Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-48_preproc.map (1981064 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 5870.90180983316 9454.08473481914 8209.76120840471 19713.7134606962 12959.6732124058 12462.8956516555 67461.3116468218 35670.7657330081 36538.0235280378 911881.726228385 111318.905253213 41226.5102628569 48063.3483482049 22390.3251691237 22750.1052248906 19161.2780814899 19975.725019611 48861.2970780639 12267.089141723 22509.5403214321 33477.3481745471 10194.543651297 5492.34518704628 5515.32880885901 4944.74225890091 7774.93991237182 9789.43966247179 1369.94616679325 232.566283898182 0 35.4049761873048 26152 230887 2 5445.35062343375 9467.0949525345 8492.18515359154 15484.4117379032 14481.946380837 15924.0796226015 39791.5161373189 28132.3757357059 36035.5547826196 922789.712180346 103653.53643595 33959.5775514187 52816.7959064721 23524.4388995608 29677.8330430801 21156.5109795614 24963.1236914362 47234.1523805342 15064.2662597638 27119.081693147 38221.5892759206 10402.0638914423 5483.766880793 6743.82080934046 4251.05514830608 8719.93362020221 11120.9569942478 2154.73941772631 403.899754118639 0 41.3133148345566 25957 232785 3 5078.34328101278 9740.67943457652 8601.35985178286 16531.0725616385 13115.2947802425 12964.7350206086 40991.8809155074 27074.8655388679 66896.2984912507 1009632.66033113 109043.587479593 34874.3013290289 56428.7834208734 19628.1824649725 25643.1838103375 20036.2406855304 21702.3219090505 46798.4758094957 13633.4460844545 22327.0780524841 33393.0496621809 10404.1241584817 5809.9594625957 4069.04133319261 5089.40970733121 8353.01973119352 7241.15469122597 1865.1744424471 341.56642225569 0 39.6267283213253 25771 230501 4 6272.2189271483 10583.3240187813 8074.922945126 15142.5577879984 14844.9849296311 15305.3519302252 66350.5890985027 37372.4954616322 61949.7149703917 1022239.16592789 87123.8800482017 22868.6296144695 57125.4406989424 21084.6676051167 21712.5551236254 20505.1803571424 23370.6277369992 46112.5451722769 14548.4688494584 21702.4477481724 35044.8018357876 11049.6459698727 6057.37503779752 5158.43536116262 4960.05696625133 10013.6684774349 11857.6769036705 1923.79871329481 147.372299766325 0 44.6461374663731 25918 233366 5 6337.15391890977 9877.54541735918 10514.0805776371 16350.0600955733 17119.9353635015 14346.6234094754 124236.805790614 56131.9174003953 118487.143021843 924252.585952142 79837.3533907571 33524.0791041958 53056.7391803783 21821.7423277523 22977.1486318751 18700.2875677805 21982.8993401964 47234.0888174575 15732.7310029865 20948.3412285209 35878.0258513696 9914.70343245548 4942.73219949477 3809.64597953495 4316.28251529964 10175.9279238903 13126.3624694527 1834.28577442364 197.904325432641 0 25.9273978550098 25695 226101 6 4913.04379691055 9883.49383474245 9690.29627800872 17732.6582045139 19752.4578105392 16285.4119068579 94071.0167507234 44932.1991877181 114868.485192943 1078613.38198681 151371.606378271 42601.9864875945 59744.2774429668 22190.5776055491 22746.9366631956 19303.1095230676 23111.4054692864 48590.5979290143 14846.2780599365 25975.5440226362 32020.9200442273 10136.8883810421 4903.28385274226 3871.65328425975 4204.15023636867 7147.45683417676 8612.92987616097 1629.6757042939 190.22431523829 0 26.9988163857722 25761 227870 7 6557.1295549371 10897.5906729083 9572.05320340044 13628.91601946 15460.5748277243 12173.2637259544 37528.8208124005 25659.3156245811 35228.7875623623 915759.71272247 62269.9481394195 25500.2839551665 49171.6197473731 23651.7727620687 24311.6582124888 20051.3254409162 24110.1845423429 43357.2180838281 15341.3582103808 21397.8376957351 28469.6161682373 9380.71016645354 4808.39181760575 5119.25573022499 4262.41299812003 9087.52947179112 8960.79192105877 2444.42898951035 177.164129116228 0 62.4248715738658 26089 220657 8 5680.31678112519 9135.31844137558 8989.88145068161 14998.0903598331 15822.5101644204 13702.2991454455 122499.725944819 53773.9049805572 117983.524924489 1031758.54119498 81988.1141460915 22484.9335103839 49431.7777676105 19308.6714507812 23183.3734313798 18622.5645102668 21540.083042767 49732.1908394038 17049.0038775348 22514.7099730384 34290.1461414644 10272.8150764353 5544.76942331627 3609.66287338656 4666.40193152749 8337.33797113808 9178.27849423015 1494.10967604841 238.87487162707 0 28.9965090076136 25739 225008 9 6864.2750402303 9843.3463041962 9528.48949139212 13221.3059646913 13841.2522871601 17082.0037717946 121548.846274853 53967.3179124925 91882.244100361 908740.524085682 105668.403839692 44890.5539973142 53220.6581218444 22156.196637293 22596.0282427569 21983.9124540761 21753.670239728 47785.5061685448 14432.149856087 23333.8270169939 33022.0306859035 10262.7166689985 5567.26570686764 3306.1583393259 4357.31908485303 6999.71751615105 8939.81837752108 1852.29815652625 183.85469824199 0 26.1529759501241 25501 224189 10 5171.26001427349 10290.6537479572 8953.95925791322 12497.4858152757 14600.670509668 15607.3674371208 40662.3676262306 25237.9128845804 59403.2821668524 962494.210841209 93372.7018657317 32735.4226692912 56352.759552987 21059.1491736243 19083.3250884629 21615.5695781906 22156.0586690577 46542.8161201698 17500.5273533668 21321.3241946845 31055.0647677355 9531.5300220297 6509.64388495687 3623.40436062539 4638.61887847249 11157.985444987 6779.17967005946 2327.33306961777 243.59406063792 0 43.5948646050943 25665 232646 11 5912.07723952604 9506.19919351591 11898.477628816 14802.2838418121 15680.0830446488 13092.8012854118 37473.2563582237 28236.4335892794 117937.415960171 1154267.39389492 173993.092329095 42660.2535247803 56948.6761515335 22753.3553011977 21743.7158998832 18840.307032988 21588.4207392619 46194.6851010392 18469.3333329159 22421.3475443319 41196.8448812251 10290.4291325594 5214.80763046065 3539.60703360552 4415.31600706757 8419.91757498207 9715.35149089317 2485.35662915141 198.010548702332 0 27.5135980735421 25575 226158 12 6356.30724818271 10374.3347713465 8868.92867783011 14593.2255942491 15142.3712932604 17036.8349945082 95777.3519584456 45000.8072869152 65936.638047536 944824.240857155 79873.2536873395 36449.8127412401 61619.827536254 23882.6033166241 26860.2590644838 20525.5280379598 21813.3299845325 44858.7834279237 12641.112553885 21989.7419622977 42565.1311655581 9374.41192157713 6674.69995188039 4953.50265831008 5069.78682540294 9677.05533345678 8682.07847491673 2117.31447033674 265.947575605167 0 36.2889485816308 25819 230197 13 4958.6640420863 9088.31759364381 9215.35863917086 15225.2554966818 15630.6513165961 16130.2795252359 67150.1697354412 34650.3614319632 91321.5002705257 942569.477466463 143158.201871109 52631.5917545073 51741.8265840272 20060.9599951502 21332.1832438341 19577.7212011746 20769.8206999186 45703.444218338 16414.0074116891 21050.4117724072 36312.6013524847 10738.2014074439 5584.23830120219 4057.50583766944 5607.08118951635 11212.8110547682 9750.56648086186 1937.16307968722 133.251141311765 0 27.0541770355446 25574 232976 14 5393.37105590213 10711.5178537711 8722.89026340782 13750.635158654 13545.126254464 14614.6462068499 40490.2335129747 23629.2563234447 142131.748424978 937978.293510847 107406.046415713 47308.8760170411 54769.5942841701 21144.8499055949 22999.4132900862 20219.0666053138 21386.5073330998 49123.865503108 15871.3480159205 18227.0522766455 31480.1492815497 9399.79352655299 5177.14694877 5540.97354186017 5028.02041031419 9067.10673087774 7803.22297228023 2363.78911809429 127.888200376126 0 26.270689358247 25922 237643 15 5576.06542777487 13168.0903245556 7163.20964203796 14716.9679630995 14085.3873104175 17149.5143793036 41144.06353985 26507.1570114018 67864.763538336 922545.213830546 93225.7514648912 30937.0418429397 52922.9749155214 23054.1055113468 28090.3188128333 17598.4339540034 22416.231123272 43204.2317743777 13640.6397780876 19635.2520193373 32033.5271926314 10790.4490896357 6494.96750546555 3940.06752441986 4428.60377401998 7748.46980470946 9592.48938844628 2144.65070380785 296.709485348256 0 41.1833958772019 26537 245085 16 5989.25792533842 7212.21201489936 10016.0704591034 16930.5256325276 14851.3928522226 14717.9375484076 94456.221684859 41789.8572501046 34074.7069793394 998111.430743058 97860.1297056544 37388.7916397575 52478.1263561917 20899.9769495424 20786.324267528 19841.8736312615 22703.8012988507 40050.5963548734 16484.685763127 22771.8664035932 39972.303342995 9957.07355120004 5023.80675027985 4696.88733924374 3861.71432825557 6558.35547575011 7269.82195007446 1557.33488629774 288.556605816605 0 38.0981875420039 25693 217015 17 6145.0453278254 8454.42506988006 6907.41409729033 17155.0588252975 15220.9607803537 15038.1828971019 90815.9197213383 44510.306626322 42056.2554753016 973585.753302678 70333.1204589257 25453.4476656481 48556.1305585386 18967.0855250972 24564.9730564353 19117.4298404461 22086.879017601 45737.0249971979 24100.6702882358 22243.7882806562 31917.194865514 9801.52227715601 4956.2406835539 5939.12104396399 4361.03043351503 9371.24722248807 7144.43464110881 1346.22309918775 227.370164962894 0 43.6169725139263 25628 223503 18 5986.25697961602 12915.5151144223 10241.5646836004 12499.0281008763 15182.5941848149 15155.558133527 35837.8797332986 23729.9681145524 37416.1222294527 960063.175998228 101619.986803023 34689.8139294845 52523.9964423897 21109.2567828984 21804.3685981019 17669.4224306193 21070.5430144841 40401.0997369673 15626.8232986756 23255.9683443033 33656.8981135508 11276.8549066818 6083.49717111855 4419.30373094003 4839.81779091756 9012.10299272981 8630.18567637078 1626.45597404943 152.257742369634 0 44.3822572585113 25799 219826 19 6835.21313636572 11107.7778594215 8396.61074865291 14906.8451513012 15306.9466753702 12487.6509309288 95075.2146323977 41454.9279002279 91743.8725544819 969485.97673577 79721.0652786469 36277.2878833533 55284.0593335138 25077.5378421112 23542.008214507 19668.197926667 22263.4621878305 49628.6829202247 15813.5634740459 24309.3064222332 35777.0572113475 9540.91869976961 5376.1431438258 4627.45983172772 4229.20393023497 9323.84590591323 10019.8682683988 2106.27010032128 226.431094265115 0 34.5020260352599 25799 238863 20 5640.86011785396 10724.6981458075 9759.79143231728 15569.2929098805 12034.1734022624 15551.665329495 66079.8978741325 36513.9415070345 173707.687879935 986403.49770277 126862.544870126 45829.5947841629 57354.0529701812 22702.7228632724 25496.7107788151 19490.2712247237 21642.6210757267 45922.3557825548 15909.7284172575 19124.6374834031 34395.5315914977 8021.62738812675 5495.72683171851 5789.65968665227 4523.86992693462 7894.0788220243 7490.62002801258 1867.08266668147 207.774455789831 0 22.4937611023597 26124 229377 21 5137.2099972291 11406.1282656858 8720.30420741931 15613.7322129162 12644.7304027878 14434.9410127619 92351.6519274949 43846.7412616898 64852.888629844 994906.904214814 114914.665439156 34699.8169557303 52323.2830728901 25675.0159655647 23397.4389215242 20009.888218164 22006.2454677599 45779.8429575294 17275.5695254963 23353.3165288897 35370.7819737184 10034.2367380831 5622.57413572302 5025.62635390003 4865.73208745143 11052.4058649889 7812.36920814726 1843.48716467369 190.968375772231 0 33.5847313725595 25814 229545 22 6408.28566581 10736.7998907088 8211.80877200159 18448.7902458548 14020.9786141658 16974.1749050698 40615.4786483704 24664.048322424 67889.8232553669 1064994.14906052 97448.4676276078 26670.76171728 60259.5964921923 21766.5634398912 21669.8043487666 19560.6609893504 24783.1464509151 48188.9076951559 15074.87062973 21803.1103769176 35594.3507047565 10384.0254304517 5325.14434175454 3549.0641245334 4335.37585365526 9050.2257730459 11252.4846584261 1938.23672611962 128.014122773801 0 45.3376702916074 25883 220864 23 5673.76373494246 9859.74699840093 12100.1484898126 15897.7431941624 15149.1186473808 13877.5036093363 97255.174342093 44779.8895219599 89857.4626362879 1032483.70136964 86230.7255291332 25514.0509455963 59338.8765517398 22425.8115207635 23089.6389946863 19015.9531348505 22340.4529429424 53910.6931328286 16177.4920456682 26577.2489495392 36126.6554264086 8284.36005608924 5566.85609537178 5688.48621067529 4624.62255327536 8840.00757164529 9060.71469888018 2167.4186406594 234.213506908405 0 35.50478383936 25954 238618 24 5251.06950220515 7867.4349145769 10688.4229036604 16064.6627448254 14569.540427647 14398.7780150799 89873.5241249533 41481.1430948457 91556.8074006311 987247.965701622 72513.3040446344 25983.1024820558 54885.099686134 21023.9218623001 25014.1883797574 20442.9607412906 23103.2711278296 42015.1545035543 13314.298248948 24006.4384474766 32880.5691329794 9475.63701003415 4968.71881852375 3613.92799414951 3890.39037165905 9180.33572870127 7940.30760301986 1490.30872059962 136.408518709163 0 36.934877992912 25627 223874 25 5063.06803649022 9316.68482528465 9352.52245749484 14031.4670340205 15048.5388645105 13935.7497747885 98694.2977492384 45135.6979138652 63331.2029987496 941370.01676104 64893.4937697534 25998.6139885916 54122.7149046231 20610.7022920816 22604.0045015763 20902.297126032 23325.8586667446 42783.7592973329 14684.9460590317 22621.9914025685 31557.5925145421 9685.49736202452 5067.46162326176 6359.10879947696 4538.54412172012 8829.50408002207 7536.98563659377 1994.65919052004 237.309141948387 0 38.1979935541873 25545 222370 26 6274.49414159792 11368.2524932236 11736.3640613453 16024.7224673574 18651.4386010555 14692.2850512613 40497.9909058605 24726.8055527564 63090.5483023343 943877.780837129 67056.2577355319 24023.4980869542 51885.4479192198 19493.8405825782 22163.3545309341 21123.68156219 19829.8520326211 50506.0185125472 17425.1979115869 20259.2833557052 34189.2978817225 10686.4079726841 5657.94842617878 3699.55104975577 4551.2494902229 6846.34574090868 7412.41106895622 1830.75886628607 247.94146427454 0 52.3496804145686 25399 219710 27 5793.63452025771 9982.95171951788 9338.91989242434 20254.2530222601 13561.7315702571 13396.7567428637 37252.8246135004 24978.4736732587 66564.5549947996 995683.939123315 69278.4769669711 28348.1995151726 49293.0023032458 22764.9243987542 19078.7293429019 19727.7539024381 21962.1059606199 51330.8220884165 15493.4349469163 22465.8762529413 33866.598001046 10093.0350466381 5184.85853405072 3633.7618741607 4394.41176432865 8017.04613719361 5408.43934008521 2494.87021011283 189.835293125716 0 53.0742470427199 25746 228782 28 6326.04698835627 7254.58146870636 9185.88799123492 16628.8787726085 16219.5701937859 14669.2975442759 122244.304936786 55645.513470244 66006.5933730634 848938.056248867 37009.7047617458 23428.134398034 56765.8545832279 22380.9724393416 23315.0686804254 18819.3596504875 19666.756025413 43936.5167790822 12781.98473724 20567.887852766 33790.1906723956 10429.389572878 5470.15082403344 3528.71196092577 4496.20752825455 6922.5488422139 7552.5710241741 1817.22279937926 335.753369993306 0 29.8350313722807 26127 199696 29 5148.22363834937 10413.8468585794 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5230.92675836267 5142.09960153215 11757.2777879056 8955.38158702429 2091.36889850641 325.815345864852 0 29.914340939121 25936 227120 92 6294.4587108304 10411.0157856253 9949.94660437257 14109.7473790932 15296.385546912 15589.5001815657 67102.8815067744 36613.504237212 94526.4343516432 995299.993429172 70607.7586512592 26662.678335701 51102.5703388483 24734.0743885115 20206.3433773114 20128.2013830546 20983.5724217529 47129.3648555032 14701.3586216611 21340.0257667541 37815.0322040702 10747.3567551066 5902.30702109244 4744.62564520219 5135.90526702619 10330.466125947 9717.00677383162 1606.56299558424 215.735137192564 0 40.824924827763 26111 229521 93 6306.38942474573 9813.88651508107 8144.98828181552 17143.4050422973 14800.945432816 13248.2720154051 68458.6686125511 33550.145697874 86771.7737462079 1098252.09560063 106433.759393084 23600.8533107194 50075.6190446789 22620.6237599059 23208.3603959457 21158.4150653355 22218.458080417 51438.3850220949 16714.5364434033 21167.8363238709 35698.0102545684 8056.89369498596 5401.84909557184 5143.99061296697 5223.41849475144 12748.8230512228 8016.9786370611 1663.92588257346 201.983413648757 0 39.3162756665147 26130 238184 94 5505.71507781817 8404.79567978513 8518.18331249844 15346.4365515798 13830.7466270737 11367.254239313 63023.3315530625 34519.918254976 61566.1385481 998566.061863022 124326.177402094 43098.1143317282 54884.5970601826 23800.6032246016 25000.9021517295 20501.0621981221 21023.9555151552 46396.6935771088 15291.2025513948 20022.770832493 33449.1063639445 10085.1673271819 6224.9487627554 3681.5916160734 4773.10958402396 9743.68075389761 6834.67060886036 2007.56435503473 236.35405061781 0 34.5551209626041 25448 215992 95 5895.43808883171 7693.71971017443 8806.05399886732 10319.5834944644 13771.9694383019 15194.3622412857 64981.0304408417 33244.1058608687 37581.5345259698 955816.502047063 70005.6939624886 24068.9892062129 47399.7371645525 21088.2577728873 17869.5809134512 19441.4521969309 23032.1786381 44926.0164394312 16994.4352322131 20318.4153902852 33225.6203630031 9430.46099938937 6005.90819548034 7023.46762570608 3914.44011000393 11222.2338448377 11608.6733417139 1202.9412683067 247.377425603526 0 51.1959716067887 25724 213774 96 6284.84062828619 9318.37756412978 7485.16517589321 13986.8432635766 15512.3126562196 11652.7061699812 41091.1175774327 27888.4413965635 36812.1179921463 969031.668092704 103383.664965495 34734.6049244302 54845.1280973456 24445.1961332193 20086.529857875 20311.5963038328 23172.4553793856 46237.8221214407 16105.1940831772 22997.8888866217 34771.6844324848 8931.48886890929 5530.27042746432 3743.46836824902 4650.37705982832 9430.81040519325 7775.7713766958 2562.02911069387 95.0169369101664 0 42.72076872099 26115 228739 97 6374.6367530772 9395.1834493029 7294.27857955197 13707.1515293543 13685.7752534685 13428.0422177176 38528.5534649794 26859.4420406689 142773.594537924 944068.737537775 120351.338735886 42766.6169914278 47571.5020991166 20153.8481249415 20889.9000689508 17865.2728127663 22441.4201058975 43206.9927254671 14302.0276142606 21270.367861934 30045.7343466867 9318.66036503777 5058.59709443444 5049.93645221368 4629.24215022017 7666.65491883228 12482.0040745621 1460.85676017053 167.129700032237 0 25.4302868614461 25494 216486 98 4333.55738401081 8489.8482061972 10713.3835374925 15450.1874318596 13694.6625832649 15225.5075656128 40443.6859406245 26667.3242546221 38909.7669283202 941319.591274407 54406.5316606172 27163.0812494176 54897.7232661259 21027.7417504194 23247.3459260463 18761.219557862 21345.8944596329 46894.8256448575 18487.200865456 22299.8851240283 35936.7620484538 8955.47446361394 5699.8768809458 3654.51362963541 4661.22562400026 8196.4558061347 9108.90901693111 2374.41922657131 282.572120232953 0 63.0181004817134 25633 217920 99 7824.42464773853 7705.30393652079 8295.26239460297 17814.3107471567 14118.6547055152 13168.6254155614 41677.2456916066 24377.3074941918 92902.5751621165 983674.621250884 99544.8079999296 32532.039079579 53159.4113923606 23840.1074682989 23623.8216284333 24174.6647096645 21255.527666769 44980.0694550576 17280.3379797434 23319.0044429005 33804.8605221317 8545.98953859465 5375.7965262079 3609.00264607372 4506.33123253448 11374.5095725455 6639.89109816686 1836.91362123654 190.876368643937 0 37.5242947520228 25622 220439 100 4973.98667642833 9224.84959694238 8193.82777796308 16474.948439671 15766.4172761037 13044.7644355422 64506.1154978056 33036.1170964732 89525.5252514998 925039.177785519 80684.3407028077 37827.6993447723 49895.3059747667 22802.7421121761 22077.3853918225 20649.1858746589 22673.1346878788 47206.4034204002 15825.975849051 20457.6601043274 34888.6637730799 9016.70647628017 5050.80224502684 4894.53759394563 4007.99173598878 9913.12950197874 8969.33920408352 2082.78136661148 162.368048124922 0 37.5091552098893 26117 229291 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5865.83246987811 9629.31831043105 9129.61318692518 15826.1496868507 14451.5225732257 14257.2646138857 67378.1002047323 35140.1839932099 79430.6862335957 971537.292199752 95048.1990009366 34250.822184866 53981.7802639923 22149.3629179231 22739.7404114112 19720.2739663701 22032.1921964203 46202.8484834023 15623.7162308854 21791.8282237893 35176.1711542114 9767.00192522416 5468.6143161509 4569.89776048758 4563.82358770422 8882.20625237556 8771.92387991741 2010.78610246961 235.381992889325 0 37.1292993583688 25786 226285.06 DEV 709.474595733579 1281.35133950876 1150.66018727249 1786.46942818895 1310.86500762062 1686.73908546989 27404.7607244878 9536.37111939394 32147.0049720647 55494.6382019752 28092.9397631172 8405.96006751478 3724.2694651029 1680.97842672911 2002.34656919952 1327.36934115837 1541.80060175494 2868.31592320301 2045.64576737813 1693.31845772161 2955.24202249436 829.224833920737 477.518284320644 1005.18728540577 417.248422019794 1762.01129753502 1680.67974548731 367.133465640884 77.7674615564755 0 8.80042709064314 230.876027339349 8752.27982850183 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #