FastQCFastQC Report
Wed 30 Apr 2025
1B7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269760
Total Bases40.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC74

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA14183152.57673487544484Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA239718.886046856465006Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT139675.177565243179122Illumina RNA PCR Primer (100% over 50bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC27691.0264679715302492Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG19450.7210112692763938Illumina RNA PCR Primer (100% over 43bp)
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA13980.5182384341637011Illumina DpnII expression Sequencing Primer (100% over 24bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG12700.47078884934756815Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC11570.4288997627520759No Hit
AGGCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC9940.36847568208778175Illumina RNA PCR Primer (100% over 39bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT7360.27283511269276395No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC7140.2646797153024911Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA6180.22909252669039143Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC5570.20647983392645314Illumina RNA PCR Primer (100% over 41bp)
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA4080.15124555160142347Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT3560.1319691577698695Illumina RNA PCR Primer (100% over 35bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG3350.12418446026097271No Hit
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3350.12418446026097271Illumina RNA PCR Primer (100% over 45bp)
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA3100.11491696322657177No Hit
GATCGTCGGACTGTAGACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT3000.1112099644128114Illumina RNA PCR Primer (97% over 35bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT2910.10787366548042705Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT2820.10453736654804271Illumina RNA PCR Primer (100% over 37bp)
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG2810.10416666666666667No Hit
TTCCACTTCAGAGCCAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2800.10379596678529061Illumina DpnII expression Sequencing Primer (100% over 25bp)
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2720.10083036773428232Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)