FastQCFastQC Report
Wed 30 Apr 2025
1D12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314677
Total Bases47.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGG10649633.84295642833763TruSeq Adapter, Index 22 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3357810.67062416382513Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTAGGGGG185475.893980176498441TruSeq Adapter, Index 22 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGGGGGG78612.4981171169167116TruSeq Adapter, Index 22 (97% over 39bp)
TTGGAAGGGATTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC63322.01222205626722Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGTG31841.0118311792727146TruSeq Adapter, Index 22 (97% over 43bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG23270.7394884278164594No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGGGGGT22390.711523244469726TruSeq Adapter, Index 22 (97% over 39bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAT15690.49860650762527925TruSeq Adapter, Index 22 (97% over 41bp)
TGAAAATCTTTGCTCTGAAGCGGAAAGATCGGAAGAGCACACGTCTGAAC15230.48398834360312315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG13980.4442650718037861No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTAGGGTG10490.3333576969400369TruSeq Adapter, Index 22 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT10230.3250952564057748Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGG8850.28124076433930667TruSeq Adapter, Index 22 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTAGGGGGGGGGG7550.23992856166799606TruSeq Adapter, Index 22 (97% over 39bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGG6660.21164559214686807TruSeq Adapter, Index 22 (97% over 43bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC6630.21069223362368397No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA5980.19003613228802868No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC5970.18971834611363397No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGGGGTG5700.18113811940497715TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGG5320.16906224477797868TruSeq Adapter, Index 22 (97% over 35bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG5180.16461323833645294No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGGGGTT4950.1573041563253749TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGG4520.14363935082640295TruSeq Adapter, Index 22 (96% over 32bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC4310.1369658411641143Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGAAGGGATTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGT4270.13569469646653554Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGT4250.13505912411774615TruSeq Adapter, Index 22 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACCGTACGATCTGGGGGGG4160.13219904854819386Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGAAAACCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3800.12075874626998478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGATGTCTGGTACCTTTGTGGTCTTGGAGATCGGAAGAGCACACGTCT3510.11154294721253857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGG3470.11027180251495979TruSeq Adapter, Index 22 (97% over 39bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA3330.10582279607343403No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTAGGGGGGGGGT3200.10169157580630296TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGGCACACGTCTGAACTCCAGTCACCGTACGATCTGGGGGGG3160.10042043110872417TruSeq Adapter, Index 22 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)