FastQCFastQC Report
Wed 30 Apr 2025
1D1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17347410
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1405480.8101958736203272Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1290480.7439035567845574Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATGCTTTCGCAGTAGTTCGTCTTTCGGATCGTCGGACTGTAGAACTCTG1206820.6956773374238575Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAAGTAAACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACT727540.4193940190495296No Hit
AGTAAACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCT606070.34937203882308654Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC538610.31048438931229505Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCACACACAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT524460.3023275520668503Illumina RNA PCR Primer (100% over 31bp)
GTAAACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTG469380.2705764145771617Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCACACACAGTCTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT452230.2606902125446969Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT438160.2525794916935727Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATGCTTTCGCAGTAGTTCGTCTTTCGGATCGTCGGACTGTAGAACTCTGA418630.24132132693007197Illumina DpnII expression Sequencing Primer (100% over 24bp)
CACACACAGTCTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT412020.23751095984933776Illumina RNA PCR Primer (100% over 31bp)
AACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAAC375850.21666058506716568Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTG357620.20615181171137362Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTGT325010.18735361647646537Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGCTTTCGCAGTAGTTCGTCTTGATCGTCGGACTGTAGAACTCTGAAC316530.1824652786784886Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCTTCAATTAAGCAAGTCGGACATCTGATCGTCGGACTGTAGAACTCTG310840.17918525013244052Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG301440.17376657379977758No Hit
GTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGTG296240.1707690081689428Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGAGCGCCATTTGCGTTCAAAGATTCAATGATCGTCGGACTGTAGAACT284070.16375355168293135No Hit
GAGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA269700.15546989435310515Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGCTTTCGCAGTAGTTCGTCTTTGATCGTCGGACTGTAGAACTCTGAA264720.15259914880665185Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAGAGCGCCATTTGCGTTCAAAGATTCAAGATCGTCGGACTGTAGAACTC260700.1502817999920449Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTTCAATTAAGCAAGTCGGACATCGATCGTCGGACTGTAGAACTCTGA257150.14823538499407116Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAAGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTC248490.1432432853088732Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCTTCAATTAAGCAAGTCGGACATCTTGATCGTCGGACTGTAGAACTCT246130.1418828516764174Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGGATCGTCGGA240070.13838953480663685No Hit
TCACAGATCCTGAGCACACTCAGTTAAGGATCGTCGGACTGTAGAACTCT234110.13495386342975696Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGCTTTCACCGAAGTTCGCAGGATCGTCGGACTGTAGAACTCTGAACG231610.13351272610724021Illumina Small RNA Adapter 1 (100% over 26bp)
TAAACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGA222060.1280075815352263Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTGA220430.1270679600009454Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAACG210720.1214705826402904Illumina Small RNA Adapter 1 (100% over 26bp)
TCTCAGAAGATCGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT203390.11724516801067132Illumina RNA PCR Primer (100% over 31bp)
GTAAACGTCACAGATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAG202940.11698576329261832Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTGAACG202340.1166398903352143Illumina Small RNA Adapter 1 (100% over 26bp)
AAGAGCGCCATTTGCGTTCAAAGATTCAATGATTCACTGAGATCGTCGGA197130.11363656015508942No Hit
CGTCACAGATCCTGAGCACACTGATCGTCGGACTGTAGAACTCTGAACGT195410.11264505767719792Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATGCTTTCGCAGTAGTTCGTCTTGATCGTCGGACTGTAGAACTCTGAACG195400.11263929312790785Illumina Small RNA Adapter 1 (100% over 26bp)
GCACACACAGTCTTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC186170.10731861413317609Illumina RNA PCR Primer (100% over 30bp)
GCTTCAATTAAGCAAGTCGGACATCGATCGTCGGACTGTAGAACTCTGAA186140.10730132048530588Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCACAGATCCTGAGCACACTCAGATCGTCGGACTGTAGAACTCTGAACGT183630.10585441861349906Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCACACACAGTCTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC182160.10500702986785924Illumina RNA PCR Primer (100% over 30bp)
GAGCACACTCAGTTAAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG181420.10458045322039428Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACACACAGTCTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC180590.10410199562931872Illumina RNA PCR Primer (100% over 32bp)
TCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTGAACGTGTA178110.10267238740538213Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTTCAATTAAGCAAGTCGGACATCTGATCGTCGGACTGTAGAACTCTGA178060.10264356465893179Illumina DpnII expression Sequencing Primer (100% over 24bp)
CACACACAGTCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG177450.1022919271522377Illumina RNA PCR Primer (100% over 33bp)
AAGAGCGCCATTTGCGTTCAAAGATTCAATGGATCGTCGGACTGTAGAAC177140.10211322612424564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)