FastQCFastQC Report
Wed 30 Apr 2025
1C5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39214111
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC16750104.271447081893556Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8735702.2276929853133733No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATGCCGTC6820081.7391902624032456TruSeq Adapter, Index 11 (98% over 50bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT6478541.6520940637924955No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATG5716441.4577507571190382TruSeq Adapter, Index 11 (97% over 49bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC4211781.0740470439327312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC3218220.8206790662677524No Hit
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT2235570.5700932503608204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1777950.45339546266903763No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1555930.39677808837742107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG1546460.3943631413702073No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC1528360.3897474559604322No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTGTG1479690.3773361074027663TruSeq Adapter, Index 11 (97% over 46bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC1351160.34455964078849066No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTGTGCCGTC1257870.3207697351598765TruSeq Adapter, Index 11 (97% over 42bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC1248260.3183190867185539No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1119040.28536666303617086No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGATG1069710.27278700771770653TruSeq Adapter, Index 11 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT1044280.26630209722209436No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATGTCGTC1043040.2659858845199882TruSeq Adapter, Index 11 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGATGCCGTC1008040.2570605259927988TruSeq Adapter, Index 11 (97% over 41bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG977120.24917560925963614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC950170.24230308319370034Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG930640.23732273313552868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA924870.2358513240297606No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTAGATCGGAAGAG896560.2286319840324826No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGTG840190.21425705659883504TruSeq Adapter, Index 11 (97% over 45bp)
AGCTGTCGACTCCTGCACCTAGAAGAGCGAGATCGGAAGAGCACACGTCT796350.2030774075179213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC782940.1996577201507896No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC751200.19156369501784704No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC744770.1899239791512805No Hit
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA732080.18668789915956527No Hit
TCAGGTCTAGGCTGGTTAGTTCAGATCGGAAGAGCACACGTCTGAACTCC714110.18210536508146263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCG682350.17400623974364737No Hit
TCAGGTCTAGGCTGGTTAGTTAGATCGGAAGAGCACACGTCTGAACTCCA650240.16581786082056024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACCAGATCGGAAGAGCACACGTC642040.16372677682276157No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG634660.1618448012247428No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG621260.15842766396004745No Hit
TTAGACGTTAGAAATTCTTGCCTCACACAAGATCGGAAGAGCACACGTCT606240.15459741010066505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATATCGCTCAATTCATCTGAAACATTTCAGATCGGAAGAGCACACGTC588200.149997025305508No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT583350.1487602256238832No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC574820.14658498824568533No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGTATGCCGTC570890.14558279798820378TruSeq Adapter, Index 11 (98% over 50bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC561770.14325710456626187No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG534430.1362851245053088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT525860.13409968671736558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG511100.13033573552132802No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTGTGTCGTC499790.1274515696658277TruSeq Adapter, Index 11 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGGGGG492970.12571239980424395TruSeq Adapter, Index 11 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCTAAGATCGGAAGAGCACACGTCTG480780.1226038249343457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGCTAAATGAGTTAGATCGG453350.11560889395146558No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCAGATCGGAAGAGCACAC443330.11305369131025308No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTAGATCGGAAGAGCACA428690.1093203413434516No Hit
CCACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGG424850.10834110200789711No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGA421260.10742561523325111No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG417850.10655603030245923No Hit
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC417490.10646422661475101No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTGGTATG407550.10392942479302922TruSeq Adapter, Index 11 (97% over 49bp)
GAGGTCCGGATTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT405010.1032816987741989No Hit
CGTTGCCATCGATGTGACCCGGGTTCGTTTCCCGGCTGATAGATCGGAAG403860.10298843699401983No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT402300.10259062101395083Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCCTCCCCGCTCTTAGACCTCACTAGATCGGAAGAGCACACGTCTGA399130.10178223854163108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAT398220.10155017921992418TruSeq Adapter, Index 11 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)