FastQCFastQC Report
Wed 30 Apr 2025
1B4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21471113
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3880081.8071163800404757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1348900.6282394396601612No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG977890.45544448487602857No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG956220.4453518548386383No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC907500.42266090258106326No Hit
TTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT775610.36123418473928204Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTAGGGGGGGGGG648510.30203837127586264TruSeq Adapter, Index 1 (96% over 33bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT537330.25025717111171647No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC470460.2191130008025201No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTGGGGGGGGGGG462430.21537309220998466TruSeq Adapter, Index 1 (96% over 33bp)
TTCTGTGGCGTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT459350.2139386067224368Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTAGGGGG433100.20171287813538125TruSeq Adapter, Index 1 (97% over 37bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC365020.1700051599560768No Hit
CCAACTCCGACAGCATAGGTGTGCGATGAGATCGGAAGAGCACACGTCTG362560.16885943453420418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT359900.16762056070404918Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAACTCCGACAGCATAGGTGTGCGATGTAGATCGGAAGAGCACACGTCT352230.16404831924642194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCGCGAATCCGGGGTGCTGTAGTGCAGATCGGAAGAGCACACGTCTG317810.14801747818103328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTCGGTTCATCACTGAGGAGAGCGGCTAGATCGGAAGAGCACACGTCT303420.14131545020511976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT292180.13608050965965296No Hit
TCTGATCTCAAATGAAGTAAGGCGACCCGCTGAATTTAAGATCGGAAGAG286840.1335934471585148No Hit
TGAAAATCTTGGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC255670.11907626772771397Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC250020.11644482519373822Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCAT244640.11393913301094359TruSeq Adapter, Index 1 (97% over 37bp)
GTTACCGTGACCGATCCCGTAGATCGGAAGAGCACACGTCTGAACTCCAG240230.11188521060831827Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTAGGGGGGGGGT231630.10787982905217815TruSeq Adapter, Index 1 (96% over 33bp)
CGAGTAGCGATTAGTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAG226480.1054812575389082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC215850.10053041963870248No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)