FastQCFastQC Report
Fri 21 Feb 2025
ACR-186-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameACR-186-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16543141
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCTTTAAACTCG757360.45780907023642003No Hit
GATTAAACTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCTTTAAACTC410200.2479577487733436No Hit
ATTATGAAGAGCTGATGCTCGCCCTCCCGTCCCAGGAGGATGGCCTGGCT381500.23060916908101067No Hit
GAAGACCCCCGAAGAGCATCACCCCAACCCCAAAGCAACGCCGTTGCCTG358040.216428065262818No Hit
TTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGC236530.1429776848302266No Hit
CTGGACGGGAAGTTCGAAAGAGCGAAGAAGAGGCTTTAAACTCGTTTTTC189870.11477264202729096No Hit
GGTGAGTCCATTTTCCGAGCTGTTGAAAATTGATGTCTGGTAGAAAAGTA168380.10178236406254411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)