FastQCFastQC Report
Wed 30 Apr 2025
1C11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323
Total Bases648.4 kbp
Sequences flagged as poor quality0
Sequence length150
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA284465.78764746703678Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT46510.756419153365718Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA561.2953967152440435Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA250.5783021050196623No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG220.5089058524173028Illumina RNA PCR Primer (100% over 37bp)
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA160.3701133472125839Illumina RNA PCR Primer (100% over 49bp)
CTACGCATTTCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG90.2081887578070784Illumina RNA PCR Primer (100% over 33bp)
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT90.2081887578070784Illumina RNA PCR Primer (100% over 35bp)
NATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA80.18505667360629194Illumina RNA PCR Primer (100% over 45bp)
CTTGGACTAGAGCTTTAATAAAAAAATCTTAAATTCTTTGAAATTCAAGT60.13879250520471895No Hit
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA60.13879250520471895Illumina RNA PCR Primer (100% over 45bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA50.11566042100393245No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCAGTA50.11566042100393245Illumina RNA PCR Primer (97% over 45bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT50.11566042100393245Illumina RNA PCR Primer (100% over 44bp)
GATCGTCGGACTGTAGACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT50.11566042100393245Illumina RNA PCR Primer (97% over 35bp)
CTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG50.11566042100393245Illumina RNA PCR Primer (100% over 43bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50.11566042100393245No Hit
TGCAGACGAGTCCCGCATCACTTTCTTGAAAGGGGAGAAGGTGAAAAACT50.11566042100393245No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCNCCGTA50.11566042100393245Illumina RNA PCR Primer (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)