Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on POR-79_preproc.map (4745025 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 4415.30179555 4056.66819669836 4634.73616620695 3179.80742882506 10354.9187988538 9827.6263970797 12903.3003547554 21610.2977270146 18488.6925600109 520771.279820183 17327.4284310472 15225.5197689987 6806.86927567629 12021.0327716149 11357.5568786634 4406.0268777146 8954.05433669362 4743.24130719914 5234.81097584748 4362.18300294832 4770.81700619964 2654.152075903 2794.33105216409 2435.61456189165 3578.80805585995 1311.31687073694 928.713761098504 203.329684558709 85.5138196019148 0 93.8850010087056 18532 121162 2 3336.16355428162 5516.86969288522 4867.22254565402 2559.65077686208 11133.4614300464 12736.9623790032 10991.9711654733 18228.79263508 19158.0309938302 541932.087064967 22685.9394524366 14679.2078425515 6473.54765914521 12885.4465652872 16116.3908360466 4389.87433311533 9142.83514252347 3927.07714104775 5167.99735254746 5800.39162668876 4252.85030196309 3128.5455433109 2899.48340678809 1983.00854134165 4112.63110609262 1331.37059213371 820.573063426601 154.257372457753 46.5566719398469 0 90.3489727610269 18191 120882 3 4311.93050387989 6327.05651766654 7110.00089206142 2624.82953078699 9563.74086553502 12081.6380586897 10490.6732488514 18066.5550019359 18670.8133347737 475117.71171905 17104.4718179223 12961.2584126171 6778.10719979693 13825.9779523099 9737.32303399202 3513.35417609472 8654.15884127366 4382.43967286834 5298.55502228852 3185.26101480844 5564.4169579826 2679.14026202547 2230.49812833893 2939.02612873781 3357.3135553567 1367.818198807 817.84783550852 243.379168175103 46.7937196465422 0 93.7203339773416 18090 113284 4 3922.32109300413 3986.73366354614 4532.02764804576 3303.94328280956 12100.5381341369 12905.7170939002 12445.397239668 23389.3751606115 17534.5709532696 600627.790619741 21175.7373909373 11769.7188080268 6469.66409578307 14361.4581746003 11069.6041694997 4124.61780008016 9434.41167966151 4839.86949441109 4955.6078933408 6527.3295170881 4976.85689783726 2466.16304295082 2495.56231108892 2567.48844851311 2875.98895926378 1062.65500319929 1169.79267093962 594.839073747988 106.106246947497 0 100.049748056112 18396 123604 5 3408.83465820232 6463.68992728573 6770.39649941946 2927.76044399631 11313.4764636395 13485.1183392723 11409.5335413348 26014.2103983378 18292.2764731494 500964.567214262 25323.2365085116 12660.2699988605 6521.75840038076 14281.7511642211 16345.6046828197 4239.04719735958 9112.22402510014 4872.62231490118 5556.63296848898 5019.09911947103 4512.01485038603 2555.57333302902 2685.31245969568 2437.05079473295 2921.09806673732 1507.96048763784 861.488543100378 296.086927543373 41.9627826976886 0 72.6749836839283 18098 121020 6 3818.78851901741 3408.53511812504 9731.34236481988 3031.4569802755 11674.0099260866 11727.4809600602 10248.3802613005 21258.2523723746 20773.0407051959 543201.464136825 18374.8784911742 14101.9140624798 7168.65877536956 15514.7818286895 16282.9702219564 4681.38550656506 8840.20981620932 4369.95479895219 5065.50842731971 4730.68327931992 4908.18372384826 2938.22893021172 3867.27985562547 2331.59418708657 3044.32558677477 1028.59798921701 1097.08567378349 625.130541528893 41.5809115401844 0 90.6976538756318 18286 127200 7 3624.36506368505 3998.89656844964 2417.7101337066 2134.74708328568 12413.3122481563 12758.3945530696 10995.9646877654 20183.8414271865 19328.9069124032 555504.034656609 19125.274691757 13115.1795820849 6975.48408469027 12212.6627440104 15273.5710454129 4860.43679913403 9098.79792922133 4262.10313547242 5275.10861250221 5698.70700702174 4278.54039282751 2928.64309798977 2343.07583614058 2476.83997648646 2751.42946021241 1156.84720435945 908.061303412229 293.64616154117 63.1928681900111 0 94.421985896741 18123 120738 8 4072.81346864229 4088.98069127301 9554.16030829767 3058.84448777775 9691.58739062065 12468.0134315392 11827.7755010653 19917.7212195679 17824.2650260967 551748.398808488 21953.5317131075 15786.5875994563 6330.79867222934 12793.3368807433 13900.6412229822 4744.66811718395 12131.1864509159 4157.50081107428 5073.43130658167 6340.52840010854 5009.16213900159 2623.46474130674 2619.4906047737 2348.04644020169 2748.85340058966 1113.77426538179 1042.27277134984 200.947040490395 68.1960097768334 0 96.1393878466267 18369 122738 9 4610.87412772542 5890.13775411258 2380.98008231865 2863.48002202389 10603.7610169622 12334.0164891605 11628.5141219689 21695.255831345 18055.550656257 495963.340397591 22736.0060157948 14618.0830109046 6289.03469804914 14579.2251185433 14787.7252420206 4664.66301598257 10696.7575222807 3836.23934793656 4939.71080379267 3354.67399459325 4853.1678830174 2504.02547476706 2237.45824342271 2535.43537819394 3135.08526268291 1141.69699972748 1197.56035705614 308.859819507906 20.0444784580499 0 77.7101559460914 18348 126197 10 2775.19430054827 4425.0169680731 4931.21869285754 2569.95200836342 10317.2084495081 13335.6415067921 11429.3324401679 20973.310849732 17572.4654040595 500991.01167794 21149.0862334322 12132.6186757968 7806.27379913895 13528.6288745827 10437.3001822007 4476.84916020329 9784.90880548631 3766.43857293457 5171.032925162 3577.39697449463 4608.56172613804 2189.3949372774 3136.33987535338 2384.42454777267 2837.46721256567 1473.06998391169 970.357110219576 624.376448492399 52.449880952381 0 85.3304426436512 18370 125755 11 4864.88485582217 5169.47278457004 5175.05115882546 2755.46996578879 7877.69327933656 10926.8419959039 11259.0538359667 17141.1329817469 17098.8298188289 509827.512532659 24193.2465220212 13035.807343214 8610.00895579386 16123.819930489 14819.7498017562 5295.22778354038 11517.8553093921 4536.27566074157 6087.90916537746 5542.99674583305 4753.22492593981 2401.39635726479 2802.14130105516 2245.71544744943 2699.46856999424 997.439023011597 1281.11733576649 610.1139006329 63.4719805194806 0 89.195773909734 18298 121203 12 3846.86802634894 6267.04269354568 2776.75417799255 2891.29035393586 10651.0303847347 11149.090106744 12566.2903543478 20795.2442739827 19921.165367054 544199.874591525 26142.7106007249 11601.8851592755 6999.67098469289 15012.1598468132 13993.418449352 5082.72625237861 9353.17227140817 4741.08212513065 5722.72629389919 6086.57767496858 4739.96325909276 2152.94557261109 2531.49866225689 2604.54449074863 2962.22006758623 1240.23103805863 677.253618625559 212.28615769013 37.9929723739714 0 83.583966832275 18197 121510 13 4115.84005866593 4271.31481831344 7802.11738745053 3873.8540959942 10725.5595047909 12688.5801598864 12228.9923904784 20910.0644383601 19028.2465518408 551231.372543615 21149.9940888836 15195.2631256982 6376.1612941602 13963.6115495281 12136.012089997 4043.12881636652 8661.08265343819 3725.85361430886 5094.06606821908 6033.81081979619 4039.93394700515 2500.61846121623 2420.84815725965 2322.84993932665 3180.07448012229 1286.75090264917 834.456087056508 205.176730609223 59.5036185767637 0 94.4233901336535 18169 127315 14 3828.62222088627 5533.19840825589 4938.2006313136 3146.457104621 10551.6220291334 14003.4539601406 11851.2811173632 20554.5086410806 20619.6871298809 477002.953325935 19689.624679544 17442.3927043201 6314.89464223031 15613.6079261174 14250.8077899744 4364.13913454032 10079.7250735537 3969.96355055237 5133.38320626116 3791.52516862135 5332.56009828609 2818.33135419887 2611.52770993745 2224.8320741244 3043.47201776229 1079.86311081138 946.212760420769 202.184470476626 32.4210217568927 0 76.0435411657503 18323 122438 15 3991.76913352939 4949.3709237552 7172.13562659569 3575.55401203046 10803.4873485568 12852.4853211929 9871.88894182705 21295.6593680525 19935.038843691 517456.662772201 20351.605108669 14458.4158833507 6697.94394036677 12864.9443827179 13012.9676377563 3819.89729876142 9365.94297332336 4523.43306674612 5421.74638735459 4050.51651371647 4572.72026206139 2442.98450374603 2479.23422306767 2661.7453117303 3279.77322169717 1081.50539436596 1084.55173220374 208.566204959693 54.6681327160494 0 88.4100433939776 17903 117990 16 5275.54748829451 5434.21754872126 7224.44474245166 2727.23467353154 10129.1641886692 12914.4946934572 12970.9148167935 21334.2148740808 18852.7757090468 490685.650643267 19258.276900313 13410.397722842 6046.7540219233 13164.488813873 13330.2081935168 4536.45375864225 13045.2857745139 4730.95404933299 4796.30075592456 3791.79242256509 5979.74947052884 2331.80976266204 2501.29713990309 2528.15972566913 3199.37506460451 1318.67872375203 1063.46148403195 176.389572486783 55.7131364924271 0 85.9373966980867 18328 121332 17 4195.2513324283 4294.56750097248 4833.9408483026 3211.90975810047 10914.3156608579 10941.1343546682 11508.6075496881 19999.885131542 21904.0895395293 482909.95902863 24201.2561337741 13504.5357926464 6689.30718300841 19391.0638361023 11547.3160311288 5062.59357264459 11774.162710251 4742.35360115503 5685.16552970441 4830.56559071367 4886.24998286219 2585.36112684481 2497.78441810297 2431.8777070539 2988.62546539298 1384.92638441849 971.429356531179 213.310063174271 65.1888431013432 0 72.9769533095357 18172 119237 18 4082.75891257034 4976.66333733278 7507.778025877 3248.85964262342 11216.0479436347 12671.7456076223 12049.5144670828 19034.3509882809 18670.5849395265 519957.643209199 22333.8487421311 15585.9291536569 7267.73450329734 12889.4589206116 9668.03318968203 5024.80170776475 7903.99282653134 4943.01576221755 5152.78135782366 5288.82876592049 4263.75357234114 2147.19179209791 2563.9401349805 2000.05926045516 3034.62988057849 1175.97887598256 712.821117035264 1101.75970105037 48.175 0 92.2002396830272 18267 119446 19 2928.14950256874 6162.59044288759 2337.90122570736 3071.29603952736 10251.2573323039 10659.9286527327 11173.9724873221 21381.4980276512 18082.1857920613 477443.578965901 18731.8031054165 12680.4974994721 6761.71389530313 15020.4326776846 13960.038665609 4686.45382080033 9108.37321502329 4767.61803985323 5325.71171409426 4966.37583586803 4731.24394775007 2161.82229194297 2625.35485948399 2345.79052358642 3351.19555595472 1278.51386961442 953.735734136082 180.613296339821 62.7423996105447 0 83.031030918776 18084 127759 20 3808.89863634971 4764.14651304537 2448.32849505813 2567.70357979002 11871.417383451 12438.1125402423 10725.7143916852 20696.5161601396 19627.2140289746 546920.123310321 24800.6122523719 12638.9262833441 6401.89063287272 15907.8847798779 11711.731252976 4706.30003409428 10710.0738104539 4262.90676116225 5858.41344109764 5322.89212884162 4865.29103845577 2475.49248824143 2549.49995605872 2663.54472974125 2742.16814026753 1132.79341693459 788.00135371818 160.222026115122 60.3114498916658 0 84.2069786517587 17996 120403 21 4088.60766386193 7532.1874531125 9908.66678414902 2759.6419142597 11273.4752907287 13836.5809795732 13264.3707983522 22021.2236393449 18086.2892155707 506591.380672959 23182.1821471295 16323.3421398382 6178.51858414713 14603.2681009626 17575.0970353037 4431.79062997516 10293.7747226852 4847.3628369092 4948.62320792194 4553.84654919487 5761.0244290606 2060.67335788968 2596.26049754067 3187.40672444778 3021.4468697905 1390.71613009056 829.091796190917 194.471481239711 55.119938271605 0 79.9661250012551 18333 119437 22 4098.92777829324 5409.24744348812 2853.24882468486 2262.78220799192 11464.9753271536 11951.5761257734 10712.4665089399 19577.5557386569 19571.8312194302 550080.529639389 19527.8817869077 13118.6670331224 5929.07571938026 15628.3350959008 11412.4100306106 3949.93547333342 8928.01123279459 4717.17546180381 4986.33937071024 4692.15050475535 5406.24170249542 2457.15251645903 2907.82649118093 2236.51789085496 3147.63036347817 1726.58927767456 867.677223528646 140.212199564003 49.561861348528 0 94.4845218949039 18232 120160 23 3896.44002647959 4749.20758795065 4671.19333993932 2803.92695966327 10909.9781758905 12062.7729055025 12940.527339661 17927.7812932552 17883.338199023 505077.21311448 19151.9844830686 14532.8894542538 6145.19849561353 12546.0563964653 15744.5985392127 5066.63572263756 10063.437408072 4890.94502894977 5072.757379427 5228.01819922066 5038.66926355163 2738.94236230971 2268.73947871594 2357.81842689797 3613.04189844128 1504.11053617209 1232.02538920441 572.491886694477 46.2469915898266 0 92.9752465846284 18131 118003 24 3571.10093519494 4318.31653550752 7055.31694418693 2947.23590838801 8844.35976627113 12899.211721264 11915.4844546121 22609.1080007288 18378.6808361752 505651.095651856 22698.5341025139 12636.982778386 6648.90668638666 15463.7743426746 14398.854007276 4750.69924500473 8507.90251294939 4533.31571030061 5875.70112762328 5978.1547174592 4529.42834149212 2070.08818023731 2652.11363632265 2477.62400847879 2728.08957152704 1104.30288693867 1189.02855737253 288.070352021228 68.8306905460131 0 81.9016757647839 18564 125056 25 4094.20651835142 9252.18116526986 2318.67717624832 2590.01635150214 9674.61949026069 11991.2324975364 10769.7734599488 20461.4865313698 17410.8735311332 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3444.54067288428 5944.87773782812 2207.05553732202 2711.52407963945 2386.91079140061 2955.16536835807 1056.11727678454 701.941382655713 179.379498039437 64.6881840353844 0 91.3515620303088 18386 123388 94 3649.22394700249 5913.56665547031 7343.85026985922 2960.56293140758 10041.6703250498 11964.6099598451 10137.4426026453 19278.2025950155 21079.3496929358 501424.845761241 15463.4372732496 14751.8434661225 5963.19500915059 14683.1865604433 11360.8651348489 5050.15342384253 12358.0756338789 4875.05505750359 5157.16348004967 4028.92576019331 4708.87188712037 2382.86147780528 2676.19518425474 2243.74481390998 2947.75139800278 1304.30465902332 935.531311349466 1020.01605627804 32.2300120444072 0 92.7805173068451 18044 119115 95 4231.17556278811 4692.62030740925 5386.73090714394 2509.35811532825 10548.1683581785 12658.4970478505 10819.5165432778 22621.4835295519 19290.0876847974 522701.915267183 19852.0206897944 13612.560016748 6346.49595351437 12032.9589950701 15361.0785490068 5230.27211830693 9811.75740829659 4233.69494961556 5193.90682752702 3208.17905975776 4754.77872828656 2342.49761401617 3160.2545521331 2351.48494813692 3089.39648178811 1193.50471033186 848.048087010817 236.351432797101 51.8611691086691 0 86.1160080512009 18199 119919 96 4276.66196386185 3761.53115664694 7364.15157646947 2689.59093479445 12018.5053268587 9645.10003531644 10793.6397370214 20133.6733576563 19425.9604417093 479862.585611509 19112.6430839071 15540.5349209849 8867.5762168877 13583.6411853739 13432.6039289981 4922.1923907622 8813.70728419092 5347.53710647251 5693.39769710593 5449.92465658456 4781.88506146158 2420.35796398459 2791.08664885065 2702.85308607757 2493.82273051938 1194.98531761855 1006.8135972366 606.911454553949 74.3820038491121 0 86.8396574160475 18097 122215 97 4274.44753358853 5307.90662742597 4935.18351352117 3119.49129326969 11611.3510324527 14095.5344188625 9918.15959146086 19498.1294704868 21241.4431998128 558670.682919329 18099.8700970861 14869.4426836239 6893.63212499798 15075.8011792354 16396.1387892898 4066.71956237885 10729.0147782499 3966.8256130542 5783.35087392538 4115.00935321863 5114.85699620958 2918.07714853199 3372.94523619512 2316.45441407526 3035.91856265057 1210.84975096393 1098.43172213147 303.601193384936 60.6501349904576 0 93.5473170924789 18295 118342 98 4304.7050973719 5191.15277346728 5205.42022126829 2812.55736758411 11117.4752875966 12034.7639803192 12161.7815001454 21709.7018895941 18334.6065092513 504531.432268381 22194.3436386727 13761.6086683764 6102.0403249611 13251.6472581449 9389.23713741879 4725.26113580955 8367.62540276945 4828.9493952458 5546.74033055885 8225.17809334367 4974.56701404863 2639.32584453641 2415.21754341192 2656.09022614371 3391.15235640559 1085.72049868299 576.854522701187 282.437446638997 49.6854070484609 0 86.8570518130654 18386 119987 99 3748.73657304465 4828.30070889541 4711.21770941576 3027.79954719015 11272.1710395446 11703.6842915694 10519.4443084711 21949.6669453736 21851.5297786359 526166.905532312 20426.4346611285 12505.0104226363 6922.78518987492 13225.5221651646 16063.2006885874 4239.50895533885 11196.7068398946 4395.82729454926 5594.25488448466 4706.57623669593 4547.05817189656 2295.56061444683 2642.31269735125 2176.40216498704 3610.8889718583 1564.69061632746 689.082493049102 800.850714279333 58.4665476190477 0 84.1123238613035 18085 121554 100 3195.0922585096 3245.63904188682 5037.07146681984 2081.53339618307 9929.52297655691 13627.4002268977 12002.4939194915 20143.1363322565 18791.2473587306 505193.495234126 21813.5285211495 15569.5132492831 6644.04481363157 13898.1372594095 14926.4527903194 4703.62750817263 8923.71993506098 5181.07758231452 5366.75509734438 5750.45387938071 5336.82741764754 2823.61928887381 3292.39966560988 2840.36768875128 3094.26841723824 1319.08239559541 1010.60156574398 214.957819040981 41.1518820861678 0 82.1754293068774 18077 118796 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 4013.93392559549 5093.35754927986 5035.65943930672 2850.5727905397 10855.3304829143 12173.6927806916 11560.5026848323 20827.1382691847 19516.2842922923 522670.739620498 21006.6433464389 13992.2055825729 6845.85555159086 14185.5068623838 13378.235518315 4532.4935520871 10101.5094663061 4482.41401189142 5271.41081496453 4825.57908582599 4926.34203848931 2545.99342160931 2669.68912949139 2556.00065257627 3154.19605178099 1245.5361151929 937.144661078594 321.635705503052 50.3823260739763 0 86.9436663955859 18243.48 121796.16 DEV 583.684525947654 1288.96557705847 2339.06228875176 376.19883551439 1037.3607135148 932.47709635737 1426.89407498189 1544.24328717769 1472.58121119227 26693.8652914122 2142.51667707773 1695.47708029536 655.999379978085 1452.89065090275 2199.38992948934 398.61310050455 1289.68092116698 405.677814074708 375.115479995149 967.179583687206 401.541051289532 265.777802088859 340.611052235899 266.896481194116 330.269600208211 158.554502328788 177.980356858699 207.125027141832 17.3933101264398 0 6.77804097078102 165.43908123536 3091.70341307183 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #