FastQCFastQC Report
Wed 30 Apr 2025
1B6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512999
Total Bases376.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG563912.243972241930856No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGG485831.9332677808467096TruSeq Adapter, Index 12 (97% over 45bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC432161.719698256943198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT423111.6836855088282965No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGGGGG362041.440669096963429TruSeq Adapter, Index 12 (97% over 45bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG278991.1101874692349658No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC238690.949821309121094No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA155650.6193794744844706No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA133720.5321132240800732No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC118170.4702349662693857No Hit
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC104900.417429533398143No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT91890.3656587209147318No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC87320.34747327794400235No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA82870.329765352075349No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC78590.3127339087679701No Hit
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG76620.304894669675555No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG71150.28312784843925526No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG69110.2750100577039625No Hit
GCCTACGACCATACCACAGGGAGAACACCGGTTCTAGATCGGAAGAGCAC67200.26740957716258545No Hit
ATGTACAATTCTAGACTCTTTGGTGGTAGATCGGAAGAGCACACGTCTGA66030.262753785417344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGCAACCTACCGCTAGTGGATGTGTAGATCGGAAGAGCACACGTCTGA58740.23374462146622424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACAC55530.22097103898568998No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT53750.21388786863822867No Hit
GTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCACA50050.1991644246575506No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG47510.18905697933027432No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC46380.18456035995239153No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG45100.17946684419691372No Hit
TTGGAAGGGATTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC43250.1721051222065747Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG42180.16784726137972994Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTTGTGCGTGATCGATGGCCGTCTGAAGCTAGATCGGAAGAGCACACG41500.16514133113463236No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC40330.16048553938939092No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA40130.15968967755259753No Hit
GGTTAGGGTACTGTCGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT39990.15913257426684213Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTGGGGGGGGGGG37860.15065664570499232TruSeq Adapter, Index 12 (97% over 41bp)
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG37310.14846802565381045No Hit
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCTAGATCGGAAGAGCA36620.1457223023168732No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGC35890.14281740661257725No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTTAGATCGGAAGAGCACACGTC33490.13326706457105633No Hit
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC32830.1306407205096381Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTGACTCATAAGTTAGATC31150.12395548108057346No Hit
TCTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACA30350.12077203373339981No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC29070.115678517977922No Hit
AATTAGTGTTGATGATGGAATTTATGAAGGCTAGATCGGAAGAGCACACG28520.11348989792674012No Hit
TGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAAC28420.11309196700834342Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT27290.10859534763046066TruSeq Adapter, Index 12 (100% over 41bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT26610.10588941738536306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGTGGG26580.10577003810984405TruSeq Adapter, Index 12 (97% over 46bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGA26580.10577003810984405No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG26260.10449665917097459No Hit
CGCAAGCTGTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACA25910.10310390095658614No Hit
CTTGGAATAGAGCGTGTGCCCGGGTGAGAATCCTGTAGATCGGAAGAGCA25460.10131321182380096No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA25390.10103466018092327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)