FastQCFastQC Report
Wed 30 Apr 2025
1C12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243794
Total Bases336.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT516762.303063471958656No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC516132.300255727575704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT381911.7020724718935873No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGG217680.9701425353664374TruSeq Adapter, Index 21 (97% over 43bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC120560.5373042266803458No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC115110.5130150093992586No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC110260.49139983438764884No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC92260.4111785663033237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG89260.39780835495593625No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC82290.3667448972588393No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGGGGG79370.35373122488071546TruSeq Adapter, Index 21 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTAGGGGG76980.3430796231739634TruSeq Adapter, Index 21 (97% over 43bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC75580.3368401912118492No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGTG71650.3193252143467716TruSeq Adapter, Index 21 (97% over 43bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC53290.23749952090076007No Hit
TCCTGGTGTCTATGACGTAGTGGAACCACTCCGATCCATAGATCGGAAGA53020.23629620187949518No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC42940.19137229175227316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC39800.17737813720867424No Hit
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT39760.1771998677240424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTAGGGTG39480.17595198133161957TruSeq Adapter, Index 21 (97% over 43bp)
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC34910.15558469271243258No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGAT34630.1543368063200098TruSeq Adapter, Index 21 (97% over 41bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT33160.14778540275978988Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG32920.1467157858519989Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG32660.14555703420189198No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC31850.14194707713809734No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGCGTT31640.14101116234378022TruSeq Adapter, Index 21 (97% over 39bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC31520.14047635388988472No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT30180.1345043261547183No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGGGGT29630.1320531207410306TruSeq Adapter, Index 21 (97% over 39bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG29440.1312063406890294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG28690.12786378785218253No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT27510.12260483805554341No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGGGGCGGT27280.1215797885189104TruSeq Adapter, Index 21 (97% over 39bp)
TGCTGTCTATTCCTGCACCTGAAAGAGATTAGATCGGAAGAGCACACGTC27090.12073300846690918No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC25770.11485011547405868No Hit
GTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGACCGCGGTAGATCGGA25540.11382506593742563No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC24570.10950203093510368No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGTGGCGTT23960.1067834212944682TruSeq Adapter, Index 21 (97% over 39bp)
GTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGACCGCGGTCTAGATCG23900.10651601706752045No Hit
TGTGGCTATCCCAGACTGATAAATTGATATTAGATCGGAAGAGCACACGT23860.10633774758288862No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTGGGGTGTCGTT22920.10214841469404054TruSeq Adapter, Index 21 (97% over 39bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG22870.10192557783825075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)