FastQCFastQC Report
Wed 30 Apr 2025
1D1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17347410
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1259390.7259815730417394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGG1240430.7150519875877724TruSeq Adapter, Index 4 (97% over 45bp)
CGAAAGACGAACTACTGCGAAAGCATTAGATCGGAAGAGCACACGTCTGA1193800.6881718942481904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTC718850.4143846257164614No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTAGATCGGAAGAGCACACGTCTG595320.3431751483362646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT534040.30784999028673443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACTGTGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG512320.29532938922870905TruSeq Adapter, Index 4 (100% over 35bp)
AGTGTGCTCAGGATCTGTGACGTTTACAGATCGGAAGAGCACACGTCTGA466480.2689046952830423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGACTGTGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC442880.2553003589584843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTAACTGAGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC429080.2473452809381919Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAAAGACGAACTACTGCGAAAGCATAGATCGGAAGAGCACACGTCTGAA414960.2392057373406174Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGACTGTGTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG397190.22896213325216846TruSeq Adapter, Index 4 (100% over 35bp)
AGTGTGCTCAGGATCTGTGACGTTAGATCGGAAGAGCACACGTCTGAACT370260.21343820201401822Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGCTCAGGATCTGTGACGTTTACTAGATCGGAAGAGCACACGTCTGA350320.20194369072962476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGCTCAGGATCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAG318150.18339913566347946Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGACGAACTACTGCGAAAGCATTAGATCGGAAGAGCACACGTCTGAACT312980.18041886368051485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATGTCCGACTTGCTTAATTGAAGCCAGATCGGAAGAGCACACGTCTGA309860.17862032430201397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC295870.1705557198452103No Hit
AGTGTGCTCAGGATCTGTGACAGATCGGAAGAGCACACGTCTGAACTCCA292380.16854389214297696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC289750.16702781567968936No Hit
ATTGAATCTTTGAACGCAAATGGCGCTCTTAGATCGGAAGAGCACACGTC282160.16265252276852854No Hit
CTTAACTGAGTGTGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA267770.15435733634012225Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGACGAACTACTGCGAAAGCATTAGATCGGAAGAGCACACGTCTGAAC262040.15105424959691388Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCTTTGAACGCAAATGGCGCTCTTAGATCGGAAGAGCACACGTCT257850.14863890344437586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCCGACTTGCTTAATTGAAGCCAGATCGGAAGAGCACACGTCTGAA256190.14768198826222473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTCT245030.14124875125451003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATGTCCGACTTGCTTAATTGAAGCCAGATCGGAAGAGCACACGTCTG244500.14094323014213647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAAG237250.13676393190683797No Hit
CTTAACTGAGTGTGCTCAGGATCTGTGAAGATCGGAAGAGCACACGTCTG230520.13288439023462292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCGAACTTCGGTGAAAGCTGAAGATCGGAAGAGCACACGTCTGAACTC227950.13140290106707572Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTGCTCAGGATCTGTGACGTTTACAGATCGGAAGAGCACACGTCTGAA218010.12567293907274918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGCTCAGGATCTGTGACGTTTAAGATCGGAAGAGCACACGTCTGAA217150.12517718783380344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTGCTCAGGATCTGTGACGTAGATCGGAAGAGCACACGTCTGAACTC207940.1198680379376518Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGATCTTCTGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG198870.11463959173156107TruSeq Adapter, Index 4 (100% over 35bp)
GTGTGCTCAGGATCTGTGACGTTAGATCGGAAGAGCACACGTCTGAACTC198830.11461653353440082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGATCTGTGACGTTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTC198530.11444359705569881Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGTGAATCATTGAATCTTTGAACGCAAATGGCGCTCTTAGATCGGAAG194720.1122473037761833No Hit
AAGACGAACTACTGCGAAAGCATAGATCGGAAGAGCACACGTCTGAACTC194230.11196484086097003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGTCCGACTTGCTTAATTGAAGCAGATCGGAAGAGCACACGTCTGAAC183520.10579100857130834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGACTGTGTGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT183410.1057275985291176Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGCTCAGGATCTGTGACGAGATCGGAAGAGCACACGTCTGAACTCC182280.10507620445934004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGTGTGCTCAGGATCTGTGAAGATCGGAAGAGCACACGTCTGAACTCC179370.10339872061593057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTAACTGAGTGTGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC179240.10332378147515969Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACTGTGTGTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT178720.10302402491207621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTGAATCTTTGAACGCAAATGGCGCTCTTAGATCGGAAGAGCACACGT176030.10147336115304821No Hit
AGATGTCCGACTTGCTTAATTGAAGCAGATCGGAAGAGCACACGTCTGAA175820.1013523056179568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGACTGTGTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA173990.10029739309787455TruSeq Adapter, Index 4 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)