FastQCFastQC Report
Wed 30 Apr 2025
1C12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243794
Total Bases336.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT814793.631304834579289Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA542722.418760367484716Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC505862.2544850373964813No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA457712.0398931452709115Illumina RNA PCR Primer (100% over 45bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC371761.6568365901682598No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT116410.5188087676497931No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT107950.4811047716501604No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG107190.4777176514421556No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT92620.41278299166501026Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA86960.3875578595896058No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT80210.35747488405798394No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT74490.33198234775563173No Hit
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT53430.23812346409697147No Hit
ATGGATCGGAGTGGTTCCACTACGTCATAGACACCAGGAGATCGTCGGAC52590.23437980491970295No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT45340.20206846083018318Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT39680.17684332875477873No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC39470.1759074139604616Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37630.16770701766739726No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG35170.15674344436253954Illumina RNA PCR Primer (100% over 37bp)
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA33920.15117252296779474No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC33280.14832021121368538Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT33030.14720602693473644Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC32290.1439080414690475No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA31260.1393176022397778No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA30970.138025148476197No Hit
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA30610.1364207231145105Illumina RNA PCR Primer (100% over 49bp)
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC30190.13454889352587626No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT29430.13116177331787143Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT28690.12786378785218253No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC27090.12073300846690918No Hit
AATCTCTTTCAGGTGCAGGAATAGACAGCAGATCGTCGGACTGTAGAACT26780.11935141996101245No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG25910.11547405867027008No Hit
ACCGCGGTCCATTCAGATCCGCAAGCCCCGGACCGCCACAACGATCGTCG25470.11351309433931991No Hit
ACGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT24180.1077639034599433No Hit
AATATCAATTTATCAGTCTGGGATAGCCACAGATCGTCGGACTGTAGAAC24010.107006258150258No Hit
AGACCGCGGTCCATTCAGATCCGCAAGCCCCGGACCGCCACAACGATCGT23830.10620404546941475No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA22760.10143533675551321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)