FastQCFastQC Report
Wed 30 Apr 2025
1A7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11922215
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC239872120.119759625203873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGG8783997.367750036381662TruSeq Adapter, Index 1 (97% over 45bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1209721.014677222311458No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1134970.9519791414598713No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT766960.6433032787950896TruSeq Adapter, Index 1 (100% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT540220.45312049816246397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT523240.43887817825798314No Hit
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC450270.3776731085624609Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGAGTAGCGATTAGTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAG368490.30907847241473163Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGG367020.3078454800555098TruSeq Adapter, Index 1 (97% over 41bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC357800.30011201777522045No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC353310.2963459390725633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG314690.2639526296078371No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT281850.23640741254875877Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACAC252200.21153787278622305No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC236690.19852854524096403No Hit
TTCTGTGGCGTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT223040.18707933047676123Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT220790.18519209727387065No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA220490.18494046618015192No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT193020.16189944569863904No Hit
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC186670.15657325421492568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT186620.15653131569930587No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT186270.15623774608996735No Hit
TCTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACA183690.15407371868398617No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC182320.1529246033560039No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG176550.14808489865348007No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT172290.14451173712267393Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAATGTTTCGGCTTGTTCCCGAGATCGGAAGAGCACACGTCTGAACTCCA165320.13866550804527517Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC155080.13007650004634208Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG154060.12922095432769834Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA153930.1291119141870869No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC150590.1263104213436849No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC140910.11819112471969345Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC139220.11677360289174452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAAAC138130.1158593432512331Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CCAACTCCGACAGCATAGGTGTGCGATGTAGATCGGAAGAGCACACGTCT136390.1143998829076644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT134630.11292364715784776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC132420.11106996476745302No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC128950.10815943178343956No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT128770.10800845312720832No Hit
CGAGTAGCGCTTAGTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAG126980.10650705426801983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC121240.10169251267486787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA120730.10126473981554601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)