Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-47_preproc.map (3418106 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7023.96144812707 8651.41124561968 9177.55934140787 25453.5072201278 78857.0032964315 19090.9799422307 22082.7882331558 39759.6059207649 39681.4468664351 896131.115681254 39834.9961357761 33306.4235237013 25133.1546889426 40600.7882019192 24462.9390066241 78796.345053408 17453.1731443753 123727.441768198 16123.0034309536 29837.5325356802 13718.3539109883 9300.6586898454 8897.84132561972 8802.1639934751 8380.15313806657 4891.67864452247 5214.30675057194 2430.29861224285 256.864741773643 0 30.260617336304 31411 231507 2 6850.19497315502 8371.33625977537 7205.39364960484 22851.9313380178 73852.6915598554 22601.4060741842 19317.4868526848 30856.9636944678 32259.6952765252 919259.643480757 45296.0182586695 29913.2186081278 21395.0694987297 33372.7417070311 19194.1256790429 82820.6657966787 14489.4090464306 124194.374013756 22225.0280316495 23290.1784349638 14118.1500301181 9295.42234099171 12163.2605521262 9093.90076533234 9661.33758593765 4472.13050124809 4575.14727095386 5080.41007486017 245.56084479442 0 30.6542271941869 31382 229114 3 6520.92199502273 8283.89917853977 8142.34397249077 22241.7087453644 77699.4983043626 15466.141868504 22261.2289034433 46765.1926027527 33444.5974260178 955437.488788369 31330.5254068589 29971.4646769458 27494.3789720056 39782.2107974062 22233.6253870692 75255.1506770118 14442.5446173313 123858.374140015 15288.2834721806 24392.3316718566 14371.4699474759 10071.7944357131 10836.3509291347 5867.93126688447 6855.9820034325 5059.47810809659 5442.79381852681 2770.69342383952 162.126319587897 0 32.1424962299367 31646 238324 4 6890.01743497603 8462.71220240225 9347.48265444482 21906.4662717299 78596.0786575595 21773.8423401988 19747.603475812 35056.0879141922 38193.6729015868 923108.486819717 47640.1883079741 32784.0298202385 26712.5801410053 43926.4110277255 22276.1939119617 84304.8621411653 16915.3190442324 123119.541942279 15415.8416181156 21885.1045596249 13232.666383909 9158.03826386864 11629.537287761 6274.07966126148 7392.31090209842 6561.85407290687 5466.81883333354 4121.50178893508 205.405132394327 0 30.4056822744079 31891 228505 5 6521.76076794694 8717.5516377033 7429.46816376092 18104.0904009064 75869.4734067509 17950.6662987073 20352.4522051871 36354.0331625959 34132.623945081 990901.732097931 34322.5170803616 28075.4269235886 24293.6755339194 37465.4770588532 23629.5118770731 75165.3630668257 18577.8956602156 139559.413788301 16833.7934753698 19522.6208494907 15905.9195961072 11300.4059432297 10959.8884325495 7515.15611913806 7419.12096186878 5133.70125512492 3897.95512831165 3211.05038567497 264.203718091757 0 30.7685214106841 31446 234771 6 6967.49561766627 8019.02502185919 8288.97125611532 25512.3737965892 68832.1639852218 19362.0848962763 19822.6169527874 36953.4063481704 36800.4935338198 969846.830949695 43791.2963561543 28305.1699358099 24891.0149808141 34956.0165415349 21926.9350188315 79381.9747313006 18528.7045213785 130789.849509482 16201.9232650366 24539.4381907456 15593.8578504942 10919.5921964508 12150.5683413779 7710.06192297059 6635.96661577593 5157.71739210866 4908.99131272817 1819.39154154246 361.220798830836 0 32.0235672648991 31211 232843 7 6762.34460382781 8654.0267136862 9978.1470648672 27771.355477039 79895.4489151063 16981.582261275 18984.2372309126 39837.6005963489 34835.911872252 995378.334761771 28571.9457933218 29068.8832118573 22242.5858217028 41386.8038556346 24435.6738723843 78960.5561381018 18738.9083428959 121300.270511348 12487.3065860555 20381.3558635433 12750.2911504869 10079.4914322354 10828.4690514612 7702.63732791747 7821.29018259992 6139.43476053828 6819.79578651971 3606.07480291719 289.748214649374 0 33.6492952208424 31754 246334 8 6847.13930258036 9070.01744498368 10128.6588239049 16082.1342494701 79303.3011332192 21155.534435039 19347.6915066951 32206.4502577995 33580.9510352426 1042933.64092708 33217.2093522399 29842.8222776558 23944.9244529691 38166.0810361695 26603.1948282227 82630.585383083 18719.2359431097 119000.664706724 15416.4443995077 25487.6405798704 14142.5203431228 11746.1655925882 10916.1599229137 9541.04243434136 7797.97909037383 7700.98687089023 3552.93421350297 3141.23035857454 218.151708501393 0 35.7798430368644 31842 250678 9 6679.66974252149 8145.27224905249 8644.34060671763 20273.3184941811 77571.8471620839 23858.4104277647 19966.6297748242 32336.3967716625 34173.0185277172 976958.753750737 30566.9691976597 33011.1455007028 25969.4213746989 34038.3565668609 18881.4300289503 68793.6775331283 16827.9300194513 132190.426349032 15785.0032023606 27768.8270524021 14159.554161505 11544.3545112663 11954.0564593411 6970.49649580059 7675.43514870696 5052.66927614841 6970.85439185823 2066.65236465957 185.441146608079 0 32.2478472229524 31516 234173 10 6722.32000735379 8750.84680162104 10097.767806878 18544.5920167827 80053.4254267475 17624.1854269799 21777.0179657485 37987.6298649934 33145.1141857068 1018511.05213193 41014.0040621789 29881.9488861194 23395.402853437 37694.624303637 22039.0618664806 81147.3168896565 24521.1408466078 122211.149385955 15704.2475428176 28417.6527075997 13412.1057367868 10486.4095704961 9597.84639468054 7178.91359680501 6916.1203081628 7144.21607452264 5026.16207053027 3263.87147686857 212.363652833776 0 34.4081084630057 31480 239600 11 6609.1545315726 7729.89743201369 10660.8086541871 22455.4378198508 76467.6723302023 18011.6048641864 20930.9682514083 43105.2831147929 34070.6493091559 934151.452461606 34816.5459597106 33879.2210677128 22914.400835881 42843.7992493599 22372.4387164774 74651.8952832988 15361.2476366128 103002.499794501 12853.7862509683 24499.1874946421 11340.8328901102 9203.97290026712 9089.45754105902 5657.98819382996 8172.84828215713 4388.65589360952 6446.83794351811 3427.64914194084 256.8778208105 0 35.7852052149573 31453 235318 12 6790.06463310492 7790.5322705068 8652.65401004058 23527.770231193 88496.5777497918 21549.8639233029 18856.0939455396 31696.4842790127 31008.8934653303 995544.486833326 37826.5428103291 27025.8676239125 23681.4514892373 35443.6672420078 18443.6960539308 79363.819151412 15311.1659236439 130326.977056792 18480.7166995121 18522.7957925485 13129.3521786584 10525.3284166276 10447.3275131845 8445.78535145161 6898.25367106758 5130.01554354676 6575.1779364303 4013.67626816958 290.89358109954 0 31.1351792012875 31158 221491 13 7076.13392898071 7485.60160659309 10468.9942187028 14186.8842667135 75549.2817334448 21027.7433405105 19645.2137485597 30218.6326198027 34711.8330639962 927489.526263 35796.145512288 29525.7452651309 26957.7503230096 33176.0564687564 21108.9994212989 71692.4213972605 15067.7368204765 113064.342352226 17341.3882169518 24816.6695365058 13525.8231037453 10536.4782776279 10028.134152653 9952.985379294 7115.91361431172 4686.13543985794 6479.07875925829 2576.51562816435 257.431694443297 0 34.1682575085097 31649 245480 14 6970.6172706525 8350.45852446718 9861.51360885941 18705.4891313724 76129.6853773565 21008.252115102 21125.1540899139 37592.6251238871 34839.3294600239 958608.380889252 40491.9080080334 30817.5244117456 25111.2791426404 33955.3181478053 22598.8824161628 82961.2457423567 17385.4890874097 131069.246571855 14474.4762051155 23550.6315984681 14075.7234693297 11546.5908935206 9707.99293921439 9133.91220544742 6972.75441773426 4401.01395461645 7235.96121921245 3789.50525677709 216.37974974001 0 30.7388790580535 31213 246119 15 6525.37855989551 8396.67279597088 10971.7192552622 21117.9837772206 80256.9836824982 17614.194102131 20343.0150915481 33958.1124180405 32680.9292278176 917686.919003636 53171.5614091813 28705.9679792447 23015.9719560284 36417.7037747254 24574.9638146277 87546.621157217 16999.870817628 120007.603571489 17773.813615165 24250.0049762081 14039.5964140884 10023.7580698438 11191.3318110104 9031.64796591279 7584.27711631892 5829.14364508909 6001.61015780246 3314.73496705099 189.859433687662 0 30.2227007341124 31365 226023 16 6563.80117848217 9358.48258952436 8043.84410727431 18853.8253420805 87269.8841619696 21281.5059999477 18940.9485903153 36213.0965364516 30562.9007751977 947678.691010266 38405.4414894947 27573.9232548595 30762.0183269256 36666.2070853816 20542.2770007737 75853.3366516344 17864.8023045186 119202.235622759 13674.5184222422 21569.4597014168 13945.5635667526 10646.0589560451 10253.03509559 7731.03182523231 9553.16830908649 6199.46378665508 5770.00037348137 3899.25440592957 181.861913529784 0 31.896933104991 31036 228226 17 7055.74438297981 10048.1545768559 8380.73701755156 20616.0499410053 87964.3147123111 17395.6462937517 20443.0083133425 35236.4923792873 32187.6639392934 1021648.75818785 31741.887025341 28578.0874074726 25983.2792780409 45527.069300857 18742.7327714225 69512.8917746889 18960.1212288839 126884.963536268 13703.2966485312 17744.8509929235 13559.2184349017 11125.7854111218 10197.7098960927 12956.0407551427 8098.94339881382 7782.83565199146 7004.76919828852 4529.79175177261 235.442037177426 0 33.2677073313859 32102 247106 18 7160.44353926745 7893.95831940652 12570.9819290437 20297.0367562575 73918.2788384469 21167.2231990771 20536.9404874211 36357.9474886209 33329.1476671686 919308.661753699 31183.9341386414 27515.585491837 25766.3582065977 41227.611248222 23047.0072410604 79331.3025804032 15599.7616467104 127037.840264968 16346.7274442815 35025.295595515 12872.6419512285 12701.7809595067 10084.5472598139 8163.76351240894 7165.15883564406 6258.09300120591 6034.5001650843 4323.80959950481 350.489218594434 0 30.8379913425947 31586 237459 19 6309.02693705723 8037.803589302 10707.310619135 25127.4471146787 79368.7796923145 19864.3576249038 18392.5996988554 30151.6211361441 34647.0773745459 936568.591102385 37236.9851491132 28178.6879553786 24062.1944499282 37772.3253476333 21308.8233166559 77113.7041617253 17668.1711599462 121987.501822526 16004.7767027235 23521.764560372 13828.7531974154 10893.7262436235 9847.79295081002 7829.51007345673 8233.64152249504 7232.60779501917 4277.84536473663 2703.02431086786 324.182447326713 0 31.9321954555993 31473 236755 20 6963.70336043373 8318.12325645098 9775.85645621013 19982.3632500591 72985.6488454513 22070.2146055869 19383.2487551287 32652.0719145561 33344.0205515031 906058.600642015 30822.288400723 29153.5581920958 22048.7149079057 33314.4385544547 21092.6231102782 69082.5513112005 18305.2604614079 126973.181220461 14210.8654907534 20429.4959911708 14041.7094215835 9572.11859012151 9657.24742477799 7613.91200353206 8703.13524872877 6938.29677820295 8089.47618510815 5076.71733460958 283.315817389762 0 31.0740659020716 31018 227718 21 6970.19267167919 7761.38051640094 7580.70938325567 22600.5045865853 79452.7572761363 19551.9661364832 18434.9164211173 34327.1545577848 35011.5322895084 994938.180478628 30294.7746862757 29020.6079628497 25612.8945061897 36594.320733699 24458.5357241713 64014.58754201 17029.4424298084 133040.327404502 12976.4021313426 26348.9703183427 13755.6656273906 10709.4411492027 11081.4747805368 7144.95651504552 7740.29628732472 5384.24267912288 6583.87823766881 3418.31766133942 280.322713457333 0 32.6763016489559 31821 245402 22 6517.86405930507 9080.84621448674 12516.9798431677 21541.6401000747 81413.7132934883 20386.4474598229 19609.4140048593 35119.1929249459 32786.2027510012 959869.918232097 37575.5160162871 31419.4444212974 25707.779898423 41510.6193985632 20572.016084235 80898.6658282884 12328.9051611653 121705.880549623 15731.9607156901 23265.9655038617 12900.1669034561 9418.16025312538 10149.2788938864 7104.18251162554 7817.92323540521 5985.85435452117 4294.52205245654 2049.3368919109 294.170723418383 0 32.1299018061531 31385 233192 23 6208.81409107743 8452.32868566735 9123.04936761782 21730.0371713103 82927.0732402443 17917.5486471973 20272.2128910119 38649.4027548341 32964.6509024757 962776.307903633 52614.4597237956 26388.8226598962 25340.5937413581 35695.8853633253 20740.9630814522 69112.7339507042 16676.5946483322 117532.892145985 13403.5316056603 26810.5723046093 13549.5829194451 11918.0413623651 11348.9319312232 7574.50537544724 8729.2534303489 8076.60940737468 5458.34128980278 5146.60459610565 237.32521162289 0 33.3949783931783 31763 247639 24 6791.89076389116 8064.54555654081 8596.83452000758 19264.3677103554 73118.3335910898 21879.0594907749 19610.5965221501 34909.3061284943 30346.6377818778 987470.264956301 46237.2555047036 27881.8357499058 27125.4281784283 35265.5116013316 23036.3502944707 79656.3808516845 18007.5201047052 129737.33453072 16230.7803470432 24722.0903550126 13431.0657490628 10250.3626836851 10367.5837868433 9399.32781478519 7480.49014390734 6982.26426010011 4073.94799947034 1902.12783052878 212.139250034094 0 32.4092714280311 31744 234987 25 6763.5575940356 8167.98216762504 8583.83483172281 21789.7503197964 82142.5982129614 16884.9214130218 19804.9325827589 32010.5652329126 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107521.802302006 16299.0102054111 19769.4685239671 14032.3607290994 9320.17619445785 11606.2196335855 7820.25945380895 7698.14876278672 7861.52067037723 4108.55178034883 4719.00346396039 215.919976216659 0 36.7478760449782 31963 246210 96 7115.66588457331 8119.1992094863 8515.78334841437 19731.7861437407 88128.5752801149 19234.3938272052 18434.8929840798 39020.9629103836 36810.1131379982 1021151.72926459 28098.9322471189 28682.7265994904 26555.5834065989 29567.1271908276 19917.178393595 69464.7663765058 16040.4885720075 122191.634173581 14568.7001616069 25504.0211981445 13695.9425308138 9186.88118578481 10580.4997525257 7472.2466874078 7403.78861951685 5990.95436427081 6004.43442686794 4200.28085188099 261.797013354443 0 34.2782168210946 31143 231363 97 6358.44299072653 8464.8149473035 9997.88458291849 20399.8863626712 82844.0265107572 23673.0897961841 23198.1861077357 37963.9263692671 36166.1217741669 952932.588844476 42349.8209048721 29663.1881854921 27422.9860077216 36473.7687840845 23622.7547858405 76472.0136620218 13101.1166816051 116419.932559188 15684.5307418931 23409.9837906319 14267.5017090499 9944.47157024824 9218.52330055367 7702.24642306393 6235.87160569844 5232.01163124592 5770.18339558199 3188.6474801106 260.80584214538 0 33.2147062846688 31236 235860 98 7001.80457454397 8299.17482191192 8280.39457727653 20519.7606449325 69591.6678088317 19424.1096614361 20370.0330013164 36840.8813825521 34548.5171456106 985599.384132662 37073.7987320875 29152.0282870733 23778.0267252951 40399.5109990423 18895.3609273231 67729.3355718224 18264.2653857991 118431.238768136 14781.2259743638 21054.914541819 13129.5851849801 10153.7685816913 11703.475083164 7290.50054378883 7973.57768331095 4914.24939989182 6306.6156243418 1576.96469033072 143.760573711638 0 36.0150017148215 31208 237778 99 6175.06998932557 7205.27626959118 9932.55504653404 24779.7055692494 86041.8953253368 13815.3437411879 20388.4465888368 32669.3166429834 32729.4841562091 893347.926304665 37442.8291158393 26956.335530988 24092.9618662072 39164.8124254195 20946.3474347394 68299.9410671293 20368.9632908636 111979.607301697 16101.3602316686 26660.5348558897 13533.9250144809 11585.438170568 9487.91617606351 7024.38416769679 7543.02118918455 7115.01700365331 3739.57900754657 2318.7607696608 330.192975007419 0 32.1441528950567 31086 230387 100 6995.16753824076 9140.94918120605 10372.0128483535 24152.4518390053 81165.2254291822 15764.1898745891 21687.0641380479 35164.4726703828 31785.6950805695 914451.030668581 43551.2030059645 33763.8348559122 22221.414225311 40997.8849089655 20678.6793347683 74139.7998183149 15699.9854579836 106411.72131107 13323.5979086093 29296.5241561431 13249.2420155774 9606.51800953159 9519.29609742667 7821.46288633344 7984.54634518087 6390.21125397357 2726.83827222441 4153.40360767585 243.823311033622 0 33.930496101473 31770 235779 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 6772.90558734616 8340.11286833236 9506.04720080904 21059.4850157269 80046.0004127865 19552.6935002083 20415.2770500114 35988.3220110809 34008.9869721093 958670.936955792 36491.0958952225 29453.9007251525 25850.0212140657 38519.4442339738 20892.2804711319 76872.79529607 17263.9942100403 122983.54812636 15366.1606298041 23708.1469139468 13767.3518421341 10364.6686579864 10556.2688601558 8068.86411628644 7841.14868186743 5860.03192046869 5631.52809223289 3553.47458653852 271.47844457679 0 32.3080640396887 31502.45 236471.39 DEV 363.427188190073 550.705126086895 1302.64830183005 2689.77787396793 4900.77927365934 2862.59311913462 1533.3797801994 3270.3121423226 2401.11603636791 41751.3194030714 5806.0204678406 2155.31299325567 2685.37212876068 3658.85279492962 2430.99480645107 6159.16499745233 2299.30356167888 9049.89845325484 1946.88031861737 4013.40946133499 905.984098914396 1072.01676421958 948.266879052569 1355.2011213012 695.886460716495 1038.11601373935 1266.45104883216 1077.07049767003 63.8837424817269 0 2.25204899779656 295.611040896648 7262.84434074557 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #