FastQCFastQC Report
Wed 30 Apr 2025
1B3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45754
Total Bases6.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC76

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3034466.31988460025353Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT488010.665734143462867Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA10792.358263758359925Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA3290.719062814180181Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC2280.4983170870306421Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT1820.3977794291209512Illumina RNA PCR Primer (100% over 44bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1620.3540674039428247Illumina RNA PCR Primer (100% over 37bp)
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA1400.3059841762468855Illumina RNA PCR Primer (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG870.1901473095248503No Hit
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT580.12676487301656686Illumina RNA PCR Primer (100% over 35bp)
TATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA550.12020806923984788Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT550.12020806923984788Illumina RNA PCR Primer (100% over 37bp)
GATTGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA540.11802246798094154Illumina RNA PCR Primer (100% over 45bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT540.11802246798094154Illumina RNA PCR Primer (100% over 44bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCNCCGTA520.1136512654631289Illumina RNA PCR Primer (97% over 45bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT520.1136512654631289No Hit
NATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA490.10709446168640994Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT490.10709446168640994Illumina RNA PCR Primer (97% over 35bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTNGTCGCCGTA460.10053765790969096Illumina RNA PCR Primer (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)