FastQCFastQC Report
Wed 30 Apr 2025
1D12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314677
Total Bases47.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA13745043.67970967055108Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3530911.220712031702348Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT183855.842498816246501Illumina RNA PCR Primer (100% over 50bp)
AATTCAAATCCCTTCCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG66612.1167737076430755Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC23320.7410773586884329No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG16410.5214871121816974Illumina RNA PCR Primer (100% over 37bp)
TTCCGCTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA15900.5052800172875679Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT14050.446489575024549No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC10830.3441624268694566Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT8230.2615380215268354Illumina RNA PCR Primer (100% over 44bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT6240.19829857282229077No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG6030.19162506316000216No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA5190.16493102451084762No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG4900.15571522545340144No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG4670.1484061434423234Illumina Small RNA Adapter 1 (100% over 26bp)
AATTCAAATCCCTTCCAAAGATCGTCGGACTGTAGAACTCTGAACGTGTA4420.14046148908245598Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4070.1293389729786416Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGGTTTTCAGATCGTCGGACTGTAGAACTCTGAA3930.12488996653711583Illumina DpnII expression Sequencing Primer (100% over 25bp)
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3720.11821645687482721Illumina RNA PCR Primer (100% over 45bp)
CCAAGACCACAAAGGTACCAGACATCGGAGATCGTCGGACTGTAGAACTC3640.11567416747966963Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA3560.11313187808451207Illumina RNA PCR Primer (100% over 49bp)
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT3370.10709394077101282Illumina RNA PCR Primer (100% over 35bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG3310.10518722372464463No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC3230.10264493432948707No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT3200.10169157580630296Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)