821610.19263057742962147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA796770.18680671508209432No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC762900.1788657240309371No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC759500.17806857701074416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC726570.1703479736651697No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGTG704850.1652556109361725TruSeq Adapter, Index 4 (97% over 41bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT686930.16105417722974388TruSeq Adapter, Index 4 (100% over 41bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT682940.1601187017560469No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGGGGGGT621440.14569971889079242TruSeq Adapter, Index 4 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGTT597430.14007045419819472TruSeq Adapter, Index 4 (97% over 41bp)
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC582650.13660520921041486No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTAGATCGGAAGAG580610.1361269209982991No Hit
GGTGAAACACGTTTTGGTCATGTATGATTAGATCGGAAGAGCACACGTCT579400.1358432304411128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCG562100.13178715883836642No Hit
AAAGAAGTACAAGTGGTAGGGAGATCGGAAGAGCACACGTCTGAACTCCA527170.12359764548091376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCAGATCGGAAGAGCACAC491510.11523697996912556No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC474930.11134971597065532No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG474330.11120904296709187No Hit
TCAGGTCTAGGCTGGTTAGTTCAGATCGGAAGAGCACACGTCTGAACTCC463800.10874023175455319Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGGTCTAGGCTGGTTAGTTAGATCGGAAGAGCACACGTCTGAACTCCA461860.10828538904303134Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA461810.10827366629273438No Hit
TCTCAGCTCACCAATCTCTGCTAGATCGGAAGAGCACACGTCTGAACTCC451450.10584471243120534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTTGACCTTTTTCTGCCATTCTTTGACAGATCGGAAGAGCACACGTCT450640.10565480387639467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGA449620.10541565977033678No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT448010.10503818721077485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGAGATCGGAAGAGCACACGTCT441880.10360097802436818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA437330.10253420774734529No Hit
TTAGACGTTAGAAATTCTTGCCTCACACAAGATCGGAAGAGCACACGTCT436490.10233726554235645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGATCAATTGTCCGAAGCAACTGCAATAGATCGGAAGAGCACACGTC427920.1003279861414584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)