FastQCFastQC Report
Thu 20 Feb 2025
POR-69-TP4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-69-TP4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398308
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1559830.8965412038917807No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1221230.7019245779532125No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG846980.48681745374320307No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG839250.4823744929679369No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT825530.47448866866824063No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT787450.45260148285683877No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG647670.3722603370396707No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC636400.36578269565063454No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC619430.35602887361230756No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA581250.33408421094740937No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT525980.3023167540199886No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG489460.28132620712312945No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG451960.2597723870620063No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG436320.2507830071751805No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC426810.24531695840767964No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA388920.22353897861792077No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG383160.22022831185653227No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA378660.21764185344919748No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT371240.2133770709197699No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC371170.21333683712232246No Hit
TCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATG358820.2062384457155259No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC351140.2018242233670079No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC342390.1967949986860791No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA341880.19650186673324785No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA333270.19155310964721398No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT332970.191380679086725No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC330550.18998973923211382No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG325830.18727683174708715No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG320310.1841041094340898No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT312620.17968413940022215No Hit
CAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGC304240.1748675790772298No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA300500.17271794475646712No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA294550.16929807197343558No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA293900.16892447242570946No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA289210.16622880799673165No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA286410.16461945609883444No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG284590.16357337736520125No Hit
GTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAA279420.1606018240394411No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT277600.1595557453058079No Hit
CAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGG276450.1588947614906001No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG275040.15808433785630188No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA264420.1519802960149918No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC255480.14684186531242002No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC248980.1431058698351587No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC248070.14258283046834208No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT247910.14249086750274798No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA247900.14248511981739834No Hit
CTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG246510.14168619155379936No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT244440.14049642068642537No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA242760.13953080954768707No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG239790.1378237469988461No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG234610.13484644598773626No Hit
GTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTAT232030.133363543167531No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT231430.13301868204655304No Hit
CCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAT228970.13160475145054334No Hit
TGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATT227860.1309667583767341No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA225700.12972525834121343No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT225330.129512593983277No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG217970.12528229756594722No Hit
GTGAAATTCTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCA215420.12381663780179084No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA214670.12338556140056839No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAA213740.12285102666305253No Hit
TTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAAC213410.12266135304651463No Hit
GTTTATTTGATTGTACCATTACTACTTGGATAACCGTAGTAATTCTAGAG210730.12112097337281304No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG208790.12000592241498426No Hit
AATGGACTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGG208020.11956335064306253No Hit
GGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTC207730.11939666776792318No Hit
CAAATTTCTGCCCTATCAACTGTCGATGGTAAGGTAGTGGCTTACCATGG207150.11906330201764447No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA206820.11887362840110659No Hit
TAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACT206390.11862647793107238No Hit
TTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAAT206270.11855750570687679No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT204550.11756890382673994No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG201520.11582735516580119No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT192890.11086710270906802No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAC192810.11082112122627095No Hit
CTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGA190650.10957962119075026No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG189250.10877494524180167No Hit
CCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATT187470.10775185724956701No Hit
CTCAAAGGTTCCGGGAAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTAC187440.10773461419351814No Hit
ATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTA187090.10753344520628097No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC186920.10743573455533721No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA185990.10690119981782137No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT185880.10683797527897541No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG185040.10635516970960626No Hit
CCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAA183880.10568843820904883No Hit
TAACAATACAGGGCTTTTACAAGTCTTGTAATTGGAATGAGTACAACTTA182350.104809042350555No Hit
TCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAG179110.10294679229727398No Hit
CGAATGGACTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGC178050.10233753765021289No Hit
CTCACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTT177880.10223982699926913No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC174660.10038907231668735No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTGTGTGTGCTCTTAACTGAGTGTGC174540.10032010009249175No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)