Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-47_preproc.map (3418106 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7023.96144812707 8651.41124561968 9177.55934140787 25453.5072201278 78857.0032964315 19090.9799422307 22082.7882331558 39759.6059207649 39681.4468664351 896131.115681254 39834.9961357761 33306.4235237013 25133.1546889426 40600.7882019192 24462.9390066241 78796.345053408 17453.1731443753 123727.441768198 16123.0034309536 29837.5325356802 13718.3539109883 9300.6586898454 8897.84132561972 8802.1639934751 8380.15313806657 4891.67864452247 5214.30675057194 2430.29861224285 256.864741773643 0 30.260617336304 31411 231507 2 6850.19497315502 8371.33625977537 7205.39364960484 22851.9313380178 73852.6915598554 22601.4060741842 19317.4868526848 30856.9636944678 32259.6952765252 919259.643480757 45296.0182586695 29913.2186081278 21395.0694987297 33372.7417070311 19194.1256790429 82820.6657966787 14489.4090464306 124194.374013756 22225.0280316495 23290.1784349638 14118.1500301181 9295.42234099171 12163.2605521262 9093.90076533234 9661.33758593765 4472.13050124809 4575.14727095386 5080.41007486017 245.56084479442 0 30.6542271941869 31382 229114 3 6520.92199502273 8283.89917853977 8142.34397249077 22241.7087453644 77699.4983043626 15466.141868504 22261.2289034433 46765.1926027527 33444.5974260178 955437.488788369 31330.5254068589 29971.4646769458 27494.3789720056 39782.2107974062 22233.6253870692 75255.1506770118 14442.5446173313 123858.374140015 15288.2834721806 24392.3316718566 14371.4699474759 10071.7944357131 10836.3509291347 5867.93126688447 6855.9820034325 5059.47810809659 5442.79381852681 2770.69342383952 162.126319587897 0 32.1424962299367 31646 238324 4 6890.01743497603 8462.71220240225 9347.48265444482 21906.4662717299 78596.0786575595 21773.8423401988 19747.603475812 35056.0879141922 38193.6729015868 923108.486819717 47640.1883079741 32784.0298202385 26712.5801410053 43926.4110277255 22276.1939119617 84304.8621411653 16915.3190442324 123119.541942279 15415.8416181156 21885.1045596249 13232.666383909 9158.03826386864 11629.537287761 6274.07966126148 7392.31090209842 6561.85407290687 5466.81883333354 4121.50178893508 205.405132394327 0 30.4056822744079 31891 228505 5 6521.76076794694 8717.5516377033 7429.46816376092 18104.0904009064 75869.4734067509 17950.6662987073 20352.4522051871 36354.0331625959 34132.623945081 990901.732097931 34322.5170803616 28075.4269235886 24293.6755339194 37465.4770588532 23629.5118770731 75165.3630668257 18577.8956602156 139559.413788301 16833.7934753698 19522.6208494907 15905.9195961072 11300.4059432297 10959.8884325495 7515.15611913806 7419.12096186878 5133.70125512492 3897.95512831165 3211.05038567497 264.203718091757 0 30.7685214106841 31446 234771 6 6967.49561766627 8019.02502185919 8288.97125611532 25512.3737965892 68832.1639852218 19362.0848962763 19822.6169527874 36953.4063481704 36800.4935338198 969846.830949695 43791.2963561543 28305.1699358099 24891.0149808141 34956.0165415349 21926.9350188315 79381.9747313006 18528.7045213785 130789.849509482 16201.9232650366 24539.4381907456 15593.8578504942 10919.5921964508 12150.5683413779 7710.06192297059 6635.96661577593 5157.71739210866 4908.99131272817 1819.39154154246 361.220798830836 0 32.0235672648991 31211 232843 7 6762.34460382781 8654.0267136862 9978.1470648672 27771.355477039 79895.4489151063 16981.582261275 18984.2372309126 39837.6005963489 34835.911872252 995378.334761771 28571.9457933218 29068.8832118573 22242.5858217028 41386.8038556346 24435.6738723843 78960.5561381018 18738.9083428959 121300.270511348 12487.3065860555 20381.3558635433 12750.2911504869 10079.4914322354 10828.4690514612 7702.63732791747 7821.29018259992 6139.43476053828 6819.79578651971 3606.07480291719 289.748214649374 0 33.6492952208424 31754 246334 8 6847.13930258036 9070.01744498368 10128.6588239049 16082.1342494701 79303.3011332192 21155.534435039 19347.6915066951 32206.4502577995 33580.9510352426 1042933.64092708 33217.2093522399 29842.8222776558 23944.9244529691 38166.0810361695 26603.1948282227 82630.585383083 18719.2359431097 119000.664706724 15416.4443995077 25487.6405798704 14142.5203431228 11746.1655925882 10916.1599229137 9541.04243434136 7797.97909037383 7700.98687089023 3552.93421350297 3141.23035857454 218.151708501393 0 35.7798430368644 31842 250678 9 6679.66974252149 8145.27224905249 8644.34060671763 20273.3184941811 77571.8471620839 23858.4104277647 19966.6297748242 32336.3967716625 34173.0185277172 976958.753750737 30566.9691976597 33011.1455007028 25969.4213746989 34038.3565668609 18881.4300289503 68793.6775331283 16827.9300194513 132190.426349032 15785.0032023606 27768.8270524021 14159.554161505 11544.3545112663 11954.0564593411 6970.49649580059 7675.43514870696 5052.66927614841 6970.85439185823 2066.65236465957 185.441146608079 0 32.2478472229524 31516 234173 10 6722.32000735379 8750.84680162104 10097.767806878 18544.5920167827 80053.4254267475 17624.1854269799 21777.0179657485 37987.6298649934 33145.1141857068 1018511.05213193 41014.0040621789 29881.9488861194 23395.402853437 37694.624303637 22039.0618664806 81147.3168896565 24521.1408466078 122211.149385955 15704.2475428176 28417.6527075997 13412.1057367868 10486.4095704961 9597.84639468054 7178.91359680501 6916.1203081628 7144.21607452264 5026.16207053027 3263.87147686857 212.363652833776 0 34.4081084630057 31480 239600 11 6609.1545315726 7729.89743201369 10660.8086541871 22455.4378198508 76467.6723302023 18011.6048641864 20930.9682514083 43105.2831147929 34070.6493091559 934151.452461606 34816.5459597106 33879.2210677128 22914.400835881 42843.7992493599 22372.4387164774 74651.8952832988 15361.2476366128 103002.499794501 12853.7862509683 24499.1874946421 11340.8328901102 9203.97290026712 9089.45754105902 5657.98819382996 8172.84828215713 4388.65589360952 6446.83794351811 3427.64914194084 256.8778208105 0 35.7852052149573 31453 235318 12 6790.06463310492 7790.5322705068 8652.65401004058 23527.770231193 88496.5777497918 21549.8639233029 18856.0939455396 31696.4842790127 31008.8934653303 995544.486833326 37826.5428103291 27025.8676239125 23681.4514892373 35443.6672420078 18443.6960539308 79363.819151412 15311.1659236439 130326.977056792 18480.7166995121 18522.7957925485 13129.3521786584 10525.3284166276 10447.3275131845 8445.78535145161 6898.25367106758 5130.01554354676 6575.1779364303 4013.67626816958 290.89358109954 0 31.1351792012875 31158 221491 13 7076.13392898071 7485.60160659309 10468.9942187028 14186.8842667135 75549.2817334448 21027.7433405105 19645.2137485597 30218.6326198027 34711.8330639962 927489.526263 35796.145512288 29525.7452651309 26957.7503230096 33176.0564687564 21108.9994212989 71692.4213972605 15067.7368204765 113064.342352226 17341.3882169518 24816.6695365058 13525.8231037453 10536.4782776279 10028.134152653 9952.985379294 7115.91361431172 4686.13543985794 6479.07875925829 2576.51562816435 257.431694443297 0 34.1682575085097 31649 245480 14 6970.6172706525 8350.45852446718 9861.51360885941 18705.4891313724 76129.6853773565 21008.252115102 21125.1540899139 37592.6251238871 34839.3294600239 958608.380889252 40491.9080080334 30817.5244117456 25111.2791426404 33955.3181478053 22598.8824161628 82961.2457423567 17385.4890874097 131069.246571855 14474.4762051155 23550.6315984681 14075.7234693297 11546.5908935206 9707.99293921439 9133.91220544742 6972.75441773426 4401.01395461645 7235.96121921245 3789.50525677709 216.37974974001 0 30.7388790580535 31213 246119 15 6525.37855989551 8396.67279597088 10971.7192552622 21117.9837772206 80256.9836824982 17614.194102131 20343.0150915481 33958.1124180405 32680.9292278176 917686.919003636 53171.5614091813 28705.9679792447 23015.9719560284 36417.7037747254 24574.9638146277 87546.621157217 16999.870817628 120007.603571489 17773.813615165 24250.0049762081 14039.5964140884 10023.7580698438 11191.3318110104 9031.64796591279 7584.27711631892 5829.14364508909 6001.61015780246 3314.73496705099 189.859433687662 0 30.2227007341124 31365 226023 16 6563.80117848217 9358.48258952436 8043.84410727431 18853.8253420805 87269.8841619696 21281.5059999477 18940.9485903153 36213.0965364516 30562.9007751977 947678.691010266 38405.4414894947 27573.9232548595 30762.0183269256 36666.2070853816 20542.2770007737 75853.3366516344 17864.8023045186 119202.235622759 13674.5184222422 21569.4597014168 13945.5635667526 10646.0589560451 10253.03509559 7731.03182523231 9553.16830908649 6199.46378665508 5770.00037348137 3899.25440592957 181.861913529784 0 31.896933104991 31036 228226 17 7055.74438297981 10048.1545768559 8380.73701755156 20616.0499410053 87964.3147123111 17395.6462937517 20443.0083133425 35236.4923792873 32187.6639392934 1021648.75818785 31741.887025341 28578.0874074726 25983.2792780409 45527.069300857 18742.7327714225 69512.8917746889 18960.1212288839 126884.963536268 13703.2966485312 17744.8509929235 13559.2184349017 11125.7854111218 10197.7098960927 12956.0407551427 8098.94339881382 7782.83565199146 7004.76919828852 4529.79175177261 235.442037177426 0 33.2677073313859 32102 247106 18 7160.44353926745 7893.95831940652 12570.9819290437 20297.0367562575 73918.2788384469 21167.2231990771 20536.9404874211 36357.9474886209 33329.1476671686 919308.661753699 31183.9341386414 27515.585491837 25766.3582065977 41227.611248222 23047.0072410604 79331.3025804032 15599.7616467104 127037.840264968 16346.7274442815 35025.295595515 12872.6419512285 12701.7809595067 10084.5472598139 8163.76351240894 7165.15883564406 6258.09300120591 6034.5001650843 4323.80959950481 350.489218594434 0 30.8379913425947 31586 237459 19 6309.02693705723 8037.803589302 10707.310619135 25127.4471146787 79368.7796923145 19864.3576249038 18392.5996988554 30151.6211361441 34647.0773745459 936568.591102385 37236.9851491132 28178.6879553786 24062.1944499282 37772.3253476333 21308.8233166559 77113.7041617253 17668.1711599462 121987.501822526 16004.7767027235 23521.764560372 13828.7531974154 10893.7262436235 9847.79295081002 7829.51007345673 8233.64152249504 7232.60779501917 4277.84536473663 2703.02431086786 324.182447326713 0 31.9321954555993 31473 236755 20 6963.70336043373 8318.12325645098 9775.85645621013 19982.3632500591 72985.6488454513 22070.2146055869 19383.2487551287 32652.0719145561 33344.0205515031 906058.600642015 30822.288400723 29153.5581920958 22048.7149079057 33314.4385544547 21092.6231102782 69082.5513112005 18305.2604614079 126973.181220461 14210.8654907534 20429.4959911708 14041.7094215835 9572.11859012151 9657.24742477799 7613.91200353206 8703.13524872877 6938.29677820295 8089.47618510815 5076.71733460958 283.315817389762 0 31.0740659020716 31018 227718 21 6970.19267167919 7761.38051640094 7580.70938325567 22600.5045865853 79452.7572761363 19551.9661364832 18434.9164211173 34327.1545577848 35011.5322895084 994938.180478628 30294.7746862757 29020.6079628497 25612.8945061897 36594.320733699 24458.5357241713 64014.58754201 17029.4424298084 133040.327404502 12976.4021313426 26348.9703183427 13755.6656273906 10709.4411492027 11081.4747805368 7144.95651504552 7740.29628732472 5384.24267912288 6583.87823766881 3418.31766133942 280.322713457333 0 32.6763016489559 31821 245402 22 6517.86405930507 9080.84621448674 12516.9798431677 21541.6401000747 81413.7132934883 20386.4474598229 19609.4140048593 35119.1929249459 32786.2027510012 959869.918232097 37575.5160162871 31419.4444212974 25707.779898423 41510.6193985632 20572.016084235 80898.6658282884 12328.9051611653 121705.880549623 15731.9607156901 23265.9655038617 12900.1669034561 9418.16025312538 10149.2788938864 7104.18251162554 7817.92323540521 5985.85435452117 4294.52205245654 2049.3368919109 294.170723418383 0 32.1299018061531 31385 233192 23 6208.81409107743 8452.32868566735 9123.04936761782 21730.0371713103 82927.0732402443 17917.5486471973 20272.2128910119 38649.4027548341 32964.6509024757 962776.307903633 52614.4597237956 26388.8226598962 25340.5937413581 35695.8853633253 20740.9630814522 69112.7339507042 16676.5946483322 117532.892145985 13403.5316056603 26810.5723046093 13549.5829194451 11918.0413623651 11348.9319312232 7574.50537544724 8729.2534303489 8076.60940737468 5458.34128980278 5146.60459610565 237.32521162289 0 33.3949783931783 31763 247639 24 6791.89076389116 8064.54555654081 8596.83452000758 19264.3677103554 73118.3335910898 21879.0594907749 19610.5965221501 34909.3061284943 30346.6377818778 987470.264956301 46237.2555047036 27881.8357499058 27125.4281784283 35265.5116013316 23036.3502944707 79656.3808516845 18007.5201047052 129737.33453072 16230.7803470432 24722.0903550126 13431.0657490628 10250.3626836851 10367.5837868433 9399.32781478519 7480.49014390734 6982.26426010011 4073.94799947034 1902.12783052878 212.139250034094 0 32.4092714280311 31744 234987 25 6763.5575940356 8167.98216762504 8583.83483172281 21789.7503197964 82142.5982129614 16884.9214130218 19804.9325827589 32010.5652329126 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107521.802302006 16299.0102054111 19769.4685239671 14032.3607290994 9320.17619445785 11606.2196335855 7820.25945380895 7698.14876278672 7861.52067037723 4108.55178034883 4719.00346396039 215.919976216659 0 36.7478760449782 31963 246210 96 7115.66588457331 8119.1992094863 8515.78334841437 19731.7861437407 88128.5752801149 19234.3938272052 18434.8929840798 39020.9629103836 36810.1131379982 1021151.72926459 28098.9322471189 28682.7265994904 26555.5834065989 29567.1271908276 19917.178393595 69464.7663765058 16040.4885720075 122191.634173581 14568.7001616069 25504.0211981445 13695.9425308138 9186.88118578481 10580.4997525257 7472.2466874078 7403.78861951685 5990.95436427081 6004.43442686794 4200.28085188099 261.797013354443 0 34.2782168210946 31143 231363 97 6358.44299072653 8464.8149473035 9997.88458291849 20399.8863626712 82844.0265107572 23673.0897961841 23198.1861077357 37963.9263692671 36166.1217741669 952932.588844476 42349.8209048721 29663.1881854921 27422.9860077216 36473.7687840845 23622.7547858405 76472.0136620218 13101.1166816051 116419.932559188 15684.5307418931 23409.9837906319 14267.5017090499 9944.47157024824 9218.52330055367 7702.24642306393 6235.87160569844 5232.01163124592 5770.18339558199 3188.6474801106 260.80584214538 0 33.2147062846688 31236 235860 98 7001.80457454397 8299.17482191192 8280.39457727653 20519.7606449325 69591.6678088317 19424.1096614361 20370.0330013164 36840.8813825521 34548.5171456106 985599.384132662 37073.7987320875 29152.0282870733 23778.0267252951 40399.5109990423 18895.3609273231 67729.3355718224 18264.2653857991 118431.238768136 14781.2259743638 21054.914541819 13129.5851849801 10153.7685816913 11703.475083164 7290.50054378883 7973.57768331095 4914.24939989182 6306.6156243418 1576.96469033072 143.760573711638 0 36.0150017148215 31208 237778 99 6175.06998932557 7205.27626959118 9932.55504653404 24779.7055692494 86041.8953253368 13815.3437411879 20388.4465888368 32669.3166429834 32729.4841562091 893347.926304665 37442.8291158393 26956.335530988 24092.9618662072 39164.8124254195 20946.3474347394 68299.9410671293 20368.9632908636 111979.607301697 16101.3602316686 26660.5348558897 13533.9250144809 11585.438170568 9487.91617606351 7024.38416769679 7543.02118918455 7115.01700365331 3739.57900754657 2318.7607696608 330.192975007419 0 32.1441528950567 31086 230387 100 6995.16753824076 9140.94918120605 10372.0128483535 24152.4518390053 81165.2254291822 15764.1898745891 21687.0641380479 35164.4726703828 31785.6950805695 914451.030668581 43551.2030059645 33763.8348559122 22221.414225311 40997.8849089655 20678.6793347683 74139.7998183149 15699.9854579836 106411.72131107 13323.5979086093 29296.5241561431 13249.2420155774 9606.51800953159 9519.29609742667 7821.46288633344 7984.54634518087 6390.21125397357 2726.83827222441 4153.40360767585 243.823311033622 0 33.930496101473 31770 235779 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 6772.90558734616 8340.11286833236 9506.04720080904 21059.4850157269 80046.0004127865 19552.6935002083 20415.2770500114 35988.3220110809 34008.9869721093 958670.936955792 36491.0958952225 29453.9007251525 25850.0212140657 38519.4442339738 20892.2804711319 76872.79529607 17263.9942100403 122983.54812636 15366.1606298041 23708.1469139468 13767.3518421341 10364.6686579864 10556.2688601558 8068.86411628644 7841.14868186743 5860.03192046869 5631.52809223289 3553.47458653852 271.47844457679 0 32.3080640396887 31502.45 236471.39 DEV 363.427188190073 550.705126086895 1302.64830183005 2689.77787396793 4900.77927365934 2862.59311913462 1533.3797801994 3270.3121423226 2401.11603636791 41751.3194030714 5806.0204678406 2155.31299325567 2685.37212876068 3658.85279492962 2430.99480645107 6159.16499745233 2299.30356167888 9049.89845325484 1946.88031861737 4013.40946133499 905.984098914396 1072.01676421958 948.266879052569 1355.2011213012 695.886460716495 1038.11601373935 1266.45104883216 1077.07049767003 63.8837424817269 0 2.25204899779656 295.611040896648 7262.84434074557 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-48_preproc.map (1981064 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 5870.90180983316 9454.08473481914 8209.76120840471 19713.7134606962 12959.6732124058 12462.8956516555 67461.3116468218 35670.7657330081 36538.0235280378 911881.726228385 111318.905253213 41226.5102628569 48063.3483482049 22390.3251691237 22750.1052248906 19161.2780814899 19975.725019611 48861.2970780639 12267.089141723 22509.5403214321 33477.3481745471 10194.543651297 5492.34518704628 5515.32880885901 4944.74225890091 7774.93991237182 9789.43966247179 1369.94616679325 232.566283898182 0 35.4049761873048 26152 230887 2 5445.35062343375 9467.0949525345 8492.18515359154 15484.4117379032 14481.946380837 15924.0796226015 39791.5161373189 28132.3757357059 36035.5547826196 922789.712180346 103653.53643595 33959.5775514187 52816.7959064721 23524.4388995608 29677.8330430801 21156.5109795614 24963.1236914362 47234.1523805342 15064.2662597638 27119.081693147 38221.5892759206 10402.0638914423 5483.766880793 6743.82080934046 4251.05514830608 8719.93362020221 11120.9569942478 2154.73941772631 403.899754118639 0 41.3133148345566 25957 232785 3 5078.34328101278 9740.67943457652 8601.35985178286 16531.0725616385 13115.2947802425 12964.7350206086 40991.8809155074 27074.8655388679 66896.2984912507 1009632.66033113 109043.587479593 34874.3013290289 56428.7834208734 19628.1824649725 25643.1838103375 20036.2406855304 21702.3219090505 46798.4758094957 13633.4460844545 22327.0780524841 33393.0496621809 10404.1241584817 5809.9594625957 4069.04133319261 5089.40970733121 8353.01973119352 7241.15469122597 1865.1744424471 341.56642225569 0 39.6267283213253 25771 230501 4 6272.2189271483 10583.3240187813 8074.922945126 15142.5577879984 14844.9849296311 15305.3519302252 66350.5890985027 37372.4954616322 61949.7149703917 1022239.16592789 87123.8800482017 22868.6296144695 57125.4406989424 21084.6676051167 21712.5551236254 20505.1803571424 23370.6277369992 46112.5451722769 14548.4688494584 21702.4477481724 35044.8018357876 11049.6459698727 6057.37503779752 5158.43536116262 4960.05696625133 10013.6684774349 11857.6769036705 1923.79871329481 147.372299766325 0 44.6461374663731 25918 233366 5 6337.15391890977 9877.54541735918 10514.0805776371 16350.0600955733 17119.9353635015 14346.6234094754 124236.805790614 56131.9174003953 118487.143021843 924252.585952142 79837.3533907571 33524.0791041958 53056.7391803783 21821.7423277523 22977.1486318751 18700.2875677805 21982.8993401964 47234.0888174575 15732.7310029865 20948.3412285209 35878.0258513696 9914.70343245548 4942.73219949477 3809.64597953495 4316.28251529964 10175.9279238903 13126.3624694527 1834.28577442364 197.904325432641 0 25.9273978550098 25695 226101 6 4913.04379691055 9883.49383474245 9690.29627800872 17732.6582045139 19752.4578105392 16285.4119068579 94071.0167507234 44932.1991877181 114868.485192943 1078613.38198681 151371.606378271 42601.9864875945 59744.2774429668 22190.5776055491 22746.9366631956 19303.1095230676 23111.4054692864 48590.5979290143 14846.2780599365 25975.5440226362 32020.9200442273 10136.8883810421 4903.28385274226 3871.65328425975 4204.15023636867 7147.45683417676 8612.92987616097 1629.6757042939 190.22431523829 0 26.9988163857722 25761 227870 7 6557.1295549371 10897.5906729083 9572.05320340044 13628.91601946 15460.5748277243 12173.2637259544 37528.8208124005 25659.3156245811 35228.7875623623 915759.71272247 62269.9481394195 25500.2839551665 49171.6197473731 23651.7727620687 24311.6582124888 20051.3254409162 24110.1845423429 43357.2180838281 15341.3582103808 21397.8376957351 28469.6161682373 9380.71016645354 4808.39181760575 5119.25573022499 4262.41299812003 9087.52947179112 8960.79192105877 2444.42898951035 177.164129116228 0 62.4248715738658 26089 220657 8 5680.31678112519 9135.31844137558 8989.88145068161 14998.0903598331 15822.5101644204 13702.2991454455 122499.725944819 53773.9049805572 117983.524924489 1031758.54119498 81988.1141460915 22484.9335103839 49431.7777676105 19308.6714507812 23183.3734313798 18622.5645102668 21540.083042767 49732.1908394038 17049.0038775348 22514.7099730384 34290.1461414644 10272.8150764353 5544.76942331627 3609.66287338656 4666.40193152749 8337.33797113808 9178.27849423015 1494.10967604841 238.87487162707 0 28.9965090076136 25739 225008 9 6864.2750402303 9843.3463041962 9528.48949139212 13221.3059646913 13841.2522871601 17082.0037717946 121548.846274853 53967.3179124925 91882.244100361 908740.524085682 105668.403839692 44890.5539973142 53220.6581218444 22156.196637293 22596.0282427569 21983.9124540761 21753.670239728 47785.5061685448 14432.149856087 23333.8270169939 33022.0306859035 10262.7166689985 5567.26570686764 3306.1583393259 4357.31908485303 6999.71751615105 8939.81837752108 1852.29815652625 183.85469824199 0 26.1529759501241 25501 224189 10 5171.26001427349 10290.6537479572 8953.95925791322 12497.4858152757 14600.670509668 15607.3674371208 40662.3676262306 25237.9128845804 59403.2821668524 962494.210841209 93372.7018657317 32735.4226692912 56352.759552987 21059.1491736243 19083.3250884629 21615.5695781906 22156.0586690577 46542.8161201698 17500.5273533668 21321.3241946845 31055.0647677355 9531.5300220297 6509.64388495687 3623.40436062539 4638.61887847249 11157.985444987 6779.17967005946 2327.33306961777 243.59406063792 0 43.5948646050943 25665 232646 11 5912.07723952604 9506.19919351591 11898.477628816 14802.2838418121 15680.0830446488 13092.8012854118 37473.2563582237 28236.4335892794 117937.415960171 1154267.39389492 173993.092329095 42660.2535247803 56948.6761515335 22753.3553011977 21743.7158998832 18840.307032988 21588.4207392619 46194.6851010392 18469.3333329159 22421.3475443319 41196.8448812251 10290.4291325594 5214.80763046065 3539.60703360552 4415.31600706757 8419.91757498207 9715.35149089317 2485.35662915141 198.010548702332 0 27.5135980735421 25575 226158 12 6356.30724818271 10374.3347713465 8868.92867783011 14593.2255942491 15142.3712932604 17036.8349945082 95777.3519584456 45000.8072869152 65936.638047536 944824.240857155 79873.2536873395 36449.8127412401 61619.827536254 23882.6033166241 26860.2590644838 20525.5280379598 21813.3299845325 44858.7834279237 12641.112553885 21989.7419622977 42565.1311655581 9374.41192157713 6674.69995188039 4953.50265831008 5069.78682540294 9677.05533345678 8682.07847491673 2117.31447033674 265.947575605167 0 36.2889485816308 25819 230197 13 4958.6640420863 9088.31759364381 9215.35863917086 15225.2554966818 15630.6513165961 16130.2795252359 67150.1697354412 34650.3614319632 91321.5002705257 942569.477466463 143158.201871109 52631.5917545073 51741.8265840272 20060.9599951502 21332.1832438341 19577.7212011746 20769.8206999186 45703.444218338 16414.0074116891 21050.4117724072 36312.6013524847 10738.2014074439 5584.23830120219 4057.50583766944 5607.08118951635 11212.8110547682 9750.56648086186 1937.16307968722 133.251141311765 0 27.0541770355446 25574 232976 14 5393.37105590213 10711.5178537711 8722.89026340782 13750.635158654 13545.126254464 14614.6462068499 40490.2335129747 23629.2563234447 142131.748424978 937978.293510847 107406.046415713 47308.8760170411 54769.5942841701 21144.8499055949 22999.4132900862 20219.0666053138 21386.5073330998 49123.865503108 15871.3480159205 18227.0522766455 31480.1492815497 9399.79352655299 5177.14694877 5540.97354186017 5028.02041031419 9067.10673087774 7803.22297228023 2363.78911809429 127.888200376126 0 26.270689358247 25922 237643 15 5576.06542777487 13168.0903245556 7163.20964203796 14716.9679630995 14085.3873104175 17149.5143793036 41144.06353985 26507.1570114018 67864.763538336 922545.213830546 93225.7514648912 30937.0418429397 52922.9749155214 23054.1055113468 28090.3188128333 17598.4339540034 22416.231123272 43204.2317743777 13640.6397780876 19635.2520193373 32033.5271926314 10790.4490896357 6494.96750546555 3940.06752441986 4428.60377401998 7748.46980470946 9592.48938844628 2144.65070380785 296.709485348256 0 41.1833958772019 26537 245085 16 5989.25792533842 7212.21201489936 10016.0704591034 16930.5256325276 14851.3928522226 14717.9375484076 94456.221684859 41789.8572501046 34074.7069793394 998111.430743058 97860.1297056544 37388.7916397575 52478.1263561917 20899.9769495424 20786.324267528 19841.8736312615 22703.8012988507 40050.5963548734 16484.685763127 22771.8664035932 39972.303342995 9957.07355120004 5023.80675027985 4696.88733924374 3861.71432825557 6558.35547575011 7269.82195007446 1557.33488629774 288.556605816605 0 38.0981875420039 25693 217015 17 6145.0453278254 8454.42506988006 6907.41409729033 17155.0588252975 15220.9607803537 15038.1828971019 90815.9197213383 44510.306626322 42056.2554753016 973585.753302678 70333.1204589257 25453.4476656481 48556.1305585386 18967.0855250972 24564.9730564353 19117.4298404461 22086.879017601 45737.0249971979 24100.6702882358 22243.7882806562 31917.194865514 9801.52227715601 4956.2406835539 5939.12104396399 4361.03043351503 9371.24722248807 7144.43464110881 1346.22309918775 227.370164962894 0 43.6169725139263 25628 223503 18 5986.25697961602 12915.5151144223 10241.5646836004 12499.0281008763 15182.5941848149 15155.558133527 35837.8797332986 23729.9681145524 37416.1222294527 960063.175998228 101619.986803023 34689.8139294845 52523.9964423897 21109.2567828984 21804.3685981019 17669.4224306193 21070.5430144841 40401.0997369673 15626.8232986756 23255.9683443033 33656.8981135508 11276.8549066818 6083.49717111855 4419.30373094003 4839.81779091756 9012.10299272981 8630.18567637078 1626.45597404943 152.257742369634 0 44.3822572585113 25799 219826 19 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3929.67482481304 9352.22220711082 2555.22346953181 269.92160140779 0 47.1585263672111 25987 214614 80 5423.08637068683 10555.6140103615 8790.15899658004 18731.3999737655 15633.5847643932 14527.7972854425 40472.5855354964 24377.426843566 34821.4918829935 1014229.54432989 144572.03974183 43942.0191229043 58736.7738850009 21771.8492037293 26271.1031128098 17913.0531894133 23591.5890487095 43864.6684993603 14883.6870837286 20003.5177605478 38049.411717961 8867.26035884063 5521.87657850764 4046.45523463035 4045.84351364005 7839.43563501247 10220.1335361334 1866.34021963717 352.651142368491 0 32.7871191832201 26139 231493 81 6315.04827456266 11209.7659830754 8475.77582027181 15291.6992779905 12337.3158363593 16614.1864300216 65254.8029447424 35511.1588810573 117468.613646482 916273.996660808 106166.769065878 48947.8694215541 51959.7079504303 22182.8367848814 20878.2324298671 17230.2443170221 21567.7467520677 45644.7669208401 15111.7113803782 19142.969998155 31876.4649034166 7988.11304231486 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24390.636184243 31186.0225172413 9711.28059005523 6072.86160891256 5292.15752697309 4486.56651363544 11212.6746763419 7909.43003074073 1537.26672138372 183.202563158428 0 28.1454997843793 25726 225028 84 7411.5481246035 9691.93243573879 9208.11683667369 16828.5881780606 14345.5036279701 14808.3783642419 66910.5207387586 34107.3956738479 62203.3786173398 1020228.02930319 107507.208231838 34224.4293343341 56063.7341162951 19189.0087009445 20495.5727451977 16994.1563617285 21875.2882254311 42988.6391367192 14712.6460695046 20919.2381265723 35723.6053308756 7787.05698481201 5728.68522228836 4210.1214442816 4410.68937002314 10907.8738848958 9361.00918666077 1966.71085399694 207.198545245813 0 39.309792495541 25665 230006 85 6566.85770700359 10389.2027475963 7714.41361198423 15587.0945485059 14961.5609750304 14449.366552017 65976.6669980905 37555.242917805 35958.5749484123 890611.793554557 44483.1028949495 23530.5645507663 47738.8928935249 20677.5999248551 21887.8260537271 21705.6583771823 22378.1384988486 43133.5127360569 17779.7305126998 21305.7637146847 36391.3891169196 9243.2042028258 5343.71576069275 7032.14436091972 4079.23370349729 8906.3743065595 7585.78524478728 1952.07950176062 133.90580083312 0 56.6210246279826 25878 219646 86 6661.72888400242 7294.88547092692 8137.56977940645 15012.8377066635 13680.0015875756 12134.2094533588 36287.3313459138 24307.4077992287 62129.3914893722 903603.294191543 73328.6471309787 32946.6027449785 49930.4075384617 19239.392934031 22698.1038530601 20418.1569378763 22739.9160344288 42843.6645101131 13579.506041937 22085.0725028587 32387.0234531697 9347.91524495078 4681.97217733927 4524.18451204112 4650.26068693517 8748.93431684091 4644.66039038115 1876.82992908596 159.923702384087 0 48.0577462160961 25927 231025 87 5604.94536528 7103.89927112294 7265.05805899311 17841.1423943597 15164.5803667764 13778.1791413802 173628.636980431 71502.3163779375 141476.837034329 949698.010803893 49934.8649139657 24917.9705164625 49210.5686551921 19594.7457307777 22707.4403051379 19574.8133676224 22022.85911525 44936.4925358638 14540.3204744553 22015.9581571633 39377.6895354256 9801.82938957177 5663.05848739786 3439.27770145316 4471.52928132945 7070.71290895871 8225.14426892119 1370.63321759821 430.178865513214 0 20.5999773472353 25265 214970 88 6656.58178588888 10721.5043433877 9345.07737359283 14615.8243885219 11944.3366351065 18151.565106562 67248.760609475 34965.8546735129 68859.5068424414 983454.200897041 132656.654781978 41173.2378747786 58452.4233952776 22611.685940683 24539.0980571107 21274.2170929515 24144.4386854692 46594.4152122494 14627.0654079326 25300.4640874601 32726.2978091238 10115.3525641691 6197.14905499256 5705.28389474165 4055.91144036607 7884.76381420783 9878.50692524417 1938.20212019705 100.240840197389 0 31.9163032838112 25798 223914 89 5908.81041749657 11419.0357861401 7629.33525229335 16193.6353974796 12244.1288661232 16201.3151884819 39085.6236783674 27536.7527857117 33565.428847272 974917.941648225 112246.022750524 34311.9335537082 53299.5836201428 20637.9534414648 20511.6127711304 18170.6332159539 19997.4385498193 42882.5436540788 14764.995524851 22439.5799684406 38831.2753807399 10308.4714075482 4730.17852946916 3529.7399947242 5142.19976368915 10036.5926918971 6339.88724956949 2100.17680770551 138.14954284318 0 40.6089950084371 25886 220326 90 5350.56794332965 10992.9745076362 7570.21687526407 17427.6503332347 14078.373438046 10936.6504812944 67914.4880939374 35722.6529666309 89488.4826657316 891889.623188383 75268.8617395614 34988.5528282131 52808.2357636204 24922.9621240219 23787.0437306876 19617.4790941583 23970.4610241563 46052.1354980132 14291.3844682122 23274.1924011513 34289.6644709524 9554.72738092662 4624.89252529756 4062.53351836297 5026.17167597579 7367.88931659435 10075.3603467834 2340.00338462784 280.69705023439 0 36.4852841575223 25778 232421 91 7069.70286627957 11418.1261241027 9783.45824668366 13252.0944349848 12719.6243571081 12327.5780801787 68454.6771859171 33290.2090527125 36468.1966834915 916420.404290219 135852.869112942 53610.9106674113 50790.3548226986 24048.90663968 24122.4643239062 22195.1236898974 17987.4758971881 45636.8670152734 16422.8921978212 19973.7333254288 35119.8873960185 10695.2536496753 6281.03313554269 5230.92675836267 5142.09960153215 11757.2777879056 8955.38158702429 2091.36889850641 325.815345864852 0 29.914340939121 25936 227120 92 6294.4587108304 10411.0157856253 9949.94660437257 14109.7473790932 15296.385546912 15589.5001815657 67102.8815067744 36613.504237212 94526.4343516432 995299.993429172 70607.7586512592 26662.678335701 51102.5703388483 24734.0743885115 20206.3433773114 20128.2013830546 20983.5724217529 47129.3648555032 14701.3586216611 21340.0257667541 37815.0322040702 10747.3567551066 5902.30702109244 4744.62564520219 5135.90526702619 10330.466125947 9717.00677383162 1606.56299558424 215.735137192564 0 40.824924827763 26111 229521 93 6306.38942474573 9813.88651508107 8144.98828181552 17143.4050422973 14800.945432816 13248.2720154051 68458.6686125511 33550.145697874 86771.7737462079 1098252.09560063 106433.759393084 23600.8533107194 50075.6190446789 22620.6237599059 23208.3603959457 21158.4150653355 22218.458080417 51438.3850220949 16714.5364434033 21167.8363238709 35698.0102545684 8056.89369498596 5401.84909557184 5143.99061296697 5223.41849475144 12748.8230512228 8016.9786370611 1663.92588257346 201.983413648757 0 39.3162756665147 26130 238184 94 5505.71507781817 8404.79567978513 8518.18331249844 15346.4365515798 13830.7466270737 11367.254239313 63023.3315530625 34519.918254976 61566.1385481 998566.061863022 124326.177402094 43098.1143317282 54884.5970601826 23800.6032246016 25000.9021517295 20501.0621981221 21023.9555151552 46396.6935771088 15291.2025513948 20022.770832493 33449.1063639445 10085.1673271819 6224.9487627554 3681.5916160734 4773.10958402396 9743.68075389761 6834.67060886036 2007.56435503473 236.35405061781 0 34.5551209626041 25448 215992 95 5895.43808883171 7693.71971017443 8806.05399886732 10319.5834944644 13771.9694383019 15194.3622412857 64981.0304408417 33244.1058608687 37581.5345259698 955816.502047063 70005.6939624886 24068.9892062129 47399.7371645525 21088.2577728873 17869.5809134512 19441.4521969309 23032.1786381 44926.0164394312 16994.4352322131 20318.4153902852 33225.6203630031 9430.46099938937 6005.90819548034 7023.46762570608 3914.44011000393 11222.2338448377 11608.6733417139 1202.9412683067 247.377425603526 0 51.1959716067887 25724 213774 96 6284.84062828619 9318.37756412978 7485.16517589321 13986.8432635766 15512.3126562196 11652.7061699812 41091.1175774327 27888.4413965635 36812.1179921463 969031.668092704 103383.664965495 34734.6049244302 54845.1280973456 24445.1961332193 20086.529857875 20311.5963038328 23172.4553793856 46237.8221214407 16105.1940831772 22997.8888866217 34771.6844324848 8931.48886890929 5530.27042746432 3743.46836824902 4650.37705982832 9430.81040519325 7775.7713766958 2562.02911069387 95.0169369101664 0 42.72076872099 26115 228739 97 6374.6367530772 9395.1834493029 7294.27857955197 13707.1515293543 13685.7752534685 13428.0422177176 38528.5534649794 26859.4420406689 142773.594537924 944068.737537775 120351.338735886 42766.6169914278 47571.5020991166 20153.8481249415 20889.9000689508 17865.2728127663 22441.4201058975 43206.9927254671 14302.0276142606 21270.367861934 30045.7343466867 9318.66036503777 5058.59709443444 5049.93645221368 4629.24215022017 7666.65491883228 12482.0040745621 1460.85676017053 167.129700032237 0 25.4302868614461 25494 216486 98 4333.55738401081 8489.8482061972 10713.3835374925 15450.1874318596 13694.6625832649 15225.5075656128 40443.6859406245 26667.3242546221 38909.7669283202 941319.591274407 54406.5316606172 27163.0812494176 54897.7232661259 21027.7417504194 23247.3459260463 18761.219557862 21345.8944596329 46894.8256448575 18487.200865456 22299.8851240283 35936.7620484538 8955.47446361394 5699.8768809458 3654.51362963541 4661.22562400026 8196.4558061347 9108.90901693111 2374.41922657131 282.572120232953 0 63.0181004817134 25633 217920 99 7824.42464773853 7705.30393652079 8295.26239460297 17814.3107471567 14118.6547055152 13168.6254155614 41677.2456916066 24377.3074941918 92902.5751621165 983674.621250884 99544.8079999296 32532.039079579 53159.4113923606 23840.1074682989 23623.8216284333 24174.6647096645 21255.527666769 44980.0694550576 17280.3379797434 23319.0044429005 33804.8605221317 8545.98953859465 5375.7965262079 3609.00264607372 4506.33123253448 11374.5095725455 6639.89109816686 1836.91362123654 190.876368643937 0 37.5242947520228 25622 220439 100 4973.98667642833 9224.84959694238 8193.82777796308 16474.948439671 15766.4172761037 13044.7644355422 64506.1154978056 33036.1170964732 89525.5252514998 925039.177785519 80684.3407028077 37827.6993447723 49895.3059747667 22802.7421121761 22077.3853918225 20649.1858746589 22673.1346878788 47206.4034204002 15825.975849051 20457.6601043274 34888.6637730799 9016.70647628017 5050.80224502684 4894.53759394563 4007.99173598878 9913.12950197874 8969.33920408352 2082.78136661148 162.368048124922 0 37.5091552098893 26117 229291 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5865.83246987811 9629.31831043105 9129.61318692518 15826.1496868507 14451.5225732257 14257.2646138857 67378.1002047323 35140.1839932099 79430.6862335957 971537.292199752 95048.1990009366 34250.822184866 53981.7802639923 22149.3629179231 22739.7404114112 19720.2739663701 22032.1921964203 46202.8484834023 15623.7162308854 21791.8282237893 35176.1711542114 9767.00192522416 5468.6143161509 4569.89776048758 4563.82358770422 8882.20625237556 8771.92387991741 2010.78610246961 235.381992889325 0 37.1292993583688 25786 226285.06 DEV 709.474595733579 1281.35133950876 1150.66018727249 1786.46942818895 1310.86500762062 1686.73908546989 27404.7607244878 9536.37111939394 32147.0049720647 55494.6382019752 28092.9397631172 8405.96006751478 3724.2694651029 1680.97842672911 2002.34656919952 1327.36934115837 1541.80060175494 2868.31592320301 2045.64576737813 1693.31845772161 2955.24202249436 829.224833920737 477.518284320644 1005.18728540577 417.248422019794 1762.01129753502 1680.67974548731 367.133465640884 77.7674615564755 0 8.80042709064314 230.876027339349 8752.27982850183 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-50_preproc.map (2812767 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 4560.38334968854 4839.82275036523 7928.8036747238 6578.19655350444 13039.1298794906 10679.3932033483 14809.305709199 25276.8665930712 31655.2718306949 734310.462157769 23033.0073633484 34954.6343633484 15090.5950535645 17574.5215268345 15088.1446619046 45923.603110722 12058.0568630709 20739.4252808058 7040.80701709245 13446.1781187858 10322.7140100292 8389.78156462149 9919.82797048369 3863.7996005403 3853.40892085007 18192.2605815657 1705.795529431 1470.46352818362 430.173389564515 0 66.8015389395802 29112 219438 2 4329.70210472758 4176.89575261608 8194.94861170494 6578.36469916525 11845.3249251738 13163.9453014451 13590.0089246724 22147.3382040898 32571.6497084206 677885.09134454 19960.3372268942 33452.8256731407 12232.4384367624 21846.7465581619 15121.3607165447 28404.0086071155 13795.4821575517 24985.4157530444 6434.69591902674 12101.0380809469 9280.23500487441 8671.07817701831 8028.61190595801 6491.54149891707 3989.75289589268 15991.3469928902 2342.3609716363 1357.90091263407 532.78305672743 0 74.8691392627583 28936 217365 3 4942.88063065965 4132.68535645644 8156.60449729725 6741.86475220255 12679.1078667316 11107.7259418269 15315.1264026189 20825.2282768393 36369.9420268605 713349.582367812 28864.0363251486 29318.7955682131 12931.7026439879 18475.6663379604 17721.0284475631 29161.3365438654 13250.4014964689 25786.7967921864 7357.31285568729 12940.4807165975 9958.55361487468 8204.99500696813 8374.73558607038 5716.85610150347 3093.29277364029 15880.2255188423 2008.16281399148 1242.38056186419 525.590625460029 0 75.9631518041426 29801 228737 4 4356.06699729191 4789.71776868979 7856.62053694697 6302.39336564385 11829.0080491659 15875.5065870477 14793.2173200592 27607.7231037789 33007.330192993 728016.101304724 20573.4513765794 36669.8921404045 13528.4606160771 23651.9361317683 12821.573999445 37901.4206352916 13638.3522565349 24186.3964773876 9569.10514651271 18243.4681743171 9508.02796383414 6826.5409334849 10112.5400032719 4878.26872923844 6232.38578242571 16581.478126387 1854.70508002532 1896.63399971954 401.223331234291 0 70.5086905010674 29062 222746 5 4925.77688541585 4732.29693101737 8333.7255458699 5545.19785674432 10680.6132090266 17660.7259253577 12240.9178812416 24143.7530788924 24787.598168247 805152.738532874 27903.9758557016 32629.4640890395 17092.5573654331 24131.6736147374 12956.4355953001 28785.6931340312 13774.5981371761 28085.4849585423 8569.5490507007 11480.9414108508 11133.2369316033 8172.27807725078 12335.5695398923 5865.63500401446 2763.43342180249 15490.6796445464 2273.93802144212 1534.63030677264 593.925222953137 0 89.3178510480203 28555 205277 6 4883.65665662015 5184.52686216838 7031.12848881488 4875.67005142135 10805.8670665236 13074.1524879457 13984.2737257831 24422.7327681335 23139.7939486546 706562.154103271 30102.2613486441 28252.6784484621 13785.0060086447 19333.1009064611 20679.6407164955 19455.3003953171 11545.2108611348 27406.5036329896 7314.46561536879 11023.0565232462 9526.90262678964 6928.96399117629 9654.30411056076 3955.57862923575 2715.06817722727 16176.6569971374 1721.48130658872 1158.00920326586 302.712013416071 0 85.1147788676934 29098 226484 7 5430.52649320744 4198.94258724215 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8784.74933973378 8726.3685663555 13426.9560747607 6037.23931453416 2863.09249616 16079.4727345852 2030.6179660701 1396.19562582897 939.510620648477 0 76.7321792003896 28900 211258 72 3868.48731428814 5612.04782994023 8350.24007276576 7096.09154537322 13973.3463439832 16250.6022250039 12179.3699019049 25495.4936932624 22681.1242327768 671081.065827983 27887.8998840719 40526.3081910522 12278.4095874841 19783.4525413161 15258.5662216107 18280.8073427002 14596.74424636 23958.7664481918 9637.26057669757 11242.6307334552 8370.02426871087 6417.22834914643 8949.98341345748 5115.06260157621 2953.26539698283 13483.8915924388 1742.35701648179 1246.73750738393 216.661585096647 0 74.9480535987776 28720 230194 73 5403.84650597601 4510.55903550474 8986.14823334637 4752.4406596577 11819.3137224072 14660.5240389576 13472.237517357 21993.9447569279 22575.3781348844 764658.456015793 19331.2782611578 37116.2736828308 17312.9554595495 23877.1149617452 14113.7596170967 27981.300127745 14542.2574504728 25380.0839167352 7762.58959053629 14142.9636621987 9967.98173731197 8175.98891762615 11493.0241698453 3470.25127430016 3129.95609301039 15474.5278138995 2135.03477822505 912.190066751579 476.350538088133 0 88.7027828498324 29219 225291 74 4956.78916217782 4638.59524364723 8133.97662651678 7103.27177977635 13070.6301776822 15682.1454270447 14021.3491177968 20148.687450655 33528.9959273846 764206.554287399 22800.5032334037 30625.1420915056 13127.811717694 20923.149036919 17931.8984795986 10165.1385988329 13735.9367997756 22843.003254916 7938.13311069251 12366.026873444 9930.74304032077 7122.32800212022 10563.3580299185 5382.76905397718 4224.47702422234 15490.6117835908 2398.91568973668 1189.59366574784 382.404430304134 0 94.8434833704137 28932 213916 75 4680.62792814922 5022.98103627561 7659.22663331679 4618.9376902085 11078.7138532061 11289.5960702932 13257.5550287812 20264.9989348701 23047.2328972234 791859.267345425 48463.352016115 35018.7906212588 12831.7641204691 24046.8529834914 16793.828384647 38487.5150911188 13668.1794448124 28183.4147227905 8903.26586621216 11043.5568646221 9700.24996587317 7203.1438167196 10961.3603548242 5159.22094448586 3939.21356741503 14807.1577929365 2128.37673210163 1353.10035758724 547.462929918322 0 64.5413084195083 28966 213620 76 5334.47560569964 4905.25912149227 9699.18771005902 6788.98709681486 12472.4020862429 13902.1245867272 13338.5047783204 19614.5456431709 26224.6146818193 746427.894748183 38411.1543374306 30245.9250708014 11715.1722728966 21687.7557677643 18025.0089789287 37544.0535563504 13144.6728804143 23467.8405734621 8224.87613097136 12001.44533568 10055.9021184634 8057.01128745967 10364.1980902027 4418.28558769314 2676.56525056563 14930.1204411752 2076.83305571942 1477.36075088197 410.050342562627 0 74.0981279958009 29425 224151 77 3996.32995841298 4557.59102591599 7233.49790771746 5265.65400711081 11612.201391546 13067.5998516307 13621.5974376009 24157.9198129621 35904.7886586391 801936.268315275 29867.3356347934 29237.6942725964 14834.4030843381 19381.7745291654 17272.2463763782 18305.3185812994 14554.5462412336 20897.7192391974 7722.67904810267 21600.1095480618 9288.13201525243 8836.63384737761 12762.3011511645 5667.67735766254 2718.28135354769 14591.2680001444 2205.98187628856 1408.87871806308 257.822951225505 0 89.230327673814 28974 200230 78 5035.58431868761 5019.36385586684 8620.30852384402 7771.49941985854 13876.949572325 14038.6349475344 15177.7672514564 22915.5757985398 23076.6506999775 672934.138042622 20394.1374478714 34567.454263232 13655.8576344463 18482.8733286189 15002.2101934529 18275.2292275824 12465.1772510793 20945.4697810445 9716.17772808536 12880.4957725719 9975.30717680945 7808.84353028304 7936.61261970446 5155.02661255636 2700.9091762745 16263.0617956888 2414.32412904727 1172.94423063957 408.22517573051 0 93.9752867758187 28729 207060 79 3977.3328917632 4584.49873758726 8590.18632244584 5359.18631770733 11614.0092711465 13078.7668178967 14009.3949151254 24949.1084037687 42010.9674226914 640415.19956208 21117.8704889141 34777.0192095095 12363.6577645986 18524.8289437644 16963.6075838728 27467.1447093919 13939.8759042884 26313.7414561829 7834.37157729069 14093.2342473441 9158.8969425239 8317.92473706873 8032.74404724434 3992.68488335348 4657.48425405357 17394.8952292737 1895.69943160414 1585.41278021297 398.819739848782 0 61.9130195339615 29196 227172 80 4808.1524514411 4466.3601951676 8230.93153017742 8282.89136005243 12139.4496524825 15233.4005019881 12732.9851813255 21060.9547214893 25460.9863403663 779453.148690826 19133.5175840602 31103.4156168224 15912.919068574 18533.3187372945 16092.911094656 38103.2018879617 11423.5706150544 24794.5434998724 8178.104843011 11696.2176118502 9849.91662910991 6653.51321647794 11489.5560090311 3954.48404488783 4287.29398601992 17197.7946187707 1867.09928670172 1190.41362646111 583.140153079297 0 87.9876705710611 29142 229007 81 4671.52628135712 4649.10319237946 6842.19921164364 5781.01793472458 11144.0260840255 13618.535017854 12431.298948312 25164.4352118832 23158.3322129129 754241.915896588 28039.3260840548 29051.0634230616 10895.7080927347 17572.9171617892 21134.8152831502 27388.9840379965 11556.898638658 23261.6787952281 7496.81818331385 11879.331415642 9441.73060482431 7002.17993307439 9977.76814490267 6919.31794993026 2449.05890151301 14644.5649255676 2027.86026727996 1220.24297731467 634.819365627592 0 89.9709984334553 28485 190160 82 4961.93210550614 6321.85072595322 9503.02474752593 6893.50983661179 13041.5831018635 14313.5830483645 15037.6968560108 23324.6580778884 25147.5098133373 740682.111982262 19236.5461305343 33962.515899529 12211.6982690698 18413.8393592803 15349.4186189229 19376.342573716 15102.6272867016 24957.5125148643 8073.26755498923 10833.6175886335 10891.8376716632 7189.54093102825 10441.1701901312 6353.52542466609 4744.85967487293 16052.6170516549 2069.73649124969 1603.06864348235 339.089981896788 0 98.8242905467331 28909 220349 83 5890.79776938599 5710.01752985827 8765.48607624894 7530.65561797802 15378.2574950649 13087.9968333708 14149.3276935924 22846.7152130869 25873.3187598522 777810.99003162 18165.6160902837 37236.7254516748 16814.421776095 20663.5577636354 13303.9098186967 28273.8696526677 14664.8259536098 21176.1980133388 8758.53351168021 13260.7437010438 9831.4480130635 8235.61112595099 11048.6635251396 7724.48927608034 2500.80215523614 15505.5191769945 2687.22821152255 1308.64152394937 406.30360591548 0 95.3992397635236 29058 212880 84 3807.5927900426 5389.71931780548 9244.8394908461 5283.25020958753 13618.742532986 17638.4845700475 14825.9266558144 30118.5563657279 34181.57954471 812129.677034823 21910.0304797787 35124.7922060863 14235.4236903524 24427.3379152175 17405.5222619249 10457.6712433165 13581.6019542358 24895.2151758444 8793.95622181719 9926.64648641716 10322.9182732216 7973.03065913146 11980.1001894925 6202.52428813928 3384.24946024005 16217.5642053618 2420.97387424486 1065.78378045533 369.294315603605 0 85.7233618764923 28925 226512 85 4790.8123280917 4158.14015662012 7480.57026436147 5713.52613301587 10943.6360172782 14657.4668336254 12493.5442706353 23462.3614594543 26140.3148759892 762445.167134895 37367.6815699626 33263.5598554548 14150.2328717776 18764.6204053008 14909.6768886076 9530.27852800906 12888.6377645733 21561.1856477305 7965.27723125575 13605.8731133796 9304.32498356958 7210.10235243309 10738.2683156736 4234.5025599838 2552.36023073114 15544.4495757358 1912.24612946645 1298.81713673972 494.870758613917 0 82.5262429340784 28943 215509 86 4903.80553277934 4767.05884778437 8163.96267572869 4555.41075742241 13536.0208440741 14251.4701295118 13787.1467400677 23049.2081158953 34171.4841200342 788057.512970466 39834.0588873654 29185.6686188007 15939.8600934973 18886.914435324 14291.4195489976 9873.09624214803 12332.8480699217 25007.2304048758 7605.65808541849 16495.1436818163 9390.87084204946 9373.84680446962 11753.3893890479 6093.39424212998 4128.8605615528 14474.5296339681 2049.79700750143 1608.38973070567 389.019120824179 0 79.5222773937134 29102 207891 87 4757.36020492741 4058.6681809213 8472.9031905425 5314.87141613008 10582.0950139053 13887.1541483641 13041.3514019695 21369.2233871355 23811.8144172286 812401.842194212 28508.3209184154 30361.8657303678 14314.4832986808 21209.4825087212 17991.8347441305 9846.18166864796 13387.834845678 26088.1601918739 7636.52646020046 11856.2216866643 8865.45548330938 6951.05505630917 11692.6281649387 5340.26096602022 2631.29468173333 16601.2281786537 1888.98828204554 1450.66757794168 232.108883165982 0 100.90223087311 29189 218774 88 4527.5117182867 4973.18050523991 9580.19489412926 5786.12874489359 14603.2694732929 17542.9134708015 15940.110319342 22921.3854449574 35724.1082059041 822211.861823218 48592.1711589351 29837.2461975694 11882.9972535408 18092.2158688692 15585.3117239532 17671.932200581 14192.6086384065 27574.4011361628 7196.34839114737 10173.7386420947 10226.9597254059 6836.74428437867 12826.5400974934 4736.09088244485 2993.02433639137 15447.8428299213 1946.89866702629 1477.84219511831 543.186825198 0 72.7571937652268 29246 220857 89 4983.45052071357 3563.35073167858 8718.06365579165 5137.22219210729 10814.2329295613 14470.4284231475 12035.0666804276 24322.7868309819 41423.299871313 738260.873910811 28307.741100066 31645.6815400243 14207.3874559721 21398.2507802617 17303.3281801028 10382.1691287087 11429.0868593203 24873.7320700582 7815.44142429983 13255.0810756269 7859.83067844567 7209.6715105873 10855.0380403565 5600.33499828807 2790.64868634242 17450.203857265 2361.3215914197 1615.48207531134 150.564602293332 0 73.3146502310564 29093 217965 90 5031.83520864466 4169.96056685575 8394.77418575818 7385.12643908996 13818.6380837395 17431.1222833345 14824.8199364114 26492.3671836864 23162.5414452216 742798.347160918 21365.647940104 30611.3228282141 13765.0784174288 18318.2665889866 11711.1117023623 27772.0407745661 11687.3330811566 22995.7245064985 7164.91966167087 11894.7791936053 8269.44111691398 6516.15217416039 10154.4125517536 4655.48446166855 4127.74154085263 15649.5400029592 1892.39645735394 1399.61927983406 445.546132171891 0 94.1984449553347 29237 222514 91 4517.11504333207 4377.60489867655 9203.80035707187 6658.95457877364 12417.9639176394 13770.0023116839 14174.1618561082 22997.5976501052 22090.5643696343 701508.790726115 20622.1916635452 34356.4691280707 11797.742993446 22432.2802253262 12522.8307909594 9257.25570764082 11831.5660817164 22145.6481594088 7027.31887196923 12448.1974254295 9540.85539198734 7071.69445543976 8619.0230241479 3509.11478256301 3565.75113497727 13637.4432568244 1833.6948835161 1147.77605066923 290.251732287342 0 90.9009204811331 28980 231956 92 4690.64097032822 4726.91639317097 7780.09211580472 5138.43600254843 13676.0147128125 12884.8711795187 14309.6733285297 24608.1888078739 32647.6763717272 766155.875213023 19447.4489749218 33505.4538390953 12644.3652739024 24587.0851032558 17469.6676171417 20307.7393800883 12376.2176959555 22773.9098439269 7462.42175591796 10983.7167771252 10575.3815472079 9564.30121215702 11595.8259060013 4617.35817254917 2927.57852278848 14379.9126315346 2307.14220630327 1094.66411924716 235.122081000986 0 87.6963594486342 28799 212034 93 4496.08002347237 4867.95870103621 8795.35177136437 5267.1892967685 9976.1935267816 12850.2174295329 14320.3717554275 19818.9930919453 32243.2360243917 765243.964452346 19961.5459589152 34569.2874974714 11540.5969783608 21270.0026126074 17601.4731868326 18270.7321492275 12051.6767111559 23228.3568446303 7461.36387742858 12447.6150944056 9694.61547169216 7604.63904662962 11738.4245945145 4298.63464685631 2720.40765742193 14722.4809614678 1944.42366639996 1324.29809491797 452.140647484207 0 90.2724192690799 28735 208153 94 4700.61317314351 5396.75410524934 8514.06668584609 6687.21766154595 13545.9021161028 12443.7873048882 15058.410506948 22894.4905006268 24558.5771581297 781421.01909194 38494.7474578899 32720.6670299467 10693.6264886583 21218.6002860319 14012.8440764272 10222.865672427 13953.0676553007 22615.6342404946 7190.32904507025 13887.3895843199 10213.9718427648 7257.05887390894 10473.6379121402 4642.81092821248 3165.04409739115 13035.8190076827 1822.60787720362 1571.1808704239 237.138982441121 0 85.3232834631146 29109 213563 95 4222.97652481227 4335.51687137952 7702.62618896395 4759.02516565835 12264.7779595315 16474.9650878471 12615.7081279491 20336.2037912585 24092.7994524008 769707.24990323 19372.7984405787 30106.9670634597 13976.3248951503 14803.4591066861 13389.7478367231 17837.2587439653 12133.7112129495 22450.8628710602 8210.23226710258 14413.442542418 9929.29670986039 7937.74605540036 12246.1445706609 4765.21311807442 4025.15137673313 15178.8566063278 2057.77825639696 1649.26147923358 262.471408798559 0 111.187650413473 28674 205729 96 3981.5971432668 4949.14702565289 9343.23976468357 5340.43313533726 11267.9140946475 14611.6771299717 14700.4450747045 24384.3279603219 24506.130681232 733032.590740394 36640.6034326638 37483.2319130317 12320.8745879492 20725.0242662851 15908.1696241592 18534.159905632 13088.9280986024 24622.7284296563 7120.35196785146 14617.9953908147 9208.37122323158 7028.45277124256 10352.2133805488 4722.29293994577 3306.56881822042 15421.2922194097 2454.47218865411 1860.87572496069 393.503275698778 0 76.1701120511794 29157 220531 97 5460.06805368979 4890.30553233751 10187.2736478672 7116.67162486296 13837.8504061774 17341.4801404659 13752.2742233541 25762.1954584428 23473.5472810927 832440.104886984 39485.2339310848 36433.0617724106 13829.3772957454 21097.84707815 17366.5342777053 46191.5958571387 13415.2557135919 19999.496211264 7423.50243237936 12809.3883927422 8426.06169151469 7652.44174531514 12516.1801854711 4598.73988297557 3003.4110487534 18228.0071250559 2147.79064660272 1490.61330196643 782.742771359226 0 72.1476014394868 28745 220872 98 5272.67323668741 5810.81104959426 8582.79843320756 6682.89253229156 11348.6086283247 15512.2838539731 14813.6341194377 24779.8169151543 23679.1919483883 780092.524315736 19530.4726605408 34902.7974256374 13669.2063029444 18815.4605977678 16983.9958139712 9211.73653745912 12633.6971123532 24214.5999691836 8759.27439603545 12984.3668033447 9194.02934006295 7934.07440985947 12916.2434200029 3858.40711591963 2824.77676027209 13169.9595172839 1849.49014615234 1373.90881661794 356.964093391662 0 101.578870297572 28862 211282 99 4513.39094786726 5688.73675712574 8187.05118380141 6707.26179767369 14562.3017725223 14907.774173957 15520.1511957897 23901.3757419631 22721.7908037825 752134.459128546 20805.5725276803 32062.5056701939 12528.5694668127 18892.297866363 13618.4509462043 26592.3990150801 13943.1067273359 21094.6572889844 8662.84329726486 13594.832690851 9311.70116361942 6196.47803692811 10458.9370150552 3890.86696619318 3796.20885225195 13681.8543763625 1862.16654340202 1256.34168148711 508.10390909861 0 101.288111694219 29283 229348 100 5288.9029237506 4576.70994089161 9060.64395703379 7213.13521158653 13779.6243415596 10922.0323263347 15421.9658799226 21616.165862557 32715.858850332 888793.986367762 39696.7751853739 31334.549726654 15045.012232116 16737.0573626676 16885.635098133 17217.0624529156 14388.2718783404 18925.5490787587 6821.71905058308 14144.8163246178 8337.80301816691 7726.25201874515 13705.2818613921 4299.41519585532 2960.20756051708 13988.25603082 2113.44982396292 1363.24040356975 223.729770385792 0 94.8100609616023 28824 208389 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 4758.7714456453 4761.61393901435 8362.85682322858 5804.29772310928 12366.5408463392 14305.5475262267 13780.233263408 23543.9490876291 28356.4972213287 772910.309716773 27765.0875929036 33578.4468674109 13310.4210342356 20122.4155190669 15868.3254586408 20685.6164487767 12983.4180809012 23491.1830841312 7751.24105452436 13088.5761623781 9517.12132933122 7657.84242729147 11090.0084244218 5005.98841046995 3323.03607309781 15299.7950833371 2124.42728229467 1360.7504154321 392.750105669487 0 86.4915788242977 29015.7 216470.33 DEV 458.776541061264 469.514806581003 1021.4082249039 972.578822059305 1086.23199763955 1941.75363896773 1073.42029506686 2334.86181121214 6108.99615821245 46295.2856960193 9386.56376217939 2736.78954184069 1758.2941062406 2399.50002349604 2107.96612871696 10662.2170911674 1229.35145636797 2012.2556034068 689.455694654886 2161.13842305434 774.157248654841 775.557461210112 1290.04768280955 884.895034827193 726.830652997377 1234.86563385147 281.05729379188 198.104198391465 149.770433058149 0 12.7249861398412 281.654948474192 9194.75798491184 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-53_preproc.map (3496970 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 6427.27167031914 12584.0499271148 9079.67626886733 12111.7003970945 9635.68398647988 11071.5027817596 15495.9748339401 17463.2750574299 29852.1982298458 543838.181005462 28017.1025351073 17646.1138186121 23512.2866872147 15749.0112073044 33088.4489929754 279472.059908756 14015.3776851126 47474.04169372 31420.1185820826 26371.6864936143 30915.6271904 8984.92349120567 8417.05291562117 10037.0055004437 2697.22349865226 6079.71085990838 6123.23975504092 2294.4558902426 152.306689901604 0 8.67380699143166 30519 195111 2 4746.17354070375 12152.4481271176 8874.58997918312 12434.8500935725 11316.434195795 11922.6295485622 14148.852678449 20528.649574355 23075.855771908 587576.670972081 25931.5037814976 16567.4185275923 30030.9197919645 14964.0465820307 30031.9363482515 276685.716285119 14444.0481271129 45772.2343794975 31246.969000171 21723.6742242401 31560.9848022528 10208.6867750895 8377.13430712797 7836.14435963417 2948.80956249899 7577.97145641298 6256.07021899152 1753.05781780782 131.086552904837 0 9.5227048105326 30263 180901 3 5171.48717650765 12908.3521829282 8435.21333055992 13752.832515593 10407.7731677547 10835.7514967212 13345.6665897924 16839.0604443383 25452.6723033311 498414.406554889 23387.3441606734 15200.0193892081 30919.8797939411 13627.1402789036 24962.0053360191 277326.403472754 14609.7171819737 45192.7954697321 35880.0112935818 28155.4457843194 31979.9211500918 8731.56181093527 9102.67310690071 8962.50554612056 3376.49967216334 7050.74611686126 5759.02489682642 1978.8647366408 206.247102159034 0 7.96228548399858 30493 187218 4 4964.80546392996 11565.2028168413 8093.90071215205 11344.0510405161 10258.915679186 12218.9446990179 14852.6156806818 16970.4431990054 24910.672562196 499712.954947481 28562.6628035956 16857.8009181235 30969.0098918046 15173.3929343481 31613.9479653506 305047.021287359 13810.6096333767 42679.129281689 34337.754358104 21561.8837500714 30770.4546344578 8588.68958145958 10913.501214524 8660.52815824509 3159.93841928718 5618.56183636137 6700.77934385517 1616.20559976627 142.54402836244 0 7.20984489262894 29700 179973 5 4946.12168490185 12816.8594582035 7445.78738415234 12685.5779320962 11240.1436347559 11778.3567014085 14538.8620930232 20919.936956313 38491.0804559957 562201.436612078 31432.9597832033 16910.0375502495 27348.749094626 16805.3596712051 31133.823210081 268929.146020837 12168.1659046783 47727.7714899411 39788.4119927543 27441.5177778658 36009.5033642653 9336.61096114063 7600.98041419993 7701.45020592064 3336.9242032603 9190.37923042944 8038.49708398314 1422.81834901761 162.687056911875 0 9.34400399026249 30007 184575 6 4295.05358981672 11124.6386000376 6098.74679729771 11170.3137344813 10766.5684518063 10482.1980775626 13351.0873507564 15661.438660539 30061.426159301 569901.517345088 26606.3811727389 17533.7553045498 24010.264971462 14266.0300192918 29843.5082355726 313706.27845629 16791.2722007258 50824.2138321197 26141.9028073043 25404.1313801225 32515.5644957331 8625.21319176479 7571.82540061564 7900.40109801371 3084.81299815572 9303.58510686495 8682.65022432239 1543.29876124973 153.205885473947 0 8.03336634440583 29989 187448 7 4687.73671566507 11890.8808929295 8319.07243553316 11646.5636632821 10289.9086263321 11366.6973915839 14071.8833018077 14928.0846855428 25925.5753004936 568770.31917149 26406.5599202749 18245.7649918645 26741.8794264164 16510.8093047015 26643.3648081393 291435.547678249 14443.7087150851 48947.9570845494 32643.4578266505 22478.5316497337 33382.8196437694 7890.86969112515 6818.27129929535 8814.03951486189 3305.13373343362 9656.73981391575 7844.26044860814 1359.90531575852 121.920987318712 0 8.68611746079204 29975 184752 8 5485.84420326781 13207.6307151445 10101.2905751559 13935.7685174942 10258.4314704257 12738.1845605882 13728.5551547342 18194.2056013344 29901.0260559672 574110.158118535 27700.537813702 19456.5342130843 28367.2612965368 14159.5747154979 27064.7291619497 291623.170367841 13450.8518825759 49747.5204408607 34090.7035674807 24310.6785368755 32298.5725886625 9131.14751501128 8435.66902406611 8923.60417240777 3203.38519949043 8019.60289134706 6213.31993255176 2073.71071077354 158.742587451674 0 8.77845760351237 30066 178516 9 4322.97613221357 11528.5898084198 7821.68414138455 11428.1183632666 12034.2490332466 12639.8927289304 16068.4285041218 20043.6951270352 26020.9583360906 568292.325691151 29012.6218378324 18744.1995289457 31775.7934066011 16129.6829015468 25056.7348795925 298102.037689826 18675.3678349364 48922.4025583258 30773.8597520427 23162.2419458681 33608.4326369654 9387.04245394942 10513.3570965607 9104.37001208544 3070.77957815026 6551.87561487925 8363.18369990308 2688.05151306574 136.474783321908 0 8.48574855534777 30029 187685 10 4557.89683990275 11585.4041101475 8648.32952424122 11574.7604906446 9870.38631953266 11848.6322728267 13726.6809813118 16805.2710858969 23959.9776910348 492432.962117181 23490.9312862732 18900.9804513782 30131.8075083154 14277.2111399139 28286.340825691 299976.52558592 19171.2195309182 44919.8694097001 37106.3816648041 25250.3877982169 36466.036562746 9348.10371133606 8479.24454241806 7837.87403160701 3341.00767906452 8236.24989361685 9183.87782371764 1828.9202577826 138.369487345218 0 7.22579066086302 29161 187178 11 5397.57636062894 10602.5012601817 9054.64286619042 14756.2545835035 10543.965331142 13056.7874600037 14611.6172064075 20817.3365685973 24452.7523123531 554215.580341943 24924.537771461 16344.0880424268 27679.4911701303 16857.2206668507 27623.9332976254 301948.673318718 10282.3240511304 49269.1125428452 31818.1947294876 22228.0036033129 38725.8334147443 8482.66819718267 8243.32671257341 10010.4697554096 3375.79962084613 7589.92376640246 10172.3832488995 1305.68444723381 204.448820384287 0 8.1407116730795 30561 193176 12 4404.00952275325 11060.3973848606 9766.39912862976 11713.1028816129 11398.2413069122 11189.7084094873 13052.2461322902 15151.06379333 22224.4908045991 521078.595681921 27821.9623233009 16043.650912123 25197.3129911256 16002.4779726713 21184.598348127 279994.126561108 10693.4572473468 42606.7062189038 24222.8112401325 21316.0472628471 28534.7366130907 8381.86020652263 9775.80120751817 9027.15696707533 2484.83179357871 8450.27833305836 7028.07141470903 2578.78499666131 145.547298480937 0 8.2706049652413 29714 185561 13 4641.38661616662 11838.6325406085 10059.4396864145 11403.5324843291 9899.60005901716 12411.3490421361 14678.3939159143 15924.0809734201 23584.3719953941 475398.578823056 30000.2544773874 18602.3149160385 27856.9104743628 15095.5739931238 27874.9614773327 278138.307508165 15663.926516421 42047.8832748963 29204.1489307446 21362.7135702527 33748.6714153296 9197.76547356958 8455.0880960504 8611.44522591587 3242.91727057698 6044.91943770461 9869.75716622434 1963.91613053319 207.871962393589 0 7.56215227313729 30030 181713 14 5484.67763590888 13158.5345434025 8857.13249257036 11615.8235478992 9164.49199567584 11223.8657249175 13675.2377203178 21598.3931052215 23157.1516024366 534851.533188117 25790.429408164 17368.8075751517 21931.2011208487 14200.6132114649 25690.1272778332 318238.948439449 12416.8859295373 45139.6317424858 33539.3582017611 24702.3886248196 35413.5549342624 7780.1964323529 8891.73483847915 7392.43063643 3766.98668994798 5800.48750493864 6541.13322567049 1090.57338756675 268.336315705608 0 7.40956748741769 29756 189108 15 4796.13100281114 12532.7757486448 9262.40964823443 15276.2425243147 10645.6334689565 11685.9984316329 13961.1761916523 14501.2647572375 27103.9673415576 478772.105954333 27594.5952511175 16678.256442763 27685.926966359 15269.1963926526 26917.0416279398 302015.477717456 13704.0601411214 53879.417114906 29519.0331177568 21086.8773959376 30020.6016305474 9340.1292084989 7999.25003765751 8037.37070872457 2850.10520512737 5420.44993299207 7943.17832679599 1734.31267762418 125.947858440199 0 6.94603214099949 29739 177622 16 5083.68286707269 13792.3781049693 10476.089475807 12442.5511434165 10055.7852222088 11776.5698616426 14372.7451564013 18036.1891241833 24457.9934842723 536996.331003368 27861.1720885638 17603.1680842935 24656.8327503195 16520.6667500318 32204.1239660234 258127.115713703 13317.6473008752 38017.8819727343 35946.719432885 25262.267153326 35180.3473588983 9587.59908976813 7139.94105340022 8259.18859338274 2689.56717173169 5782.66393703394 4334.67943303726 1498.95944086239 132.240078641431 0 9.34574589926006 29852 174682 17 5772.40450090188 11908.8651845907 8527.16863325416 13111.4871020605 10423.6639248114 10427.5784557471 16377.9887229369 18819.533007803 24481.2994186506 566356.326924707 24961.7874600829 16822.3918924895 26423.9484528443 14320.3239202041 23927.5472842841 311455.893458032 14367.2482263119 46244.9439258912 36325.3998651272 21749.8140577956 39093.7345691208 8474.31919773553 9673.41938545788 7152.6828849346 3246.93657558714 7748.41012743348 11116.4833383448 2061.48824191801 128.367407516323 0 8.06064883254953 30102 193348 18 5143.98517951144 12446.0367997995 10009.3809152959 11185.6353227624 9563.8363497058 10202.0195012252 12663.5194286116 16087.1345013949 25161.9308982506 529215.676742026 28147.0957831545 19125.8567828147 28690.8344793237 14820.4238789976 24615.1779234651 287000.674653787 13942.729553616 44707.8295962396 35637.3025140688 24354.5251766826 32430.6127698 9381.34017336352 7923.60906296259 7890.32120131608 4582.49576670456 7643.08694979708 5984.17280878965 1473.68510048068 208.981783097441 0 8.18333966469303 30111 185412 19 4815.0656319987 13318.3930790769 8935.37865582898 11438.4682974721 10075.2082377166 12063.63329711 15224.0959815684 19857.6908092072 32620.7794305417 563748.681679384 27681.8997127929 18206.7855828129 23822.673299486 17165.7568103902 26439.9835464591 285111.431469161 14208.8027131324 44939.3964350511 34218.4959463811 26074.0237546851 28910.8125208138 9237.56244947927 9071.84027467566 8642.19940136998 2911.85602914517 5656.45936707413 6815.30533683704 2083.19727374541 133.392902744449 0 8.82963539470806 30434 186687 20 4857.58955400951 14997.0034776193 7790.83672277942 13601.3997801256 9337.41163539256 12014.2326088469 13083.8501651264 16680.551240965 26507.8456953615 527137.099243815 27619.8815659906 19018.9444621927 25758.5831505155 14747.618660849 25788.6360636666 344866.658477328 13516.7940707104 42734.023733626 29363.8259468557 24388.017903241 34894.1253320428 8300.87030891231 7219.65524480367 8639.73591149452 3114.88572507964 8860.88837366673 6977.72508276552 1448.67569025513 133.374507467221 0 6.69695492658328 30262 189757 21 4812.07922036625 13244.9584983229 9592.89504223421 12374.5894504452 11197.0066297994 11352.17712796 14258.4735849989 16848.0364453814 24328.0947011755 552961.896610187 23464.3867913112 17011.3845232271 23067.4006088048 14897.8962580017 31911.7348479908 277117.064031595 13278.0171000671 49315.5987392084 32267.5069693184 18795.9582700796 34309.3612940607 9545.25135780423 9642.78440662467 8417.44454264412 3557.39979982897 9255.13078048951 6158.28640572456 1898.75420994838 111.618481976061 0 8.89612571593086 30265 185760 22 4875.85179580884 11771.2069207059 9499.41075306593 11646.0028768258 11407.141116089 11626.5474732174 14884.6176519957 16329.2463274696 30565.2484998606 518613.818409837 28589.555569219 17895.8309134941 35376.7594562983 17272.854909066 26571.1829932299 277038.76314002 12646.6837828681 41651.2162032896 41393.5395680247 29025.9482256051 33743.4875493876 8781.01239466192 7267.19861274239 8218.12793742039 3596.80090733885 7807.72523875992 7058.97496736339 1971.16946902456 177.240485371106 0 8.31839399392721 30173 186589 23 4593.20281929579 12877.6041189965 7329.17300722978 11921.3037737357 11379.7910212805 13347.5551968847 14898.3557987266 19939.5513005719 21695.3653755971 578141.574686522 25070.5630123116 18209.1412342122 27992.8073422114 14261.9908810571 24590.9871238317 323042.405615102 15393.4974780507 54719.8421671467 29869.8715730763 23884.3206842276 35331.1566786475 9513.06619466072 8265.95382164172 9213.1952365877 3382.80391460198 10932.1806514672 5078.7543908536 1787.2689049783 124.540478359619 0 7.91035948632493 29867 183983 24 4941.07515969254 11511.4310895538 8077.30115029023 11144.812667316 9975.37869164692 11907.2878916982 14598.2104618539 17201.0328251042 22535.8630274949 538634.280240929 29679.6780872726 17455.9428254425 27106.4442481348 18240.1765159404 24239.5772060194 297089.464222406 14222.7403943341 42621.126367161 32930.917677258 23444.3826638714 33629.5042749231 8133.715101975 9468.2518816869 7941.7298563366 3370.50775964602 6223.29720979192 6332.13269753335 1409.75609683229 193.20990679797 0 8.04463032932752 29976 182759 25 4667.89197442673 11817.7836567624 8664.01062538344 11773.3573198899 9079.47238505111 12531.8789270796 13663.2468721923 19185.5599645917 36590.9213088846 533507.284548885 27954.6460611834 15993.1020165506 24298.1557400178 14652.0567496685 27669.9169800901 304150.419703882 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12697.127802829 7669.21886557919 13418.0237806399 11808.6028620532 11385.2605954245 15623.9103043884 12985.9705303755 25989.282698286 547251.515731484 27636.099933545 17211.915653798 24420.6653690451 14282.2435789337 25812.5761001892 313178.893337139 14664.2618673813 46742.3928929132 34247.2104767826 21465.5370424574 32317.1925636892 8491.69739078676 9187.05591100743 8255.55394943551 2800.30832750057 7233.94305429332 6228.96804893995 1441.95790005183 118.623221243913 0 7.72403704338469 29650 173114 91 5981.14630949336 16573.3636676408 9393.97284789094 15210.7105213389 9536.87519411182 11615.1187966429 13373.0753515348 14752.1644064178 25493.722772128 540270.568190312 27751.3559132609 18143.8165284619 26939.0508066267 16114.036832937 25933.7973095225 292772.159530882 13404.0151637516 41690.2974910063 32772.0535301953 22401.2390002158 36071.9920408846 9692.17998107401 7760.22609001295 7712.28573318733 3035.98532618978 7513.6024795727 7412.04161460385 1740.90389584119 111.972592366839 0 8.21117714800354 30265 184100 92 5041.61567349763 11336.4783654249 10352.2200372937 11474.3198933515 11207.4310158412 11245.5593663501 12398.4530623942 16212.0330765507 23578.001303665 603670.975092512 27572.7610403833 15970.3954088877 29222.1899099102 13742.0391941645 30446.4641517386 283058.671515991 13292.3234164237 44804.518114228 30453.6784274592 19158.6674260513 30621.2429867654 7811.22915563718 8833.12242246822 6295.05237526585 3405.24812640946 11854.0418449339 5832.46783759753 2041.24536152724 198.881824073055 0 9.54937136471606 30162 181102 93 5004.04637774272 12727.8573670472 9033.46329148506 13537.4076180636 10786.7443640828 11725.055955986 14596.7825281723 15929.4226704114 32042.4549010211 502910.504030921 22313.520905852 16231.9029113706 26328.6201466289 12448.6689568094 27936.824364257 302363.958085491 15074.0086731868 42843.8749960841 33088.026586466 27783.6417285959 36275.014241938 8161.96390883143 7560.33808892352 7534.63001649563 2816.32631073736 7159.86950364016 7758.27167497244 2181.22855066997 117.86987246687 0 7.33332885457705 29926 184369 94 4808.9539226843 11347.6420183952 8344.98609113946 13440.853083438 11424.4620435144 11381.9775655013 14826.0646169142 17508.2615648615 26568.9998796077 519406.981609076 28139.7314922334 17930.819501602 28444.5324494063 16133.8855127827 27471.3873644963 302474.002611559 15751.2080016449 50644.5249037468 27972.9164106892 24202.5867000425 30331.8905560618 8422.28669228309 7218.98359037406 6820.06344274728 3244.05654650025 9113.57763327428 7759.57429701635 2090.26430668557 132.377482361554 0 7.58196378100039 29975 183841 95 5186.06896934586 10582.197518815 9716.78939469877 12224.0280225842 10601.6335348997 12502.6744277859 13766.4551391268 14650.5060640357 24364.4958781203 513566.425210223 24610.1473585239 16073.572273713 28795.2887048105 15784.5219114286 26987.4261278595 289076.939034887 12090.8838176161 43146.8622384098 28955.7009065752 22208.8959138755 30788.8397627859 9990.09882938883 7492.87503433804 7444.41682287372 3110.64138171235 5695.17017389209 7945.21007841971 2804.75446283561 232.607124461204 0 7.87148005837201 30321 187911 96 4451.03175628194 13520.5537929041 9968.66767294435 12377.9517338462 9490.86622833342 13159.595924444 16070.2051491378 25828.3572641871 23794.6937178037 572799.237132963 28089.8781395664 16953.3025382289 29901.8767247803 15048.2015066824 28595.7922605906 267765.817737409 15311.6061891759 44432.7754379515 29372.0027174515 21127.280366919 30690.2567825686 7700.37820812931 9413.17513398658 9198.96343002406 3088.70650730629 9166.31508781474 6677.18125665149 1204.50079766379 214.924465186654 0 9.61827599448897 29783 183895 97 5131.87677576206 11123.6514313943 7890.42486925937 11543.8904150556 10066.5496130358 10748.4964964729 13474.976231496 14474.7888284888 26339.7198628264 517899.575820455 24445.4424414588 15321.0427827695 22189.6669247744 12522.1635222124 24941.5761022812 306968.706187815 15589.9462245922 43492.8839181771 34902.1053840105 24692.035475736 32759.0666962558 8920.49187688099 7273.25792158109 7164.34711888097 3121.8854518858 6182.13742785144 5539.2216127337 600.619536820579 178.021381242114 0 7.43485739579579 29701 178632 98 4999.16809947078 11862.4587986781 8575.38254713097 10385.5498475632 10356.0715275977 13692.2765208264 14591.1229483412 16871.6155215354 23755.2118089026 536906.483979103 29237.9693852204 18032.2576327202 29431.8856449705 15430.76467434 24533.1154303765 265092.342096716 13097.0290584817 44685.0560661321 31793.1389363067 22774.964590611 30747.8163998829 9337.04152720196 7567.74475817992 8011.01888513463 3255.54719660022 7604.50460337148 8071.76526501707 1367.28132716455 112.338245544474 0 9.05734584225207 29931 192359 99 5150.27720284236 12251.3467924982 8217.22553406694 10790.5359810292 13889.3686732983 11566.3350157625 12801.8406724009 13918.1085835271 24656.9932398526 587530.770471356 25295.2575443275 14209.3393110239 27542.9763839499 14675.6025012682 28943.3380062265 278251.713118772 12909.5179655085 50860.9274208 26007.6942656675 20526.2838314733 31645.9147300592 10352.214389164 7366.13895322207 8329.40022084118 3062.93234353471 6289.01320351023 8133.25514532389 2113.4558085468 154.010140975707 0 9.4332294044334 29667 179316 100 4617.28729292195 13058.6657068294 10184.9340366503 10702.2399973834 11422.3982809075 13276.8581596417 13505.0110168802 16362.1780497952 36844.0674953315 496056.469718183 23815.5934766796 16720.7010319079 25027.0002754507 15101.4613626862 28585.9336247078 312096.735491877 12246.8001131954 39610.1859044513 27046.4685789587 20563.7536749495 30502.0806221557 8685.45390482399 9444.49387564644 7674.16372294617 3631.60101267723 7657.5848442519 6067.00567468221 1048.03259784324 232.605119798845 0 6.98583382532976 30232 198910 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5120.88343632904 12507.8924209876 8974.05133158653 12321.4617007787 10375.8239007887 11880.2313507967 14398.1645896683 17599.3570675834 26685.9122369721 545780.511191897 26461.4093623991 17032.0460308288 27088.6514379779 15263.6166136638 28118.7364347235 297828.469256845 14150.1257037479 46303.0635114832 31884.6387719469 23027.9700313209 32989.4368390573 8814.01854300271 8235.55774072515 8155.78202744655 3165.34208138134 7765.99515365956 7157.34874071659 1694.80663364495 154.074387761053 0 8.15599629920941 29944.37 184529.62 DEV 538.361401882498 1335.3217536811 1067.81384927075 1137.97763407943 931.446797188986 907.454540421925 1023.65197419464 2531.18757051513 3910.58128931173 31768.3782341935 2019.68459141751 1130.03350986574 3036.30888135605 1438.02802725598 3147.79564190144 17082.6918919301 1644.63679089647 4089.89087649524 3812.77357265796 2171.60245318195 2511.28871212325 733.708195418166 932.132589765103 967.668959669203 336.881056344937 1536.95149073781 1427.60005210632 442.022824864652 47.8160469591908 0 0.680285034665991 291.847893773452 7070.02910995421 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-57_preproc.map (1585882 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 1990.86312898469 2664.54202747568 2353.73690089292 3417.12742822277 4912.06454959577 5393.25822197246 8527.92178492165 10922.2035996343 29446.7947643042 426475.366349594 53554.0724594504 7956.42573246382 42455.6335313613 16060.9558641959 22967.4673551776 166285.699989279 6204.03365452576 21747.8302950481 9177.24980728385 4534.47737105098 6272.98164829122 2392.75153514843 2990.898981794 2605.25943718081 1493.43032893476 2959.6735609359 6483.11690975351 742.440745041774 373.502930934153 0 11.0117067415951 15179 118521 2 2021.08857367206 3615.09876995457 2033.5255738048 2913.76738381259 2946.77718273984 5119.12382428496 7419.76268643202 8113.02249108234 32415.4821209748 413220.807734367 55115.0258043717 9425.66362060061 40371.0522274451 12894.9108304723 23610.7526134686 157769.279775511 5804.11834199496 21616.3647894794 13118.4766933249 12093.1902783095 6341.1471682766 2831.16761477143 2295.79090486932 2957.70165363679 1603.60372496593 3887.27696296901 8556.37779278017 817.925960698385 89.9475903194305 0 11.1921509307758 14928 126063 3 1617.0850866074 2578.09824889585 2048.41210323252 2875.74060066294 5835.89411319808 5536.93512923986 12293.9743974137 13403.6147766503 24094.8648942844 381893.463339752 64601.382898533 8273.23534329978 33381.6119874596 16160.1594926339 24749.2622708597 191233.325216926 6492.8370708834 20047.6277336731 12300.6599550199 9467.23516483058 6045.89491564857 2134.6884961368 2105.64950441219 2572.02464304244 1793.65887313708 32760.5983836759 8353.34925447375 708.66782205996 192.518958679165 0 8.52319451673726 15048 118066 4 2135.47885736337 3309.86023014591 2031.03789461985 2437.50002273263 3669.70229449026 5452.3657666425 8557.50878319494 9775.0673962228 26737.2607788738 398473.9155004 64483.3291853294 9857.57321389088 42390.5521880978 12823.3123443963 24684.0203991616 197265.273551072 6071.86440359627 21725.4606386417 14545.1223520918 9373.45707509478 7279.67618365863 2384.21150748611 2826.75437693924 3007.32499686704 1663.91230348366 13925.6507430218 10110.9913335277 1105.1924823544 194.401013091782 0 8.588546949953 15227 131428 5 1920.62149015012 3129.77968800925 2308.65286547559 4490.27876515612 5612.05777090126 6056.00120427319 5677.53346249002 12417.9270135818 32490.3933792215 438309.69262494 41822.4984927037 8778.79444214341 58209.9712086816 14799.9473824781 36606.2929177039 225389.308267702 5594.2957759158 16940.59460491 11549.2931144647 8008.94857850227 5812.33547507434 2578.17840961279 2707.07766531684 2908.44368868774 1459.16384796063 12835.919234437 10155.7247666555 577.389041872826 148.078559056562 0 8.35434055522175 14856 116764 6 1668.94892989464 2626.54808426401 2231.45505574329 2745.89662537728 4077.97012241829 6641.84660047657 7317.83382765719 7330.26959209419 17368.7612079376 407207.511264363 55822.7024556412 8686.00295908076 40860.1523798011 12947.7957243098 24876.8614413876 198691.703162669 7500.91652905878 20048.6115903226 8989.99529753165 10874.2866500488 5685.93737562094 2876.34212654526 2297.37084575026 2768.25138868085 1715.26883996568 22001.366797004 10247.2013236175 966.179502955122 304.13952902806 0 8.81827716828851 14973 118888 7 1976.66594257706 2451.92806499714 2113.87578581415 2586.53099983276 5943.3399948616 5092.79609477958 6515.74425965945 13399.5427624352 20697.2745214639 421762.275899404 48363.5388237915 10139.7827698734 20424.0605844621 15274.061856106 21429.5313871208 157274.567424929 6588.00662699539 22238.8019594014 9855.15968845985 13921.7742649016 6457.397472151 2811.87102323226 2621.44015028791 2092.32968468978 1369.58935847702 32547.7822244272 7983.90166472522 976.324848231931 246.53416379212 0 11.7985184163239 14861 116173 8 1897.09133580699 2700.15085150296 1944.63147544549 2968.69580405019 5900.2583429553 5345.72243588136 7683.97508261005 12874.2684828298 16826.15547666 369707.557625838 44443.8484928463 8702.17501346297 12049.2688112214 16044.2447320667 25763.2972357399 190657.400679158 6733.49388132554 23263.773499543 9022.19319950138 7914.21889830798 6325.31786213595 2782.12401645875 2590.65193708854 3079.66421188721 1558.55429659616 22450.4635699845 11045.9501092441 922.479540878969 286.691579418596 0 8.45835128115486 14597 104676 9 2068.99619554026 2894.96322447597 1901.43322569224 3277.13381643875 3664.30732934169 5125.20774271673 6537.10716392373 6968.98883473351 31252.3975363819 423230.314395898 61549.2872651842 9828.7658463232 76915.589687639 13184.4594220115 17722.8663097106 150855.796792759 4994.13013280688 25268.570383172 11510.2892379033 6369.94212049183 6374.99658383144 2246.63495764785 2223.5130842851 3018.27674102877 1638.6569809905 42958.7458246445 8285.85836632827 882.108266234416 282.749039297463 0 11.0842036240981 15168 129226 10 2117.44823607223 2776.61387626749 2182.33687859751 2641.85250093591 3690.03352373261 5737.56105405474 8332.56172063398 8369.4650708364 26324.6155618364 437645.874430854 46776.7362294406 7789.35710766113 22170.2882804232 13966.6888706569 25520.9527815168 167564.161129257 5176.44000874125 21645.2696530987 12812.5328322653 18931.5350682887 7742.18236140457 2632.95434208436 2621.46828056374 2316.61603359666 1507.81119412012 13352.7858669909 8410.00773195991 725.395718690374 256.262890186704 0 11.4844361251596 15369 118013 11 2216.2804603994 3085.78779193126 2063.95690924236 3036.72535933032 4795.02228456272 5642.28723380366 8551.48960163129 12662.1067492658 17851.174465583 381127.510512858 40061.0517026996 9046.51615896824 42070.1564536141 19200.2808040803 21927.5270499796 156709.251814815 6071.08848287929 18903.745020448 14208.0881414008 8284.71060679217 7824.32966370247 3165.67261922749 3191.74512722057 3056.85516305864 1716.5925909115 3836.56144164005 9247.12327344437 649.598698379866 122.695291433712 0 10.6129945436033 15124 117297 12 1754.62640917744 2889.30679993888 1955.49448532942 2851.82729227624 4548.19443923233 5798.17230109616 6692.99477722981 8967.53700514309 21371.0503938819 365789.724133784 54785.2528779827 10285.1658012116 28293.4960814929 13725.0498197143 30086.3165522572 221470.142787699 7200.59131399825 14354.2335709358 12157.8071892969 8754.6306443099 5560.1846511928 3156.96694885513 2561.46647403236 2863.99695352424 1235.46554072625 12994.9897048101 7757.16890177681 584.177369278256 96.4876516675932 0 7.08788331898216 15091 113249 13 1887.62786202847 2677.97463726766 2289.86678811012 3287.96769373382 3323.63036493819 5046.78625803328 6585.33340044665 7951.84498497423 19529.6764492313 364148.975525355 51163.7444048424 7542.4257688241 35690.5837961023 15728.9569963794 23663.9591463674 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2505.27616193538 3210.72525342115 5037.55109413775 8178.97471204267 6852.16068916507 19995.5917365798 360756.004902022 39669.5347466271 7869.73453835202 48636.6160795516 15122.2185098702 27491.9212141792 190468.121580497 7136.60532791676 25845.4387145165 12350.0526078794 10074.4101882528 6462.72141001493 2074.86581505157 2387.47904132683 2448.90342760225 1442.42808656934 23466.4281110361 9796.97139014205 911.723544619614 461.774027436063 0 8.13631894533328 15176 110434 79 1760.24583375467 2527.10462580948 1893.89218335387 2397.11334002693 5091.72243435442 5740.20756868234 6930.39612302569 11111.4643578077 23404.0223430402 354783.592947497 58519.809804937 8808.61259072846 21208.2931305441 14153.0841593706 19756.9483205055 168086.927814495 6112.68921440592 24360.9869354949 10108.1058515423 14211.0506883209 6185.46723529362 2585.30894624254 2604.43126047519 3207.26331592433 1407.21140771956 22569.4163318607 7776.07700725894 815.592034370836 218.947302774731 0 9.0399731377822 15032 110264 80 2113.20535689183 2838.41405038887 2151.13623209271 2719.46444540234 5356.4085643248 6412.84418650244 8135.06102020763 10513.3242473445 26517.2321733631 406661.657082924 74658.3354836696 8337.03240991401 23320.7208036401 13967.3366005745 25394.947901347 185482.039469634 6033.71062285335 21252.8676488547 12106.2901505007 19248.4592973947 6454.07407122501 2429.8450233529 2681.04935108985 3080.56330140618 1726.33292396553 14313.6437516858 8612.44019331536 763.33306712368 329.591151695729 0 9.2602404233854 15124 120846 81 2271.48914322956 2503.99428892844 2137.20015157472 4080.1360373857 4950.64009614495 5817.37741025585 10427.5467674315 9243.11945134538 21742.3596433629 383063.502150967 61481.323949348 9301.15336501787 32657.8263535978 15839.9763516755 25673.000441412 158393.960948793 5103.59227344511 11586.8031691753 11553.3382775266 9405.94003128229 5585.59437200256 3143.86033424962 2547.06472858402 3648.40626433404 1299.42124926424 13604.7538955359 6492.94567614018 972.68477004346 183.132073530794 0 10.2929391184717 15517 118198 82 1830.73936655713 2832.73646209988 2221.24796472958 3127.57421454846 3981.6109567526 6686.68625458564 9939.35380428702 8882.66006907419 20301.2670528344 363185.183667433 41938.9747370657 9204.33685199416 41408.3930285399 13705.719179649 22429.3115760231 165261.536289331 5128.2004624138 15314.4381685465 8884.77409741045 8322.37244144612 4961.1060836263 3048.22782586579 2439.46515086206 3288.24013563324 1182.62941880523 32740.7578633668 10302.20620955 952.937175167931 381.285939318132 0 9.52415541549867 15036 116408 83 2031.73972662887 2790.01814116499 2032.00041702984 2826.90987352803 3182.10688021148 5045.83638823734 5120.44809519355 7505.52895461908 24193.5221686537 388233.633074249 50513.7606915322 7627.85138177408 35070.9591544913 19677.1537971112 24192.3820378573 184824.341503732 7501.76585591101 18026.6661037786 11531.6434482402 4666.51699959607 5926.91771559306 2442.08058645248 2984.32313414464 3523.20896307972 1378.81556750231 13558.1970399266 7806.9832750153 632.765285832692 233.950182506952 0 9.06284133431475 15617 134033 84 1827.13788103591 2229.14623391099 1841.0893171414 3260.46058380801 2910.40476654796 4808.60307961596 7612.88031297083 7359.28759645244 25847.1610987649 342344.549779214 37216.5331187028 9166.10850481755 31373.6680350043 14412.9777478492 26020.4687427133 182859.527014085 5427.23323257395 19970.8528145807 10209.9868381742 10757.4414122973 6887.11248094949 2242.30673349803 2777.67274022054 2797.05760474185 1634.19387249408 3449.4177254612 9347.56761499326 543.017883390569 333.835584413001 0 8.13048289784951 15134 110469 85 2186.62467920797 3389.45598428922 2031.82193732548 3323.12592520993 3094.81696696227 6679.34777803808 9645.10849647657 6358.25586033125 20750.1829567425 413662.9818959 52483.1743042702 9108.6905326737 44919.9495652333 14645.5459495258 25552.8131324877 197370.64520652 5607.09176688531 19522.6530738537 9845.76513204027 5045.82101823421 5653.20859320858 2035.78893175036 3138.80601178173 2824.56435798326 1325.83089599614 13591.4803326046 8438.28989718432 636.957157096219 209.040907302573 0 9.01952090457547 15405 112275 86 1945.49051911185 3039.09710053881 2002.72110638193 3300.44510505138 5939.13910439527 5822.24455065498 9557.78004338605 11954.7955424997 24911.0699022049 427749.485446231 41019.556253175 9493.28530991542 35838.3111950851 13857.1572138856 26471.6168403293 175755.28053666 7324.53905092824 21287.1370374014 8305.83098065547 8212.21407489136 6208.14456252016 2295.7307657143 2630.81997769546 2945.06296078572 1466.62984769431 13860.3387134418 10282.4124164554 832.390803248232 296.419275446775 0 10.7019911766151 15322 127150 87 1999.75808083272 2958.49147436335 1924.69499758169 3200.97366840793 3056.08125458278 5047.45495017182 8427.4103773258 5891.03064151172 23453.9693094907 384972.026666844 68564.586433763 8388.68674293193 32745.4283617055 12135.686367339 24251.816619288 164186.776433083 6285.38223692397 18225.3829656659 10062.6474246642 11093.3310091916 5986.59676100972 2594.63262221382 2414.27811313027 3942.24758859938 1410.79394110045 22976.4449466651 8196.37780351799 780.202303467336 269.762286176125 0 9.83590752898663 14918 105638 88 1812.83078587 3657.4936129613 1936.84650113305 3401.2102612033 4340.17166092109 5458.77498802683 8205.85517329301 9089.58190569731 21533.361163029 409312.644751691 60711.743573603 8294.30602758859 31949.9379402986 13414.3554109568 27295.6930849246 187562.794638077 5593.29493061603 19530.7395689419 11594.8710984264 8371.11548842256 5600.13067590175 2613.4118047868 2750.87848424071 2769.51856191899 1736.72474291405 32629.1494042589 10783.7209271041 914.685725488114 87.7524105568152 0 9.37511822710713 15409 133568 89 2396.20063771843 3298.29285228401 2053.13841950979 3179.10334020122 6884.60819673594 5923.10932534485 8665.63371344957 13697.7841975451 21727.9018860901 406035.735019 55358.549067236 9524.68676277251 20755.6787754185 18073.9557940667 27430.3312611217 191882.658849053 7640.03966178126 17962.9341956088 10463.0923495578 13134.0896247399 4931.35575927316 2146.7395498497 2939.78400362507 2858.45056459257 1527.61938031776 13380.52346552 7840.94962863154 1188.35920938771 363.026690535959 0 9.21454038882811 14965 108021 90 1885.07239913285 2825.75535525827 2056.76378584605 3326.38054701899 4274.05299255843 5978.63259572623 7406.84899913987 8961.64481543487 22917.8493629789 385054.280787402 45318.1494142476 8797.11649005243 50420.1309788858 16531.0312808165 23485.4271861308 173804.212332622 5068.24847043568 18619.1591888859 8808.66059716239 10394.9167190604 6617.74978815148 3355.1195489397 2673.76046359156 2233.36474264196 1393.56512350838 3837.0135554711 10009.0918456617 537.552381157322 257.018220347439 0 9.50751628573606 15356 131472 91 1741.16518186831 3032.28822734125 2093.25088125203 2962.75897213353 3112.22765783346 5862.17374753535 7364.58534132463 7788.42604134286 30647.3241414439 425489.511672286 65399.690549278 9725.20328413826 50594.7735209881 14432.486683686 23873.5137839467 176118.980537962 7816.87773898936 24303.5571272968 12027.8768098134 16415.9085328955 7342.16560912525 2216.44856171957 3010.23257193528 3277.22672073372 1480.69353856291 3649.81174483791 9366.26950178612 1008.31820459487 217.992521919135 0 10.1730371843885 15083 115890 92 1752.53350108402 2271.2329888168 1972.24471748116 2702.50344589363 3343.97577973816 6365.41549309123 9463.92527535294 6869.81836847713 32423.754947916 406470.735318619 67921.7313431754 7715.05016578466 31455.1116695948 17461.2142068652 23607.4749052465 158944.469943275 5649.47348106836 21351.6866166784 9867.03622452857 5921.5871204598 5405.87755195558 2670.12884006054 2651.17314744473 3063.52049645489 1645.36173071781 3803.99930726251 8071.12647660623 814.261909971269 236.693229338633 0 10.705363984944 15250 127785 93 1921.83331520347 3151.64794801551 2201.94893439507 2521.14319218768 4142.92167936086 5317.9311560436 8526.40469313291 8475.52456772303 24151.5153049338 398234.592331029 40064.9972904905 9309.89341591145 22802.7094360193 11783.8840976156 26772.7743367212 195112.987850628 6780.94449190877 20591.4769528519 13015.0553044555 18204.2411439699 7200.84968979365 2726.15972697166 2575.79664406118 2628.89193536732 1177.40795893887 12646.2404599926 7483.31886430813 866.311585071916 187.882396362256 0 8.96416132186655 15042 128756 94 1783.87648686112 3063.09226062979 2587.34754885806 3376.33593412795 5737.07366931112 5584.25155532068 6652.59598282283 11207.2829285623 25800.164190312 414430.418568255 38640.9175282434 8660.86810223443 33654.9401319945 14637.8759921285 29340.3507130737 206416.557617337 6985.10292519747 24006.9126241764 11115.694332077 11082.4448954149 6091.59483541083 1887.1964475827 2286.50870537303 3505.5261338283 1394.15108347879 13434.51716365 10241.0993996868 622.359735447967 183.299914455592 0 8.79363216401104 15228 119827 95 2145.63837735273 2891.45452247527 1977.54976287936 2752.98760565012 6160.01814660033 5356.16054105617 7440.92645861141 11965.7526002685 23481.0912440241 366248.416126337 62020.7782017418 7116.6716617442 12345.6828132625 15814.7632295011 23099.5838058687 192293.898871934 6528.99077914356 15202.1564450012 13255.3574617032 10223.0042695355 6323.37678163005 2013.83189780067 2266.9053245385 3640.19079122231 2013.53366901942 12594.0257182259 10112.0875859169 1005.01501068462 112.803826984448 0 8.1532851499908 15069 98871 96 1833.4955151493 3470.41683857906 1953.01904242418 3482.80451268129 4221.97373206126 5537.76976337059 7850.57338210932 9358.28112546823 23639.5258255199 402432.383694366 58521.701008977 7940.48476976146 46181.0559405498 15151.2498813112 30164.7532998985 221607.72713428 7153.04468768664 24238.3495099744 12608.013555718 13940.3283985516 7551.77212526268 2755.56335789301 2512.46282430201 2722.30678317154 1625.36356728543 4012.80461491926 8936.69340646169 1053.01509851804 218.896300502828 0 7.76845375114861 15048 107569 97 2119.61425350978 3529.26154061015 1843.38100817941 2642.09165286371 3369.45547833767 5516.99090714315 8886.47158953601 9045.56669279377 18256.0573854099 355111.279961673 63267.1267203845 9187.23271158821 38190.8349147013 15872.5940614721 23595.2696924741 164276.800909624 3968.71905368155 23675.9856012995 11663.4431239112 13329.7770664195 7865.41893056995 1941.32579528846 2972.28631836203 2594.40984391852 1686.1623523747 13251.9966102333 8917.08283259926 1046.95492249136 189.712311496552 0 9.10256558038722 15117 131698 98 1742.36842141686 2306.24118043266 1852.66370249841 3021.65932855035 3091.09758224742 6518.61553745315 9292.73575364351 8699.14068508394 24114.2486143212 358461.884906496 52065.3581762589 9377.85313413267 40224.4311610879 15233.52736533 27652.1517474633 192387.551231265 5359.92149079207 25760.6695616147 11785.8837888643 16091.1974401627 8531.50959053226 2335.39197176931 2167.19723750504 3990.44381050681 1335.780201398 12980.022805105 9117.13114436517 808.625198780275 227.842594795113 0 7.9810881195601 15298 124444 99 2027.23726875402 2828.08632293351 2151.81278729849 2301.62160987145 4010.87410392073 5670.47836380688 9690.87182260637 10070.5646461405 23303.5014414894 378194.286570095 40028.7358872414 9017.05585467607 23947.1801224723 12874.8746366453 23834.4996280758 179411.168336216 9215.33406771591 21215.9784625769 13758.8172296503 15347.6153358126 6351.57344459016 2759.41355305649 2413.12212854957 3292.80477252657 1701.90747803392 42116.035517762 11592.8025818379 940.975833924897 166.327518122884 0 9.27706541179814 15136 109862 100 1930.07533422715 2706.55906324194 2319.73016057791 2553.68926372223 3101.63364725332 5292.87370860271 10050.7171380204 6986.29181383306 23251.8092516734 404241.187460877 47241.879240536 9583.98989016849 12214.8109057382 12718.3883708993 30952.7075119036 206517.172686359 5727.56919969937 15668.3913842597 11543.5321718603 12989.3811561055 4579.73504444153 2823.26140532217 2609.02532263695 3133.69244185478 1445.15051052689 22882.7614672319 10215.5588975127 898.708724288153 313.005951256852 0 8.52514052080536 15160 109175 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 1975.2172075535 2937.12043794332 2080.14566391036 3018.64702073121 4306.03394041569 5585.76160484167 8077.48186478101 9256.26186369634 23698.968369241 388359.499252647 52587.9254903539 8803.81587228577 31811.049056841 14863.0758619289 24670.791005672 180430.040173616 6211.66364226151 21057.8625871808 11062.2019090071 11759.979729983 6353.90220893813 2625.06502156875 2676.78155002764 2985.00952203367 1509.65723054463 15427.5010233001 9088.7491211475 831.26545472767 227.982666224736 0 9.33086464709441 15156.09 118546.74 DEV 218.887905624811 362.617826626996 205.907057909583 447.611417577898 967.442582778061 489.520927307679 1342.06162899524 1908.09278106951 3902.19538581137 26680.2383922072 11315.0448944739 812.704828514632 12701.5456599069 1851.10737166845 3281.53765592702 18046.3506840771 938.429187754873 3643.52763481831 1675.28445502466 4523.51724113138 804.125727215624 442.238067281002 368.988960761641 521.278158734256 188.458004575622 11258.5025978481 1151.24154614785 178.209675641589 84.6567079133078 0 1.02366379821736 191.587217475488 8574.67322015247 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on POR-73_preproc.map (4059217 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 6596.95704465174 5983.96134292307 7086.11499848704 4720.05861640568 29372.8878637888 29158.7826749005 23594.2823991294 21156.7466071823 68274.0560144557 1330158.3479775 57292.9002245834 15270.0778073828 15192.7911023491 121987.596077665 37167.7880673985 15458.9912095608 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12168.2821594646 8654.78524241045 8296.85412594433 5446.65640096314 9754.24754468543 3962.56826435227 5384.56244174191 9456.91978705692 1770.0466822924 2852.35722856864 1734.32617226507 4220.86974373556 246.288059684135 0 46.879412962922 17212 151665 97 5976.45228229218 6019.11950709124 5989.04902068048 4438.28049479553 30551.9054068257 29349.6765144898 27575.5797414776 23798.5739595846 57058.1985190379 1299580.96526612 57972.5439941007 16070.7771931917 20574.1597139703 114729.210442168 25996.6351410607 14116.2898870042 14277.0024714976 8215.02207103174 8612.64443323156 4912.99209924381 10208.4203015081 3938.42300617963 5904.87151207569 8685.84278480341 2502.87482912678 2096.51583331629 1733.58468500571 4297.05733337901 293.689864976294 0 48.6913724519553 17650 151946 98 6721.29802472462 4995.75204315248 5437.98663278516 3613.61106836836 34533.8821735276 29235.6061228046 25650.7947866261 23923.417400392 66652.297315009 1236196.81815441 57364.1137256293 15725.6541326623 17553.8573588547 108965.65929757 42640.2397504833 13856.9485339478 13976.4188664908 8367.72604201431 6184.93922412311 4773.7528939662 10216.9246295891 3402.60725873214 5578.73529592474 9369.99781710103 1732.29777296156 3986.12157799273 1787.11171908118 3488.76195369179 178.550326579839 0 45.8466234648705 17499 157305 99 7669.84411356283 6755.73382455309 6867.22110529352 4075.06260588376 35367.3422801192 28971.1206709739 24062.2776201896 23946.2883335537 78290.8087860967 1270609.94100026 55917.6069371788 12430.9986903168 17361.9696784465 111944.855250051 51897.0592005583 13988.3016880954 14999.8653869017 8348.41554251194 7473.81360131964 5547.34566840328 10233.784274654 4324.28979560896 5067.2723877108 7040.66151692139 2205.97155680184 3084.17846214724 1653.83404811698 3829.94546365896 194.934947231162 0 44.3792811888592 17412 155470 100 6863.61698029397 6244.8847794828 6069.78080076484 4237.21316501872 33161.1624105246 31368.8297869469 26313.4545701624 21406.6625410664 73964.1935735233 1245426.1085917 66307.1042236991 18994.8348342904 18185.4693687965 107766.972785148 30812.578088134 14308.5354213824 12706.8066488784 7025.94382986704 9086.20057902828 4640.09426075184 8559.78697829216 4478.89576515682 5274.179581647 9914.15443048556 2079.16306077945 8756.7475719449 1786.37088930732 3926.24958042539 291.553707423231 0 45.0945007177458 17703 156020 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 6857.4847873761 6241.01952500351 6120.80501253303 4423.84044076671 33319.976423618 32757.7341607663 23900.7086096537 23370.5066638173 66342.8460059732 1236380.20159964 59868.6269988362 17845.8172933129 16869.6404413867 109843.494746587 33892.9471477471 16135.0859678717 13513.5281041129 7837.59010256872 8061.7554644244 4764.55995888727 9001.66984085865 3482.4287636922 5361.29996541461 8927.84562819549 2113.64195097883 5807.23537880728 1761.14443174415 3491.98379156534 214.684316037413 0 45.9007108793695 17557.6 154244.3 DEV 709.734661490829 527.233884482769 552.952775290474 628.613566048143 3045.06254583777 3271.93358349183 2199.72063772613 2508.5652129998 5249.47769567736 48274.583111688 3597.323171206 2998.34352607074 2579.97525832292 8288.98636577056 6827.64216146951 1551.79071441797 928.46601327438 832.696764600602 827.944473318756 593.404640959624 657.937537928476 505.94808300171 474.798678098204 1202.1752007577 286.541536716083 3559.58209092945 239.157605597918 772.744791556759 68.9293316938316 0 1.6951290837984 153.750121951171 3677.34469012629 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on POR-79_preproc.map (4745025 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 4415.30179555 4056.66819669836 4634.73616620695 3179.80742882506 10354.9187988538 9827.6263970797 12903.3003547554 21610.2977270146 18488.6925600109 520771.279820183 17327.4284310472 15225.5197689987 6806.86927567629 12021.0327716149 11357.5568786634 4406.0268777146 8954.05433669362 4743.24130719914 5234.81097584748 4362.18300294832 4770.81700619964 2654.152075903 2794.33105216409 2435.61456189165 3578.80805585995 1311.31687073694 928.713761098504 203.329684558709 85.5138196019148 0 93.8850010087056 18532 121162 2 3336.16355428162 5516.86969288522 4867.22254565402 2559.65077686208 11133.4614300464 12736.9623790032 10991.9711654733 18228.79263508 19158.0309938302 541932.087064967 22685.9394524366 14679.2078425515 6473.54765914521 12885.4465652872 16116.3908360466 4389.87433311533 9142.83514252347 3927.07714104775 5167.99735254746 5800.39162668876 4252.85030196309 3128.5455433109 2899.48340678809 1983.00854134165 4112.63110609262 1331.37059213371 820.573063426601 154.257372457753 46.5566719398469 0 90.3489727610269 18191 120882 3 4311.93050387989 6327.05651766654 7110.00089206142 2624.82953078699 9563.74086553502 12081.6380586897 10490.6732488514 18066.5550019359 18670.8133347737 475117.71171905 17104.4718179223 12961.2584126171 6778.10719979693 13825.9779523099 9737.32303399202 3513.35417609472 8654.15884127366 4382.43967286834 5298.55502228852 3185.26101480844 5564.4169579826 2679.14026202547 2230.49812833893 2939.02612873781 3357.3135553567 1367.818198807 817.84783550852 243.379168175103 46.7937196465422 0 93.7203339773416 18090 113284 4 3922.32109300413 3986.73366354614 4532.02764804576 3303.94328280956 12100.5381341369 12905.7170939002 12445.397239668 23389.3751606115 17534.5709532696 600627.790619741 21175.7373909373 11769.7188080268 6469.66409578307 14361.4581746003 11069.6041694997 4124.61780008016 9434.41167966151 4839.86949441109 4955.6078933408 6527.3295170881 4976.85689783726 2466.16304295082 2495.56231108892 2567.48844851311 2875.98895926378 1062.65500319929 1169.79267093962 594.839073747988 106.106246947497 0 100.049748056112 18396 123604 5 3408.83465820232 6463.68992728573 6770.39649941946 2927.76044399631 11313.4764636395 13485.1183392723 11409.5335413348 26014.2103983378 18292.2764731494 500964.567214262 25323.2365085116 12660.2699988605 6521.75840038076 14281.7511642211 16345.6046828197 4239.04719735958 9112.22402510014 4872.62231490118 5556.63296848898 5019.09911947103 4512.01485038603 2555.57333302902 2685.31245969568 2437.05079473295 2921.09806673732 1507.96048763784 861.488543100378 296.086927543373 41.9627826976886 0 72.6749836839283 18098 121020 6 3818.78851901741 3408.53511812504 9731.34236481988 3031.4569802755 11674.0099260866 11727.4809600602 10248.3802613005 21258.2523723746 20773.0407051959 543201.464136825 18374.8784911742 14101.9140624798 7168.65877536956 15514.7818286895 16282.9702219564 4681.38550656506 8840.20981620932 4369.95479895219 5065.50842731971 4730.68327931992 4908.18372384826 2938.22893021172 3867.27985562547 2331.59418708657 3044.32558677477 1028.59798921701 1097.08567378349 625.130541528893 41.5809115401844 0 90.6976538756318 18286 127200 7 3624.36506368505 3998.89656844964 2417.7101337066 2134.74708328568 12413.3122481563 12758.3945530696 10995.9646877654 20183.8414271865 19328.9069124032 555504.034656609 19125.274691757 13115.1795820849 6975.48408469027 12212.6627440104 15273.5710454129 4860.43679913403 9098.79792922133 4262.10313547242 5275.10861250221 5698.70700702174 4278.54039282751 2928.64309798977 2343.07583614058 2476.83997648646 2751.42946021241 1156.84720435945 908.061303412229 293.64616154117 63.1928681900111 0 94.421985896741 18123 120738 8 4072.81346864229 4088.98069127301 9554.16030829767 3058.84448777775 9691.58739062065 12468.0134315392 11827.7755010653 19917.7212195679 17824.2650260967 551748.398808488 21953.5317131075 15786.5875994563 6330.79867222934 12793.3368807433 13900.6412229822 4744.66811718395 12131.1864509159 4157.50081107428 5073.43130658167 6340.52840010854 5009.16213900159 2623.46474130674 2619.4906047737 2348.04644020169 2748.85340058966 1113.77426538179 1042.27277134984 200.947040490395 68.1960097768334 0 96.1393878466267 18369 122738 9 4610.87412772542 5890.13775411258 2380.98008231865 2863.48002202389 10603.7610169622 12334.0164891605 11628.5141219689 21695.255831345 18055.550656257 495963.340397591 22736.0060157948 14618.0830109046 6289.03469804914 14579.2251185433 14787.7252420206 4664.66301598257 10696.7575222807 3836.23934793656 4939.71080379267 3354.67399459325 4853.1678830174 2504.02547476706 2237.45824342271 2535.43537819394 3135.08526268291 1141.69699972748 1197.56035705614 308.859819507906 20.0444784580499 0 77.7101559460914 18348 126197 10 2775.19430054827 4425.0169680731 4931.21869285754 2569.95200836342 10317.2084495081 13335.6415067921 11429.3324401679 20973.310849732 17572.4654040595 500991.01167794 21149.0862334322 12132.6186757968 7806.27379913895 13528.6288745827 10437.3001822007 4476.84916020329 9784.90880548631 3766.43857293457 5171.032925162 3577.39697449463 4608.56172613804 2189.3949372774 3136.33987535338 2384.42454777267 2837.46721256567 1473.06998391169 970.357110219576 624.376448492399 52.449880952381 0 85.3304426436512 18370 125755 11 4864.88485582217 5169.47278457004 5175.05115882546 2755.46996578879 7877.69327933656 10926.8419959039 11259.0538359667 17141.1329817469 17098.8298188289 509827.512532659 24193.2465220212 13035.807343214 8610.00895579386 16123.819930489 14819.7498017562 5295.22778354038 11517.8553093921 4536.27566074157 6087.90916537746 5542.99674583305 4753.22492593981 2401.39635726479 2802.14130105516 2245.71544744943 2699.46856999424 997.439023011597 1281.11733576649 610.1139006329 63.4719805194806 0 89.195773909734 18298 121203 12 3846.86802634894 6267.04269354568 2776.75417799255 2891.29035393586 10651.0303847347 11149.090106744 12566.2903543478 20795.2442739827 19921.165367054 544199.874591525 26142.7106007249 11601.8851592755 6999.67098469289 15012.1598468132 13993.418449352 5082.72625237861 9353.17227140817 4741.08212513065 5722.72629389919 6086.57767496858 4739.96325909276 2152.94557261109 2531.49866225689 2604.54449074863 2962.22006758623 1240.23103805863 677.253618625559 212.28615769013 37.9929723739714 0 83.583966832275 18197 121510 13 4115.84005866593 4271.31481831344 7802.11738745053 3873.8540959942 10725.5595047909 12688.5801598864 12228.9923904784 20910.0644383601 19028.2465518408 551231.372543615 21149.9940888836 15195.2631256982 6376.1612941602 13963.6115495281 12136.012089997 4043.12881636652 8661.08265343819 3725.85361430886 5094.06606821908 6033.81081979619 4039.93394700515 2500.61846121623 2420.84815725965 2322.84993932665 3180.07448012229 1286.75090264917 834.456087056508 205.176730609223 59.5036185767637 0 94.4233901336535 18169 127315 14 3828.62222088627 5533.19840825589 4938.2006313136 3146.457104621 10551.6220291334 14003.4539601406 11851.2811173632 20554.5086410806 20619.6871298809 477002.953325935 19689.624679544 17442.3927043201 6314.89464223031 15613.6079261174 14250.8077899744 4364.13913454032 10079.7250735537 3969.96355055237 5133.38320626116 3791.52516862135 5332.56009828609 2818.33135419887 2611.52770993745 2224.8320741244 3043.47201776229 1079.86311081138 946.212760420769 202.184470476626 32.4210217568927 0 76.0435411657503 18323 122438 15 3991.76913352939 4949.3709237552 7172.13562659569 3575.55401203046 10803.4873485568 12852.4853211929 9871.88894182705 21295.6593680525 19935.038843691 517456.662772201 20351.605108669 14458.4158833507 6697.94394036677 12864.9443827179 13012.9676377563 3819.89729876142 9365.94297332336 4523.43306674612 5421.74638735459 4050.51651371647 4572.72026206139 2442.98450374603 2479.23422306767 2661.7453117303 3279.77322169717 1081.50539436596 1084.55173220374 208.566204959693 54.6681327160494 0 88.4100433939776 17903 117990 16 5275.54748829451 5434.21754872126 7224.44474245166 2727.23467353154 10129.1641886692 12914.4946934572 12970.9148167935 21334.2148740808 18852.7757090468 490685.650643267 19258.276900313 13410.397722842 6046.7540219233 13164.488813873 13330.2081935168 4536.45375864225 13045.2857745139 4730.95404933299 4796.30075592456 3791.79242256509 5979.74947052884 2331.80976266204 2501.29713990309 2528.15972566913 3199.37506460451 1318.67872375203 1063.46148403195 176.389572486783 55.7131364924271 0 85.9373966980867 18328 121332 17 4195.2513324283 4294.56750097248 4833.9408483026 3211.90975810047 10914.3156608579 10941.1343546682 11508.6075496881 19999.885131542 21904.0895395293 482909.95902863 24201.2561337741 13504.5357926464 6689.30718300841 19391.0638361023 11547.3160311288 5062.59357264459 11774.162710251 4742.35360115503 5685.16552970441 4830.56559071367 4886.24998286219 2585.36112684481 2497.78441810297 2431.8777070539 2988.62546539298 1384.92638441849 971.429356531179 213.310063174271 65.1888431013432 0 72.9769533095357 18172 119237 18 4082.75891257034 4976.66333733278 7507.778025877 3248.85964262342 11216.0479436347 12671.7456076223 12049.5144670828 19034.3509882809 18670.5849395265 519957.643209199 22333.8487421311 15585.9291536569 7267.73450329734 12889.4589206116 9668.03318968203 5024.80170776475 7903.99282653134 4943.01576221755 5152.78135782366 5288.82876592049 4263.75357234114 2147.19179209791 2563.9401349805 2000.05926045516 3034.62988057849 1175.97887598256 712.821117035264 1101.75970105037 48.175 0 92.2002396830272 18267 119446 19 2928.14950256874 6162.59044288759 2337.90122570736 3071.29603952736 10251.2573323039 10659.9286527327 11173.9724873221 21381.4980276512 18082.1857920613 477443.578965901 18731.8031054165 12680.4974994721 6761.71389530313 15020.4326776846 13960.038665609 4686.45382080033 9108.37321502329 4767.61803985323 5325.71171409426 4966.37583586803 4731.24394775007 2161.82229194297 2625.35485948399 2345.79052358642 3351.19555595472 1278.51386961442 953.735734136082 180.613296339821 62.7423996105447 0 83.031030918776 18084 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4772.01950242251 12784.8814103663 4791.44320230557 5664.88559450933 3568.51009922785 5460.2208352101 2913.26428126851 2478.69860246123 2631.60214448793 3532.00219383066 1220.84192038211 860.148725103494 342.713340626269 37.662938265012 0 84.317173263803 18129 117337 85 3230.38279287551 6884.39859679732 4957.4694269807 1873.27680334314 11434.5777585056 12531.8162173096 8708.30104404407 21155.5025426605 19945.4348907235 530048.673361818 23099.4579665767 16896.9946362574 7373.64626510412 14262.3025628806 14639.0911139531 3685.69191354265 9363.97307606582 4540.06538982341 4998.58539743171 5426.49544544654 5090.37188645988 2663.88019488249 2702.50116929724 2757.54414511601 3309.31734925832 1186.2714732244 1391.63450328644 169.197495812773 45.2034314757183 0 82.1341060982617 18179 122830 86 2947.15466112277 6890.99260171689 2477.15969434529 2441.02492004571 9998.59676812999 12964.8244133076 11672.4926199208 23824.4839180651 24256.0648697071 541016.174301203 19778.0767081627 14894.7940227601 6210.29104285338 15279.150907935 20099.6880746247 4449.22455449382 7980.90871640402 4697.35173776055 5068.59894905609 4174.69866904901 4711.15785650255 2632.7183601693 2478.44903745545 2748.52623897484 2878.7350590938 1316.75300375889 1029.75147038601 288.417499031943 62.4282738095238 0 74.6779952330918 18304 125632 87 4462.72309478814 7154.14442026652 4796.40955744276 2294.70221446364 11959.404959001 11409.3411035616 9015.77121079167 22062.9764028499 23259.7295596718 510253.035866559 23493.937773436 14439.5194664667 7168.23511682593 15499.7060494883 15460.7601132433 5355.63671327004 11521.8635092203 4320.11581511894 4995.4778177171 5273.23384525088 4777.36917637161 2455.00216341312 3097.49207132821 2973.68767924378 2851.32925260929 1443.87574488705 678.236205189932 653.115122092359 58.7308948234448 0 76.1982305062013 18287 128042 88 4099.90653977825 4630.95064619999 2878.36729658772 2862.21006108721 11019.2092810597 11961.8693308083 13496.563842134 21409.1178883986 18274.8595106727 490794.569441643 18449.8395779098 13791.4443932501 6343.63537894034 14002.7326360787 11891.2427146908 4620.82838383958 9004.76560439039 4761.20802925206 5987.95918153784 4133.75846853402 4280.67395575433 1996.17276864959 2522.45798520905 2920.90086119144 2983.4049835786 1405.99766617613 978.09229111358 201.633620073716 50.22790424882 0 85.2252808488844 18581 124141 89 2667.08802248974 4116.22083832082 2962.8593799966 2995.31180430951 12015.4208545212 12855.2757207737 11925.9890456271 19055.5008556938 17694.7379818011 498519.754153205 21981.7736637017 13473.6462982056 6773.3407206705 14111.7933681262 13030.8466574799 5019.34020271021 9525.31373670521 4137.64385089524 5316.10066360607 3611.46439565929 4986.11765107248 2314.281294159 2529.06294818844 2482.61529509001 2797.86078671104 1336.85256516704 888.550599419528 725.065061013676 72.3541666666667 0 83.1988478380123 18298 118601 90 4290.70322854061 5453.25696154308 2726.78060626757 2793.70122533765 10459.99792749 11020.7369209026 9258.14276919077 21402.9264659344 20397.1112695487 500880.707393745 22521.0482148863 13716.9550934132 6812.60379532566 13630.9296462486 13128.3696320541 4688.6354432383 9724.71305329475 4536.14127333575 5225.53662568143 3516.64413538475 4780.34697174119 2217.36831731881 2538.58074935176 2377.66562964852 2941.836116169 1179.23576500176 753.953470510767 148.68360977903 54.864933525256 0 79.5854374509319 18286 121344 91 4312.88475169476 3583.83689077651 7500.30105317508 2951.1486050302 10955.1604127085 12716.8573467686 9558.90559980996 20344.1550031485 16967.146295713 563646.817546119 21071.3766945235 11940.0936836995 6236.1499483188 13940.801399391 17820.776652116 5206.80476544424 8638.78515805202 4683.53446883351 5828.97560418275 3787.62899685079 5065.08349444299 2412.23937722766 3156.40023055719 2475.01650885771 3017.76179242591 1350.83535995603 1222.43070694061 266.058367097313 32.6090409095545 0 96.9725972905322 18111 123579 92 3938.38545729966 4014.08700526544 4364.2983507644 2657.6527469466 11754.4534617486 12248.4557473721 12113.0824313342 22649.4817577952 18813.0210330745 555233.693753657 23806.820528914 17349.2994219946 7256.68898125592 13320.8794051479 14457.2480184244 4343.46640729912 9746.03805436581 4848.1980186114 6237.18301329462 4904.2249112869 4945.91523321963 2650.33659254997 3182.40376929593 2670.52188438008 3458.55790255855 1368.34660404387 933.334719281406 164.87010192446 30.3470953777405 0 84.6873897527098 18262 122176 93 3182.11991775519 5293.28831621599 2318.93381262206 2473.63057752604 10925.6235414165 12029.2288976026 11652.4328806375 19877.4851952516 18830.7916164573 524629.473423729 17185.4625878089 15068.4962305639 7076.52724315472 13345.803399957 12022.2406098772 4112.62335888301 10112.695885131 3790.64674553308 4715.21053098312 3444.54067288428 5944.87773782812 2207.05553732202 2711.52407963945 2386.91079140061 2955.16536835807 1056.11727678454 701.941382655713 179.379498039437 64.6881840353844 0 91.3515620303088 18386 123388 94 3649.22394700249 5913.56665547031 7343.85026985922 2960.56293140758 10041.6703250498 11964.6099598451 10137.4426026453 19278.2025950155 21079.3496929358 501424.845761241 15463.4372732496 14751.8434661225 5963.19500915059 14683.1865604433 11360.8651348489 5050.15342384253 12358.0756338789 4875.05505750359 5157.16348004967 4028.92576019331 4708.87188712037 2382.86147780528 2676.19518425474 2243.74481390998 2947.75139800278 1304.30465902332 935.531311349466 1020.01605627804 32.2300120444072 0 92.7805173068451 18044 119115 95 4231.17556278811 4692.62030740925 5386.73090714394 2509.35811532825 10548.1683581785 12658.4970478505 10819.5165432778 22621.4835295519 19290.0876847974 522701.915267183 19852.0206897944 13612.560016748 6346.49595351437 12032.9589950701 15361.0785490068 5230.27211830693 9811.75740829659 4233.69494961556 5193.90682752702 3208.17905975776 4754.77872828656 2342.49761401617 3160.2545521331 2351.48494813692 3089.39648178811 1193.50471033186 848.048087010817 236.351432797101 51.8611691086691 0 86.1160080512009 18199 119919 96 4276.66196386185 3761.53115664694 7364.15157646947 2689.59093479445 12018.5053268587 9645.10003531644 10793.6397370214 20133.6733576563 19425.9604417093 479862.585611509 19112.6430839071 15540.5349209849 8867.5762168877 13583.6411853739 13432.6039289981 4922.1923907622 8813.70728419092 5347.53710647251 5693.39769710593 5449.92465658456 4781.88506146158 2420.35796398459 2791.08664885065 2702.85308607757 2493.82273051938 1194.98531761855 1006.8135972366 606.911454553949 74.3820038491121 0 86.8396574160475 18097 122215 97 4274.44753358853 5307.90662742597 4935.18351352117 3119.49129326969 11611.3510324527 14095.5344188625 9918.15959146086 19498.1294704868 21241.4431998128 558670.682919329 18099.8700970861 14869.4426836239 6893.63212499798 15075.8011792354 16396.1387892898 4066.71956237885 10729.0147782499 3966.8256130542 5783.35087392538 4115.00935321863 5114.85699620958 2918.07714853199 3372.94523619512 2316.45441407526 3035.91856265057 1210.84975096393 1098.43172213147 303.601193384936 60.6501349904576 0 93.5473170924789 18295 118342 98 4304.7050973719 5191.15277346728 5205.42022126829 2812.55736758411 11117.4752875966 12034.7639803192 12161.7815001454 21709.7018895941 18334.6065092513 504531.432268381 22194.3436386727 13761.6086683764 6102.0403249611 13251.6472581449 9389.23713741879 4725.26113580955 8367.62540276945 4828.9493952458 5546.74033055885 8225.17809334367 4974.56701404863 2639.32584453641 2415.21754341192 2656.09022614371 3391.15235640559 1085.72049868299 576.854522701187 282.437446638997 49.6854070484609 0 86.8570518130654 18386 119987 99 3748.73657304465 4828.30070889541 4711.21770941576 3027.79954719015 11272.1710395446 11703.6842915694 10519.4443084711 21949.6669453736 21851.5297786359 526166.905532312 20426.4346611285 12505.0104226363 6922.78518987492 13225.5221651646 16063.2006885874 4239.50895533885 11196.7068398946 4395.82729454926 5594.25488448466 4706.57623669593 4547.05817189656 2295.56061444683 2642.31269735125 2176.40216498704 3610.8889718583 1564.69061632746 689.082493049102 800.850714279333 58.4665476190477 0 84.1123238613035 18085 121554 100 3195.0922585096 3245.63904188682 5037.07146681984 2081.53339618307 9929.52297655691 13627.4002268977 12002.4939194915 20143.1363322565 18791.2473587306 505193.495234126 21813.5285211495 15569.5132492831 6644.04481363157 13898.1372594095 14926.4527903194 4703.62750817263 8923.71993506098 5181.07758231452 5366.75509734438 5750.45387938071 5336.82741764754 2823.61928887381 3292.39966560988 2840.36768875128 3094.26841723824 1319.08239559541 1010.60156574398 214.957819040981 41.1518820861678 0 82.1754293068774 18077 118796 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 4013.93392559549 5093.35754927986 5035.65943930672 2850.5727905397 10855.3304829143 12173.6927806916 11560.5026848323 20827.1382691847 19516.2842922923 522670.739620498 21006.6433464389 13992.2055825729 6845.85555159086 14185.5068623838 13378.235518315 4532.4935520871 10101.5094663061 4482.41401189142 5271.41081496453 4825.57908582599 4926.34203848931 2545.99342160931 2669.68912949139 2556.00065257627 3154.19605178099 1245.5361151929 937.144661078594 321.635705503052 50.3823260739763 0 86.9436663955859 18243.48 121796.16 DEV 583.684525947654 1288.96557705847 2339.06228875176 376.19883551439 1037.3607135148 932.47709635737 1426.89407498189 1544.24328717769 1472.58121119227 26693.8652914122 2142.51667707773 1695.47708029536 655.999379978085 1452.89065090275 2199.38992948934 398.61310050455 1289.68092116698 405.677814074708 375.115479995149 967.179583687206 401.541051289532 265.777802088859 340.611052235899 266.896481194116 330.269600208211 158.554502328788 177.980356858699 207.125027141832 17.3933101264398 0 6.77804097078102 165.43908123536 3091.70341307183 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on POR-82_preproc.map (5874594 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7015.70256985778 16082.8548302074 10553.0488680487 4292.1415663544 12227.6408071426 15525.5396051505 24274.3905056 27627.5512246907 32474.7795913147 842453.896087137 33107.9327967175 18555.893919588 16093.5056620701 34460.8044318878 20685.8306567761 7782.40128109608 19594.9171721782 9420.21963107302 7598.18192613381 16570.9404790163 8496.35665459083 3790.51217745492 4077.06947186969 3929.41473538889 3022.97736567908 1794.25174043883 1294.55789438718 385.761479109156 63.7049833405767 0 91.4318224787297 20191 138049 2 7506.41207177661 22142.5979759635 12888.1043002116 4999.46469742007 10204.2826903505 12769.1781463788 22314.3048131517 24329.7511945778 36886.8959853246 826763.807842785 29352.4271830336 18264.2074852374 14401.2543119215 30869.1992106351 27586.0671080105 8517.24035099717 23033.2056829421 8694.47203679108 7799.29287307914 28099.3115271287 8002.41733645657 3459.30543904947 4799.54124821128 4108.94817577648 3187.85180218071 2055.96235022436 1220.75630034009 432.572810058691 162.375575037939 0 87.2874130842863 20561 137424 3 5619.7813380781 16526.61644591 10971.5311998814 4290.43800621696 11095.8810155521 16830.030408969 25835.4827774962 25295.1828854826 33597.6762377228 740708.536092873 30994.9953193675 20699.3998731571 14473.6549721625 28514.6846575262 18001.0874074643 8388.21043598207 24119.9038318783 8525.00973196318 5364.54397524052 19058.6700148033 6752.79826886201 4831.82525322898 12606.9161552898 3496.59806133327 2625.40596661439 1585.13810907409 1749.97215082628 460.423887013097 183.882538347766 0 82.2674490856639 19883 136099 4 5283.97668203506 12209.2810522802 9315.33454624379 4535.28444288543 11120.4725904688 15213.9393838028 24177.8020302757 26026.5188964403 29353.558920602 837390.991956074 31836.3599735875 20328.2051530853 14702.97697872 34352.4462646295 27017.1428712356 8751.83795149449 22982.065754243 8720.40597459008 5743.23507026395 26157.4258252645 9465.09911775158 3383.5760698276 6732.36499589726 4098.55649837974 3025.8663252638 1826.11519547111 1708.13877518493 330.322196705493 116.889410728099 0 86.4628606256059 20245 133422 5 5902.58797441601 17183.3061576011 9639.79201116578 4957.13644269968 10244.4555861969 11355.4519940848 21242.8920087444 30163.4725411624 36117.1123958267 819526.789436409 33226.5366042348 22408.4588939183 15952.4808172281 32590.4555305204 22027.9675217714 8260.92046512338 22733.9592029261 7915.38329943402 9982.76197145952 25211.9208765156 6100.78968760301 4358.30917636145 4302.09389214872 3508.09801018284 3844.40186055669 1632.19165547418 1986.71340591833 332.905452652443 137.636918709692 0 82.4022673881709 20251 134133 6 6607.9782470297 18030.0849094411 9302.81718152746 4509.5416188727 10999.054898585 11792.085183702 19506.1878567288 23696.528687887 31813.8894547798 766584.307610304 33635.7528720699 17586.3551153836 13878.8386856705 29546.6333517588 15283.4686315579 7895.09015694265 26448.5049797997 8526.15256637114 7947.13167823606 25487.0801549775 8101.16246996657 4093.7011917945 4513.86203054628 4056.18357421415 3706.4292448488 2262.45380592726 1834.39623166425 403.003382408584 183.201520067291 0 84.2768637553681 20118 135676 7 6356.2927492923 16858.3237136206 13561.9914731132 3709.30303070212 12083.4354906325 12211.8145989825 23406.0428723967 27292.4648892398 33870.7744067356 848699.155980965 32586.8040839398 17764.4571739712 14408.5206437774 29773.3768682338 16898.3443723619 8172.74522887357 22582.0531526315 8354.72832409069 6954.6425777255 27836.7845995977 8596.32812980395 3535.67323705468 3691.20928833217 3256.74901174316 3878.07064826317 1779.27003861668 1677.7127345314 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20020 134991 82 5140.49463611379 15792.4414970846 10482.4863622666 4230.46696174844 9916.56248676162 15361.7453665626 24628.0729835476 24936.4881967445 35856.0697407609 847551.220148124 29423.853889844 24526.575821129 12755.5311012968 28092.502936743 23153.1432345465 8141.40410254182 22146.8670101631 8067.12433210303 5468.74184451631 25944.218773194 8772.08755210163 3303.23400491089 8405.19662839965 3564.32013029397 3286.13828629484 1458.34760765257 1356.33703484404 613.605359161318 188.019435194092 0 88.8359693895119 20284 134803 83 4866.46119202081 16563.6666762297 11563.5399648729 4242.10448687102 10164.9332932913 12963.375338702 23596.2428769473 19974.5118693639 33521.4565800217 753265.456430976 29078.7493599837 17077.3698124361 15222.2938183415 29129.0286729944 21011.0342529734 8434.09644408408 21905.4108498447 7375.75290822483 6467.73585056015 19412.259679666 6492.01191369927 3174.31584662498 3576.83543988647 3428.96572408733 3406.58535211395 1713.65583850119 1412.05418701671 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34855.4376920396 20096.3761385557 14575.4659902322 30833.3981337298 16920.0848565115 8943.90805698707 21786.5170388195 8296.89609833875 7036.62118333876 22366.1227802333 6710.78818179904 3832.70116254762 4240.61713762415 3322.92289025538 3548.06591333133 2264.27146193918 1483.29219466733 408.194051195757 121.672459867871 0 88.0686851080251 20257 141720 94 6078.84128688442 13042.5740864994 9241.24909100601 3911.87672236446 13319.2115794329 13371.557022694 16604.6376464926 24298.8276666484 33031.3391833497 819400.485953814 30779.8681359914 18231.4935645439 14683.8584149956 30811.0484216104 17103.9434102672 8530.41817819423 24151.540732593 8073.72300153395 8912.45529533091 23359.781525751 7248.90105047843 3894.74750800789 5229.56257130769 3715.28745859336 3973.2164591999 1591.06300621032 1586.69252237704 445.812076880585 160.202236635659 0 93.5791688104727 19925 133121 95 5483.37791422808 13457.7717619037 8018.35961265148 4223.49485965807 10681.7084586332 13717.5419435247 23134.8799238418 30216.6615692485 32545.7677253948 795784.404556358 32803.0873981658 17634.2384728935 14764.9352383695 33234.2561659674 27279.1076948398 10483.1403583656 22353.7028318147 8067.06694423916 7824.66028597881 25629.1573610811 8003.42404555676 4249.57598985332 3911.50132213049 3351.60950496434 3276.04865209378 1985.25540159731 996.096997386353 432.47518542892 84.75 0 79.7003529657955 20636 140388 96 4269.25781643859 15286.8473039405 10043.5069372296 4598.97085220692 10504.900142997 14472.6426138331 22602.1571429519 23832.7793948868 29803.7849125814 803535.05052977 34014.5780445834 17213.4391421189 13102.842749819 29476.4829549838 21818.5455308292 10154.1051849621 21743.8595919215 7708.14723097953 6252.75443675584 27811.7910938678 7084.41769998604 4883.82852250474 4486.75302879878 4069.17308969773 3656.05565373474 1967.38773015127 1182.29676302512 451.707975425834 135.95622170182 0 87.6586005033784 20216 132469 97 6415.85473038724 16137.6217139794 8507.75273495151 5088.05668520627 7928.40251370516 10868.1926805449 20680.3665909954 25259.0271629559 33135.9581011793 778797.205133796 31968.4542927446 18657.7245687486 13474.2848550888 30005.0163675422 14625.7591990972 8313.44437380985 24545.1073066361 7300.21467718109 5927.10238975371 22164.6224526859 7266.85391666914 4759.37397856755 4383.29105306813 2631.87606656745 2689.14154360299 1580.59890837992 1524.10760019318 440.74971159337 92.835151147113 0 83.8476615459509 19710 126801 98 4776.02458888051 15532.4886832975 10861.0983612286 4135.09277353095 13016.9724653418 15726.1398686458 20162.3461822718 24499.5683842689 31394.3536406324 899576.15749425 31916.9239512016 20226.9130534714 13371.9133142087 31479.2998662613 29481.2234917385 8466.83000227438 23040.9671756803 6822.71940704708 6568.63829188356 11860.1664283889 7203.8051494988 3933.58472830513 4379.18238198778 2681.14590871518 2638.80846672468 1686.16753398349 1416.93906968259 339.680153144838 145.329832326452 0 96.0147126525333 20213 141070 99 5084.88162343717 15000.4839783235 12874.0349622328 4792.3547987564 10491.6075078091 13424.2955867679 22967.5925422892 24726.5821309227 33106.0613078025 803002.426498036 32415.2946284943 19652.7090224421 13590.7780349292 26588.2880552629 24306.539664798 8073.66052285121 20843.8650296964 8900.95247426461 6179.55506575052 21043.7577776855 8247.96863688094 3846.64242970827 4025.56791715003 3092.10516510858 3074.84515873939 1481.40934264006 1762.34353980311 359.225827743129 219.452689775934 0 91.0117940760155 20524 141243 100 4783.87480173563 15227.7101951165 8872.23585219674 4561.01222226074 10995.5282645269 15141.9793312419 18567.8486413948 24042.477555112 33574.2947060725 747465.522604084 28692.7573438034 22676.1942353143 16583.0221602228 31124.1287201174 20537.9778520106 9799.68849650512 22574.7210644344 8791.65973410969 6639.5072819185 19047.3248172248 8307.83610695998 3916.83168200447 6047.29247314896 3355.25063341771 2962.70584006166 1687.45096258527 1500.4692172929 316.198112771866 178.303089597554 0 85.6428389573763 20441 143025 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5608.0101340459 16124.5880143302 9837.82602277062 4735.00174567699 10836.6403427673 13407.0031706803 21846.1875680927 26561.4201958969 32916.9811804158 814542.546466749 31887.5050031931 19295.8144170981 14196.8546740232 30809.8527547804 19967.5424272295 8067.41557930936 22583.0460765957 8208.60661544321 6894.09835030951 23214.0519152495 7814.68459868286 4057.11461622243 4933.48769068648 3478.2880817403 3411.29685194816 1743.49311266268 1444.0438875408 421.543335264664 133.830525280473 0 87.2653679214451 20278.53 137009.47 DEV 873.695719594356 2310.73256697359 1344.60901959992 542.618377282283 1054.50672229548 1445.15511865824 2684.25920733145 3204.35208110321 2055.91673548724 34796.5751375907 2255.63396922466 2222.66997663429 1108.87131411408 2033.1342698238 4146.31322558809 737.361590977729 1684.05278227053 728.80415144772 1169.67827315718 4188.30942004449 827.00638548569 626.437809307639 1515.80203790929 409.422753217395 419.546015618915 295.169731305354 261.944342682947 70.680993928237 47.3693607794373 0 5.43740625327009 230.973827738123 3972.22018134695 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Testing for significantly different ping-pong signatures (Mann-Whitney U-test) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + group1 (sRNA-POC-47_preproc.map, sRNA-POC-48_preproc.map, sRNA-POC-50_preproc.map, sRNA-POC-53_preproc.map, sRNA-POC-57_preproc.map) vs. group2 (POR-73_preproc.map, POR-79_preproc.map, POR-82_preproc.map) -> Scores for 10nt 5' overlaps are different with significance level: p<0.0001 (Z=4.93). Sorted scores and ranks for group1 1154267.39389492 703 1109529.47575026 700 1098252.09560063 698 1091493.85949196 697 1091468.5093222 696 1080975.6904202 695 1078613.38198681 694 1065060.86387728 693 1064994.14906052 692 1042933.64092708 691 1039119.76827014 690 1038642.78105492 689 1037218.11214491 688 1035547.26852459 687 1034410.98939169 686 1032483.70136964 685 1031758.54119498 684 1028643.03081822 683 1028136.88424291 682 1027536.37935837 681 1023543.56400099 680 1022239.16592789 679 1021648.75818785 678 1021275.56422005 677 1021151.72926459 676 1020606.65189287 675 1020228.02930319 674 1018511.05213193 673 1018350.69349789 672 1015095.92129134 671 1014952.71198858 670 1014229.54432989 669 1013132.80700143 668 1009632.66033113 667 1005831.80569977 666 1004682.73126049 665 1001262.84772178 664 1000840.4959052 663 1000333.01313543 662 998566.061863022 661 998297.467334233 660 998111.430743058 659 997991.524591294 658 997608.709144902 657 997515.730406879 656 996367.952137496 655 996079.157700561 654 995683.939123315 653 995544.486833326 652 995378.334761771 651 995299.993429172 650 994938.180478628 649 994906.904214814 648 994613.662857599 647 993480.454627718 646 992857.774036383 645 990901.732097931 644 987596.823274912 643 987470.264956301 642 987247.965701622 641 987164.37978562 640 987045.731031419 639 986751.69920664 638 986700.253454942 637 986403.49770277 636 985599.384132662 635 983674.621250884 634 983603.045455654 633 983454.200897041 632 983438.077144274 631 982907.934253001 630 977634.87598642 629 977207.177133577 628 976958.753750737 627 976617.702461074 626 975502.813128254 625 974917.941648225 624 974400.065933493 623 973585.753302678 622 973478.691472739 621 973141.294822207 620 973009.957040647 619 972118.112664564 618 971922.985861098 617 971421.992117822 616 969846.830949695 615 969834.038850363 614 969485.97673577 613 969031.668092704 612 967940.262070353 611 967860.808760753 610 967284.041818677 609 967199.043047941 608 965690.595248536 607 964995.846205786 606 964323.490827628 605 964258.571433686 604 962776.307903633 603 962494.210841209 602 961566.026082233 601 961291.951797674 600 960167.805505819 599 960063.175998228 598 959962.124958941 597 959869.918232097 596 958608.380889252 595 958471.984239447 594 957941.982062162 593 957418.169959913 592 957264.717943192 591 956417.096448148 590 956081.507345232 589 955816.502047063 588 955472.621758458 587 955437.488788369 586 952932.588844476 585 952496.839336096 584 950504.986886002 583 949698.010803893 582 949359.171250727 581 949355.613445835 580 949333.223249394 579 948183.301499912 578 948108.400650456 577 947678.691010266 576 946809.829752419 575 946290.804223799 574 945500.102450799 573 945305.253176035 572 945062.755695842 571 944824.240857155 570 944815.18502167 569 944068.737537775 568 943877.780837129 567 942569.477466463 566 942257.668495158 565 942036.379704881 564 942031.011767705 563 941419.617033522 562 941370.01676104 561 941319.591274407 560 941142.512645738 559 937978.293510847 558 937679.051565886 557 936568.591102385 556 934661.166394797 555 934151.452461606 554 933576.797075529 553 932137.194931805 552 930409.220849016 551 928960.460923959 550 928418.538465718 549 927489.526263 548 925339.091468614 547 925039.177785519 546 924252.585952142 545 923148.868166153 544 923108.486819717 543 922903.854317307 542 922789.712180346 541 922545.213830546 540 921110.893966365 539 919308.661753699 538 919259.643480757 537 917960.017665577 536 917686.919003636 535 917115.742231907 534 916420.404290219 533 916273.996660808 532 915759.71272247 531 914813.770120262 530 914451.030668581 529 914103.943603247 528 914053.409661769 527 913925.200900427 526 911881.726228385 525 911573.757740602 524 909539.987892969 523 909291.914242344 522 908740.524085682 521 908543.357222622 520 906883.547875283 519 906058.600642015 518 903603.294191543 517 901637.175945679 516 900420.677800105 515 900115.258645797 514 899763.331293514 513 896131.115681254 511 893347.926304665 510 891889.623188383 509 890611.793554557 508 888793.986367762 507 888747.900648773 506 883844.156324698 504 876442.264463621 502 875684.050671816 501 870343.940403771 500 864394.496539819 496 863824.765229006 495 863256.074562699 494 857816.3902239 488 853934.490209049 486 853616.299051442 485 853391.812082579 484 848938.056248867 481 840978.082969746 471 839100.978494658 468 838280.480895507 467 835010.659655624 464 834623.655214343 462 834323.994116125 461 832440.104886984 455 824622.744770516 445 822211.861823218 441 821758.496907765 440 820001.472048922 436 818851.311531525 433 813548.079816843 428 812401.842194212 426 812258.245286761 425 812129.677034823 424 808055.609013086 417 806466.448500233 415 805152.738532874 412 801936.268315275 409 801040.961741407 408 798009.082798449 404 795822.353201575 402 791859.267345425 397 790806.567758704 395 788057.512970466 393 787505.181490201 392 784522.250191528 389 783465.337190118 388 782116.258859396 387 781629.432494937 386 781505.876044573 384 781421.01909194 383 780179.557208068 382 780092.524315736 381 779453.148690826 379 778696.214124187 377 777810.99003162 373 776250.168582746 372 772568.600575967 369 771801.16603989 368 771740.700101036 367 771080.063678759 365 770380.772626394 364 770104.752750989 363 769707.24990323 361 768111.714715075 360 767805.013987081 359 766155.875213023 355 766127.095266165 354 765243.964452346 353 764658.456015793 352 764281.576956084 351 764206.554287399 350 764052.89868808 349 762828.965807855 348 762664.336293227 347 762445.167134895 346 762371.286679421 345 760628.135298832 344 756208.390992139 341 754241.915896588 339 752134.459128546 336 751982.833671493 335 751502.991165173 334 746745.834632306 331 746427.894748183 330 744027.215758626 329 742798.347160918 328 741976.24168401 327 740682.111982262 325 740654.96357311 324 738260.873910811 323 738005.337652731 322 734310.462157769 320 734080.098228957 319 733783.161475847 318 733032.590740394 317 732682.491483861 316 732517.354827929 315 732510.553250042 314 729641.919041967 313 728016.101304724 312 725185.061237226 311 713349.582367812 310 706562.154103271 309 701508.790726115 308 700802.460149748 307 695924.023208991 306 693177.752953141 305 677885.09134454 304 672934.138042622 303 671081.065827983 302 640415.19956208 301 622790.671449227 300 614561.266546625 299 603670.975092512 298 602223.825874659 297 597939.656943727 295 595793.855073354 294 588305.094011019 293 587576.670972081 292 587530.770471356 291 585853.623813835 289 583626.242155537 288 583138.186960982 287 580175.886276744 286 579651.975548112 285 578141.574686522 284 578050.851619374 283 577130.640110507 282 575302.325540965 281 575037.002974475 280 574110.158118535 279 572799.237132963 278 571296.452954188 276 570621.665804048 275 570385.85608664 274 570165.633059344 273 570114.039246712 272 569901.517345088 271 569113.369834931 270 568770.31917149 269 568292.325691151 268 566542.616361954 266 566356.326924707 265 564849.733778853 264 563748.681679384 263 562906.386903918 261 562201.436612078 259 560692.387102589 257 560173.20152872 256 556285.6637204 252 555716.632302685 251 555555.369683153 250 554781.034507849 246 554215.580341943 245 553175.482466083 244 552961.896610187 243 550322.263826675 239 548873.997040135 236 548573.666901806 235 547830.727694776 234 547251.515731484 233 547055.056589897 232 543838.181005462 224 543428.618403929 223 542645.746803081 221 540270.568190312 217 540103.930423992 216 539954.633242804 215 539330.795871459 213 538634.280240929 211 537617.534018985 209 536996.331003368 208 536906.483979103 207 536808.751144041 206 536375.284969655 205 534851.533188117 202 533969.99053797 200 533507.284548885 199 531420.258427748 195 529215.676742026 191 527137.099243815 189 525362.449169167 187 524260.993300473 183 523847.748199585 182 523294.929530813 181 521078.595681921 176 520396.939823753 174 519406.981609076 171 518613.818409837 169 517899.575820455 166 515507.977124531 162 514535.674698371 161 514213.857495314 160 513631.845153974 158 513566.425210223 157 511997.160611578 154 510921.061611458 152 508360.447682766 146 507148.464302663 144 506516.665262599 141 503915.778819105 134 502910.504030921 133 499712.954947481 128 498949.741250739 127 498414.406554889 125 496056.469718183 122 492432.962117181 119 483338.492490973 114 482433.202922597 111 478772.105954333 108 475398.578823056 103 466838.308251965 100 459190.512496609 99 438309.69262494 98 437645.874430854 97 431116.643243126 96 427749.485446231 95 427708.027669612 94 427375.298648647 93 426475.366349594 92 425489.511672286 91 423230.314395898 90 421762.275899404 89 418801.824827811 88 414430.418568255 87 413896.432017262 86 413662.9818959 85 413220.807734367 84 413020.641980809 83 412620.217365553 82 412147.2175772 81 411326.62427814 80 409643.65592662 79 409312.644751691 78 408821.589386342 77 407207.511264363 76 406661.657082924 75 406470.735318619 74 406035.735019 73 405362.880615529 72 404439.750646979 71 404241.187460877 70 403543.233085274 69 403379.682376166 68 402432.383694366 67 401711.382259663 66 398473.9155004 65 398234.592331029 64 397667.695455287 63 396817.375129475 62 396378.245779791 61 396134.658483767 60 395319.104180437 59 394888.908629202 58 393167.871991677 57 392545.601591199 56 391604.345821569 55 388233.633074249 54 386971.782200967 53 385054.280787402 52 385033.468642193 51 384972.026666844 50 383552.79423556 49 383063.502150967 48 382839.876793332 47 382478.303837409 46 381893.463339752 45 381127.510512858 44 380989.359813792 43 380819.145763086 42 380626.72722635 41 378399.418924937 40 378194.286570095 39 378003.648078271 38 377653.282938826 37 377631.440433401 36 374395.288068784 35 373625.502716978 34 372196.087298978 33 371523.603661528 32 370720.339813431 31 370389.157406381 30 369949.580179311 29 369764.546237203 28 369707.557625838 27 368811.358843674 26 367730.122014047 25 366248.416126337 24 365789.724133784 23 365622.211764478 22 365106.126468186 21 364148.975525355 20 363185.183667433 19 362856.471217028 18 361699.855229354 17 360756.004902022 16 359292.595207225 15 358461.884906496 14 357605.635163437 13 356998.082903674 12 355111.279961673 11 355109.004054574 10 355066.374978702 9 354783.592947497 8 353753.753963946 7 353519.72959479 6 349322.067301802 5 344238.245367824 4 343087.45368797 3 342344.549779214 2 318976.789398712 1 Sorted scores and ranks for group2 1330158.3479775 800 1326672.51182689 799 1326294.40848943 798 1324655.64368469 797 1322461.78950314 796 1318418.87020406 795 1315360.19277171 794 1315121.45609755 793 1310409.08413898 792 1307646.16830954 791 1307627.9107723 790 1304767.572857 789 1299580.96526612 788 1293026.26663547 787 1291562.43989626 786 1290716.48202903 785 1290169.1750711 784 1285653.30190346 783 1278158.64233018 782 1276564.49495672 781 1276268.11421399 780 1272904.25325753 779 1271422.88370315 778 1270609.94100026 777 1269797.54783772 776 1269539.03143562 775 1268857.50207728 774 1265757.36544814 773 1264111.97530244 772 1263747.52973019 771 1263368.97545653 770 1261787.56876517 769 1260067.99018791 768 1259709.37079625 767 1256632.53498621 766 1255776.76179298 765 1255758.39454478 764 1255370.04189112 763 1248837.7827997 762 1247566.58645232 761 1245933.09232139 760 1245536.84665416 759 1245426.1085917 758 1244507.29201189 757 1244327.7036561 756 1240905.70340309 755 1237979.98527733 754 1236415.57913675 753 1236196.81815441 752 1235362.76699869 751 1233968.28621749 750 1231776.90211907 749 1228354.53450489 748 1227171.36123622 747 1227163.39191517 746 1226376.49039551 745 1223279.00069885 744 1222709.65705258 743 1221337.86621069 742 1221083.01028862 741 1220274.67726924 740 1218255.06143867 739 1215830.10117762 738 1215820.05624256 737 1215499.5647818 736 1214999.69879073 735 1212301.24416313 734 1212074.70402719 733 1211807.94425707 732 1209827.27821758 731 1209234.5345537 730 1208647.657025 729 1207517.42127864 728 1207031.41197589 727 1204429.7443567 726 1203472.30955947 725 1202745.50833386 724 1202297.58591811 723 1198900.16046901 722 1198375.67401087 721 1196752.87480503 720 1192281.15445936 719 1191773.40671532 718 1190237.54758351 717 1185721.18709895 716 1184044.06143811 715 1183727.09229821 714 1182738.07322506 713 1181880.48523824 712 1179935.57660111 711 1176069.15725504 710 1171239.89276537 709 1169107.83871725 708 1167756.98657573 707 1161207.65356952 706 1160713.04979705 705 1158705.64063771 704 1134884.24244514 702 1132782.59084009 701 1098389.03680631 699 899576.15749425 512 886317.540274934 505 878795.012717576 503 870323.454914473 499 867172.383983281 498 865094.657832686 497 862364.421257436 493 861929.405982098 492 861795.926907626 491 860956.904688139 490 859963.182207392 489 857219.562438967 487 851118.653128136 483 850224.641377708 482 848699.155980965 480 847551.220148124 479 847311.881439717 478 846794.547750118 477 845184.011257283 476 844863.650335081 475 844630.914066645 474 844148.98750479 473 842453.896087137 472 840473.465428238 470 839945.715693101 469 837390.991956074 466 835896.843030137 465 834990.509420601 463 834261.431923597 460 833670.355392054 459 833515.935030365 458 833391.210101564 457 833152.516097438 456 832326.198795485 454 831582.893433622 453 830865.02481632 452 829898.295046213 451 829795.638560562 450 828918.055353017 449 828810.721071803 448 828769.455946857 447 826763.807842785 446 824352.029619515 444 823682.565557433 443 822584.961569516 442 821476.18941436 439 821347.830425939 438 821042.996079151 437 819526.789436409 435 819400.485953814 434 818719.518593357 432 817839.395529089 431 816789.307704373 430 813970.855584668 429 813262.752224486 427 811757.675391589 423 810952.589646429 422 810434.188430538 421 810295.17365383 420 808465.399831234 419 808063.268571446 418 807819.302075511 416 805894.928718018 414 805646.141821052 413 803535.05052977 411 803002.426498036 410 800690.34791689 407 800504.534729272 406 800373.409278807 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