FastQCFastQC Report
Wed 30 Apr 2025
1C6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145390
Total Bases21.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGG104547.1903157025930255TruSeq Adapter, Index 13 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTAGGGGG41072.8248160121053716TruSeq Adapter, Index 13 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGGGGGG16031.1025517573423207TruSeq Adapter, Index 13 (97% over 39bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC6740.46358071394181166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTAGGGGGGGGGG4180.28750257926955086TruSeq Adapter, Index 13 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGGGGGGGT3380.23247816218446934TruSeq Adapter, Index 13 (97% over 39bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1780.12242932801430635No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.11417566545154413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)