FastQCFastQC Report
Wed 30 Apr 2025
1C5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39214111
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT19557824.98744444314956Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA16650474.246040411320302Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA11537332.9421373341856456Illumina RNA PCR Primer (100% over 45bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC8328862.1239446177933243No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC6211901.5840981324299306No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT4119931.05062435305495Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT3156270.8048811816746273No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC2185300.5572738854133401Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1705300.4348689684690289No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA1521730.3880567380451389No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1518370.38719990362652873Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT1497740.3819390423003597No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1299420.33136541078286846No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG1220860.3113318060429829No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1076650.27455677880852636No Hit
TAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC1030850.26287730965009No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT938200.23925061057740157Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT928930.23688666561891458Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT900760.2297030270557453Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG900070.22952706998763786No Hit
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT878510.22402904913488925No Hit
CGCTCTTCTAGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACTC785910.20041510057438253Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT772340.19695461156826938No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA737450.18805730416787977No Hit
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT725170.18492577837605448No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG717440.1829545491927638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG699520.17838476562684286No Hit
GAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT687920.17542664680068867Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGGGATCGT652370.1663610326395006No Hit
AACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGTG640140.16324225735985703Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC628880.16037084201653837No Hit
GGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT621800.1585653694916098No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA599640.15291434249268077No Hit
TGTGTGAGGCAAGAATTTCTAACGTCTAAGATCGTCGGACTGTAGAACTC594600.1516290908647655Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAATGTTTCAGATGAATTGAGCGATATGAGATCGTCGGACTGTAGAACT571360.14570265280271177No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC566640.14449900445275937No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA563610.1437263234145484No Hit
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA559100.14257622721575916Illumina RNA PCR Primer (100% over 49bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT523850.1335871161276613Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG509990.1300526741508943No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT509460.1299175187217683No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC500910.12773718113869775Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCT476360.12147667965748352Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACTCATTTAGCTAGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTC439650.11211525361368004No Hit
GAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGA431990.11016187514744373No Hit
AAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG419810.10705585037998183No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGAC414340.10566094434730396No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGGGATCGTC406990.10378661905659418No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA406110.1035622100421963No Hit
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT404150.10306238996467369No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC401300.10233561077031685Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCAGCCGGGAAACGAACCCGGGTCACATCGATGGCAACGGATCGTCGGA400120.10203469868282873No Hit
TAGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAAC399740.10193779479024782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)