FastQCFastQC Report
Mon 10 Jun 2024
119.flexbar_trim.R_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename119.flexbar_trim.R_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20841898
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length18-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2071280.9938058424429482No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC515050.2471224069899968No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA425330.20407450415504386No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT417370.20025527425573236No Hit
GGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGACTTCA287530.13795768504384776No Hit
AAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGACTT253000.12139009604595513No Hit
CTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAAAATTC220300.10570054608270321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)