FastQCFastQC Report
Wed 30 Apr 2025
1B3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45754
Total Bases6.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC72

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATG1223326.736460200201073TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTG757216.549372732438695TruSeq Adapter, Index 7 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGGG19924.353717707741399TruSeq Adapter, Index 7 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGGGG16993.7133365388818467TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTAGG14713.2150194518512047TruSeq Adapter, Index 7 (97% over 47bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC10782.3560781571010185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGCCGTC10162.220570879048826TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGGGG9592.095991607291166TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTGTG7371.6107881278139617TruSeq Adapter, Index 7 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGGTG5341.1671110722559777TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATG5121.1190278445600383TruSeq Adapter, Index 7 (97% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGCCGTC4230.9245093325173755TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTGGG3880.8480132884556543TruSeq Adapter, Index 7 (97% over 46bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA3170.692835599073305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGAGG2920.638195567600647TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGTCGTC2870.6272675613061153TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGGATG2810.6141539537526773TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGTCGTC2730.5966691436814268TruSeq Adapter, Index 7 (97% over 42bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT2240.4895746819950168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATT2170.4742754731826725TruSeq Adapter, Index 7 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTACG2150.46990427066485985TruSeq Adapter, Index 7 (97% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTT1700.3715522140140753TruSeq Adapter, Index 7 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGGTG1640.35843860646063735TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGTCGTT1620.3540674039428247TruSeq Adapter, Index 7 (97% over 42bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1540.3365825938715741TruSeq Adapter, Index 7 (100% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGCCGTT1440.3147265812825108TruSeq Adapter, Index 7 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGCCGTT1260.27538575862219694TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTAGG1140.24915854351532105TruSeq Adapter, Index 7 (97% over 47bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGGCGTC1110.24260173973860208TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGGCGTC1090.2382305372207894TruSeq Adapter, Index 7 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGTCGTT860.18796170826594397TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGTGGCGTT840.18359050574813132TruSeq Adapter, Index 7 (97% over 42bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTAT670.1464352843467238TruSeq Adapter, Index 7 (97% over 48bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTAGGCCGTC620.13550727805219215TruSeq Adapter, Index 7 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGCG600.1311360755343795TruSeq Adapter, Index 7 (97% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGCCGTC600.1311360755343795TruSeq Adapter, Index 7 (98% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTGT590.12895047427547318TruSeq Adapter, Index 7 (97% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTAGTATGCCATC570.12457927175766052TruSeq Adapter, Index 7 (97% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGAGG500.10928006294531627TruSeq Adapter, Index 7 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGGGGGGGGGG500.10928006294531627TruSeq Adapter, Index 7 (97% over 41bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC480.10490886042750361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)