FastQCFastQC Report
Wed 30 Apr 2025
1B4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21471113
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT6115432.848212852309985Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA4185551.9493866014304893Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1383250.6442376787826509No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC985080.4587931701537783No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT966210.45000461783234064No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA896060.41733281362731406Illumina RNA PCR Primer (100% over 45bp)
ACGGGATCGGTCACGGTAAGATCGTCGGACTGTAGAACTCTGAACGTGTA829130.38616069879563303Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC546100.2543417288148966No Hit
ACTCACTCACGCCACAGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA496530.23125489582212158Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA480510.22379370831870712No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG412400.19207201787816028No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT389210.18127145993782437No Hit
CATCGCACACCTATGCTGTCGGAGTTGGGATCGTCGGACTGTAGAACTCT388750.18105721859877502Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC378530.17629733493554806Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACATCGCACACCTATGCTGTCGGAGTTGGGATCGTCGGACTGTAGAACTC364480.16975365925371452Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCACTACAGCACCCCGGATTCGCGGATTGATCGTCGGACTGTAGAACTCT333980.1555485269906595Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCGCTCTCCTCAGTGATGAACCGACATGATCGTCGGACTGTAGAACTC316220.14727694833518876Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC305330.142205017504216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297440.13853031279747816No Hit
TAAATTCAGCGGGTCGCCTTACTTCATTTGAGATCAGAGATCGTCGGACT280820.1307896800692167No Hit
TTCCACTTCAGAGCCAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA276830.12893136932398427Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG275720.12841439565801735No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT273910.1275714025630623Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG269090.1253265259234582Illumina RNA PCR Primer (100% over 37bp)
ACGGGATCGGTCACGGTAACGATCGTCGGACTGTAGAACTCTGAACGTGT258150.12023130799041484Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATTCACTAATCGCTACTCGGATCGTCGGACTGTAGAACTCTGAACGTGT246330.11472623706092926Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG220300.10260297172298427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)