FastQCFastQC Report
Wed 30 Apr 2025
1A7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11922215
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA248522420.845321108535618Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9172447.693570364231814Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1223271.0260425600444214No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1142030.9579008598653858No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG790290.6628717901832839Illumina RNA PCR Primer (100% over 37bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC561600.4710534074414864Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC528380.44318945766369755No Hit
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA470070.3942807607478979Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT439740.368840857172933Illumina RNA PCR Primer (100% over 50bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT387900.3253590041783343No Hit
AATTCACTAATCGCTACTCGGATCGTCGGACTGTAGAACTCTGAACGTGT380800.3194037349603241Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT361290.3030393261654818Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA343020.2877149925580104No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC290780.24389763143845333Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGGATCGTCGGACTGTAGA255940.21467487375458333No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG241770.20278949842793473No Hit
ACTCACTCACGCCACAGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA230960.19372239135093605Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC225400.18905882841401536No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG223200.18721353372674457No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC196820.16508677288574314No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA194240.16292274547976196Illumina DpnII expression Sequencing Primer (100% over 25bp)
TAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC191670.1607671057769047No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC187300.157101679511735No Hit
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGAGATCGTCGGACTGTAG186380.15633001082433087No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA179800.15081090216876647No Hit
CGGGAACAAGCCGAAACATTGGATCGTCGGACTGTAGAACTCTGAACGTG175660.1473383930754478Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC175250.14699449724736555Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT165750.13902617927960534Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT160460.1345890843270315No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT157250.13189663162424095Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG154410.12951452393703689No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC149590.12547165103128907No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG147170.1234418268752912Illumina Small RNA Adapter 1 (100% over 26bp)
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA144080.12085002660998817Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC139170.11673166437612476Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACATCGCACACCTATGCTGTCGGAGTTGGGATCGTCGGACTGTAGAACTC136180.11422374114206128Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA134910.11315850284531859No Hit
AGCAGACAACTACTCACGCTTAAGCACACAGCTTGCGGATCGTCGGACTG132290.11096092462684157No Hit
AATTCACTAAGCGCTACTCGGATCGTCGGACTGTAGAACTCTGAACGTGT130600.10954340279889267Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT129530.10864591856462914No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA127060.10657415589301149Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCATGCACTACCAATGGATAGAATTCTACCAAATGAGAGGTATTGATC124660.10456110714326156No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC123480.1035713581746345No Hit
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA119690.10039241869065438Illumina DpnII expression Sequencing Primer (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)