FastQCFastQC Report
Fri 21 Feb 2025
ACR-244-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameACR-244-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449707
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGA811070.4170088526269316No Hit
AATCCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCA545680.28055949634614036No Hit
TCTAATCCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCG525270.27006576500098434No Hit
ATCCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAG517420.2660297144836166No Hit
AATCCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCG356890.18349376676985416No Hit
ATCCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGG331790.170588688045532No Hit
TCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGT315950.16244460649201553No Hit
CTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGAC301520.15502547159193708No Hit
AATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCA216910.11152353092002878No Hit
ATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAG198090.10184729260960075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)