FastQCFastQC Report
Fri 21 Feb 2025
ACR-244-TP1_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameACR-244-TP1_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449707
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAA622250.31992769865376375No Hit
AATATTGGTCAATGGGAGAGATCCTGAACCAGCCAAGCCGCGTGAGGGAA581150.29879627492588967No Hit
GGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTG545360.2803949694460693No Hit
GGAATATTGGTCAATGGGAGAGATCCTGAACCAGCCAAGCCGCGTGAGGG465360.23926324442830937No Hit
GGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGT350990.18046030204979438No Hit
AGGAATATTGGTCAATGGGAGAGATCCTGAACCAGCCAAGCCGCGTGAGG338720.17415172372519547No Hit
AATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGTGAA289500.14884542990801866No Hit
GGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGTG243350.12511756603839844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)