FastQCFastQC Report
Wed 30 Apr 2025
1C11_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C11_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4323
Total Bases648.4 kbp
Sequences flagged as poor quality0
Sequence length150
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG63814.75826972010178TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGG44410.270645385149201TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGTG3437.9343048808697665TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTG2806.476983576220217TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTG2215.1121906083738144TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGTG2154.973398103169095TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGGG1212.7989821882951653TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTATG1072.475133009484155TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGGG861.9893592412676382TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGAGG651.5035854730511218TruSeq Adapter, Index 20 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC571.31852879944483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGATG551.2722646310432568TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTATG380.8790191996298867TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGAGG350.8096229470275271TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGATG260.6014341892204488TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGG260.6014341892204488TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTAGG250.5783021050196623TruSeq Adapter, Index 20 (97% over 43bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG240.5551700208188758No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCAT200.4626416840157298TruSeq Adapter, Index 20 (97% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGTGTCGTT110.2544529262086514TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTAGG110.2544529262086514TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGTG110.2544529262086514TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGGGCGTT110.2544529262086514TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGTGTCGTC90.2081887578070784TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGTGGCGTT90.2081887578070784TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTGTCGTC90.2081887578070784TruSeq Adapter, Index 20 (97% over 39bp)
TGAAATGCGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG90.2081887578070784TruSeq Adapter, Index 20 (100% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGCGTT90.2081887578070784TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTGGCGTT90.2081887578070784TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGTGTCGTT80.18505667360629194TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTGTCGTT80.18505667360629194TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGTGGCGTC80.18505667360629194TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGT80.18505667360629194TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGCGGG70.16192458940550544TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGCGGT70.16192458940550544TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGTT70.16192458940550544TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTGTCGTT70.16192458940550544TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTGGCGTC70.16192458940550544TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGTGCCGTC60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGTGTCGTC60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGGGGCGTT60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
GCCAGTTTGACAACCTGAATTAGAAACTTAAGTCTGTAAAAAAATCTTAA60.13879250520471895No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGGGCGGT60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTGGCGTT60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACGTGGCCATCTGGGGGG60.13879250520471895TruSeq Adapter, Index 20 (97% over 39bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG50.11566042100393245No Hit
GCTGAATTTGAGCACTCGACTCTTGGGGTAGTAATCCACTCCGGCCTGAG50.11566042100393245No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTGGT50.11566042100393245TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGGGCGGC50.11566042100393245TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTT50.11566042100393245TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGTATGCCGTC50.11566042100393245TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTGGCGTC50.11566042100393245TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGGTCGGT50.11566042100393245TruSeq Adapter, Index 20 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)