Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on POR-82_preproc.map (5874594 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7015.70256985778 16082.8548302074 10553.0488680487 4292.1415663544 12227.6408071426 15525.5396051505 24274.3905056 27627.5512246907 32474.7795913147 842453.896087137 33107.9327967175 18555.893919588 16093.5056620701 34460.8044318878 20685.8306567761 7782.40128109608 19594.9171721782 9420.21963107302 7598.18192613381 16570.9404790163 8496.35665459083 3790.51217745492 4077.06947186969 3929.41473538889 3022.97736567908 1794.25174043883 1294.55789438718 385.761479109156 63.7049833405767 0 91.4318224787297 20191 138049 2 7506.41207177661 22142.5979759635 12888.1043002116 4999.46469742007 10204.2826903505 12769.1781463788 22314.3048131517 24329.7511945778 36886.8959853246 826763.807842785 29352.4271830336 18264.2074852374 14401.2543119215 30869.1992106351 27586.0671080105 8517.24035099717 23033.2056829421 8694.47203679108 7799.29287307914 28099.3115271287 8002.41733645657 3459.30543904947 4799.54124821128 4108.94817577648 3187.85180218071 2055.96235022436 1220.75630034009 432.572810058691 162.375575037939 0 87.2874130842863 20561 137424 3 5619.7813380781 16526.61644591 10971.5311998814 4290.43800621696 11095.8810155521 16830.030408969 25835.4827774962 25295.1828854826 33597.6762377228 740708.536092873 30994.9953193675 20699.3998731571 14473.6549721625 28514.6846575262 18001.0874074643 8388.21043598207 24119.9038318783 8525.00973196318 5364.54397524052 19058.6700148033 6752.79826886201 4831.82525322898 12606.9161552898 3496.59806133327 2625.40596661439 1585.13810907409 1749.97215082628 460.423887013097 183.882538347766 0 82.2674490856639 19883 136099 4 5283.97668203506 12209.2810522802 9315.33454624379 4535.28444288543 11120.4725904688 15213.9393838028 24177.8020302757 26026.5188964403 29353.558920602 837390.991956074 31836.3599735875 20328.2051530853 14702.97697872 34352.4462646295 27017.1428712356 8751.83795149449 22982.065754243 8720.40597459008 5743.23507026395 26157.4258252645 9465.09911775158 3383.5760698276 6732.36499589726 4098.55649837974 3025.8663252638 1826.11519547111 1708.13877518493 330.322196705493 116.889410728099 0 86.4628606256059 20245 133422 5 5902.58797441601 17183.3061576011 9639.79201116578 4957.13644269968 10244.4555861969 11355.4519940848 21242.8920087444 30163.4725411624 36117.1123958267 819526.789436409 33226.5366042348 22408.4588939183 15952.4808172281 32590.4555305204 22027.9675217714 8260.92046512338 22733.9592029261 7915.38329943402 9982.76197145952 25211.9208765156 6100.78968760301 4358.30917636145 4302.09389214872 3508.09801018284 3844.40186055669 1632.19165547418 1986.71340591833 332.905452652443 137.636918709692 0 82.4022673881709 20251 134133 6 6607.9782470297 18030.0849094411 9302.81718152746 4509.5416188727 10999.054898585 11792.085183702 19506.1878567288 23696.528687887 31813.8894547798 766584.307610304 33635.7528720699 17586.3551153836 13878.8386856705 29546.6333517588 15283.4686315579 7895.09015694265 26448.5049797997 8526.15256637114 7947.13167823606 25487.0801549775 8101.16246996657 4093.7011917945 4513.86203054628 4056.18357421415 3706.4292448488 2262.45380592726 1834.39623166425 403.003382408584 183.201520067291 0 84.2768637553681 20118 135676 7 6356.2927492923 16858.3237136206 13561.9914731132 3709.30303070212 12083.4354906325 12211.8145989825 23406.0428723967 27292.4648892398 33870.7744067356 848699.155980965 32586.8040839398 17764.4571739712 14408.5206437774 29773.3768682338 16898.3443723619 8172.74522887357 22582.0531526315 8354.72832409069 6954.6425777255 27836.7845995977 8596.32812980395 3535.67323705468 3691.20928833217 3256.74901174316 3878.07064826317 1779.27003861668 1677.7127345314 473.387684602822 63.577082663863 0 90.8820825394592 20065 132344 8 6182.23469273285 19094.0401189941 12061.5542202897 4416.47725691541 10230.5363994487 13819.7428949652 22987.5211655203 26937.8658600937 33362.9689102084 867172.383983281 29087.4368301635 15930.3958597974 13353.593165673 30271.6807421391 22450.0335893638 9794.80764473109 23945.743288256 8985.69605233718 9562.57401777099 29234.4200594443 8711.57791169133 2953.64952697612 4788.27493361419 2758.64929519542 4440.63158454848 1692.23540932081 1217.71151821852 472.302487594572 119.645346570001 0 96.2234195558889 20576 137761 9 5380.67246016641 18461.2249883564 9979.08644123613 4212.10213379063 11440.5186811882 12209.4786837928 23622.4296953438 28713.7896700416 39053.6626960044 805894.928718018 31572.3120172508 23324.9894063406 15061.6784731216 30725.3983797821 20822.8833038253 8466.21252662596 23501.8907599029 8552.45420361466 7128.34781529053 15557.5196425648 9048.69398225164 3866.77805146289 3840.2574027791 4010.9417703017 3082.05785001687 1388.65081912273 1690.14220213571 320.641276013662 174.579363246522 0 80.8622565763714 20144 139151 10 5183.85279731591 13633.4576961941 10365.556111842 6134.56064158519 10059.7087285909 13697.4022514245 24936.7806917745 21350.6061130272 32288.9818407068 774344.36495444 39765.4776635386 20562.3726169671 13568.0795251652 29281.640001444 23462.8056209113 7472.73923167254 21218.2894731961 7857.25273636172 6366.52874248493 18669.6461529781 7248.89773922166 4590.89107333756 4426.73346849429 3136.81583099428 3919.62039474887 1952.69238510002 1510.04092368612 453.423366682346 146.886428367653 0 79.3365317154841 20446 140691 11 7349.60775878327 17774.7716013888 8554.6891516187 4675.72918127721 11202.5039110201 12308.4449108249 22553.6669866749 27916.1369771978 32095.8541291098 851118.653128136 30671.9515555277 25149.231843515 15978.9033001405 35619.1616086207 19276.0246060026 8105.57408277844 22607.7338312931 8953.69182826237 9711.00815475471 28360.7752281686 7284.19088694743 4029.90444198334 10891.8083767391 2763.55879646376 3076.98230611122 1493.06873416247 1166.3149352464 425.924475820415 131.353438985635 0 88.5878572653636 20606 142162 12 5647.51283945068 13358.9877480396 8740.6279364016 4707.37463868498 10683.664379171 12270.6948595156 19019.0076526624 19701.6704128875 33957.6685054384 886317.540274934 38156.7857619504 16805.5883299141 13559.6425369291 33069.7658212075 23958.8628271062 8063.12088231388 23752.2971688174 9028.74421305482 6788.02437014158 25141.5037133979 8530.36907604338 3865.74290886165 3745.75099989797 4097.51847300903 3198.26436260022 1748.28732721332 926.788220707272 309.402702248982 161.327466851864 0 88.1336858582763 20345 143501 13 6817.12888529758 17410.1265247686 10475.9870328947 5716.13177524278 10708.0139490982 13908.8039372377 24172.8947936608 29622.1859483752 34215.0008084218 831582.893433622 30566.2342056768 17158.6164482427 15713.9606857878 30193.7007319786 12450.9522808022 7355.26897917233 25639.0459200807 7551.69963939158 5272.33858000357 28306.5469133358 8390.27167073217 4892.7453401718 4877.32922066365 3282.62857335863 3109.60247736591 2417.12309852565 1556.65869094335 472.68399596803 120.72884193133 0 85.3775510768744 20486 140486 14 5195.22727182628 16240.4734985783 10132.7208801754 4704.50946586235 10006.4354175463 13513.1392219516 25019.1122326123 25169.3310911896 31023.4888571116 835896.843030137 35543.8501178775 23528.614951605 15810.9105589469 32803.4827741104 19623.3069126597 8162.38628002827 23972.1125855105 8706.58294609052 7200.76284187415 27097.7746756783 8678.78230781942 3854.5701890621 5706.68668964892 3170.2022414498 3594.05423648552 1916.35059674224 1315.45104274445 420.727928432445 86.3767478774456 0 85.3847290788835 20685 134961 15 5462.423409765 13270.3857850552 8619.32704009944 4336.64984830134 10621.1791260972 12107.0938723523 21465.0858647628 22766.1015146693 32201.5095556894 844863.650335081 36125.2158451831 17987.8557691183 14833.210203506 31380.1177764066 26366.7826020338 7454.33564721276 21797.495831645 8580.28924405121 10413.1089653142 18869.6465921299 10605.114606527 3869.76031487867 3232.50006367705 4029.48224394581 3085.32333697156 1280.67248488603 1528.44711829278 407.607183514511 77.4087789985193 0 89.528741484186 20159 132225 16 5353.29882588171 16674.8829136155 8982.09403944458 5281.92564567957 10893.3271772491 11831.1087271118 21782.2651932901 30120.1001388862 31498.3946865071 823682.565557433 31840.5481035892 19629.7681692942 14374.5336557431 30086.5438755831 21100.3225481379 6975.42149627203 20810.3474093619 8173.05455854507 7874.0836766061 28294.1071152058 9106.43500232696 4695.35620699134 5450.68277262187 3451.66181340463 3892.70518642939 2534.57145739664 1422.45548357872 478.311451594199 138.112524873654 0 87.0825373216187 20155 129211 17 5813.0905576937 16207.053894059 9079.26650970267 5906.9472678198 10517.633791151 13840.923384028 20654.7810223498 28251.1058718157 29992.3711080698 833152.516097438 34378.7356734588 18225.6519506816 13868.2269273335 32764.5243515953 12474.9056205958 8610.24767294834 23163.7400182295 7188.42023587133 6646.86092038068 25235.4723564133 7341.30317999655 3798.35787562154 4070.68555416915 3420.64213368202 3724.89264109693 1250.78605493382 1385.58541828928 449.613687404704 69.2597032699345 0 88.0009382513134 20110 138035 18 5598.93330904238 15893.3942770476 11581.3171404685 4579.48013832657 12789.5225853253 14510.6353107619 20551.5129194151 26233.5509567087 33241.0626894577 785074.582012648 29512.8376281773 19579.9101174717 12699.1621329842 32325.4616086553 20514.1692607885 7956.48601882878 21717.3071017589 8437.41632112827 9455.0830176386 23204.214557124 8653.94753691572 6274.88470509713 4562.97131397871 3673.28142646503 3850.98504399634 1934.16414085814 1438.23819899778 511.884371016684 57.1289103947701 0 89.4564005697873 20336 139442 19 3883.30563588238 14468.5170033396 10093.0510088245 4193.04108255476 10950.1691720512 12603.7701106944 29462.9592304938 23833.1908112429 31293.5925718311 847311.881439717 30456.1780172902 20514.7917574411 12713.3437438698 33133.221441569 19414.2426155773 7570.7287687376 21257.9679567521 8528.77160852264 6326.00434633824 14703.7018781264 6863.05748476548 4453.50639973687 5796.49935184547 3521.03716878373 3806.18902203319 1871.04869159738 1940.6873789545 476.04452140174 144.367623366476 0 89.817109406318 20382 136650 20 4707.31029302622 13627.0773867738 11166.1323379633 4814.15723117268 10466.5039746768 13926.1747259179 19662.8119501776 31703.6881483637 32940.4671981544 878795.012717576 31556.0948838222 19444.9301294593 14040.9391105521 35133.5998451391 21670.3667582068 8500.5084428748 22605.3021083567 8363.97200590208 7031.37837439392 26907.6860104688 8083.9144783107 4901.76541278201 6226.14486893992 3248.36532821819 3470.70179255855 1711.52368189741 1766.34724916783 376.107396812184 93.8769860964093 0 88.0849331371821 20189 136967 21 5175.77667823683 13763.3039656643 9259.11550361336 4495.55038612849 9911.72656544839 11341.1415187338 25087.4651258939 29862.9046973119 31543.6389626885 821347.830425939 32790.7104329989 19176.1074549059 12978.0880848395 31053.0854496308 20275.5077948351 8454.62175867436 21752.5247237184 9729.20175861916 8107.19681041379 22556.8889963249 8768.24937767491 4183.60908077827 4355.89768338496 3838.26789455517 2633.36351337557 1423.18547222257 1039.12160083566 320.367857921404 131.13655324875 0 86.8929559985461 20487 143414 22 5036.67401647687 13650.1202282961 9226.14359934705 4085.50564438417 11535.4710435073 12499.7922656146 19487.0589644727 27086.1278985594 33997.4431983473 821476.18941436 31447.0355494264 17625.0552729818 14546.3602185548 28809.8379895266 16830.2366523225 7256.04497653244 24466.4349718686 8320.11457893251 5278.65851407857 23901.3213226312 8162.48856702653 4789.12255114583 4611.34528529787 2843.0049879909 3287.01586561454 1429.72512063848 1550.4301275233 450.31672210741 85.2206817425054 0 87.9620865465022 20334 136982 23 5359.31666411694 16558.3253324281 11618.8165440668 4434.69862301705 9338.30470273389 11544.8068100537 21917.7274769825 24307.4307048845 31161.6787289256 829795.638560562 29639.9754337539 19173.8939711254 13690.5554233471 27977.3351233249 17320.1570337749 7767.27061652909 21134.3577362536 8033.53058333834 7486.50593272548 20836.2532540113 7646.31274812953 3897.69601780357 3288.44461565122 3227.61255607536 2852.03780263149 2403.91254986648 1481.03653254126 402.834710141251 135.434577690145 0 99.7687862868575 19990 134124 24 5258.94111574478 16664.1057905208 8846.84597045104 5846.28566623433 12362.0182145295 11276.0086881585 22082.8423035116 27299.7513855959 34419.3306981253 865094.657832686 29994.3123581089 21358.1082430102 13830.8869118484 29583.6861880906 19397.1267225554 7575.59886935562 23654.7522927033 9712.71815290236 7802.91462208767 28841.890007102 6812.41779033106 3382.36479062463 4116.60035756412 3196.84172931888 3040.80282924235 2135.92092538622 1375.117096622 453.839298903392 122.961697746483 0 92.8444759518164 20169 129590 25 5016.56212800191 16323.2211068625 8791.35148848243 4548.02780978459 9029.36707998894 10296.9358634861 24202.1540902663 26626.4324777082 31755.2502593871 810952.589646429 32819.7769057153 27075.597053937 14152.1975542219 29070.7356231222 14716.8444004531 7576.10431432628 22468.5628419233 9517.20698626688 5944.12550551227 22637.9501303981 8422.51982048971 3964.53698527474 4531.498813237 3690.83114324717 3692.91679422797 1552.93075781818 1672.34885862787 476.123488269534 197.462611354832 0 84.5183934236277 20097 134155 26 5489.85200432304 17644.2911561963 7991.58444333563 4889.2173802589 10817.0303064905 14207.895153002 18166.2434447587 26756.0238803423 33664.1910258659 805646.141821052 31822.7610887993 20381.1683170658 16488.9086804684 33348.4765471394 17982.9049079188 8453.21410157253 24726.1548239237 7486.24619879398 6495.19391773359 19918.2199134127 8687.07666932411 3630.04345845988 4426.21165575043 3206.62625374609 3348.41353444154 1906.59319131017 1559.04706464279 361.676288320537 141.218186049439 0 85.3342492170479 20684 138020 27 5937.33462710542 14788.9606178121 9448.28581232634 5100.20744669388 10376.5064021467 13447.3321117118 20480.0140546373 33938.5839732662 31269.558644156 844148.98750479 30430.0412587114 17167.8190123404 13875.1588560317 32409.8326395983 22371.3840877634 6856.83135132202 21716.3099288315 6782.38042184876 7059.20440507519 24525.3531435054 6564.21818235095 4071.55379761162 3976.49003298843 4111.02296545166 3001.43938423904 1600.7770757389 1201.231278197 379.474650342219 82.9653588628435 0 86.7769935593627 20460 143019 28 4871.67895113445 17304.8196618243 12586.3940497872 5495.02696773826 9457.82163066845 14135.7508835979 21502.5407884304 23220.6596306765 37185.2836043951 796585.285983711 32884.4157271932 18838.1195895772 13093.5976629293 33808.4838409978 12820.6968699324 8154.21162596191 22907.4081232309 8598.13616569038 5603.1601682747 19662.8621645805 6970.62691498763 4113.61316482833 4597.01295458608 4139.19155855569 3464.35376445868 1910.94682276586 1235.81224730898 405.783314810611 103.220417554614 0 81.8897304024119 20130 134719 29 4414.86870139942 15044.023864018 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8067.06694423916 7824.66028597881 25629.1573610811 8003.42404555676 4249.57598985332 3911.50132213049 3351.60950496434 3276.04865209378 1985.25540159731 996.096997386353 432.47518542892 84.75 0 79.7003529657955 20636 140388 96 4269.25781643859 15286.8473039405 10043.5069372296 4598.97085220692 10504.900142997 14472.6426138331 22602.1571429519 23832.7793948868 29803.7849125814 803535.05052977 34014.5780445834 17213.4391421189 13102.842749819 29476.4829549838 21818.5455308292 10154.1051849621 21743.8595919215 7708.14723097953 6252.75443675584 27811.7910938678 7084.41769998604 4883.82852250474 4486.75302879878 4069.17308969773 3656.05565373474 1967.38773015127 1182.29676302512 451.707975425834 135.95622170182 0 87.6586005033784 20216 132469 97 6415.85473038724 16137.6217139794 8507.75273495151 5088.05668520627 7928.40251370516 10868.1926805449 20680.3665909954 25259.0271629559 33135.9581011793 778797.205133796 31968.4542927446 18657.7245687486 13474.2848550888 30005.0163675422 14625.7591990972 8313.44437380985 24545.1073066361 7300.21467718109 5927.10238975371 22164.6224526859 7266.85391666914 4759.37397856755 4383.29105306813 2631.87606656745 2689.14154360299 1580.59890837992 1524.10760019318 440.74971159337 92.835151147113 0 83.8476615459509 19710 126801 98 4776.02458888051 15532.4886832975 10861.0983612286 4135.09277353095 13016.9724653418 15726.1398686458 20162.3461822718 24499.5683842689 31394.3536406324 899576.15749425 31916.9239512016 20226.9130534714 13371.9133142087 31479.2998662613 29481.2234917385 8466.83000227438 23040.9671756803 6822.71940704708 6568.63829188356 11860.1664283889 7203.8051494988 3933.58472830513 4379.18238198778 2681.14590871518 2638.80846672468 1686.16753398349 1416.93906968259 339.680153144838 145.329832326452 0 96.0147126525333 20213 141070 99 5084.88162343717 15000.4839783235 12874.0349622328 4792.3547987564 10491.6075078091 13424.2955867679 22967.5925422892 24726.5821309227 33106.0613078025 803002.426498036 32415.2946284943 19652.7090224421 13590.7780349292 26588.2880552629 24306.539664798 8073.66052285121 20843.8650296964 8900.95247426461 6179.55506575052 21043.7577776855 8247.96863688094 3846.64242970827 4025.56791715003 3092.10516510858 3074.84515873939 1481.40934264006 1762.34353980311 359.225827743129 219.452689775934 0 91.0117940760155 20524 141243 100 4783.87480173563 15227.7101951165 8872.23585219674 4561.01222226074 10995.5282645269 15141.9793312419 18567.8486413948 24042.477555112 33574.2947060725 747465.522604084 28692.7573438034 22676.1942353143 16583.0221602228 31124.1287201174 20537.9778520106 9799.68849650512 22574.7210644344 8791.65973410969 6639.5072819185 19047.3248172248 8307.83610695998 3916.83168200447 6047.29247314896 3355.25063341771 2962.70584006166 1687.45096258527 1500.4692172929 316.198112771866 178.303089597554 0 85.6428389573763 20441 143025 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5608.0101340459 16124.5880143302 9837.82602277062 4735.00174567699 10836.6403427673 13407.0031706803 21846.1875680927 26561.4201958969 32916.9811804158 814542.546466749 31887.5050031931 19295.8144170981 14196.8546740232 30809.8527547804 19967.5424272295 8067.41557930936 22583.0460765957 8208.60661544321 6894.09835030951 23214.0519152495 7814.68459868286 4057.11461622243 4933.48769068648 3478.2880817403 3411.29685194816 1743.49311266268 1444.0438875408 421.543335264664 133.830525280473 0 87.2653679214451 20278.53 137009.47 DEV 873.695719594356 2310.73256697359 1344.60901959992 542.618377282283 1054.50672229548 1445.15511865824 2684.25920733145 3204.35208110321 2055.91673548724 34796.5751375907 2255.63396922466 2222.66997663429 1108.87131411408 2033.1342698238 4146.31322558809 737.361590977729 1684.05278227053 728.80415144772 1169.67827315718 4188.30942004449 827.00638548569 626.437809307639 1515.80203790929 409.422753217395 419.546015618915 295.169731305354 261.944342682947 70.680993928237 47.3693607794373 0 5.43740625327009 230.973827738123 3972.22018134695 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #