FastQCFastQC Report
Wed 30 Apr 2025
1B6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512999
Total Bases376.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA970933.8636306659891226Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC564442.246081275798359No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA446721.7776369986617584Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC416331.6567057925609998No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT279971.1140871922352535No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA239160.9516915844375584No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT208410.8293278270305718Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG156190.6215283014438128No Hit
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT132850.5286512250900219No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG117990.46951869061627166No Hit
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA102240.4068445709687907No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC91690.36486285907793836No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT87240.34715493320928503No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG82770.32936742115695233No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA76620.304894669675555No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA70990.2824911589698205No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA68470.2724632998262236No Hit
AGAACCGGTGTTCTCCCTGTGGTATGGTCGTAGGCGATCGTCGGACTGTA67080.26693206006050935No Hit
ACCACCAAAGAGTCTAGAATTGTACATGATCGTCGGACTGTAGAACTCTG65740.26159978575399356Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACACATCCACTAGCGGTAGGTTGCCAGGATCGTCGGACTGTAGAACTCTG59000.23477924185405563Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGGATCGTCGGACTGTAGA55730.22176690082248343No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC53620.21337055844431294No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACGATCGTCGGACTGTAG49990.19892566610651258No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA47310.1882611174934809No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA46350.18444098067687253No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC45360.18050146458474517No Hit
AATTCAAATCCCTTCCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG44330.1764027761252591Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT42740.1700756745227515Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCTTCAGACGGCCATCGATCACGCACAAGCGGATCGTCGGACTGTAGAA41610.16557905514486873No Hit
AGCCGACAGTACCCTAACCGATCGTCGGACTGTAGAACTCTGAACGTGTA40730.16207726306297776Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC40290.16032636702203223No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT40150.15976926373627687No Hit
GTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGAA37290.1483884394701311No Hit
AGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT36960.14707526743942198No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG36300.14444892337800375No Hit
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT34290.13645051191822996Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTTGAGAATAGGTCGAAGACAACACGCGATCGTCGGACTGTAGAACT33510.13334665075473567No Hit
AACTTATGAGTCAAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCG30490.1213291370191552No Hit
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGAGATCGTCGGACTGTAG30310.12061286136604112No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG30080.11969762025372871No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG29190.11615603507999805No Hit
AAGCCTACAACACGGGGTATTCCCAGATCGTCGGACTGTAGAACTCTGAA28560.11364907029409879Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCTTCATAAATTCCATCATCAACACTAATTGATCGTCGGACTGTAGAA28280.11253486372258803No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG27770.11050541603876483Illumina RNA PCR Primer (100% over 37bp)
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGAC26610.10588941738536306No Hit
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC26500.10545169337512669Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA25950.10326307332394483No Hit
AGACAACTACTCACGCTTAAGCACACAGCTTGCGGATCGTCGGACTGTAG25580.10179072892587701No Hit
ACAGGATTCTCACCCGGGCACACGCTCTATTCCAAGGATCGTCGGACTGT25430.10119383254828195No Hit
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC25250.10047755689516788No Hit
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT25200.10027859143596954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)