Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-53_preproc.map (3496970 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 6427.27167031914 12584.0499271148 9079.67626886733 12111.7003970945 9635.68398647988 11071.5027817596 15495.9748339401 17463.2750574299 29852.1982298458 543838.181005462 28017.1025351073 17646.1138186121 23512.2866872147 15749.0112073044 33088.4489929754 279472.059908756 14015.3776851126 47474.04169372 31420.1185820826 26371.6864936143 30915.6271904 8984.92349120567 8417.05291562117 10037.0055004437 2697.22349865226 6079.71085990838 6123.23975504092 2294.4558902426 152.306689901604 0 8.67380699143166 30519 195111 2 4746.17354070375 12152.4481271176 8874.58997918312 12434.8500935725 11316.434195795 11922.6295485622 14148.852678449 20528.649574355 23075.855771908 587576.670972081 25931.5037814976 16567.4185275923 30030.9197919645 14964.0465820307 30031.9363482515 276685.716285119 14444.0481271129 45772.2343794975 31246.969000171 21723.6742242401 31560.9848022528 10208.6867750895 8377.13430712797 7836.14435963417 2948.80956249899 7577.97145641298 6256.07021899152 1753.05781780782 131.086552904837 0 9.5227048105326 30263 180901 3 5171.48717650765 12908.3521829282 8435.21333055992 13752.832515593 10407.7731677547 10835.7514967212 13345.6665897924 16839.0604443383 25452.6723033311 498414.406554889 23387.3441606734 15200.0193892081 30919.8797939411 13627.1402789036 24962.0053360191 277326.403472754 14609.7171819737 45192.7954697321 35880.0112935818 28155.4457843194 31979.9211500918 8731.56181093527 9102.67310690071 8962.50554612056 3376.49967216334 7050.74611686126 5759.02489682642 1978.8647366408 206.247102159034 0 7.96228548399858 30493 187218 4 4964.80546392996 11565.2028168413 8093.90071215205 11344.0510405161 10258.915679186 12218.9446990179 14852.6156806818 16970.4431990054 24910.672562196 499712.954947481 28562.6628035956 16857.8009181235 30969.0098918046 15173.3929343481 31613.9479653506 305047.021287359 13810.6096333767 42679.129281689 34337.754358104 21561.8837500714 30770.4546344578 8588.68958145958 10913.501214524 8660.52815824509 3159.93841928718 5618.56183636137 6700.77934385517 1616.20559976627 142.54402836244 0 7.20984489262894 29700 179973 5 4946.12168490185 12816.8594582035 7445.78738415234 12685.5779320962 11240.1436347559 11778.3567014085 14538.8620930232 20919.936956313 38491.0804559957 562201.436612078 31432.9597832033 16910.0375502495 27348.749094626 16805.3596712051 31133.823210081 268929.146020837 12168.1659046783 47727.7714899411 39788.4119927543 27441.5177778658 36009.5033642653 9336.61096114063 7600.98041419993 7701.45020592064 3336.9242032603 9190.37923042944 8038.49708398314 1422.81834901761 162.687056911875 0 9.34400399026249 30007 184575 6 4295.05358981672 11124.6386000376 6098.74679729771 11170.3137344813 10766.5684518063 10482.1980775626 13351.0873507564 15661.438660539 30061.426159301 569901.517345088 26606.3811727389 17533.7553045498 24010.264971462 14266.0300192918 29843.5082355726 313706.27845629 16791.2722007258 50824.2138321197 26141.9028073043 25404.1313801225 32515.5644957331 8625.21319176479 7571.82540061564 7900.40109801371 3084.81299815572 9303.58510686495 8682.65022432239 1543.29876124973 153.205885473947 0 8.03336634440583 29989 187448 7 4687.73671566507 11890.8808929295 8319.07243553316 11646.5636632821 10289.9086263321 11366.6973915839 14071.8833018077 14928.0846855428 25925.5753004936 568770.31917149 26406.5599202749 18245.7649918645 26741.8794264164 16510.8093047015 26643.3648081393 291435.547678249 14443.7087150851 48947.9570845494 32643.4578266505 22478.5316497337 33382.8196437694 7890.86969112515 6818.27129929535 8814.03951486189 3305.13373343362 9656.73981391575 7844.26044860814 1359.90531575852 121.920987318712 0 8.68611746079204 29975 184752 8 5485.84420326781 13207.6307151445 10101.2905751559 13935.7685174942 10258.4314704257 12738.1845605882 13728.5551547342 18194.2056013344 29901.0260559672 574110.158118535 27700.537813702 19456.5342130843 28367.2612965368 14159.5747154979 27064.7291619497 291623.170367841 13450.8518825759 49747.5204408607 34090.7035674807 24310.6785368755 32298.5725886625 9131.14751501128 8435.66902406611 8923.60417240777 3203.38519949043 8019.60289134706 6213.31993255176 2073.71071077354 158.742587451674 0 8.77845760351237 30066 178516 9 4322.97613221357 11528.5898084198 7821.68414138455 11428.1183632666 12034.2490332466 12639.8927289304 16068.4285041218 20043.6951270352 26020.9583360906 568292.325691151 29012.6218378324 18744.1995289457 31775.7934066011 16129.6829015468 25056.7348795925 298102.037689826 18675.3678349364 48922.4025583258 30773.8597520427 23162.2419458681 33608.4326369654 9387.04245394942 10513.3570965607 9104.37001208544 3070.77957815026 6551.87561487925 8363.18369990308 2688.05151306574 136.474783321908 0 8.48574855534777 30029 187685 10 4557.89683990275 11585.4041101475 8648.32952424122 11574.7604906446 9870.38631953266 11848.6322728267 13726.6809813118 16805.2710858969 23959.9776910348 492432.962117181 23490.9312862732 18900.9804513782 30131.8075083154 14277.2111399139 28286.340825691 299976.52558592 19171.2195309182 44919.8694097001 37106.3816648041 25250.3877982169 36466.036562746 9348.10371133606 8479.24454241806 7837.87403160701 3341.00767906452 8236.24989361685 9183.87782371764 1828.9202577826 138.369487345218 0 7.22579066086302 29161 187178 11 5397.57636062894 10602.5012601817 9054.64286619042 14756.2545835035 10543.965331142 13056.7874600037 14611.6172064075 20817.3365685973 24452.7523123531 554215.580341943 24924.537771461 16344.0880424268 27679.4911701303 16857.2206668507 27623.9332976254 301948.673318718 10282.3240511304 49269.1125428452 31818.1947294876 22228.0036033129 38725.8334147443 8482.66819718267 8243.32671257341 10010.4697554096 3375.79962084613 7589.92376640246 10172.3832488995 1305.68444723381 204.448820384287 0 8.1407116730795 30561 193176 12 4404.00952275325 11060.3973848606 9766.39912862976 11713.1028816129 11398.2413069122 11189.7084094873 13052.2461322902 15151.06379333 22224.4908045991 521078.595681921 27821.9623233009 16043.650912123 25197.3129911256 16002.4779726713 21184.598348127 279994.126561108 10693.4572473468 42606.7062189038 24222.8112401325 21316.0472628471 28534.7366130907 8381.86020652263 9775.80120751817 9027.15696707533 2484.83179357871 8450.27833305836 7028.07141470903 2578.78499666131 145.547298480937 0 8.2706049652413 29714 185561 13 4641.38661616662 11838.6325406085 10059.4396864145 11403.5324843291 9899.60005901716 12411.3490421361 14678.3939159143 15924.0809734201 23584.3719953941 475398.578823056 30000.2544773874 18602.3149160385 27856.9104743628 15095.5739931238 27874.9614773327 278138.307508165 15663.926516421 42047.8832748963 29204.1489307446 21362.7135702527 33748.6714153296 9197.76547356958 8455.0880960504 8611.44522591587 3242.91727057698 6044.91943770461 9869.75716622434 1963.91613053319 207.871962393589 0 7.56215227313729 30030 181713 14 5484.67763590888 13158.5345434025 8857.13249257036 11615.8235478992 9164.49199567584 11223.8657249175 13675.2377203178 21598.3931052215 23157.1516024366 534851.533188117 25790.429408164 17368.8075751517 21931.2011208487 14200.6132114649 25690.1272778332 318238.948439449 12416.8859295373 45139.6317424858 33539.3582017611 24702.3886248196 35413.5549342624 7780.1964323529 8891.73483847915 7392.43063643 3766.98668994798 5800.48750493864 6541.13322567049 1090.57338756675 268.336315705608 0 7.40956748741769 29756 189108 15 4796.13100281114 12532.7757486448 9262.40964823443 15276.2425243147 10645.6334689565 11685.9984316329 13961.1761916523 14501.2647572375 27103.9673415576 478772.105954333 27594.5952511175 16678.256442763 27685.926966359 15269.1963926526 26917.0416279398 302015.477717456 13704.0601411214 53879.417114906 29519.0331177568 21086.8773959376 30020.6016305474 9340.1292084989 7999.25003765751 8037.37070872457 2850.10520512737 5420.44993299207 7943.17832679599 1734.31267762418 125.947858440199 0 6.94603214099949 29739 177622 16 5083.68286707269 13792.3781049693 10476.089475807 12442.5511434165 10055.7852222088 11776.5698616426 14372.7451564013 18036.1891241833 24457.9934842723 536996.331003368 27861.1720885638 17603.1680842935 24656.8327503195 16520.6667500318 32204.1239660234 258127.115713703 13317.6473008752 38017.8819727343 35946.719432885 25262.267153326 35180.3473588983 9587.59908976813 7139.94105340022 8259.18859338274 2689.56717173169 5782.66393703394 4334.67943303726 1498.95944086239 132.240078641431 0 9.34574589926006 29852 174682 17 5772.40450090188 11908.8651845907 8527.16863325416 13111.4871020605 10423.6639248114 10427.5784557471 16377.9887229369 18819.533007803 24481.2994186506 566356.326924707 24961.7874600829 16822.3918924895 26423.9484528443 14320.3239202041 23927.5472842841 311455.893458032 14367.2482263119 46244.9439258912 36325.3998651272 21749.8140577956 39093.7345691208 8474.31919773553 9673.41938545788 7152.6828849346 3246.93657558714 7748.41012743348 11116.4833383448 2061.48824191801 128.367407516323 0 8.06064883254953 30102 193348 18 5143.98517951144 12446.0367997995 10009.3809152959 11185.6353227624 9563.8363497058 10202.0195012252 12663.5194286116 16087.1345013949 25161.9308982506 529215.676742026 28147.0957831545 19125.8567828147 28690.8344793237 14820.4238789976 24615.1779234651 287000.674653787 13942.729553616 44707.8295962396 35637.3025140688 24354.5251766826 32430.6127698 9381.34017336352 7923.60906296259 7890.32120131608 4582.49576670456 7643.08694979708 5984.17280878965 1473.68510048068 208.981783097441 0 8.18333966469303 30111 185412 19 4815.0656319987 13318.3930790769 8935.37865582898 11438.4682974721 10075.2082377166 12063.63329711 15224.0959815684 19857.6908092072 32620.7794305417 563748.681679384 27681.8997127929 18206.7855828129 23822.673299486 17165.7568103902 26439.9835464591 285111.431469161 14208.8027131324 44939.3964350511 34218.4959463811 26074.0237546851 28910.8125208138 9237.56244947927 9071.84027467566 8642.19940136998 2911.85602914517 5656.45936707413 6815.30533683704 2083.19727374541 133.392902744449 0 8.82963539470806 30434 186687 20 4857.58955400951 14997.0034776193 7790.83672277942 13601.3997801256 9337.41163539256 12014.2326088469 13083.8501651264 16680.551240965 26507.8456953615 527137.099243815 27619.8815659906 19018.9444621927 25758.5831505155 14747.618660849 25788.6360636666 344866.658477328 13516.7940707104 42734.023733626 29363.8259468557 24388.017903241 34894.1253320428 8300.87030891231 7219.65524480367 8639.73591149452 3114.88572507964 8860.88837366673 6977.72508276552 1448.67569025513 133.374507467221 0 6.69695492658328 30262 189757 21 4812.07922036625 13244.9584983229 9592.89504223421 12374.5894504452 11197.0066297994 11352.17712796 14258.4735849989 16848.0364453814 24328.0947011755 552961.896610187 23464.3867913112 17011.3845232271 23067.4006088048 14897.8962580017 31911.7348479908 277117.064031595 13278.0171000671 49315.5987392084 32267.5069693184 18795.9582700796 34309.3612940607 9545.25135780423 9642.78440662467 8417.44454264412 3557.39979982897 9255.13078048951 6158.28640572456 1898.75420994838 111.618481976061 0 8.89612571593086 30265 185760 22 4875.85179580884 11771.2069207059 9499.41075306593 11646.0028768258 11407.141116089 11626.5474732174 14884.6176519957 16329.2463274696 30565.2484998606 518613.818409837 28589.555569219 17895.8309134941 35376.7594562983 17272.854909066 26571.1829932299 277038.76314002 12646.6837828681 41651.2162032896 41393.5395680247 29025.9482256051 33743.4875493876 8781.01239466192 7267.19861274239 8218.12793742039 3596.80090733885 7807.72523875992 7058.97496736339 1971.16946902456 177.240485371106 0 8.31839399392721 30173 186589 23 4593.20281929579 12877.6041189965 7329.17300722978 11921.3037737357 11379.7910212805 13347.5551968847 14898.3557987266 19939.5513005719 21695.3653755971 578141.574686522 25070.5630123116 18209.1412342122 27992.8073422114 14261.9908810571 24590.9871238317 323042.405615102 15393.4974780507 54719.8421671467 29869.8715730763 23884.3206842276 35331.1566786475 9513.06619466072 8265.95382164172 9213.1952365877 3382.80391460198 10932.1806514672 5078.7543908536 1787.2689049783 124.540478359619 0 7.91035948632493 29867 183983 24 4941.07515969254 11511.4310895538 8077.30115029023 11144.812667316 9975.37869164692 11907.2878916982 14598.2104618539 17201.0328251042 22535.8630274949 538634.280240929 29679.6780872726 17455.9428254425 27106.4442481348 18240.1765159404 24239.5772060194 297089.464222406 14222.7403943341 42621.126367161 32930.917677258 23444.3826638714 33629.5042749231 8133.715101975 9468.2518816869 7941.7298563366 3370.50775964602 6223.29720979192 6332.13269753335 1409.75609683229 193.20990679797 0 8.04463032932752 29976 182759 25 4667.89197442673 11817.7836567624 8664.01062538344 11773.3573198899 9079.47238505111 12531.8789270796 13663.2468721923 19185.5599645917 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10127.1397891423 14498.5641072927 18983.6013422847 22814.7953029008 575037.002974475 25698.5821239947 17901.5123812162 24958.8911958452 16233.6223707299 30561.7593255738 275901.214954382 14950.1629864709 43872.301308324 31487.8770764724 21183.1135141032 32545.9483488169 7904.40555308744 7133.01550936765 8206.68951923548 3169.35477024035 6537.16762093431 9068.17395474791 1804.66934100652 318.615803882876 0 9.33450748711139 29931 175232 28 5442.5398666108 13519.8376721397 9868.82217280567 12819.1778359619 10713.7353030146 11309.635696521 14139.2528928898 17632.3045677022 24270.4884866639 514535.674698371 26781.3615973379 17534.1820331789 27388.3554026429 16383.5474707209 26994.5910276706 299488.812013724 15061.468818864 46035.1694567002 33329.719690194 22748.1215234025 31913.9880204661 8393.28410166365 7356.77759116154 8061.3615642411 3666.66187822021 9807.99986063597 7905.91796158788 1876.40819071941 86.3425560320293 0 7.597406643758 30487 200823 29 4700.4238779423 12890.4412479871 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12090.8838176161 43146.8622384098 28955.7009065752 22208.8959138755 30788.8397627859 9990.09882938883 7492.87503433804 7444.41682287372 3110.64138171235 5695.17017389209 7945.21007841971 2804.75446283561 232.607124461204 0 7.87148005837201 30321 187911 96 4451.03175628194 13520.5537929041 9968.66767294435 12377.9517338462 9490.86622833342 13159.595924444 16070.2051491378 25828.3572641871 23794.6937178037 572799.237132963 28089.8781395664 16953.3025382289 29901.8767247803 15048.2015066824 28595.7922605906 267765.817737409 15311.6061891759 44432.7754379515 29372.0027174515 21127.280366919 30690.2567825686 7700.37820812931 9413.17513398658 9198.96343002406 3088.70650730629 9166.31508781474 6677.18125665149 1204.50079766379 214.924465186654 0 9.61827599448897 29783 183895 97 5131.87677576206 11123.6514313943 7890.42486925937 11543.8904150556 10066.5496130358 10748.4964964729 13474.976231496 14474.7888284888 26339.7198628264 517899.575820455 24445.4424414588 15321.0427827695 22189.6669247744 12522.1635222124 24941.5761022812 306968.706187815 15589.9462245922 43492.8839181771 34902.1053840105 24692.035475736 32759.0666962558 8920.49187688099 7273.25792158109 7164.34711888097 3121.8854518858 6182.13742785144 5539.2216127337 600.619536820579 178.021381242114 0 7.43485739579579 29701 178632 98 4999.16809947078 11862.4587986781 8575.38254713097 10385.5498475632 10356.0715275977 13692.2765208264 14591.1229483412 16871.6155215354 23755.2118089026 536906.483979103 29237.9693852204 18032.2576327202 29431.8856449705 15430.76467434 24533.1154303765 265092.342096716 13097.0290584817 44685.0560661321 31793.1389363067 22774.964590611 30747.8163998829 9337.04152720196 7567.74475817992 8011.01888513463 3255.54719660022 7604.50460337148 8071.76526501707 1367.28132716455 112.338245544474 0 9.05734584225207 29931 192359 99 5150.27720284236 12251.3467924982 8217.22553406694 10790.5359810292 13889.3686732983 11566.3350157625 12801.8406724009 13918.1085835271 24656.9932398526 587530.770471356 25295.2575443275 14209.3393110239 27542.9763839499 14675.6025012682 28943.3380062265 278251.713118772 12909.5179655085 50860.9274208 26007.6942656675 20526.2838314733 31645.9147300592 10352.214389164 7366.13895322207 8329.40022084118 3062.93234353471 6289.01320351023 8133.25514532389 2113.4558085468 154.010140975707 0 9.4332294044334 29667 179316 100 4617.28729292195 13058.6657068294 10184.9340366503 10702.2399973834 11422.3982809075 13276.8581596417 13505.0110168802 16362.1780497952 36844.0674953315 496056.469718183 23815.5934766796 16720.7010319079 25027.0002754507 15101.4613626862 28585.9336247078 312096.735491877 12246.8001131954 39610.1859044513 27046.4685789587 20563.7536749495 30502.0806221557 8685.45390482399 9444.49387564644 7674.16372294617 3631.60101267723 7657.5848442519 6067.00567468221 1048.03259784324 232.605119798845 0 6.98583382532976 30232 198910 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5120.88343632904 12507.8924209876 8974.05133158653 12321.4617007787 10375.8239007887 11880.2313507967 14398.1645896683 17599.3570675834 26685.9122369721 545780.511191897 26461.4093623991 17032.0460308288 27088.6514379779 15263.6166136638 28118.7364347235 297828.469256845 14150.1257037479 46303.0635114832 31884.6387719469 23027.9700313209 32989.4368390573 8814.01854300271 8235.55774072515 8155.78202744655 3165.34208138134 7765.99515365956 7157.34874071659 1694.80663364495 154.074387761053 0 8.15599629920941 29944.37 184529.62 DEV 538.361401882498 1335.3217536811 1067.81384927075 1137.97763407943 931.446797188986 907.454540421925 1023.65197419464 2531.18757051513 3910.58128931173 31768.3782341935 2019.68459141751 1130.03350986574 3036.30888135605 1438.02802725598 3147.79564190144 17082.6918919301 1644.63679089647 4089.89087649524 3812.77357265796 2171.60245318195 2511.28871212325 733.708195418166 932.132589765103 967.668959669203 336.881056344937 1536.95149073781 1427.60005210632 442.022824864652 47.8160469591908 0 0.680285034665991 291.847893773452 7070.02910995421 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #