Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-140_preproc.map (7898972 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7344.19900759328 9554.62794889149 10068.2137019766 13929.1721471451 28111.7816134261 19184.0534223279 37880.5573657569 69070.3708793474 111577.50196225 1774209.68973882 119955.257444674 149348.605462194 35739.0662499566 28869.0068374168 29946.6779194349 12772.8549777938 19499.5611150387 13005.3465437595 20964.2882138154 11841.1464918489 8921.39763550138 8632.18183619604 8575.02297713423 6018.69212865567 5655.34771087239 6163.22755153894 2503.19880793506 1612.77237115959 449.728596640694 0 42.4391930773633 53904 260929 2 6953.98524990333 8195.54286648235 10368.6374179578 14722.5046739099 24741.4469310812 21379.2751806204 35938.2176672078 65793.6777852503 121412.093817456 1734396.82766259 115905.041458892 136666.350966767 32257.1123704011 28630.3969381085 28897.312239673 14222.7640884218 20431.0586738769 12948.2934067446 24835.9494499787 12814.955816334 8501.98455129903 7272.20712930143 9423.76602700271 5500.27303022404 7275.84030274431 6116.68074481507 2933.45240362956 1827.0941432882 772.463694317882 0 42.9236184204574 53527 258632 3 8010.8512148919 8761.0529757406 10636.865518486 15410.104006279 24339.6058813997 19919.3043889557 38316.3203524756 52700.1865912985 110531.234045117 1661379.63299767 105274.179503022 130208.863024277 34344.2145003734 26864.0691891325 35667.0603406743 13876.6785536727 19397.4852748473 12855.251577181 29337.5184262903 12978.6242562744 10424.9914251125 8101.51329880555 7941.47605494722 4941.66147447121 7515.97545527719 7896.84072428765 3145.92252393554 1705.95982614089 679.108209906319 0 45.2929254773114 54222 265228 4 7756.0567412197 6841.14175521144 11063.8152660013 14155.7154231063 26511.5981975104 19432.2110287054 34446.9453514187 54023.1581326157 98329.8041694467 1680997.77648587 107127.99968772 135941.94384271 32588.7475646541 28235.706556666 29563.5043296981 13437.075398889 19127.5914491279 12356.0520226719 25846.646276236 12315.9636396528 10543.7346435246 7136.03747241461 9029.26759332536 6309.7854912076 8022.7595969602 6883.50890871308 3473.85337114534 1590.86520607404 669.555092362743 0 45.802295250942 54045 263782 5 8700.5832433407 9990.59744869708 11735.3955777745 14747.9609979703 29988.8714348667 20184.0827136278 38650.5066996122 68321.4726331761 93412.9166036898 1741898.95706128 118126.713781058 127893.383310213 37354.728800066 27204.3430776445 27048.2361737011 14077.0512217035 21783.038722631 13799.9185452184 23832.5734818496 11567.6192905623 10223.0313251468 8720.39215661597 8568.40836356183 5922.20108695852 6542.14281496973 7345.35821136361 2888.16360756435 1457.28476003188 595.651362097537 0 47.647220463601 54080 260509 6 7698.50804742937 9763.61070889731 10450.4546684253 12971.7062234609 29020.6275357917 21005.5177941264 38411.4723573413 67326.1552206093 135268.646909559 1755688.11030097 116682.383732994 137335.146257136 35696.1654611593 29327.4924752441 31029.1416079216 14205.1667511208 20961.529284363 12588.059929803 23841.9867805606 12908.3481854411 9314.985689401 8871.75562247863 9507.60803852785 5237.92032073977 9412.59676990372 6207.89059460511 4643.24261695277 1250.88548806588 710.942052153038 0 41.66307980041 53912 255115 7 7769.97708151681 8957.13752605436 10501.1894183052 12521.0494494395 27220.5708829933 19328.1535177738 35126.5309116405 55086.1051184731 100737.079372672 1788025.76644439 119677.377879112 131009.560833989 36307.9050352357 28635.3814748137 28026.1253997646 14093.2078582312 19691.7070094301 12317.6050145111 24439.4361392638 13414.1855323382 8394.2576992781 7705.8712287597 8003.21735948273 4716.23401684734 6482.3020528398 8043.12707854589 3070.8619184124 1673.35643372055 381.311361632773 0 47.594061786516 53993 260986 8 7272.44115721295 8917.7405885748 10824.3388434751 13726.0528152728 27107.7974316411 22450.9042698541 35545.5198920999 61778.6074271492 109509.043004116 1773925.31381912 123452.544465266 111296.626750059 35243.0664183137 25987.8384093049 30276.7177606876 13563.0644385892 20916.2431211489 12948.0430490259 28152.3554119001 10953.8683862995 9515.1179621246 8156.48981586164 9605.38921720708 6966.53148413471 7381.66699683609 5703.08251859164 2268.95160606239 1740.61625643838 477.814463246953 0 48.4720371806695 53420 259185 9 8713.05796390458 10208.7978468679 10237.5445569317 16034.1661100974 26795.4122216399 21392.9789471386 39649.8203879451 62574.3772169709 122870.116058722 1578974.52754768 109929.024607611 141009.376475609 34975.0934172067 26750.4449570605 29873.3666432723 12975.8331413438 20072.336461934 13532.0433513885 22738.5086035086 12467.0390932347 9354.92944180911 6875.11071469241 11185.3753178716 6910.47278870618 6965.02899684333 6558.5177151331 2941.85579945804 1556.08337229958 755.568586896619 0 39.0870808865478 53667 258290 10 6490.47097130086 7664.5863036786 10298.2000381083 14847.5130593686 26698.0690461453 22029.3353584737 35801.9323458294 63994.2298497224 105452.849532252 1629588.91184397 114601.235475631 123924.346828586 35264.9739219012 30034.0171423675 28130.8009575369 14263.461026472 23176.8891885814 13865.7481527268 26460.9023330251 10893.2406562468 10623.5752015628 8087.1514636124 9987.60117476793 6341.22257227321 7284.32745532793 6016.41350864856 4062.11188425557 1496.59445612309 547.550593572427 0 44.3535388906742 53508 258256 11 7332.33729833661 8295.02444595018 10959.6602384644 14652.2513519702 27839.9167799667 19812.6591413287 37819.0476955745 58941.2435864491 93652.1461643097 1746487.17058068 113715.723094182 136782.613636187 33671.8487953902 27484.3860200643 32878.3832499025 12983.8961509113 20472.5011696776 12402.5895187969 20839.5155634641 12037.52858746 8686.78556419738 8119.67935949078 10293.7145738983 6162.06045527259 6159.19040628457 5648.81295250074 2418.12062095595 1533.13003579469 762.475541584215 0 46.8919449861969 53661 259486 12 8396.70356613958 7924.54747624085 11797.3231718732 14762.0608051752 25043.3008854205 20741.462970005 36848.3733500677 53066.4325321732 103843.584925729 1540351.3626459 102211.429888156 126962.206694076 34746.8153669326 28635.7592268646 32147.1814242737 13529.1271701649 21688.3490952902 12517.686226916 22854.494690277 11405.4650589248 8613.07815163311 8980.35795712323 9490.5680664693 6445.04357464308 8016.43583054789 6219.25602051134 3258.79389961494 1428.01870781109 510.95072059764 0 43.6100062813274 53845 259954 13 7096.69263548661 7701.70904536969 10225.1965464605 14083.5119899883 25088.0061611201 19081.0468389145 36407.5962255805 57215.2192834362 96415.791132948 1681189.63892381 108246.598617524 128035.671546686 33368.3918584315 27192.9909208617 28740.4140551183 13815.8659216382 19742.4967982657 12730.4681977816 24624.1802375523 12616.8135923655 8374.95379265779 7887.11247799671 11946.0196138722 7936.33105502683 9296.21035291559 5589.68484659185 5703.72551863123 1509.66589096459 985.799076941584 0 47.1979684084658 53331 259697 14 6257.72212224538 8812.00892435293 9780.47436400503 14359.9628225581 26714.4371855893 17946.8202497307 36096.5728565336 53572.8004216245 112605.025841849 1692877.34788892 125249.53279424 144075.126863022 33369.3675055879 30267.0091447933 32401.0024263174 13899.531563971 20921.2000282676 14086.5885828638 22851.4834669201 12116.893896083 10007.6642614077 8156.24826910395 8494.87396727906 5706.85830556421 8380.58472352144 6332.77088150612 3277.73421731388 1533.97944431361 547.02687744531 0 41.0398978067003 54257 257223 15 7484.46762194399 9895.30097777506 10319.8632657432 13983.4275140238 27156.6737309093 20062.1722365881 37493.0721299632 63230.3123959871 109128.16407396 1772173.48845505 118958.93723967 126376.905285765 33256.1698561495 26533.3912915398 31147.7117481916 13086.2797662016 17191.7985939674 10952.3312105884 20283.4813440055 10985.5342481646 9270.02155247876 7869.72501652685 10688.2522363767 5929.96041621283 6819.41041676754 6414.90457382266 2758.08682414656 1549.4080187953 552.854976693233 0 46.382839919647 53901 262947 16 8162.0468238829 7948.18494098157 10775.5889590266 14549.9431813118 25651.4851164892 19365.0598067175 36584.0617061876 56578.1056590609 92978.1399756317 1650097.73635843 113816.18013937 130593.839956231 32374.8950093214 29968.9263187061 31687.6476195215 16411.8528670651 18015.2934547776 14108.4152868752 29523.4273454708 13641.9265849553 8548.54196691383 7506.25266925256 10084.294781738 5814.02401286368 8480.01727655655 8128.15302183292 5415.74925346943 1578.27238054069 553.112287108705 0 45.8740230680905 53879 257703 17 7940.81001979251 10632.4723727291 10946.0587575211 13609.4335053574 25475.2900307712 19862.4271058831 38614.7907745294 61505.8901945269 98703.2718431528 1797400.22689009 113782.507942773 128772.79803308 35610.6617329577 31609.1045393894 31447.7231453554 12717.4616921043 21356.160826607 12851.0181657094 24145.2682682852 12209.5050179841 9055.06943220809 6951.83992206038 9200.3631777731 6091.87281829657 8101.62538973782 6474.90851668203 3397.14285187679 1583.94389198884 762.717154831163 0 49.338568440402 53896 259803 18 7401.67935871657 9983.89957736344 12041.8803894781 14919.2159911095 25335.089400596 21640.018675846 38856.9654132851 57706.2213653247 100721.40071014 1708994.88365559 114103.095000397 138064.127791799 33861.1021842828 27612.0472720631 34540.9597249876 14649.6558958005 21176.4527779642 12873.3849562736 26346.2777256179 13341.5360001386 8960.80088762374 8582.28353546255 8735.54440656603 6442.16087154193 6399.9231387679 6345.23656337646 3212.63671969137 1677.54462800461 904.752138869025 0 45.3213711530196 54001 257306 19 7040.38183975278 8729.92514019547 10946.4091739333 15583.7683758554 25777.6210890552 19530.5544134725 37179.1000816547 56911.0289575311 83646.1064552773 1646393.50103067 106805.718338794 134403.55353676 32711.3005934745 28192.1922540575 28510.5339971789 14390.7378533099 21196.847529913 14950.7533534472 23407.763291826 13594.1027152634 9472.87410178938 9519.83799047094 12069.4284061266 6210.76722718207 9847.94830011812 5676.31790708297 4929.1258505539 1687.8522247519 574.45958014293 0 46.9800327409943 53773 256469 20 9147.24773358983 10227.7293321855 10377.4471272527 15399.9502493048 28171.0370662945 19241.496617475 34760.7196027025 50225.3909548592 103214.985058127 1608063.93442791 105439.007083313 138326.937359374 33832.7815054357 28280.3899005591 26785.6233756552 12629.2939929563 18173.8543549642 13454.1708333394 24719.8630403668 11443.5440959719 9067.21139369241 7487.84160536088 8442.58753462113 4782.56737415779 5605.65884449504 5098.13867871925 2299.00296709285 1403.0546199486 744.818103738933 0 43.264637750335 53610 259373 21 7935.20206512213 8503.46095799711 10460.142247627 12746.2588030717 29248.6442556784 18216.6058247807 37279.4002900416 58639.1106382021 102593.710644716 1783267.39310619 120390.462723712 132130.555792766 32739.436125517 25941.7006965757 27380.3728480375 13095.3267017282 20640.4425942612 13361.8760047222 22310.4527111084 12051.8366751838 9707.37559950704 8119.81017723713 8293.73218472029 5514.35999090483 8931.23021140919 5926.23201722074 4001.73362707129 1353.87174992527 481.431965555964 0 46.662295894029 53746 256170 22 6667.98895529864 8307.54240067656 10461.5665514794 12511.3563138257 28972.7417599965 18249.9974582758 34265.4322369361 60043.7777546132 120991.803513619 1662846.47657585 123299.274305312 137916.829925409 35662.0387520637 25663.6609624565 27724.7162414202 15093.3628455785 20551.5967412216 14214.5894623957 26557.908571768 12967.8923143587 10999.5495375726 7314.86239239464 11065.9260180957 6289.05710616852 6186.90516433837 5579.9645492022 2281.70309062161 1747.63331261115 588.954035522359 0 40.3405888089569 53513 259394 23 7239.58053580304 6880.22024199507 10707.9738190347 14325.5516769789 26024.4767987645 17705.333763607 38737.8654270949 60281.9051442751 94356.0458219104 1775514.66764349 117573.101905033 125045.862598964 33080.8560213952 26422.00873475 26812.3982462824 14450.2165143286 18038.6490869013 14466.3593728216 25271.5396524701 11186.3465171427 8548.00168523008 8022.33243647856 9426.89569017492 5431.69438746801 6810.7435000846 7171.0282886919 3639.38564476525 1791.5799721361 443.386619972136 0 48.9680359636134 53741 255250 24 7449.08099774067 9337.28921641058 11827.8355900062 15842.8503735744 26291.933135562 21232.4032761012 36441.8831714751 57223.3528655956 112793.15380143 1694311.04985389 115175.856210623 109264.890895672 35039.458176334 30502.7075212752 25450.5131971703 13346.0063307401 18927.6395506787 13867.8735855479 26335.7840035429 15002.1002565408 8474.90801526162 6455.83156996155 10504.8735422756 6207.11598470478 6010.90734891345 7444.76737888235 2997.46417293274 1255.28046985672 695.809206823182 0 47.8777658255147 53952 263606 25 8500.85583554936 8215.11465507817 11436.1762550244 14939.9343681503 25102.352804559 19845.3019346665 38785.9637426216 54760.4680285466 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12955.6899778527 20804.6059416928 13383.0679875067 23413.3422820922 12475.8926210296 9190.1896209366 7164.21983278065 9910.28015738442 8300.20768201022 7618.21879917525 7815.83475403047 3382.03281446869 1449.69816708434 689.164266496748 0 45.4281581818782 53911 257734 96 7890.18855361294 7947.18046510767 11377.7715711533 13621.6813008992 26201.0357226386 20207.5711315667 35754.5276822802 60391.5036336344 116448.201213455 1724104.83752902 113128.761544295 140205.151879523 38178.5118767464 28646.5969434071 35086.6948177506 13447.4866525508 20181.8758531843 13794.8193544916 24241.3701711597 11780.3200079272 9360.93272551226 7760.14431549052 8378.33457287883 5898.72576708015 6416.84648144756 7506.32619504394 3106.91794562173 1814.39548805072 467.515932281497 0 43.2974078618741 53493 256664 97 9019.68599505142 7938.31862852504 11442.5744897024 13675.5936486343 29060.5594634924 21463.815938087 39220.145052897 55933.5307420031 97556.4650250263 1770302.16742649 118632.485377359 127684.772898856 30931.174610928 27710.3236892997 26133.2970013306 11059.4194495172 19245.9149502489 12447.0218581839 25242.5267133738 14163.1650540785 8921.69406784142 8405.01019929328 8191.39263679604 4852.91640467554 6014.14697951252 7195.79704167336 2709.76161228625 1294.29447161103 743.398284648467 0 48.2379973562657 53321 255757 98 8184.1074498695 8012.83101639836 11397.803236937 13264.1757523695 24939.5065057292 19756.888120296 37904.9948336298 51833.3901720517 102363.433367704 1719386.32803173 111501.08913709 115580.538271913 34057.0676717034 25407.3589323797 30344.1789391535 13290.1139659493 19913.6903616302 13225.7010173819 24481.7367429651 13070.8684764786 8854.01303699144 8018.70644583177 8947.93471956585 5677.77999796471 6169.60325219145 5620.21440021678 2583.31354929644 1733.1563668438 532.871778997223 0 49.642874291664 53775 260533 99 7306.1634117109 8413.96475745857 10885.7772088071 13026.2316578328 23126.1467705561 19271.5446917081 34554.4493876322 53565.2099343311 113029.009433074 1711643.38879883 110538.778360717 133787.40830014 35549.9843898228 28407.2474677931 27986.4775558349 12613.5760561752 20848.4196318373 13489.4386614724 25401.0256888737 12752.2439531925 11502.2420311127 7585.91257162864 9082.67686824721 6216.58065104991 6519.58946671409 6749.93178476722 2963.15580816472 1403.62168879338 550.300956202734 0 44.7656721097837 53564 254150 100 7454.45429087431 8370.75598825261 12794.2756003848 14096.6460001162 25839.0895072203 21427.4613803214 37335.5301165781 56648.4120445761 111384.16531471 1792928.31318977 117701.607487056 119117.10819057 34461.1669031891 27630.5371734629 26406.657833279 13820.8375097595 19926.3121540795 13137.0576236326 24067.212896475 12457.4281228529 8228.68026905886 7067.85226675656 10914.7141067801 7139.58133472505 6833.12046534269 8374.64839884632 3758.4414830801 1797.87677966318 802.582562941844 0 48.6799262506654 53813 263891 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 7687.09413139014 8673.17237952825 11082.2110192901 14354.9673919161 26797.562406392 20370.1768877432 36892.6968628949 58637.987600435 100357.00304638 1708755.19467126 116133.407912363 133178.299695755 34054.8120774112 28575.0554403324 29901.7000115487 13673.1255513557 19919.9930534731 13097.3343328709 24254.1378367715 12566.968254734 9181.20025291687 7893.34797779992 9580.21293714654 6216.90257249442 7259.12975897672 6655.82099957338 3275.99918365534 1525.98993265422 657.137570210966 0 45.5294464423446 53793.84 259236.93 DEV 608.236565897121 871.283670223808 654.690977544641 1192.41411145664 1487.74421487993 1186.84520531777 1590.85651344258 4885.96439847267 10390.5792785095 61616.8067503602 5163.67679281753 9536.48612519782 1618.68694404332 1457.54578119806 2691.69531155485 982.799033377549 1246.28954190093 908.692303365263 2119.83326671009 1019.53892545652 814.691376634633 787.129723443205 941.624502802597 813.106603384401 977.688969354795 848.448283077063 747.447689512579 174.280655970941 157.65028351858 0 2.45680377671887 325.532078910819 2915.49459013389 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-145_preproc.map (8414719 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 38124.5584957518 10057.0183753722 11433.4889617406 11738.3006153328 31562.911852527 31576.7254965659 51437.957829033 124262.124050342 147510.424661382 2452108.89752804 174012.478502065 56294.3872419822 124073.61916524 26046.6292561669 46305.8239703522 20556.9564893859 20998.2286657839 70474.5084988278 25546.7584179135 18150.2686468912 19005.3131221081 6972.36921530757 12572.1341053481 9668.88327990828 8848.36977645628 10589.1567384753 3875.61910996872 1761.41719840146 358.492597273819 0 49.1536137199023 63995 318296 2 47458.2519065189 8979.34365655817 12547.4642553193 12658.5541774792 31171.9979926256 31950.4289529312 51438.6728377383 141275.725454842 118803.140748963 2397741.06365725 164949.283173241 54924.1931176831 128823.334485168 23647.0732405497 46091.4741271746 18653.6824327734 21036.2648508752 80083.7897709774 27333.6303002428 16157.1417255485 22513.6646414695 7726.67709880424 11558.1605423355 10083.6627678399 7840.62504151914 9895.47941150187 4078.56612162323 1515.62507756948 287.01212918261 0 49.6167331773789 64790 324682 3 41597.7147607066 9358.20314024323 13707.94130418 12270.5302809912 35434.5176686832 30858.2198173603 54101.440383916 131007.16557681 140425.025254532 2664187.63402009 156668.817121085 57068.8246953767 126283.495841309 25581.0385857566 49367.2869338966 21421.3009885339 20377.85269358 71403.211268842 23479.206909783 18462.1939371703 21577.105306852 7114.43755905262 13233.5578864342 10785.2043777147 8988.48548922436 8531.77407694999 5306.72565291612 1458.01967488192 368.021835886013 0 56.1232065574982 64733 323524 4 48050.478425454 10218.5034406498 17908.2607444527 11851.1436582914 32369.4290355867 29109.2958417598 52499.9115073478 130112.495607866 129814.15158951 2479585.24750417 158819.10721976 57523.4685405518 127446.666080099 24718.6934017053 44233.9738297926 19846.203050023 20674.4879431212 71628.0051517798 23426.8791542707 17814.6614647899 20201.1490179225 8205.78731590143 12351.810218162 9690.76796510572 8245.1530426915 9897.18807847546 4121.09807329095 1711.06371995045 423.271686370159 0 52.967902299025 64810 320242 5 39227.6977056438 9942.92635437188 15102.1504781635 13819.0514707626 30127.1090160331 30844.3931357953 52168.376304769 133296.358632042 148674.173799085 2492789.98530953 155518.606389758 60935.1872778123 125803.615792474 26488.6366661307 46432.811622369 21097.9232386506 23454.8671090567 71478.8473090063 27523.4756138103 17616.5171236815 19412.3969921893 8207.21227246216 11988.4538465183 9937.86237612605 8633.09249652493 9367.86432535648 4571.35125198089 1703.21703924256 451.797506200263 0 51.6680133734419 64701 322804 6 44942.0094199624 10190.0540332172 14215.316850745 11804.0197756144 32019.0865939598 30873.4915087151 55923.6938326838 130183.938819736 139904.635127252 2589857.02973306 169478.241176632 57098.6863759341 121571.478289763 23842.0523987303 47088.891378446 18945.0092758692 19615.095892136 74737.5561083892 25219.91552139 16774.7962862585 21152.6507716552 6572.84225570255 12086.7270581798 10530.6662020542 8219.35825933461 8039.00494567775 5010.4441142218 1793.89473443105 304.427670282935 0 52.9924495885943 64705 327981 7 44974.3648862222 9092.23102007864 13686.0219885072 12642.0310601209 36040.5121050802 29779.7065638526 57895.2832893546 136456.086586286 131733.036208059 2451654.14274299 176781.460785731 56986.4052950446 121732.825155305 27246.9645237544 47397.6753400824 20603.8229925198 19792.973516359 68352.0797734883 23766.7396673017 17618.2538297394 23016.9207215738 8494.65436775163 12525.1905714502 9315.62589074687 8996.31770975159 9247.83922986628 4424.9049980634 1905.27421997177 434.117465559359 0 49.6130887964214 64566 319494 8 52661.212505326 9304.39791474326 15284.6381528305 14114.9434986661 30931.6471536481 32688.9165827722 53667.5875515474 144432.166701461 125458.980452046 2434078.50826317 165683.10107347 61563.9511700114 127115.165750413 26179.0180240622 46234.3451254302 20527.3879031816 17757.6662425345 74168.0436516458 27120.415942331 18208.1954410216 20955.1237780057 7770.54470439227 12391.2985038531 8824.70353873519 7871.48931273051 10039.0532284781 3490.31359358962 1563.06489570033 414.949906442912 0 50.0813010311303 64340 314896 9 41807.8541121997 8004.00388964082 15007.1924697013 12994.3043842515 29643.7573935052 33409.0709333037 56154.1472349175 130943.594417678 153684.643361219 2480418.61482963 161209.24722206 61308.9815586225 142262.143872388 25660.403441335 47687.1740436954 20796.0814344551 20696.9411054461 68586.8720390034 26093.4429613262 15042.5881025796 19588.6197473531 7306.11890640396 11109.3109817875 10057.5885336859 8733.12813493688 7613.71034384856 4191.15763138618 1963.08808444528 291.234690038698 0 48.6657275064579 64299 319535 10 44741.4670309632 8433.76894687421 14154.4735629516 12018.5498530961 34400.8894700568 32939.4513555264 55326.3550614422 116512.342262145 105986.526286026 2421862.49145208 145974.806624835 65260.4345012328 123484.682180405 27150.5844852682 45037.4992535003 18302.0283052924 20728.9798367129 73122.4645575507 28221.14985375 16988.8404258556 19231.8953727994 8277.15847305101 12773.9358771483 9915.07031631603 9241.69641396634 10681.617225472 3818.57480703962 1398.11242261014 454.186666605421 0 57.8577043666081 64583 315015 11 39034.4850415861 9118.73366415643 14272.3072481302 12044.5391151849 32032.9629704063 33646.8839529545 56009.643736376 123662.457063926 127030.689245387 2528011.72246997 141939.838918283 53966.0778899883 120502.272900566 24364.1880429975 45173.7349980875 18843.1560356899 21286.6033897508 78164.2231312931 25547.9205643695 16376.6045951127 20839.9008486869 7682.61570326904 13130.9888485725 10889.2450340803 9326.86528726747 8426.45551054874 4560.17463611093 1849.58351754726 350.175600267845 0 57.8204207144407 64460 316900 12 43624.7659769219 8040.76004815655 14083.6642742325 12322.890908045 31478.9070846619 36554.8822316002 55799.7115012018 129196.866152608 133233.808997718 2629922.00512958 146775.658276118 55130.1536099116 122008.972334748 24242.3969780266 44131.3432050055 20351.4541346908 17000.3353790354 67675.0520777422 26548.0872467595 16701.3472276845 19131.2946878051 8372.66969881448 11703.0803644192 9502.57208215636 7571.51769972641 9325.74532987352 4671.04705552844 1511.50788770533 292.995468420891 0 58.1438253811575 64142 323426 13 44090.8315981845 9015.88072118686 14247.4015844985 12337.4276348761 32182.9101236379 28497.6123974523 58094.5525410706 133484.224817144 143926.292949665 2450048.73916193 160351.313460585 57169.9357649762 119873.657625561 26709.3188492096 41375.0687563239 20622.7564298956 21251.0085242092 63355.5647124268 24635.653206021 16887.6683372431 21705.6492657369 8493.69921281632 11918.7374659543 9473.07112934125 9120.96414160532 8490.18513823766 3881.32393340444 1618.14422380867 285.576996315404 0 50.9216296488559 64207 317394 14 40626.2008482979 8592.25923578985 15737.7189836052 13211.9041720986 32401.6765491527 30337.2372521622 52588.3714985663 134960.911192388 124093.502560781 2449309.59885454 159554.623278108 63180.1819652035 115037.182275581 26817.874344815 44810.2997187304 22553.0892127682 21271.03795058 73029.9178831795 25267.6217009219 18474.2776787705 19392.6878726706 7740.37709060368 13107.4168753137 9527.53060215635 7761.99789502841 10163.2751907881 3460.45506078898 1632.10877723678 295.510017704392 0 53.4603137969056 64517 321675 15 42988.2485910698 8044.91644841519 14453.991241793 12652.6760448405 32589.0810104168 32300.9406361638 57022.5686820578 123563.977949434 114592.918948875 2585500.09953347 153698.508769837 52651.2223101241 125113.489680841 28599.6892500368 48544.0802720724 19593.0408168459 23641.7434789632 79158.7873876152 24298.5590951442 17212.2398926953 19727.4615230986 7712.26920767425 12590.7607105468 8716.17614796043 7748.94962810358 8377.08992303801 3832.17181079661 2004.55469654961 356.025354420001 0 58.5089760116739 63732 309955 16 48333.4456975537 9351.99357090535 14104.127656257 12954.5282622444 29185.3769756731 30708.0360487582 55009.1370981311 134039.733948583 111254.527123777 2512507.10396986 160174.614649709 60806.5722183048 112784.087949428 24969.7104277938 47177.1778968201 20227.3771656459 21607.4742819824 70733.0104901336 23272.1130432568 15484.1538373206 19852.1954074361 6923.58798658324 11938.5676909824 9650.85761929919 10024.1701960928 8801.39067587668 5744.49343254656 1615.23902572288 340.756341010901 0 55.9846675756473 64190 317045 17 39495.4966369679 8336.54927123818 14003.6381105016 14008.6584469923 32470.6893106408 29707.9101803201 55491.7211917996 129227.360740774 130566.659206856 2504325.27548477 168370.252040979 53722.721196151 124069.175481753 25648.6795936787 46873.0954897357 19195.3890617479 19407.2137385606 72611.7485915263 26872.6733819837 16207.8927709633 20812.2363731374 6912.16894241093 11995.8411357678 9342.05552432682 8452.64084374348 12288.8012445089 4920.10919296263 1605.24080274798 348.449377486105 0 52.218643465364 64514 319117 18 43374.0138471013 8510.70467586302 12732.6820406106 14097.4919746083 30158.6754393685 29807.2358966095 53147.3054487677 124809.116048509 139082.284025093 2530132.99793942 163419.333452631 59189.4577802736 119900.843367388 24775.3471039142 45847.2468666036 18440.2697203435 18856.1451609226 67620.155688237 25574.7625263392 15725.1422944316 19502.1955763803 8667.24239203715 11169.1619770738 10087.2182625262 9434.28744047521 9986.21626920084 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13255.1609285207 31950.4551861417 36692.9123493953 53144.817054646 143208.471623983 142929.946164285 2410185.06618708 164830.452123299 56606.815444996 120658.614711607 25763.926320663 46082.7267508996 20136.2883470546 16491.8919036331 74367.3439346363 25067.6636715653 17171.1057854034 18019.5423679019 8673.35779166992 12042.1188452558 11221.7009239305 9542.21294188135 11445.3742309776 4309.77624749645 1717.24648052914 411.516641219229 0 48.4651315313682 64600 318324 90 48880.5575998876 8511.44470383682 16557.9342849798 13150.3465899518 34163.6143198202 33179.6540678528 56802.1888258514 137131.770215202 151849.906302121 2458086.58393617 178000.798049797 65285.8478588746 125103.037218095 28101.7070959544 45107.8723778704 19453.7829804688 18733.3515330524 68497.39667381 23616.6288042276 17721.7779622074 22539.8866896881 7969.03715725555 12002.227415459 9928.03322427532 9291.01588876878 9304.89437198697 3900.61949457529 1517.28623803457 485.643389310057 0 47.5329348733338 64467 318654 91 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10898.2143141748 3550.16522026018 1517.30221550397 499.681108466003 0 47.9560871331151 64895 324025 95 47772.1381820862 7592.65720388757 13852.6147658106 12589.8859915289 29650.0526829124 29423.5255950847 51221.7624236224 132857.257437 126422.783415596 2596030.90962769 167708.838682244 61163.0257557022 124545.00358979 25720.5942310144 45849.149675424 21889.1879696194 20001.2861062575 76217.043718285 24391.7072696466 17822.6259267238 18825.2037249158 8058.01053776645 11840.3148435966 9808.50607324594 8022.03692178595 10058.0140254435 5275.24831989225 1495.88056014934 303.131822343146 0 54.1383090447358 64715 322548 96 42941.1300566159 9043.30561289338 12640.3579778974 10574.2937433381 33360.9494705696 28688.9136699802 53043.7702777944 139520.592406611 131153.230872039 2525221.6572897 139759.12301151 60438.2939561415 123551.925140871 24223.3844601753 45816.6908001893 21592.3526030644 20791.9619179305 67035.0299354517 24350.5707061363 17116.0481512573 18508.4721514923 7242.81077955449 13005.3511681964 9123.75653474904 8137.74245947347 9597.89982016519 4602.07760242544 1522.70425401441 408.654491229285 0 55.4270757444458 64452 318895 97 44159.1933047281 8514.4894132367 15319.1455175718 13017.8610376161 32338.7865644348 31118.6779595832 55454.9459916391 121165.79316077 150826.42482088 2465904.83880813 164331.040059627 63833.21071386 129499.518271284 25928.2550787943 49142.4459991435 21686.7103024562 21024.2529984872 72042.9570314802 25724.7275540329 18532.285222447 19691.0759290915 8947.61461029416 12931.860168608 9396.95057195599 8081.32535821937 11441.4313018875 5519.50546159704 1584.51818222925 354.668479914827 0 50.3980115115027 65211 324448 98 45527.7097087323 9487.50892223396 12686.4090685017 13630.4580541701 32947.524928504 31019.5397838468 54555.0283953299 167966.206331618 131803.881582687 2516224.09939717 161994.990481618 58806.5689104498 121251.766230205 23533.4504805497 43253.1874819942 21111.5544000855 20995.2836070399 63875.7462483099 23577.8550477849 16753.9473795532 20054.6720549343 7925.69151114807 10351.2301201529 9684.72780492327 8732.52294777689 9063.86250275424 4806.49217782314 1532.66703739977 238.105297564717 0 49.3738916874224 64293 315573 99 48302.9281405485 9976.71503234515 15928.3897030796 14643.7656123216 33553.6595699087 35716.1374883356 51505.8378684486 138645.173646741 129776.096565627 2545490.73768012 167395.628443912 56472.8836536774 117468.000469404 21951.9166343444 42880.3254465302 20381.6558741809 20945.9807093045 65751.9011374537 23536.3742548196 17118.3801437979 21320.2147429054 8220.48657037533 12070.149598726 11009.8005338609 7129.82329978479 10688.6599053031 4308.72979202126 1785.31928313032 404.360243138965 0 53.4163549488645 64853 323612 100 42578.616181168 7989.79087693059 13099.4425128381 12782.4356539426 32728.177387351 36369.8962283736 54473.441475612 131751.007980694 138649.304540907 2404400.69084676 161192.30696334 57435.6259090139 137765.103351752 24816.7991270208 48041.2203413906 18969.6884078258 20738.9807945966 75049.4246577404 26842.0678782494 16694.4939796386 21624.8908320103 8217.55184642619 13421.3688115583 10910.1466111365 8651.30795261738 11743.9053552775 3936.83333308135 1661.62106041344 310.81366494449 0 48.8897064001458 64350 324659 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 45274.6403641471 8717.55293198017 14192.9576600736 12591.0338419893 32830.5871251901 33418.4365344502 54167.6105345372 133959.614407736 133418.988375637 2489305.44665048 161156.265735015 58965.1135811589 123991.497531854 25007.9132780744 46427.4330148607 20194.4652578002 20253.5136280383 71212.6647333819 24819.4229931977 17133.2069232117 20294.9700479866 7838.78839130989 12166.4202260563 9748.6592986076 8628.71037137162 9637.77092030815 4313.5745527431 1667.56476058671 353.617884299572 0 52.2130943010635 64361.34 319154.68 DEV 4934.20617719818 761.54911433089 1235.58951293981 934.759188164381 1625.63928743389 3544.93860638131 2330.93801295188 7993.48314782824 12958.6997355376 87232.8326483773 9294.04629410275 3517.77313211631 6022.16078882566 1514.30911068324 2504.75288187567 1185.57550756635 1367.27854334037 4362.75569830888 1540.28449561659 1059.32704433376 1155.4906119318 568.696786613314 997.5883919646 639.096615062766 736.607177713208 1043.1995720441 622.538833734649 188.544069047189 73.625194950995 0 2.75905188275164 389.940180540554 4201.24504136571 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-150_preproc.map (8888658 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 11194.113915272 17094.5103365212 10781.7663773873 9242.25823708241 19327.7568564076 21147.9015698254 36335.7173535969 36837.9392844421 109612.662532481 1348983.94793377 105950.023136626 49811.2650451513 88779.9753874682 74411.396185852 3971315.78835939 19105.323679905 19556.0960045643 15173.9410097297 12380.5839443952 13841.370093255 9356.50171055265 8250.02681033888 7286.97604506685 7035.6042617924 3457.01333506233 4860.40766287478 3087.02424472493 746.324683923158 466.505843860218 0 1.25666666884861 45581 252532 2 12019.9892994577 15270.5602282462 10474.2550968007 8989.18131688571 14358.3199102267 18301.7605017796 39492.5690044749 36320.9933437618 121991.081813177 1228634.64599947 93461.8404469185 47512.1227626826 83995.7736700172 43372.1459817408 4232074.19009877 20609.612211289 26654.8499807716 16675.5777701119 13461.0978336996 12855.7443136078 7705.93342630723 7987.65450572962 7681.01273568073 5795.2740148223 5263.13579726299 5063.55300981967 2585.73719865033 1052.62933228266 421.029704797614 0 1.03711518965315 46077 256895 3 11729.954895901 16131.1133569719 10784.1673635435 9984.58351211322 22211.789449995 19317.1584521376 39330.1169857678 38817.9318329512 82288.2724706563 1256361.76484403 91627.3087124159 57672.2951565585 102246.635056795 51023.3366098212 4137791.01886628 18401.3666431068 18535.4382039843 18226.065274234 13649.0057554723 13815.1509401236 7830.92868822062 8938.28168431678 7841.06326082034 6683.41994212532 4198.97531005076 5672.14660857188 3394.1214809355 1020.49393617525 440.778614307054 0 1.09669465416925 46344 259385 4 10552.4002253652 14357.0592571624 9026.77871953822 9468.84155535718 18477.6747707996 18486.285586809 38394.0483485228 40760.6717776521 85152.1307245232 1302307.37735556 102282.079902115 43658.2491763313 89566.2426675104 49276.575529095 3870377.09228325 16738.53144592 21063.9301599775 21263.1558377226 12001.9001819595 13273.9854972441 9170.6204854234 7690.1667489338 7696.07005002427 5876.38380352707 3356.56724014044 6334.65918487 2075.33155508376 892.890889089162 424.28932988013 0 1.24409459271614 46016 256611 5 11371.9167431386 17715.4843387199 9534.65041844773 9246.3923744922 16478.9780006661 16396.2064729534 39837.5268811122 39604.1208986084 82769.1952531968 1258434.03031671 101384.292739896 53535.6677185872 88590.6449862875 57410.9082893209 4033027.86017065 17548.5611622734 19278.8977615614 15194.8128872267 12061.325732759 12448.2723472356 8707.79656501342 8557.27191141381 9733.11235212094 6754.34437529912 4660.73932777186 4066.54669743092 2052.86542576098 833.197158768207 273.99316066559 0 1.1345546789614 45683 257471 6 10768.1895412556 19634.9392328792 9676.99348564355 10090.2671149019 16399.2216647015 20715.4384296519 38829.8057364229 36380.169409482 84021.2372208787 1243093.07599364 98713.2164650425 49404.9146228376 87226.4373110805 60209.8549781471 4446311.75304947 15537.0547826843 20074.5957166277 15554.347164686 13477.0496380445 14004.983929152 9130.25542502955 8434.38668351547 8973.78454614292 6875.12366022174 3687.96441015185 4755.06810233996 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6958.41776595969 3665.46861306292 4852.5221520803 3134.93315275613 886.924697492844 303.178392238441 0 1.04374611134828 45319 251848 83 13642.0838242637 16160.4212955693 9523.97424899164 8310.82760216105 19077.5642957402 18402.733115526 35418.8635085428 34682.4690073014 87904.1105500571 1258472.15527354 92385.2695184713 53086.972839267 82088.3685536549 50668.2637243624 3834155.13761556 16361.1788587079 19188.0572090759 25560.6170521366 26767.3490026008 12597.9348224848 7876.36828307403 7475.82343694623 7628.54881357681 5414.97197670559 3237.93580951382 5258.37389972049 2394.10714810512 768.385210134601 338.322314704627 0 1.20629104986331 45635 260891 84 13841.7577222907 15198.1270935121 10094.2948059735 10211.6091802159 16273.6481008611 19746.4394051272 41425.5526086063 35909.8207577923 65899.3266669595 1257878.59523427 101214.837137509 49405.6345319718 86181.6193435309 67102.6630679928 4129892.01993241 15256.1043658764 21882.1340218683 15078.1510147785 21044.9423057122 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27018.6025106531 23682.5264220073 33406.8487324185 11687.1506758331 9798.15719299436 8301.6199098346 7977.50370959425 7352.62053211804 3711.07231409307 5514.21701100682 2631.95617618784 844.709801317175 517.512929658959 0 1.13859408994412 45536 254949 87 14659.7922008226 15379.7275093475 10551.7170077903 9267.519579782 19319.9859515967 17204.5416498187 42304.0646675994 38266.644804573 95670.630482374 1177613.54030733 90402.5383487093 50413.3505648001 88372.8181138281 46028.2527135422 4198665.83155717 15529.4201222528 19132.9994609553 15642.7712776794 20999.7634105373 14682.2319038555 8317.07578990857 7405.30184630114 6752.83371327655 6847.88688390219 3427.88442890528 4616.71277644062 1634.99247721276 931.927726190956 401.847034754384 0 0.993103214398617 45335 255867 88 14669.9570033819 15861.3868424401 9816.90592177924 10389.6359538254 16075.5909013403 16926.0057451475 41041.2813166633 36058.8736056929 71315.2569353294 1206529.83572554 98151.5745507423 42904.6164939393 91822.6132177903 41125.5161836412 4094834.93245026 18334.5408976531 20855.6533268261 23129.0621677926 14796.3796917423 14135.3560613331 9297.43205183539 8334.75468734623 8013.75123150784 7680.79316829928 4360.53941241058 5938.68390402116 3748.64203997187 899.756847330133 628.312959567006 0 1.05758259332788 45857 255003 89 12753.110506954 13861.3989430074 9417.76977914543 9412.91962255446 14996.3345304723 18030.8273863085 36177.2608577137 34129.9367164825 86337.8614992767 1160163.4731619 93810.533977975 64010.9015107119 99795.1458482684 46022.8425129989 4237716.0072331 15506.715521968 19922.4755519482 20506.1021820666 13064.1784960145 13099.4320002499 9384.11286316204 7095.47669952314 10138.1634890887 5469.01543570652 3171.50746512518 6094.97214641229 2556.94589038271 999.578556485238 473.78996130606 0 0.963189799520853 45534 253410 90 13196.3813753701 17764.6117020608 9310.06771216425 9130.23425477656 18338.6340241504 19993.2174780833 39577.4640595908 37497.7736732869 97219.4345956502 1285007.7922412 105014.65459123 49008.0874415569 92019.1494279527 62843.9667737285 4242421.21868311 30444.9997116793 21296.5485943255 14947.4121886567 12888.0054085508 11847.3293223591 7529.60892174615 7718.61454247962 9562.94430718436 6530.1773832417 3896.49404875725 5582.8678696707 2901.44609859204 1119.47160331943 367.275894735577 0 1.09288964849461 45568 253088 91 10202.359412288 14395.9354814378 9992.52970253167 8861.25368934816 15756.2362741539 17637.0357616814 40324.3496770013 39910.9473346531 96097.6630444964 1243061.71767833 96213.0244431183 49670.0456561979 86750.1242411609 55665.6117208043 4118332.10530684 21304.6021661005 19640.9570733266 15966.91242016 19674.8920921614 13565.6413610262 9659.64846194739 7475.58040598935 7687.46182032979 5845.9202925762 3698.93869745637 4636.80162578619 2533.20549714085 1269.00555730884 376.270021895419 0 1.08862531730941 45038 265784 92 11134.6070048049 17409.7133745826 9954.98916334271 9098.51878033269 15937.1496557426 19795.6847351548 40130.2062209147 38466.796266977 76748.9257176977 1255034.8802227 109893.813480264 41219.3993395456 87246.3241271498 56143.6764750431 3932015.49670514 20739.8604845095 20225.9909844172 14001.2808240344 20528.373223885 12935.5100892851 8247.2378323609 7256.81990893624 6889.52836323408 5365.70347059136 6124.84063274128 5484.70570395755 3480.2955860436 798.769379816718 432.669102920476 0 1.16609128345383 45284 253859 93 12635.0614831782 13558.2181995055 9585.44711140395 9013.27798761465 18224.0437120059 16531.1519400393 37070.9639794469 36857.6355814313 99536.8700376796 1194920.39714494 94863.6992323297 50102.3657169815 85458.1079394565 55712.2405001603 3983986.34478276 21942.9734787846 21465.713853531 18175.9724328833 20659.8999329014 11588.2003005308 8618.53281226243 9532.92292273622 7291.5686301557 6882.01738781676 5219.07536946147 4995.79806447299 2032.0964507952 858.534118809095 343.709802763387 0 1.07901438535653 45715 257789 94 14143.097265526 13929.4687534754 10224.2505666604 8913.060157217 16844.3650130235 17031.8632324835 41338.0442723376 38370.3353839863 71362.0796162871 1187853.49400207 95983.8484603558 56511.1901918722 85098.5364525836 57017.238063154 4099440.42638151 14157.8555414576 18343.507928446 22984.0114297678 20575.3334969439 11222.7436738191 9194.17384439788 7533.92247977112 6321.62230294536 7381.16221290838 3944.2757082547 5206.41519569863 2465.66123943967 909.750702876699 476.871550601181 0 1.03482212527733 45592 252999 95 14144.458486358 15472.5659302039 8439.14685786996 9430.99773708312 18018.4170745424 18326.2628637951 36548.752082077 35204.2020473499 97343.1460216361 1287558.04890826 109619.238006982 62518.8992829001 84558.995311446 63768.3447189991 4182255.99307646 17040.6790353483 18571.8005801042 21393.2293562852 20237.3448183096 12151.4187004341 8281.3767980951 7787.9849793374 6899.35930186563 6676.04723575398 4344.78058398974 4999.07549896005 2129.64106132094 1106.65818340578 271.287912205799 0 1.11487165250392 45848 255476 96 13380.0757297167 13104.9395381166 10519.7352468675 8796.96813100132 14253.6623891903 16201.6511805869 38340.3795550967 37000.3715500304 102174.642007972 1301480.82514943 106811.868288793 54788.4580119746 89194.1632104759 60917.87241635 4278257.50240008 14588.6610261032 21698.9697962539 15454.556315663 13742.6642284916 13745.5621020645 9834.14175306935 6582.70440532507 7124.32476872604 6587.73713101628 4196.65137654443 5378.15397892827 2900.16858218675 1043.95378209232 345.282408511681 0 1.09974023887148 45522 252385 97 7575.69621414006 15245.7879103635 10122.7120720234 9251.39066804411 17001.7982948391 18693.9968278616 40752.6825552001 34997.1412914818 77529.5330928975 1218198.56230993 97032.4823252771 54376.074714991 87408.0442873979 52633.4384098973 3717038.13065877 12025.024760454 19752.2807629456 18424.6462070343 19470.8408659692 12057.8994568953 9370.34775553214 7678.96595946657 7503.5239676977 6954.65553436257 4611.88932752473 4886.59517178141 2952.83238939966 1036.23048658121 382.748689479563 0 1.20390838364535 45609 255086 98 10658.000995165 15091.7437491248 9661.66120325938 8349.51919068873 16498.5710455056 19982.3284667985 35977.8288347137 38039.1096995698 87364.7329561068 1311915.78119094 103142.789713117 46236.3969213027 95653.3791434988 56359.463381616 4190319.95822776 16034.4015582744 19905.8911975462 16273.2852184199 12487.7674314652 14570.8265239363 9498.73626885713 8066.98614862742 7281.21083961245 6912.49345840747 4496.28031524281 4664.07228938986 3683.75396810403 1092.61252865357 393.069798638414 0 1.14022503830353 45802 254063 99 9988.7900097147 17589.6340782005 9431.32316324219 8399.78429425054 17554.7238152104 17811.270010117 34901.3155137239 36549.8578607651 101033.331519147 1245412.97106249 102332.486034073 45012.598473574 84744.3084687674 44724.2266648985 3960680.32362671 15004.3990746415 20033.3354547848 22299.1516110628 19613.9494934304 13112.601021467 9885.22241812985 9478.84161616473 7683.47267418673 6096.87417354826 5826.1011057106 5274.49103072525 2147.77449784274 1059.80566008193 399.246165806277 0 1.144921486845 45393 251070 100 10213.5016546577 12682.4462737037 9818.22961369332 8739.15669157706 17024.9852914928 16565.800798063 35753.54349164 36576.5923233954 87053.8465449795 1226843.88135599 96990.8263498285 40515.0613684012 88345.3006590764 60021.9863905512 4042151.34549502 19789.6054328233 22196.2841808459 23382.6414566075 11799.3571504489 13821.5175422512 8747.25599795668 7460.70136965617 9101.69890589123 6625.5186629151 5669.98628462667 5525.90150434102 2584.43822565235 820.881372969905 328.380936000862 0 1.09657589641531 45992 258191 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 11948.1961191539 15693.0525084693 9854.78054741449 9131.20512515025 17157.0513913049 18227.2059823137 37848.7951252111 36976.1202585842 87894.2340956687 1243204.51824929 98862.4634120836 49958.8100076644 88399.6181028596 55704.2346426204 4115933.9111141 18076.3980420578 21329.4519811555 18644.9311181404 16634.581264225 12890.3146242191 9002.77232165439 7897.92553739806 7996.68033210351 6637.80857329414 4273.84252403183 5208.02660332943 2715.20037521068 964.324161089839 369.838364419566 0 1.09201336166553 45569.06 256215.86 DEV 1881.18044571397 1795.52659145487 570.808049125886 705.259625961858 1717.35516819616 1457.64365363784 2429.37052587138 1741.17880312586 14816.227669725 42691.2296370686 6692.6491509343 6452.85648559446 4350.83213120888 8778.70835665263 162195.464072287 3965.93750748197 2684.77766860191 4130.42775682557 4986.10504963474 1035.53991729135 747.937421451055 636.54402223674 981.307618656109 651.694391640215 687.921471281081 618.151103209245 514.18270444763 165.239118129281 72.1012993566498 0 0.0718831447260135 321.686207972925 4506.3658351714 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-173_preproc.map (7987330 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 36772.5277617648 19316.2339537702 10642.7520269067 28105.7201485728 22005.2817694551 30509.4783163383 35099.293890026 66492.0504959769 163123.763308846 2006466.67552895 135212.433198388 69104.6383726839 50012.2134545901 35246.9826043148 15677.2315637061 16810.9997460914 18430.3865410145 14717.3915726211 18760.4489427973 12317.8593089604 22390.8320625996 8328.93037576429 6707.61507661513 5940.92000928095 5463.4679451751 5377.88710918128 2742.62289249361 772.365263022309 286.512421866825 0 48.51649733468 55190 309684 2 37313.5535183784 17163.8061870022 13082.5340023917 26294.8382707308 21066.7196122805 31439.8661140435 39743.5544090079 67990.8711489013 165329.669213775 2022161.21204935 138679.626842911 71293.1296389873 45584.6914490961 34929.9280285727 16177.9499963462 19042.1322137507 17485.4416904496 15437.7202012743 19122.1381259033 11509.6726312794 21985.7278837159 6837.12022003723 5792.3648792318 6601.9773259609 5263.31377311443 4229.48041763255 2918.46848803724 1096.71171470038 681.900736350447 0 47.9548297204971 54838 315584 3 36746.8033667062 18072.4445308943 10716.3408715187 27823.967933277 19372.5161958869 30826.0468768381 40891.2580452066 62421.84282396 161135.712987022 1928719.24409613 129119.636926537 69744.249219713 47665.1720993839 30733.2417891006 15579.4930430517 18715.6804020657 16865.1162824888 17281.275823262 19663.004844068 11375.6627534391 22097.3070638569 7816.13572614027 5868.38310853831 5698.85768865236 5680.40285094748 3654.40641533622 2289.79918701819 849.929434489146 550.038786272883 0 47.9344493575873 54961 315008 4 39823.7393599447 16491.1705973258 11517.6617976196 25125.8098089945 21275.111537456 31103.4007362432 37548.9045028059 68518.2190085175 178412.584647967 1971602.44935691 136414.273156173 67495.2424999808 47403.3875199151 32832.9192751683 16164.2447814781 17674.1146905191 15348.2076670161 16000.0635849211 18648.8034169416 13517.5815392478 21446.9049678248 8679.53802658069 6253.50862658105 6564.27508054909 5721.21135219388 4046.52862213731 2809.04167850942 1367.17177752052 1240.67925039093 0 44.6753883216455 54934 312894 5 36569.4213583703 17859.7241361791 9697.01141232762 24362.8580940484 22846.9649992996 30985.673359054 36392.5180873618 55415.7245796391 158954.44441525 1936603.63566419 133145.513009615 71388.9866178802 46383.858915579 31150.8535591056 16423.536453955 17744.9263178023 16731.2040846131 15583.4056795755 18167.405427538 11680.4880700866 20972.7208290631 7712.79587626904 7211.9814906384 6267.2732953704 5359.33652989535 4523.61834490285 2542.18504144744 1165.42872299917 471.942696194859 0 47.9699063256004 55301 322168 6 40398.5041298401 19209.740138365 10153.1998979685 27156.391640965 21464.4009992427 32033.1188885156 40099.7840627363 61892.9179180383 169800.876596358 2006709.67037508 135164.696362592 68927.5970158917 48756.4103665067 34574.941938341 16025.6994692898 20165.3594423348 15285.6061193197 16480.8435570928 19809.5768120317 11540.8095561827 24973.5662658819 7010.1938657531 6381.90313323841 5899.19411504341 5132.22047867228 3854.58084449214 2927.05490013317 1385.55600525456 495.097711969721 0 47.5301774589175 55138 318760 7 38652.9289437116 17252.1082592395 11563.5577545826 23712.8002825478 21403.2698184655 33569.0827554244 37278.4112977596 67549.5050301351 161903.326086203 1970503.23203977 136474.348043817 76619.8863997343 44004.9468481811 32690.5368612588 16279.0552507896 16663.2786721963 18792.1778625649 15863.4363884127 20840.7641927404 12926.2119735464 24760.615947869 7743.36581067718 5735.46937809286 6039.03107430427 5462.23851502702 5221.85983081898 4205.69391492576 1064.27059227701 849.357724738439 0 47.3836345215714 54621 322575 8 39528.6976514675 21737.5930240777 11617.8732067229 33941.7527354402 22322.0161336644 36746.446695204 38154.7670388011 59220.5911927046 175215.370530518 1891612.52137827 132098.800940395 70211.8028699994 48174.7100878096 32329.2843049702 16306.5154447551 18337.7064596746 16469.2236470524 16280.9961595506 19260.2085620076 13080.8307403944 20076.7570636555 6549.92501146869 5697.07778583213 6398.40653838297 5501.36313275015 4412.82055674433 2196.99425312428 1201.94423128673 488.238685555962 0 44.4999285695406 54878 313177 9 37027.0301883879 16653.4986342431 10261.1995681885 30167.6047738947 20646.6364386775 31526.9199281625 36377.5701768217 70152.6320897429 176897.594373611 1894567.19877375 133223.010269216 73255.7038041329 45046.0812703299 32987.5729371678 17456.2683460832 18977.8494919158 15594.2920438055 15151.6161908671 19276.6734536309 12969.4769018209 23980.4553105355 7841.34362186692 5559.84927292063 6486.29930707501 5758.96077471167 4438.21921954231 2360.71172782324 1414.6063294865 611.352530050149 0 43.3382088707169 54697 311214 10 37880.5342214117 17134.315997275 14456.6851447609 28462.743172248 22312.1918728038 30129.3623424795 39109.4009098564 61922.3250018227 162014.097738646 1959082.26318246 131755.484886824 70848.0126817259 46980.6448459623 33140.7870073239 14960.8308394418 19489.0609365324 15945.5573331795 14048.4804641103 18733.9204645165 13748.8402377813 21222.990574441 7619.94396639936 5868.46179325776 6485.92994393612 5832.63419358464 4499.17932849837 2482.90601603432 1397.87983006512 482.816759903461 0 48.2987390529519 55115 312933 11 37184.9226295097 19001.8139533627 12088.6863124765 27579.8909352786 22921.2532693674 30162.5533965855 36070.8925929717 50525.5850861782 148880.155718313 2002510.02485967 141769.584106907 73957.9265432432 49289.0367879255 32703.7660249873 14853.0539724328 17244.958070998 15776.850851555 16281.7022543507 19023.6724677568 10922.4268643301 20763.8543087401 8302.58843776628 6537.10194701417 7613.58018929121 6696.69122706111 4755.69027803656 2925.16590150851 1552.83918469308 687.19493754149 0 50.2349747710241 55191 326210 12 36902.7215639626 17214.2635239759 10543.8392136358 26769.7401635501 19860.7156106532 31835.0967813223 42843.0547892087 54336.2996277088 156112.894456304 1934985.55227891 138993.23366095 74789.0545350576 44717.7615762374 31591.725884872 16829.6391432652 18161.7534294691 16707.1216960616 14512.6163868348 18500.9950094376 12464.0280295221 20818.6618438276 8155.06207956251 5466.50892741243 6571.22195003949 5222.95382418462 4516.97059462724 2314.00814184832 1608.30003119943 558.701011994132 0 47.4691009132757 54960 326471 13 33460.337172979 16240.9246803235 10895.9043081397 26499.4842834067 21777.4686301172 30766.0488033524 38017.02782646 56185.3210751541 158026.03596364 1985100.71442332 135933.387949294 71331.1066099293 46197.3216083913 33948.4709090477 14787.8053773841 19540.0208920531 20779.8178619232 14658.1421805503 18881.1614385095 13295.6053866705 23241.9533219039 7582.74758997236 5807.80603453901 6201.09068932338 5578.82650704552 5498.15026373079 2379.83941066413 1478.32331555093 455.694175697666 0 49.1597627286604 55209 310280 14 38358.248346672 17953.3673533821 11522.5306112301 30572.5535379917 20705.3963639235 32553.5959108443 38979.2703785381 57729.3638841023 167249.426507859 1942218.38029767 137574.645384329 77079.4278061064 46889.2183972927 35110.2036442734 15930.1885927732 18994.7306967188 15676.5663963641 15171.7849527902 18424.0062859637 12097.3776271504 22229.0485050954 7845.28597909419 7411.8933082031 5866.26350330816 5345.93070064211 4744.43121393179 2594.41788197486 1332.0407658472 471.588258979128 0 45.8331613086915 54486 316556 15 36165.4317789561 15583.770041643 10012.1254791895 26864.1952495802 20836.5949723643 31818.4095110084 36839.3963058436 65824.9284699599 166771.624661809 2002463.07829904 130213.457243497 68490.2182785986 44702.5005716632 35021.8393965315 16039.8191439241 16936.1643044526 16358.8004530367 16864.4665902302 17479.1056575688 11281.6378429692 22676.0279735128 8062.07035726342 6239.3295696376 6165.32483956263 5758.07202599091 4631.36300640937 2774.29062480155 1390.13549641286 859.730533866442 0 48.2709427058046 54309 318775 16 36526.5842861643 18349.6444854799 12286.3047748519 23314.0782249667 21090.7020997359 30325.2794379352 38006.3454909665 61595.2201160814 164132.88692324 1980025.07490169 131427.194144815 73924.0400445939 44707.296771093 31713.3178310416 16529.9594608977 19465.0667763046 17163.2661084741 15243.9507239848 18380.8675699123 11827.3924967166 19871.2974841601 8159.19894438181 5826.27214266603 6986.30489101107 5229.54829507073 5203.95213040631 2291.67044796835 1181.63546871335 1278.10659902784 0 48.1579158458073 54965 321362 17 39767.8354827297 19021.0426813567 10922.2217939863 28651.4968165377 22493.9460241842 29690.8248901739 39714.0377382475 59713.7996397614 154982.039027686 1971402.31597152 135210.411849915 74581.4683835467 46037.3322490145 31985.0551423386 17024.6043839237 18196.5923105841 15620.7487243023 15949.0436638864 18130.3206173757 13675.7046286481 24116.5889728374 8486.20361949489 6411.63715525658 5710.58972440084 5383.05470331843 4819.65016026062 2459.98612685026 1036.33122597274 425.997655843334 0 49.3147824272965 54575 311225 18 41170.9524650953 18306.2335843418 11106.6901349633 28467.3995518799 20688.1797049133 29183.7886923362 37468.4004259455 62680.3237751687 164273.321389291 1963917.4404264 137381.033065333 75739.9898950869 48826.4385999528 32798.6098362504 16996.2533859956 17191.7545285461 16724.2012845289 15225.4502306478 17414.051881216 12191.7941903998 22624.1148109168 8390.62008248766 6197.00954456853 6578.91791550745 5336.89333218567 4382.55296961404 2524.31210475047 1896.14192892549 529.79388257707 0 46.6986527695505 55072 308068 19 38265.8918841826 18770.7871447865 10642.1451526153 28090.7734032301 23400.9001306928 34470.5081993437 39876.7156460164 60746.5185007221 158217.284087548 1976216.09559116 133046.198340894 72936.8961834076 47862.5418876391 32491.7728852598 17187.1496008342 16462.7297022755 15413.2132050721 19746.9795094875 18309.2730641378 12872.4266494554 21705.0271692611 7939.56487654357 5385.03474982187 6759.01982829131 5719.93207350165 4151.31554235277 2491.31316525421 1172.53239504465 1072.17570164656 0 49.2989872547447 55248 312856 20 36690.6128870756 17389.7781691754 12676.1719043327 24796.1423952856 23295.3509292833 32512.0194827097 39125.9339460402 58086.7963878758 163119.570275139 2009549.48181062 131911.207652773 69359.252708418 45220.4624293937 33431.1966489724 17708.5020274526 20330.2966757821 15334.6457622041 15461.0660818398 19004.2351599982 14002.5554733414 20923.3662806468 7464.57664574523 6174.65248009045 5658.54848123396 5962.70072741683 5246.30687074787 2712.17918822582 1291.04926985916 793.514342405563 0 49.6372335800347 55289 316874 21 40137.9881631749 22925.4761859315 11733.5936452805 24945.3534738525 21339.4630477867 33003.7284820039 39205.3228286109 66259.6352210544 174642.360178519 2041795.37887878 133876.003426491 76441.0366481787 44830.0467032386 32641.0131400626 17979.7575745607 19268.5556452309 16354.8834992999 15037.3903664884 17709.3629824537 10562.8377739597 22084.8375025982 7766.22651199054 4933.61233958579 6610.63849678489 5345.98293765842 4723.96350929877 2610.91605654603 1085.33033211087 650.493550762413 0 47.2279058334996 55604 326603 22 35481.7170937802 17349.6811434522 11569.5993045042 31164.1191918649 21721.3873999802 31474.7076677843 40388.1590177655 63254.5953184065 188811.084793593 2071800.48548547 138648.415067414 70067.6675296848 42662.2617415158 30329.1245366004 17598.7315596067 19334.1337599596 16477.2569145783 16286.0556366795 19119.9596981165 11521.2790151822 19128.5787082007 7844.15192666125 6383.03033842863 5839.51170496222 5946.06999420989 4386.88195949687 3235.83840983889 1052.91677389841 1100.58692886528 0 45.1626619928686 54885 313390 23 35681.6223701523 18733.770646802 11525.0313930535 29103.0547512893 21506.4115761166 34066.7265788156 37857.3158061651 65115.5247552596 189822.15895548 1980262.27355856 130820.913301232 76645.0248160098 50901.585582606 32276.4050667678 15472.302164694 20190.2530527091 17662.2366962708 15859.1987507938 18656.1948964426 10884.6389963818 20560.8019868012 6995.25292953445 6752.15026604296 6221.29324445564 6069.63697185692 4616.23645506174 3061.08921157497 1023.05392776691 259.452532499693 0 43.454697681154 55429 325676 24 37993.0913812584 19174.2632705889 11846.7912368107 28130.1991504777 24006.1735873674 31420.5375599004 38165.413200472 58441.3432325972 169938.580322986 2044466.93171139 140920.181941172 71547.4061367932 50403.5053364488 35675.7672537047 16620.0028096204 18767.5122668103 16342.7691315539 15677.6489892882 18037.4296796226 11698.9035109525 21457.1088295942 7532.74970429533 6447.14137304277 5894.66073430723 5968.65953036633 5643.37377984069 2718.47677302142 1001.82391133647 334.537549979126 0 47.6616469148687 55525 324852 25 37094.5808367905 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21925.249690294 7656.78613400328 5749.64412386284 6897.95138994681 5923.48106802594 4426.45512547095 2442.81119077742 1933.29707612002 559.45479774947 0 42.5747883981042 54897 321710 90 38190.9949734006 16505.8899923649 10784.8866850692 24233.5357545412 20039.5164634101 31538.6011729482 38440.8896898765 66846.4250403607 159937.019235478 1914558.72183038 132060.451963278 74192.0607666161 47207.7237178234 33890.3344373291 17281.847317748 19241.263268124 16167.0149433112 15198.4124349061 18067.3830062824 11068.4881901515 23786.870072433 10146.2697990345 6221.60290000426 5731.47662419519 5465.68212430701 4446.47854174418 2485.89972162593 1061.72253256505 815.334716938726 0 46.8488074208371 55256 314957 91 36022.565250131 17993.4806702995 10480.4189531194 23426.9204006011 20691.24366392 32277.8786769269 36317.9220550616 65773.3148845307 158571.164265171 1955548.92817515 136271.702682359 77965.5215894013 45924.209044409 30122.031704311 15451.8433207246 17892.284857232 16053.031885892 15889.2028671189 17993.3371592512 12432.6785325358 19973.343258731 8717.59040074987 6801.72454505287 5619.84672714211 5941.91458320284 4688.25078496773 3130.10852596472 852.312930066368 623.971657270104 0 47.3045437950687 55189 307101 92 37129.6581880735 17919.0452104194 11306.0971977081 26696.1848496381 22262.8671014062 33649.2903593419 40933.3491939333 63399.4982537695 161765.165729968 1961678.59964549 136354.255259931 69466.5135509552 48022.0944411867 35252.5627473638 15399.4086943933 18540.5885504893 15804.1147664779 14969.4310333259 19617.5144249188 12775.0136995744 20788.3660672401 8436.55187690943 5682.25370810488 6151.43000980027 4027.56307941332 4254.73129576655 2385.74104458434 1792.05681797586 656.829008073363 0 47.6671143776251 54850 315568 93 36320.2224722994 17146.8106264472 10283.3794434486 24963.0309391548 22795.4971170269 32050.7686221588 34881.6695354427 62105.2694310426 173448.481784489 2091024.49265777 141015.111948567 70715.9668808083 49361.3233808325 34927.5554862363 15355.3915219189 19022.6423045733 17946.6889018673 14608.085776427 17226.8506815841 12764.5635658867 20505.8213652029 8903.2961132233 5901.28668815503 6725.75445432425 6218.92234144938 4731.45456318166 2433.83227846238 1459.89141974195 862.889683447162 0 47.7904365854746 55665 322672 94 40431.5764916259 21175.7516576017 11976.7450748013 26700.3843527673 21024.3737759025 31482.7339044188 38931.9442369412 56605.3227772219 161484.022804294 1949626.96184045 144058.070764389 71374.7483505086 47775.9075641782 33761.6385688195 14321.6059191695 19286.7279167611 16261.7567590571 15226.3492218054 18542.5699796295 12772.9022115576 22577.6329756739 9195.16064929764 6412.99226970752 5808.27189949616 4503.52944112682 4716.4153542457 3069.19292764697 828.236384614817 911.018555508032 0 46.428879650355 55127 310534 95 36808.9198525308 17371.7951039968 12178.8344030488 25084.3330994347 21327.8508136478 34982.4261946916 36208.8985213308 58492.0165069306 154835.008225387 1974600.35430828 124868.271418168 77359.0862011655 45888.8746570706 34808.1016585516 16551.6670614614 19977.0574367542 14193.8078932162 13719.3735031383 19449.5201306427 11957.4328645476 21006.8930826989 7844.12731672133 5642.16013089266 5945.32586068526 4490.00250633321 5042.96296694641 2428.48570566771 951.333023140909 1021.10698690093 0 50.738338619909 55012 311621 96 35897.3735716177 18724.2137708485 11270.0052174122 27065.425708614 22506.041200442 33984.6848852506 37557.7561697972 67226.5764553158 161759.640465322 1920093.73379748 135023.617299731 73590.1863358267 46931.4260492517 34136.2045097246 15396.1036107478 18257.047015216 13401.6301896744 15969.9562471391 19204.5508097376 12254.8027076069 22253.2428063725 8325.05066516588 6515.59588311187 7009.61423898496 5206.8504021916 4666.74020222663 2849.74910756198 768.027585912056 395.088229010149 0 46.4198405953422 55637 315493 97 40760.9489538124 21352.2204862972 11120.3188690243 30666.0639729603 20773.9050390732 34105.5647235667 36382.3600198134 57430.9658826249 163072.477480775 1903433.92114615 132670.904168707 75203.4835443887 45210.2531376738 34075.4011757734 17904.0891681936 18801.7187234921 14948.9526158398 14779.8171428019 20715.8972224827 13827.3264455585 19661.3334934696 6599.2579776973 5484.15689411927 6334.90238605974 6502.44936117764 4396.36806214975 2534.46503682569 693.923074669826 661.218280024477 0 46.6942684474132 55297 318029 98 38616.0798624839 20329.0726537539 13211.2744239569 30710.2505954982 19358.2036794752 30797.272533764 36858.7433526431 62812.7074796173 159294.043539564 1901408.91630387 130339.700874373 74562.0554605881 49488.5866558455 32944.1610873778 17840.4147247676 18924.746261037 17531.4304310996 16020.4673947793 19028.1337705999 11580.5860436721 22064.9623725742 8409.45674655703 5863.3823914287 6194.40046594388 5186.79302688522 4375.09127330215 2625.48910215211 1222.83208479742 708.517864986245 0 47.4783309862696 55287 315662 99 39241.2429713764 19249.7908167205 10825.5244760505 26043.9699894222 20692.3724928181 32753.8000135949 37692.5401126446 69241.8634869985 157859.112617962 1915833.66891053 134465.321822154 74506.5208781522 48471.8938814529 35166.1244305096 18067.2562722944 19905.8290084237 17807.560211481 15316.6081242392 20331.7552997259 12232.5372662987 23462.1842101556 7319.67591399351 6042.28438893705 6533.51633414092 4960.14329518205 4322.99745733614 2439.16091211933 1282.96067852558 286.660177698752 0 47.2386113481844 54767 306635 100 38236.3035888583 16980.6145740915 10576.4445807553 30916.4944681844 21357.1672356946 32921.0137521622 35375.7486120733 59591.8252820928 166327.160899967 1872111.94992499 137564.226580458 76912.7494911375 49032.2278751827 37506.6544499301 17138.8496248844 20108.9639178184 17604.8005550727 15702.8552642746 19852.4582929199 12101.7509881798 20948.7828689047 8584.13621227471 6088.95335671208 7152.64705239869 5068.52472026174 5237.07284498689 2491.66463635411 1109.76343221704 1156.32980027804 0 44.4036665355475 55243 324178 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 37966.4675596094 18531.9998445536 11417.5320666778 27786.7468299616 21619.506444315 31999.1212527067 38269.6499057532 61326.2784860903 168376.318826771 1965659.74086886 135715.261799749 72963.429529523 47106.3608916399 33595.7205755569 16426.8263448748 18707.6896016851 16595.7357408826 15731.6714555062 18760.2832464286 12209.7171611641 21850.5020565353 7820.33304640562 6238.24583329911 6266.71498337639 5512.02897310494 4712.70375804757 2648.28902849789 1163.873154664 668.824771897251 0 46.6037189240698 55031.45 316115.35 DEV 1823.73600740948 1683.63970956343 953.844481174936 2645.13162138575 1262.45761393756 1563.72635886792 2117.53478571193 4591.15700203291 12570.8747472985 53857.304942312 4605.87527406694 3357.59802085769 1871.17336114856 1894.16553740143 902.260610308301 1245.70081658842 1528.06155955675 957.53718268386 859.318578572709 1014.69751629256 1340.29398123901 803.217127310019 513.684288895494 581.413838896436 589.463231945797 510.147078480517 428.90606541616 277.017422409764 284.246723690616 0 2.32658692927183 315.885434137125 6592.88790497002 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-178_preproc.map (6574605 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 16669.1069495318 29071.9875864159 13894.4850872799 21803.9192235417 30435.5381940331 40291.7528525107 69753.987071557 52098.5424858658 114248.763098362 1991196.42428242 113018.204379173 106552.257028699 83562.7513747599 33183.2616606531 130349.639766169 20482.7860776324 18012.3809651296 38955.7139643905 21260.9898091721 32101.3097277496 38871.1203784146 11692.8909772231 10256.8291926203 10814.9322661642 7736.77975532477 4473.91818851205 3496.1438779266 1411.50210213057 379.131929207873 0 51.6584311868113 55722 331816 2 17877.8546497017 30316.5950769697 12237.5940008629 23840.1317302563 30064.1968285175 45980.0245657206 72294.123489735 46427.4498301086 107837.567421037 1901385.43007856 111309.222708447 96074.2056776438 102414.0494038 31241.1946041167 127213.657122593 24220.7611911284 18723.948175199 40564.3772341334 20724.8025990182 35716.3761699219 35901.6955773121 11998.6267402837 10761.1177034959 11765.4510656574 8557.38245929981 11306.6935546497 3599.9500260876 1102.69894298596 287.540708868212 0 50.5441417993436 55592 329678 3 17532.972647807 27610.3021860681 9738.19671547677 22233.3114208864 35949.2420857783 44722.520494052 64738.3632515395 46677.8967882343 97845.2202060674 1918049.58155247 116409.29782109 102678.5691998 79740.436519838 32961.9358307837 108029.720172616 24803.9859384412 19096.8493736032 32426.1012910192 24458.1933777209 31251.3380464117 40717.212510469 10063.2895100788 10103.6651253445 10523.3823876182 8259.00858131179 4702.02380663459 2836.87355328463 1019.16773149452 389.131041207649 0 55.791985044562 55356 326351 4 16355.8858895398 27729.9913945748 10194.4580494522 16662.4822985473 28303.5405519329 46224.8295153522 66766.187586919 49161.9905321877 97167.536657927 1882895.32581887 122390.022420897 101861.548898584 80718.2023453276 33778.6071436717 92344.8061195903 24947.4584879365 15436.3552108152 42382.4959039533 21493.0576286793 32652.6991898981 39252.8781616725 11212.6014860659 10097.4940645164 11200.442632134 8134.82780443096 23913.6795569853 3589.52600518643 1063.52026892826 600.83895611485 0 54.8953378267465 55455 324193 5 16041.6513184823 25451.491121957 12308.3913316691 28913.2342248352 34567.3426870043 48150.6174794588 67033.6832425249 45026.202859773 97142.0530479766 1932159.90950279 111734.383109716 102560.334187235 90372.7312582765 32293.5166877745 88197.6112600836 21071.2955196385 23790.2158445917 38962.7548354452 22535.5075319589 37630.7309453379 34587.36443123 10537.846711512 9291.14786306613 11084.1329126097 8641.9149639944 5472.74087806693 4082.49146623153 835.158511738926 334.616478080451 0 59.6439734634901 55697 332791 6 16011.9914974593 39927.4085124765 12041.7600544232 19879.4122493902 33892.5809659233 43160.0517960111 66429.4439418902 49202.9400596713 99268.0138776473 1843184.84031254 108082.537331258 97092.390509353 86089.3622346283 31743.150671515 89242.0208776705 25570.8198070808 15788.3816194639 29769.5726095355 22516.706679964 37214.0897180104 42979.7076030613 10416.2440519115 9690.41765153528 14243.4784120339 8404.40734463039 5187.07113519502 3922.10962415159 1046.50896959034 636.580509350151 0 57.6174987870927 56023 334510 7 16702.5354024805 34859.1088504951 11890.9157763954 20658.5667212118 31853.8419572629 43658.1322969581 70867.6787751057 50166.142714066 106249.989152738 1931416.32386188 111630.66809027 102185.714940626 92981.3193883948 34666.1039880156 98446.1831086931 25912.8696939598 21289.7973537669 39340.9326494984 18369.3178384133 37079.9336495721 46321.9360604355 10909.6183128959 8806.4185003047 10684.5121492501 7417.76325566752 4646.91380637912 2962.62378324422 1144.99103022704 351.49337538896 0 56.2435227324979 56300 340615 8 17611.3436594839 28849.1041441305 9808.46856572103 23304.5616695046 27679.7122052979 41223.4985038892 63149.9197312411 47812.2618787381 99343.6176137671 1910672.0363633 107225.457802177 92202.7574764011 86559.4330998159 34497.5915217022 136229.512183661 24387.4598704298 25096.006921566 29010.3431849294 20360.2471840319 35840.7742884574 35361.0041219675 10492.5676199772 9134.21254656798 11834.4357818148 8172.33967723124 23157.6959302178 4045.13125575252 1055.39275895838 537.243640117512 0 52.4882454973043 55720 332234 9 15619.0390423031 24761.0120770164 12089.0332928214 25357.043072047 36386.0397606452 41840.2822417002 65187.4993802953 51007.8079066391 102588.718056729 1990148.23166734 111752.350917112 108830.449342739 85116.6246480021 34335.27333225 92730.4039657836 20852.6100128432 18386.3252356111 33999.2214997661 19827.6355220962 35355.6942047378 34933.8803664569 11103.3668627141 10389.9348784281 10711.2632957599 7767.618319783 5216.50476577721 4415.3888887014 945.127134246717 456.045141295658 0 58.5042718953899 55331 328668 10 16754.8432215692 27441.4219999497 11134.0892253833 21842.9944528986 44756.8349909611 44752.5469231976 62368.966938363 45956.6054343269 108572.418794658 1897850.58374413 104411.513586987 104342.160259391 78871.4755745223 31365.9806363917 120156.906343379 21773.378929817 18867.0372489659 35703.5147275971 19458.2339649748 33701.6051005776 34620.6533886393 10972.8471827978 11566.9666654055 11064.6955426613 8574.46221716884 4902.99753844825 3573.88981967629 955.002254563716 432.19058238787 0 53.3890873046838 55965 328270 11 17321.0824294828 24400.4781003935 10687.8959524048 19743.6376315041 32453.5792980497 41916.5781199933 66635.2944929196 49526.8348249483 98908.4812956466 1934095.92812271 121057.978830636 100200.974810335 84035.497453532 32080.3905516885 126731.99386004 23426.0819791813 25873.4234537675 34456.8470032563 21493.6188890512 32418.5825984973 32933.9665930779 9897.17154533405 9121.2347524014 11184.5243989196 7754.79174173424 5115.42633734887 3295.55994313712 1007.7388410974 415.572779785242 0 52.1249983398898 55856 333595 12 16272.4998174866 34345.4699781522 13058.5411296255 18790.4286311707 31355.9983576813 42124.6225311101 61738.186216421 51330.2021755692 102082.784974376 1898405.37270845 114950.22094199 98576.3304781881 77599.8234727334 35041.5056393155 145087.880708682 22659.1828983323 16640.9579923868 36488.552704896 19997.1764596482 32982.0350111607 37069.87269432 10024.5595097797 9416.84754667804 10734.5268369379 8339.47565048962 5330.59385516012 4090.35933764435 960.985329391464 495.758187745483 0 49.1885314468992 55452 330942 13 16685.8310268859 27160.8876913865 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22197.7908794963 40231.3224226501 36208.4974799126 10710.4974537983 9846.27784564676 10914.4552508176 8096.51635078038 5185.14038319094 3403.71793633246 973.465459104333 377.177985804691 0 57.1038608142289 55855 339288 80 17816.4017962336 28566.6897277644 10617.7175221077 23590.8194510775 26818.9850612971 42395.2605289553 63986.6935051545 46001.5504520186 110718.568431704 1897334.67111639 113209.639000939 92809.5716309279 84351.1337888155 34723.3829276105 109653.537541816 21129.2780437907 21358.3877808513 36268.5524993917 21158.310940086 35938.1548346294 40132.7647740401 10954.3972298698 9109.8585027296 10947.2490255361 7582.85628707616 3972.97449022731 3933.29904261529 1091.72056189163 346.164319962589 0 54.4202672929697 55071 325413 81 15394.7773473132 31035.5444363829 11292.9808189865 18977.2059379357 40133.9228194818 43441.6174734943 66417.8910665777 55490.8780676597 105811.433503697 1974647.6545831 115972.306828738 92922.5916869664 83014.0503389776 33768.405192782 103105.369847122 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86727.6723050172 32414.7888444913 120392.268826707 27374.8260863604 26264.3678099947 32300.7069391129 19798.6891111423 43783.358996637 39266.0955028128 10582.9169598006 11017.1879424737 11373.5933357694 8470.47491207165 17416.4474971039 3284.65624174222 1032.02881869318 457.4270933808 0 52.868910875125 56016 337684 84 16552.65989608 31650.1689011357 12836.7971229243 18357.9940936311 38772.1805247205 45789.0639984221 70654.0630083234 46890.3663142706 102371.914444951 1909382.05872252 115422.008494942 103423.70911632 87980.8959015065 32135.7810683412 126928.690014556 21613.7105750645 15538.6578083928 33904.9485389096 21193.0937251532 45386.4612284164 39773.8491122506 11405.4601773987 10691.9324301613 10911.8577880831 7872.55137596214 4688.90373329041 3814.32544462741 1108.68394421416 454.195665820927 0 51.200874845043 56154 334834 85 18839.7133203843 31160.2622851313 11785.0880393712 23123.0691824277 35858.0031023858 43528.5016966742 71874.5477230246 50337.2938975155 103710.123429668 1896998.66073383 112069.075275452 98658.2335913307 87756.7108933553 36012.4962986624 100924.75216001 24106.4330401254 16754.4234996135 33249.9996400889 23285.0153338273 34676.0944321211 40768.6010806017 10574.7623129928 10046.3173241621 10593.7808573008 8805.51534578621 11622.8420114467 4476.80598549381 1153.88574322603 556.366178451687 0 56.1485286330272 55318 333197 86 15205.4492217689 26799.9299467643 13521.8211896145 23468.8481873043 35996.5469735121 48365.3041965656 63400.0260789782 49558.1028997451 108500.929894386 1956915.34967558 119905.907965692 97064.4317999934 79575.9543023777 35394.9674037363 89717.1379646698 22438.2147637721 26826.1653957742 38307.4024616404 19303.8726709506 36672.5519427137 34997.8457119304 9699.83983828579 8747.83947681138 10867.4250858809 8389.65938083311 4542.86506612675 3809.78656048726 1096.76370574593 510.376296923443 0 58.915118943814 56198 338194 87 16718.1152864288 27720.9316192252 10258.1785812331 17629.6481706445 31643.4323363844 50224.2879024363 62889.8530674635 53608.2450998102 93764.6348119042 1922418.29302213 115166.271437319 98503.0096082072 98661.7118339864 33860.8328341002 129925.335710198 21850.6252638734 25590.1898942223 37471.5624991037 22325.5534890396 32852.4033280998 39078.1001707492 9268.07024245448 10013.9079912957 12565.8984280326 8028.82595833918 4421.94778840267 4420.29098452815 1160.87305552036 635.808681173401 0 51.7464886202587 55490 330833 88 16511.8364145732 30322.1213765949 12468.4150197109 19621.7112067855 28532.3537319781 46030.4151526481 65162.7450886488 49083.0480515172 98607.8086674795 1914987.44297754 111523.472049988 98219.5534153094 94450.1471533011 31814.3483866563 88905.6926217907 22796.5470890474 18185.9094667067 38882.2672672801 20977.3403748527 32418.5680633668 36532.4363792698 10129.9209579975 8665.13510221071 10921.4652517381 8264.1478995887 5689.38668517393 3511.0623528152 913.969307119363 462.75117356035 0 57.7648098018714 55104 327518 89 17302.9871880378 26048.6990092451 10250.522267883 21840.9299717415 40645.0881557303 50864.7226776264 68270.6761667426 46580.5967946068 98797.9615022858 1917053.84167195 113105.262114176 93247.1159965431 90629.6493764147 35803.0970675179 99330.6686945273 21942.0357905488 17725.7568530975 34709.0569712676 19519.1555877976 34051.3803616749 39374.4820629501 9929.80171715218 9287.59718514494 10996.6406459477 8702.45090899263 5720.93870594844 3097.91062922895 1097.78954170146 374.598047103328 0 57.2607866203397 56053 334273 90 16350.5845130351 24874.8933738521 10986.0978828751 16937.7671132611 35346.7759004568 44311.9044745312 64667.3908182184 49754.4911914663 99225.5785444889 1929957.80016108 115091.07431569 101040.673649183 87769.8232774951 31223.1418907522 85271.7806292046 22069.2293414513 19310.5955504289 40407.2683717564 23462.515196721 35903.4955088474 35716.378948687 10767.0330497958 9865.6968336574 11036.6668052189 8681.33758403358 4159.45210708379 3404.03674560808 1489.3184420501 509.952668548574 0 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2594.78281491533 1184.56024681125 541.95874780036 0 47.5907579030644 55762 330870 93 15721.2891653316 24261.6921933177 11888.1820949757 23899.949911318 33003.7149477276 48279.4210475608 70078.8905350385 46213.455487082 113368.904966988 1945666.05304465 119683.333215754 103645.663919815 79696.2679181437 31363.2382249855 104022.228769201 21293.9975508131 23224.5075922622 35471.8443781529 21049.413514228 34877.9960834898 35632.6288569734 11472.7186870978 9936.13171531221 10873.1183055048 8609.4786631418 5079.94967568313 3816.33650317607 1008.88554082588 384.008462898869 0 54.0122297791996 55938 332030 94 15588.7474569748 32528.9037293008 11052.3931251684 24136.1163173686 32556.3681175859 41371.4423252338 57597.5205828863 51044.3984219045 111273.536874316 1919798.36909113 115936.459660691 98425.919442609 83049.9815130232 32964.6291515003 112650.881173644 26878.1516632978 20236.8726445516 40461.1923661699 21251.7310063156 32690.99104988 35922.128504263 11301.0205032588 10203.0035707412 11336.872283803 8013.97010408814 4440.71971597778 4168.13950384958 1061.72743755203 433.769982701578 0 54.3197714671224 56182 335287 95 18837.3959144938 20450.94899402 11569.5443580011 21722.3452880485 30337.7882202942 40024.9726675624 70350.9654930694 50088.469767249 88648.3126555354 1877281.77985694 123139.602123655 102473.323679674 87419.9206777736 33050.8738179228 104010.855415399 27532.545456944 20651.6019359063 35362.207871838 22007.963036793 38906.4751616705 45730.9931267351 10727.1413044262 10429.7880861482 11174.0922481403 8122.41238162513 5230.11108260323 3297.10964219002 1424.01729566389 638.650824134408 0 54.0640187458294 55955 335346 96 17466.1144366305 26697.9299910681 11518.845539763 19520.4519552344 37836.6204232652 45386.7770431272 62704.7175832807 46619.2441247311 105084.109070625 1950967.25344081 106616.435136952 98635.429264736 89464.6788489256 35868.6320904979 106515.685427794 23704.5577949236 16737.436801518 33813.2901708318 20994.504943746 38927.0752197489 34247.9452304087 10935.7791810758 11118.1931620817 11297.8855177192 7505.9024176912 5136.76047208623 3761.05286544533 1030.04365058451 384.404202780821 0 57.2824599650414 55276 326399 97 15217.5785905978 32269.7340905093 10605.3196688177 18194.1560898554 33411.0981628993 42915.0697655983 63500.0997377951 48591.9254205455 95426.8231028177 1888989.43427971 123547.050399522 101004.65951182 83922.093756425 33198.3292515011 107386.361185723 23941.002670277 19497.3278101698 32019.43231093 20141.5227729772 35671.7409381971 34203.0905862409 10438.5799668198 9538.51742299839 11990.7785428227 7337.25825141154 4191.43866562287 3873.79497084135 1197.72560727676 629.878684864129 0 53.5465404422947 55487 330839 98 16957.2682537454 27333.8551978429 10350.2677817349 18466.8691114475 31830.8305571611 45074.5908829771 64352.7786287583 49292.3780405517 104778.840992427 1874325.3149603 111770.070358009 106150.735031736 84157.6715821978 32967.3278980438 118550.64179661 22269.4568150826 16205.399782985 31803.3409714363 20722.075991032 34370.0907277982 35482.372161271 10682.5064408594 9699.92145526471 11394.4557401272 6543.0896138083 4159.56985084774 4213.00570831949 1066.37696575342 267.626454661387 0 51.1200320069889 55875 334637 99 18225.2459795574 32776.4658469379 10083.1945736687 19839.4367189426 28733.669795741 44778.1885449944 74259.5725102884 48478.9167954766 100929.987952067 1892900.53412508 104964.841610019 96862.3563544992 83755.9620551127 30978.7015065786 88943.7750705315 21685.9688135061 19328.8852520582 34845.9719295509 18580.7404672432 35091.3453735523 35542.422468061 9631.00258144979 9922.76300169389 12088.9695388558 8886.58289981381 5269.56147585475 3497.36952423357 1229.67883855061 317.920082020014 0 58.6194467592479 55473 332413 100 16481.6238170071 28588.1234491185 10002.196853548 26480.4015661214 33576.7314599453 42386.0941741393 66227.7893677066 47535.53806315 99908.5242461103 1914155.09872839 115363.220400483 96189.5595192161 91148.0700725199 33666.4297309442 115850.57533243 20130.7469392589 18600.8904379842 33162.9966957175 21556.9750035102 30279.3455109523 39397.9640370709 11030.4387592634 8540.37398787107 11358.3567824111 8930.45358639919 4489.52287222712 4371.35565703365 1324.67582085812 347.284488787661 0 53.7189746848203 55693 329272 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 16689.1636352346 29215.4048151223 11287.0607530326 21173.3751937441 33479.5100981105 44573.6282256446 66860.1745791398 48643.3925971354 102486.877841444 1915442.71957831 113740.089580578 100446.936381933 86134.3108016282 33174.2963889873 106937.500918198 22281.4096806548 19882.2084625718 36297.0333128814 21016.3610364726 35216.5276465079 38765.4751900377 10497.9808805071 9784.76247953535 11030.0421292784 8292.34231356044 6259.46804824929 3759.66201380434 1090.27787820072 430.812353405806 0 55.0498426424581 55820.98 332973.4 DEV 1481.12564003583 3755.896858152 1427.2970717739 2600.30128614139 3184.78817412593 2687.60564052866 3051.61344292576 2620.72250462954 6815.95173389282 39135.1199752289 5035.35888368091 4503.24053175818 4942.13642040665 1444.91247981134 14865.1176622356 1905.89206475353 2939.11215997984 3558.59442641118 1307.89365307723 2873.82210999036 3951.18943490056 731.474087343665 706.162144917125 801.977310141935 608.429242516572 3598.52146919719 470.618595440964 147.358526300092 103.584794301021 0 2.76904543653185 355.282140840206 4353.09233074604 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-47_preproc.map (3418106 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7023.96144812707 8651.41124561968 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7115.66588457331 8119.1992094863 8515.78334841437 19731.7861437407 88128.5752801149 19234.3938272052 18434.8929840798 39020.9629103836 36810.1131379982 1021151.72926459 28098.9322471189 28682.7265994904 26555.5834065989 29567.1271908276 19917.178393595 69464.7663765058 16040.4885720075 122191.634173581 14568.7001616069 25504.0211981445 13695.9425308138 9186.88118578481 10580.4997525257 7472.2466874078 7403.78861951685 5990.95436427081 6004.43442686794 4200.28085188099 261.797013354443 0 34.2782168210946 31143 231363 97 6358.44299072653 8464.8149473035 9997.88458291849 20399.8863626712 82844.0265107572 23673.0897961841 23198.1861077357 37963.9263692671 36166.1217741669 952932.588844476 42349.8209048721 29663.1881854921 27422.9860077216 36473.7687840845 23622.7547858405 76472.0136620218 13101.1166816051 116419.932559188 15684.5307418931 23409.9837906319 14267.5017090499 9944.47157024824 9218.52330055367 7702.24642306393 6235.87160569844 5232.01163124592 5770.18339558199 3188.6474801106 260.80584214538 0 33.2147062846688 31236 235860 98 7001.80457454397 8299.17482191192 8280.39457727653 20519.7606449325 69591.6678088317 19424.1096614361 20370.0330013164 36840.8813825521 34548.5171456106 985599.384132662 37073.7987320875 29152.0282870733 23778.0267252951 40399.5109990423 18895.3609273231 67729.3355718224 18264.2653857991 118431.238768136 14781.2259743638 21054.914541819 13129.5851849801 10153.7685816913 11703.475083164 7290.50054378883 7973.57768331095 4914.24939989182 6306.6156243418 1576.96469033072 143.760573711638 0 36.0150017148215 31208 237778 99 6175.06998932557 7205.27626959118 9932.55504653404 24779.7055692494 86041.8953253368 13815.3437411879 20388.4465888368 32669.3166429834 32729.4841562091 893347.926304665 37442.8291158393 26956.335530988 24092.9618662072 39164.8124254195 20946.3474347394 68299.9410671293 20368.9632908636 111979.607301697 16101.3602316686 26660.5348558897 13533.9250144809 11585.438170568 9487.91617606351 7024.38416769679 7543.02118918455 7115.01700365331 3739.57900754657 2318.7607696608 330.192975007419 0 32.1441528950567 31086 230387 100 6995.16753824076 9140.94918120605 10372.0128483535 24152.4518390053 81165.2254291822 15764.1898745891 21687.0641380479 35164.4726703828 31785.6950805695 914451.030668581 43551.2030059645 33763.8348559122 22221.414225311 40997.8849089655 20678.6793347683 74139.7998183149 15699.9854579836 106411.72131107 13323.5979086093 29296.5241561431 13249.2420155774 9606.51800953159 9519.29609742667 7821.46288633344 7984.54634518087 6390.21125397357 2726.83827222441 4153.40360767585 243.823311033622 0 33.930496101473 31770 235779 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 6772.90558734616 8340.11286833236 9506.04720080904 21059.4850157269 80046.0004127865 19552.6935002083 20415.2770500114 35988.3220110809 34008.9869721093 958670.936955792 36491.0958952225 29453.9007251525 25850.0212140657 38519.4442339738 20892.2804711319 76872.79529607 17263.9942100403 122983.54812636 15366.1606298041 23708.1469139468 13767.3518421341 10364.6686579864 10556.2688601558 8068.86411628644 7841.14868186743 5860.03192046869 5631.52809223289 3553.47458653852 271.47844457679 0 32.3080640396887 31502.45 236471.39 DEV 363.427188190073 550.705126086895 1302.64830183005 2689.77787396793 4900.77927365934 2862.59311913462 1533.3797801994 3270.3121423226 2401.11603636791 41751.3194030714 5806.0204678406 2155.31299325567 2685.37212876068 3658.85279492962 2430.99480645107 6159.16499745233 2299.30356167888 9049.89845325484 1946.88031861737 4013.40946133499 905.984098914396 1072.01676421958 948.266879052569 1355.2011213012 695.886460716495 1038.11601373935 1266.45104883216 1077.07049767003 63.8837424817269 0 2.25204899779656 295.611040896648 7262.84434074557 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-48_preproc.map (1981064 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 5870.90180983316 9454.08473481914 8209.76120840471 19713.7134606962 12959.6732124058 12462.8956516555 67461.3116468218 35670.7657330081 36538.0235280378 911881.726228385 111318.905253213 41226.5102628569 48063.3483482049 22390.3251691237 22750.1052248906 19161.2780814899 19975.725019611 48861.2970780639 12267.089141723 22509.5403214321 33477.3481745471 10194.543651297 5492.34518704628 5515.32880885901 4944.74225890091 7774.93991237182 9789.43966247179 1369.94616679325 232.566283898182 0 35.4049761873048 26152 230887 2 5445.35062343375 9467.0949525345 8492.18515359154 15484.4117379032 14481.946380837 15924.0796226015 39791.5161373189 28132.3757357059 36035.5547826196 922789.712180346 103653.53643595 33959.5775514187 52816.7959064721 23524.4388995608 29677.8330430801 21156.5109795614 24963.1236914362 47234.1523805342 15064.2662597638 27119.081693147 38221.5892759206 10402.0638914423 5483.766880793 6743.82080934046 4251.05514830608 8719.93362020221 11120.9569942478 2154.73941772631 403.899754118639 0 41.3133148345566 25957 232785 3 5078.34328101278 9740.67943457652 8601.35985178286 16531.0725616385 13115.2947802425 12964.7350206086 40991.8809155074 27074.8655388679 66896.2984912507 1009632.66033113 109043.587479593 34874.3013290289 56428.7834208734 19628.1824649725 25643.1838103375 20036.2406855304 21702.3219090505 46798.4758094957 13633.4460844545 22327.0780524841 33393.0496621809 10404.1241584817 5809.9594625957 4069.04133319261 5089.40970733121 8353.01973119352 7241.15469122597 1865.1744424471 341.56642225569 0 39.6267283213253 25771 230501 4 6272.2189271483 10583.3240187813 8074.922945126 15142.5577879984 14844.9849296311 15305.3519302252 66350.5890985027 37372.4954616322 61949.7149703917 1022239.16592789 87123.8800482017 22868.6296144695 57125.4406989424 21084.6676051167 21712.5551236254 20505.1803571424 23370.6277369992 46112.5451722769 14548.4688494584 21702.4477481724 35044.8018357876 11049.6459698727 6057.37503779752 5158.43536116262 4960.05696625133 10013.6684774349 11857.6769036705 1923.79871329481 147.372299766325 0 44.6461374663731 25918 233366 5 6337.15391890977 9877.54541735918 10514.0805776371 16350.0600955733 17119.9353635015 14346.6234094754 124236.805790614 56131.9174003953 118487.143021843 924252.585952142 79837.3533907571 33524.0791041958 53056.7391803783 21821.7423277523 22977.1486318751 18700.2875677805 21982.8993401964 47234.0888174575 15732.7310029865 20948.3412285209 35878.0258513696 9914.70343245548 4942.73219949477 3809.64597953495 4316.28251529964 10175.9279238903 13126.3624694527 1834.28577442364 197.904325432641 0 25.9273978550098 25695 226101 6 4913.04379691055 9883.49383474245 9690.29627800872 17732.6582045139 19752.4578105392 16285.4119068579 94071.0167507234 44932.1991877181 114868.485192943 1078613.38198681 151371.606378271 42601.9864875945 59744.2774429668 22190.5776055491 22746.9366631956 19303.1095230676 23111.4054692864 48590.5979290143 14846.2780599365 25975.5440226362 32020.9200442273 10136.8883810421 4903.28385274226 3871.65328425975 4204.15023636867 7147.45683417676 8612.92987616097 1629.6757042939 190.22431523829 0 26.9988163857722 25761 227870 7 6557.1295549371 10897.5906729083 9572.05320340044 13628.91601946 15460.5748277243 12173.2637259544 37528.8208124005 25659.3156245811 35228.7875623623 915759.71272247 62269.9481394195 25500.2839551665 49171.6197473731 23651.7727620687 24311.6582124888 20051.3254409162 24110.1845423429 43357.2180838281 15341.3582103808 21397.8376957351 28469.6161682373 9380.71016645354 4808.39181760575 5119.25573022499 4262.41299812003 9087.52947179112 8960.79192105877 2444.42898951035 177.164129116228 0 62.4248715738658 26089 220657 8 5680.31678112519 9135.31844137558 8989.88145068161 14998.0903598331 15822.5101644204 13702.2991454455 122499.725944819 53773.9049805572 117983.524924489 1031758.54119498 81988.1141460915 22484.9335103839 49431.7777676105 19308.6714507812 23183.3734313798 18622.5645102668 21540.083042767 49732.1908394038 17049.0038775348 22514.7099730384 34290.1461414644 10272.8150764353 5544.76942331627 3609.66287338656 4666.40193152749 8337.33797113808 9178.27849423015 1494.10967604841 238.87487162707 0 28.9965090076136 25739 225008 9 6864.2750402303 9843.3463041962 9528.48949139212 13221.3059646913 13841.2522871601 17082.0037717946 121548.846274853 53967.3179124925 91882.244100361 908740.524085682 105668.403839692 44890.5539973142 53220.6581218444 22156.196637293 22596.0282427569 21983.9124540761 21753.670239728 47785.5061685448 14432.149856087 23333.8270169939 33022.0306859035 10262.7166689985 5567.26570686764 3306.1583393259 4357.31908485303 6999.71751615105 8939.81837752108 1852.29815652625 183.85469824199 0 26.1529759501241 25501 224189 10 5171.26001427349 10290.6537479572 8953.95925791322 12497.4858152757 14600.670509668 15607.3674371208 40662.3676262306 25237.9128845804 59403.2821668524 962494.210841209 93372.7018657317 32735.4226692912 56352.759552987 21059.1491736243 19083.3250884629 21615.5695781906 22156.0586690577 46542.8161201698 17500.5273533668 21321.3241946845 31055.0647677355 9531.5300220297 6509.64388495687 3623.40436062539 4638.61887847249 11157.985444987 6779.17967005946 2327.33306961777 243.59406063792 0 43.5948646050943 25665 232646 11 5912.07723952604 9506.19919351591 11898.477628816 14802.2838418121 15680.0830446488 13092.8012854118 37473.2563582237 28236.4335892794 117937.415960171 1154267.39389492 173993.092329095 42660.2535247803 56948.6761515335 22753.3553011977 21743.7158998832 18840.307032988 21588.4207392619 46194.6851010392 18469.3333329159 22421.3475443319 41196.8448812251 10290.4291325594 5214.80763046065 3539.60703360552 4415.31600706757 8419.91757498207 9715.35149089317 2485.35662915141 198.010548702332 0 27.5135980735421 25575 226158 12 6356.30724818271 10374.3347713465 8868.92867783011 14593.2255942491 15142.3712932604 17036.8349945082 95777.3519584456 45000.8072869152 65936.638047536 944824.240857155 79873.2536873395 36449.8127412401 61619.827536254 23882.6033166241 26860.2590644838 20525.5280379598 21813.3299845325 44858.7834279237 12641.112553885 21989.7419622977 42565.1311655581 9374.41192157713 6674.69995188039 4953.50265831008 5069.78682540294 9677.05533345678 8682.07847491673 2117.31447033674 265.947575605167 0 36.2889485816308 25819 230197 13 4958.6640420863 9088.31759364381 9215.35863917086 15225.2554966818 15630.6513165961 16130.2795252359 67150.1697354412 34650.3614319632 91321.5002705257 942569.477466463 143158.201871109 52631.5917545073 51741.8265840272 20060.9599951502 21332.1832438341 19577.7212011746 20769.8206999186 45703.444218338 16414.0074116891 21050.4117724072 36312.6013524847 10738.2014074439 5584.23830120219 4057.50583766944 5607.08118951635 11212.8110547682 9750.56648086186 1937.16307968722 133.251141311765 0 27.0541770355446 25574 232976 14 5393.37105590213 10711.5178537711 8722.89026340782 13750.635158654 13545.126254464 14614.6462068499 40490.2335129747 23629.2563234447 142131.748424978 937978.293510847 107406.046415713 47308.8760170411 54769.5942841701 21144.8499055949 22999.4132900862 20219.0666053138 21386.5073330998 49123.865503108 15871.3480159205 18227.0522766455 31480.1492815497 9399.79352655299 5177.14694877 5540.97354186017 5028.02041031419 9067.10673087774 7803.22297228023 2363.78911809429 127.888200376126 0 26.270689358247 25922 237643 15 5576.06542777487 13168.0903245556 7163.20964203796 14716.9679630995 14085.3873104175 17149.5143793036 41144.06353985 26507.1570114018 67864.763538336 922545.213830546 93225.7514648912 30937.0418429397 52922.9749155214 23054.1055113468 28090.3188128333 17598.4339540034 22416.231123272 43204.2317743777 13640.6397780876 19635.2520193373 32033.5271926314 10790.4490896357 6494.96750546555 3940.06752441986 4428.60377401998 7748.46980470946 9592.48938844628 2144.65070380785 296.709485348256 0 41.1833958772019 26537 245085 16 5989.25792533842 7212.21201489936 10016.0704591034 16930.5256325276 14851.3928522226 14717.9375484076 94456.221684859 41789.8572501046 34074.7069793394 998111.430743058 97860.1297056544 37388.7916397575 52478.1263561917 20899.9769495424 20786.324267528 19841.8736312615 22703.8012988507 40050.5963548734 16484.685763127 22771.8664035932 39972.303342995 9957.07355120004 5023.80675027985 4696.88733924374 3861.71432825557 6558.35547575011 7269.82195007446 1557.33488629774 288.556605816605 0 38.0981875420039 25693 217015 17 6145.0453278254 8454.42506988006 6907.41409729033 17155.0588252975 15220.9607803537 15038.1828971019 90815.9197213383 44510.306626322 42056.2554753016 973585.753302678 70333.1204589257 25453.4476656481 48556.1305585386 18967.0855250972 24564.9730564353 19117.4298404461 22086.879017601 45737.0249971979 24100.6702882358 22243.7882806562 31917.194865514 9801.52227715601 4956.2406835539 5939.12104396399 4361.03043351503 9371.24722248807 7144.43464110881 1346.22309918775 227.370164962894 0 43.6169725139263 25628 223503 18 5986.25697961602 12915.5151144223 10241.5646836004 12499.0281008763 15182.5941848149 15155.558133527 35837.8797332986 23729.9681145524 37416.1222294527 960063.175998228 101619.986803023 34689.8139294845 52523.9964423897 21109.2567828984 21804.3685981019 17669.4224306193 21070.5430144841 40401.0997369673 15626.8232986756 23255.9683443033 33656.8981135508 11276.8549066818 6083.49717111855 4419.30373094003 4839.81779091756 9012.10299272981 8630.18567637078 1626.45597404943 152.257742369634 0 44.3822572585113 25799 219826 19 6835.21313636572 11107.7778594215 8396.61074865291 14906.8451513012 15306.9466753702 12487.6509309288 95075.2146323977 41454.9279002279 91743.8725544819 969485.97673577 79721.0652786469 36277.2878833533 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25726 225028 84 7411.5481246035 9691.93243573879 9208.11683667369 16828.5881780606 14345.5036279701 14808.3783642419 66910.5207387586 34107.3956738479 62203.3786173398 1020228.02930319 107507.208231838 34224.4293343341 56063.7341162951 19189.0087009445 20495.5727451977 16994.1563617285 21875.2882254311 42988.6391367192 14712.6460695046 20919.2381265723 35723.6053308756 7787.05698481201 5728.68522228836 4210.1214442816 4410.68937002314 10907.8738848958 9361.00918666077 1966.71085399694 207.198545245813 0 39.309792495541 25665 230006 85 6566.85770700359 10389.2027475963 7714.41361198423 15587.0945485059 14961.5609750304 14449.366552017 65976.6669980905 37555.242917805 35958.5749484123 890611.793554557 44483.1028949495 23530.5645507663 47738.8928935249 20677.5999248551 21887.8260537271 21705.6583771823 22378.1384988486 43133.5127360569 17779.7305126998 21305.7637146847 36391.3891169196 9243.2042028258 5343.71576069275 7032.14436091972 4079.23370349729 8906.3743065595 7585.78524478728 1952.07950176062 133.90580083312 0 56.6210246279826 25878 219646 86 6661.72888400242 7294.88547092692 8137.56977940645 15012.8377066635 13680.0015875756 12134.2094533588 36287.3313459138 24307.4077992287 62129.3914893722 903603.294191543 73328.6471309787 32946.6027449785 49930.4075384617 19239.392934031 22698.1038530601 20418.1569378763 22739.9160344288 42843.6645101131 13579.506041937 22085.0725028587 32387.0234531697 9347.91524495078 4681.97217733927 4524.18451204112 4650.26068693517 8748.93431684091 4644.66039038115 1876.82992908596 159.923702384087 0 48.0577462160961 25927 231025 87 5604.94536528 7103.89927112294 7265.05805899311 17841.1423943597 15164.5803667764 13778.1791413802 173628.636980431 71502.3163779375 141476.837034329 949698.010803893 49934.8649139657 24917.9705164625 49210.5686551921 19594.7457307777 22707.4403051379 19574.8133676224 22022.85911525 44936.4925358638 14540.3204744553 22015.9581571633 39377.6895354256 9801.82938957177 5663.05848739786 3439.27770145316 4471.52928132945 7070.71290895871 8225.14426892119 1370.63321759821 430.178865513214 0 20.5999773472353 25265 214970 88 6656.58178588888 10721.5043433877 9345.07737359283 14615.8243885219 11944.3366351065 18151.565106562 67248.760609475 34965.8546735129 68859.5068424414 983454.200897041 132656.654781978 41173.2378747786 58452.4233952776 22611.685940683 24539.0980571107 21274.2170929515 24144.4386854692 46594.4152122494 14627.0654079326 25300.4640874601 32726.2978091238 10115.3525641691 6197.14905499256 5705.28389474165 4055.91144036607 7884.76381420783 9878.50692524417 1938.20212019705 100.240840197389 0 31.9163032838112 25798 223914 89 5908.81041749657 11419.0357861401 7629.33525229335 16193.6353974796 12244.1288661232 16201.3151884819 39085.6236783674 27536.7527857117 33565.428847272 974917.941648225 112246.022750524 34311.9335537082 53299.5836201428 20637.9534414648 20511.6127711304 18170.6332159539 19997.4385498193 42882.5436540788 14764.995524851 22439.5799684406 38831.2753807399 10308.4714075482 4730.17852946916 3529.7399947242 5142.19976368915 10036.5926918971 6339.88724956949 2100.17680770551 138.14954284318 0 40.6089950084371 25886 220326 90 5350.56794332965 10992.9745076362 7570.21687526407 17427.6503332347 14078.373438046 10936.6504812944 67914.4880939374 35722.6529666309 89488.4826657316 891889.623188383 75268.8617395614 34988.5528282131 52808.2357636204 24922.9621240219 23787.0437306876 19617.4790941583 23970.4610241563 46052.1354980132 14291.3844682122 23274.1924011513 34289.6644709524 9554.72738092662 4624.89252529756 4062.53351836297 5026.17167597579 7367.88931659435 10075.3603467834 2340.00338462784 280.69705023439 0 36.4852841575223 25778 232421 91 7069.70286627957 11418.1261241027 9783.45824668366 13252.0944349848 12719.6243571081 12327.5780801787 68454.6771859171 33290.2090527125 36468.1966834915 916420.404290219 135852.869112942 53610.9106674113 50790.3548226986 24048.90663968 24122.4643239062 22195.1236898974 17987.4758971881 45636.8670152734 16422.8921978212 19973.7333254288 35119.8873960185 10695.2536496753 6281.03313554269 5230.92675836267 5142.09960153215 11757.2777879056 8955.38158702429 2091.36889850641 325.815345864852 0 29.914340939121 25936 227120 92 6294.4587108304 10411.0157856253 9949.94660437257 14109.7473790932 15296.385546912 15589.5001815657 67102.8815067744 36613.504237212 94526.4343516432 995299.993429172 70607.7586512592 26662.678335701 51102.5703388483 24734.0743885115 20206.3433773114 20128.2013830546 20983.5724217529 47129.3648555032 14701.3586216611 21340.0257667541 37815.0322040702 10747.3567551066 5902.30702109244 4744.62564520219 5135.90526702619 10330.466125947 9717.00677383162 1606.56299558424 215.735137192564 0 40.824924827763 26111 229521 93 6306.38942474573 9813.88651508107 8144.98828181552 17143.4050422973 14800.945432816 13248.2720154051 68458.6686125511 33550.145697874 86771.7737462079 1098252.09560063 106433.759393084 23600.8533107194 50075.6190446789 22620.6237599059 23208.3603959457 21158.4150653355 22218.458080417 51438.3850220949 16714.5364434033 21167.8363238709 35698.0102545684 8056.89369498596 5401.84909557184 5143.99061296697 5223.41849475144 12748.8230512228 8016.9786370611 1663.92588257346 201.983413648757 0 39.3162756665147 26130 238184 94 5505.71507781817 8404.79567978513 8518.18331249844 15346.4365515798 13830.7466270737 11367.254239313 63023.3315530625 34519.918254976 61566.1385481 998566.061863022 124326.177402094 43098.1143317282 54884.5970601826 23800.6032246016 25000.9021517295 20501.0621981221 21023.9555151552 46396.6935771088 15291.2025513948 20022.770832493 33449.1063639445 10085.1673271819 6224.9487627554 3681.5916160734 4773.10958402396 9743.68075389761 6834.67060886036 2007.56435503473 236.35405061781 0 34.5551209626041 25448 215992 95 5895.43808883171 7693.71971017443 8806.05399886732 10319.5834944644 13771.9694383019 15194.3622412857 64981.0304408417 33244.1058608687 37581.5345259698 955816.502047063 70005.6939624886 24068.9892062129 47399.7371645525 21088.2577728873 17869.5809134512 19441.4521969309 23032.1786381 44926.0164394312 16994.4352322131 20318.4153902852 33225.6203630031 9430.46099938937 6005.90819548034 7023.46762570608 3914.44011000393 11222.2338448377 11608.6733417139 1202.9412683067 247.377425603526 0 51.1959716067887 25724 213774 96 6284.84062828619 9318.37756412978 7485.16517589321 13986.8432635766 15512.3126562196 11652.7061699812 41091.1175774327 27888.4413965635 36812.1179921463 969031.668092704 103383.664965495 34734.6049244302 54845.1280973456 24445.1961332193 20086.529857875 20311.5963038328 23172.4553793856 46237.8221214407 16105.1940831772 22997.8888866217 34771.6844324848 8931.48886890929 5530.27042746432 3743.46836824902 4650.37705982832 9430.81040519325 7775.7713766958 2562.02911069387 95.0169369101664 0 42.72076872099 26115 228739 97 6374.6367530772 9395.1834493029 7294.27857955197 13707.1515293543 13685.7752534685 13428.0422177176 38528.5534649794 26859.4420406689 142773.594537924 944068.737537775 120351.338735886 42766.6169914278 47571.5020991166 20153.8481249415 20889.9000689508 17865.2728127663 22441.4201058975 43206.9927254671 14302.0276142606 21270.367861934 30045.7343466867 9318.66036503777 5058.59709443444 5049.93645221368 4629.24215022017 7666.65491883228 12482.0040745621 1460.85676017053 167.129700032237 0 25.4302868614461 25494 216486 98 4333.55738401081 8489.8482061972 10713.3835374925 15450.1874318596 13694.6625832649 15225.5075656128 40443.6859406245 26667.3242546221 38909.7669283202 941319.591274407 54406.5316606172 27163.0812494176 54897.7232661259 21027.7417504194 23247.3459260463 18761.219557862 21345.8944596329 46894.8256448575 18487.200865456 22299.8851240283 35936.7620484538 8955.47446361394 5699.8768809458 3654.51362963541 4661.22562400026 8196.4558061347 9108.90901693111 2374.41922657131 282.572120232953 0 63.0181004817134 25633 217920 99 7824.42464773853 7705.30393652079 8295.26239460297 17814.3107471567 14118.6547055152 13168.6254155614 41677.2456916066 24377.3074941918 92902.5751621165 983674.621250884 99544.8079999296 32532.039079579 53159.4113923606 23840.1074682989 23623.8216284333 24174.6647096645 21255.527666769 44980.0694550576 17280.3379797434 23319.0044429005 33804.8605221317 8545.98953859465 5375.7965262079 3609.00264607372 4506.33123253448 11374.5095725455 6639.89109816686 1836.91362123654 190.876368643937 0 37.5242947520228 25622 220439 100 4973.98667642833 9224.84959694238 8193.82777796308 16474.948439671 15766.4172761037 13044.7644355422 64506.1154978056 33036.1170964732 89525.5252514998 925039.177785519 80684.3407028077 37827.6993447723 49895.3059747667 22802.7421121761 22077.3853918225 20649.1858746589 22673.1346878788 47206.4034204002 15825.975849051 20457.6601043274 34888.6637730799 9016.70647628017 5050.80224502684 4894.53759394563 4007.99173598878 9913.12950197874 8969.33920408352 2082.78136661148 162.368048124922 0 37.5091552098893 26117 229291 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5865.83246987811 9629.31831043105 9129.61318692518 15826.1496868507 14451.5225732257 14257.2646138857 67378.1002047323 35140.1839932099 79430.6862335957 971537.292199752 95048.1990009366 34250.822184866 53981.7802639923 22149.3629179231 22739.7404114112 19720.2739663701 22032.1921964203 46202.8484834023 15623.7162308854 21791.8282237893 35176.1711542114 9767.00192522416 5468.6143161509 4569.89776048758 4563.82358770422 8882.20625237556 8771.92387991741 2010.78610246961 235.381992889325 0 37.1292993583688 25786 226285.06 DEV 709.474595733579 1281.35133950876 1150.66018727249 1786.46942818895 1310.86500762062 1686.73908546989 27404.7607244878 9536.37111939394 32147.0049720647 55494.6382019752 28092.9397631172 8405.96006751478 3724.2694651029 1680.97842672911 2002.34656919952 1327.36934115837 1541.80060175494 2868.31592320301 2045.64576737813 1693.31845772161 2955.24202249436 829.224833920737 477.518284320644 1005.18728540577 417.248422019794 1762.01129753502 1680.67974548731 367.133465640884 77.7674615564755 0 8.80042709064314 230.876027339349 8752.27982850183 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-50_preproc.map (2812767 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 4560.38334968854 4839.82275036523 7928.8036747238 6578.19655350444 13039.1298794906 10679.3932033483 14809.305709199 25276.8665930712 31655.2718306949 734310.462157769 23033.0073633484 34954.6343633484 15090.5950535645 17574.5215268345 15088.1446619046 45923.603110722 12058.0568630709 20739.4252808058 7040.80701709245 13446.1781187858 10322.7140100292 8389.78156462149 9919.82797048369 3863.7996005403 3853.40892085007 18192.2605815657 1705.795529431 1470.46352818362 430.173389564515 0 66.8015389395802 29112 219438 2 4329.70210472758 4176.89575261608 8194.94861170494 6578.36469916525 11845.3249251738 13163.9453014451 13590.0089246724 22147.3382040898 32571.6497084206 677885.09134454 19960.3372268942 33452.8256731407 12232.4384367624 21846.7465581619 15121.3607165447 28404.0086071155 13795.4821575517 24985.4157530444 6434.69591902674 12101.0380809469 9280.23500487441 8671.07817701831 8028.61190595801 6491.54149891707 3989.75289589268 15991.3469928902 2342.3609716363 1357.90091263407 532.78305672743 0 74.8691392627583 28936 217365 3 4942.88063065965 4132.68535645644 8156.60449729725 6741.86475220255 12679.1078667316 11107.7259418269 15315.1264026189 20825.2282768393 36369.9420268605 713349.582367812 28864.0363251486 29318.7955682131 12931.7026439879 18475.6663379604 17721.0284475631 29161.3365438654 13250.4014964689 25786.7967921864 7357.31285568729 12940.4807165975 9958.55361487468 8204.99500696813 8374.73558607038 5716.85610150347 3093.29277364029 15880.2255188423 2008.16281399148 1242.38056186419 525.590625460029 0 75.9631518041426 29801 228737 4 4356.06699729191 4789.71776868979 7856.62053694697 6302.39336564385 11829.0080491659 15875.5065870477 14793.2173200592 27607.7231037789 33007.330192993 728016.101304724 20573.4513765794 36669.8921404045 13528.4606160771 23651.9361317683 12821.573999445 37901.4206352916 13638.3522565349 24186.3964773876 9569.10514651271 18243.4681743171 9508.02796383414 6826.5409334849 10112.5400032719 4878.26872923844 6232.38578242571 16581.478126387 1854.70508002532 1896.63399971954 401.223331234291 0 70.5086905010674 29062 222746 5 4925.77688541585 4732.29693101737 8333.7255458699 5545.19785674432 10680.6132090266 17660.7259253577 12240.9178812416 24143.7530788924 24787.598168247 805152.738532874 27903.9758557016 32629.4640890395 17092.5573654331 24131.6736147374 12956.4355953001 28785.6931340312 13774.5981371761 28085.4849585423 8569.5490507007 11480.9414108508 11133.2369316033 8172.27807725078 12335.5695398923 5865.63500401446 2763.43342180249 15490.6796445464 2273.93802144212 1534.63030677264 593.925222953137 0 89.3178510480203 28555 205277 6 4883.65665662015 5184.52686216838 7031.12848881488 4875.67005142135 10805.8670665236 13074.1524879457 13984.2737257831 24422.7327681335 23139.7939486546 706562.154103271 30102.2613486441 28252.6784484621 13785.0060086447 19333.1009064611 20679.6407164955 19455.3003953171 11545.2108611348 27406.5036329896 7314.46561536879 11023.0565232462 9526.90262678964 6928.96399117629 9654.30411056076 3955.57862923575 2715.06817722727 16176.6569971374 1721.48130658872 1158.00920326586 302.712013416071 0 85.1147788676934 29098 226484 7 5430.52649320744 4198.94258724215 9515.35256499632 6493.47228161485 12085.4223526683 11388.9195618017 14123.0590394363 20308.4037653843 24878.2204652133 853934.490209049 19993.3777930315 29493.6314220726 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3868.48731428814 5612.04782994023 8350.24007276576 7096.09154537322 13973.3463439832 16250.6022250039 12179.3699019049 25495.4936932624 22681.1242327768 671081.065827983 27887.8998840719 40526.3081910522 12278.4095874841 19783.4525413161 15258.5662216107 18280.8073427002 14596.74424636 23958.7664481918 9637.26057669757 11242.6307334552 8370.02426871087 6417.22834914643 8949.98341345748 5115.06260157621 2953.26539698283 13483.8915924388 1742.35701648179 1246.73750738393 216.661585096647 0 74.9480535987776 28720 230194 73 5403.84650597601 4510.55903550474 8986.14823334637 4752.4406596577 11819.3137224072 14660.5240389576 13472.237517357 21993.9447569279 22575.3781348844 764658.456015793 19331.2782611578 37116.2736828308 17312.9554595495 23877.1149617452 14113.7596170967 27981.300127745 14542.2574504728 25380.0839167352 7762.58959053629 14142.9636621987 9967.98173731197 8175.98891762615 11493.0241698453 3470.25127430016 3129.95609301039 15474.5278138995 2135.03477822505 912.190066751579 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14807.1577929365 2128.37673210163 1353.10035758724 547.462929918322 0 64.5413084195083 28966 213620 76 5334.47560569964 4905.25912149227 9699.18771005902 6788.98709681486 12472.4020862429 13902.1245867272 13338.5047783204 19614.5456431709 26224.6146818193 746427.894748183 38411.1543374306 30245.9250708014 11715.1722728966 21687.7557677643 18025.0089789287 37544.0535563504 13144.6728804143 23467.8405734621 8224.87613097136 12001.44533568 10055.9021184634 8057.01128745967 10364.1980902027 4418.28558769314 2676.56525056563 14930.1204411752 2076.83305571942 1477.36075088197 410.050342562627 0 74.0981279958009 29425 224151 77 3996.32995841298 4557.59102591599 7233.49790771746 5265.65400711081 11612.201391546 13067.5998516307 13621.5974376009 24157.9198129621 35904.7886586391 801936.268315275 29867.3356347934 29237.6942725964 14834.4030843381 19381.7745291654 17272.2463763782 18305.3185812994 14554.5462412336 20897.7192391974 7722.67904810267 21600.1095480618 9288.13201525243 8836.63384737761 12762.3011511645 5667.67735766254 2718.28135354769 14591.2680001444 2205.98187628856 1408.87871806308 257.822951225505 0 89.230327673814 28974 200230 78 5035.58431868761 5019.36385586684 8620.30852384402 7771.49941985854 13876.949572325 14038.6349475344 15177.7672514564 22915.5757985398 23076.6506999775 672934.138042622 20394.1374478714 34567.454263232 13655.8576344463 18482.8733286189 15002.2101934529 18275.2292275824 12465.1772510793 20945.4697810445 9716.17772808536 12880.4957725719 9975.30717680945 7808.84353028304 7936.61261970446 5155.02661255636 2700.9091762745 16263.0617956888 2414.32412904727 1172.94423063957 408.22517573051 0 93.9752867758187 28729 207060 79 3977.3328917632 4584.49873758726 8590.18632244584 5359.18631770733 11614.0092711465 13078.7668178967 14009.3949151254 24949.1084037687 42010.9674226914 640415.19956208 21117.8704889141 34777.0192095095 12363.6577645986 18524.8289437644 16963.6075838728 27467.1447093919 13939.8759042884 26313.7414561829 7834.37157729069 14093.2342473441 9158.8969425239 8317.92473706873 8032.74404724434 3992.68488335348 4657.48425405357 17394.8952292737 1895.69943160414 1585.41278021297 398.819739848782 0 61.9130195339615 29196 227172 80 4808.1524514411 4466.3601951676 8230.93153017742 8282.89136005243 12139.4496524825 15233.4005019881 12732.9851813255 21060.9547214893 25460.9863403663 779453.148690826 19133.5175840602 31103.4156168224 15912.919068574 18533.3187372945 16092.911094656 38103.2018879617 11423.5706150544 24794.5434998724 8178.104843011 11696.2176118502 9849.91662910991 6653.51321647794 11489.5560090311 3954.48404488783 4287.29398601992 17197.7946187707 1867.09928670172 1190.41362646111 583.140153079297 0 87.9876705710611 29142 229007 81 4671.52628135712 4649.10319237946 6842.19921164364 5781.01793472458 11144.0260840255 13618.535017854 12431.298948312 25164.4352118832 23158.3322129129 754241.915896588 28039.3260840548 29051.0634230616 10895.7080927347 17572.9171617892 21134.8152831502 27388.9840379965 11556.898638658 23261.6787952281 7496.81818331385 11879.331415642 9441.73060482431 7002.17993307439 9977.76814490267 6919.31794993026 2449.05890151301 14644.5649255676 2027.86026727996 1220.24297731467 634.819365627592 0 89.9709984334553 28485 190160 82 4961.93210550614 6321.85072595322 9503.02474752593 6893.50983661179 13041.5831018635 14313.5830483645 15037.6968560108 23324.6580778884 25147.5098133373 740682.111982262 19236.5461305343 33962.515899529 12211.6982690698 18413.8393592803 15349.4186189229 19376.342573716 15102.6272867016 24957.5125148643 8073.26755498923 10833.6175886335 10891.8376716632 7189.54093102825 10441.1701901312 6353.52542466609 4744.85967487293 16052.6170516549 2069.73649124969 1603.06864348235 339.089981896788 0 98.8242905467331 28909 220349 83 5890.79776938599 5710.01752985827 8765.48607624894 7530.65561797802 15378.2574950649 13087.9968333708 14149.3276935924 22846.7152130869 25873.3187598522 777810.99003162 18165.6160902837 37236.7254516748 16814.421776095 20663.5577636354 13303.9098186967 28273.8696526677 14664.8259536098 21176.1980133388 8758.53351168021 13260.7437010438 9831.4480130635 8235.61112595099 11048.6635251396 7724.48927608034 2500.80215523614 15505.5191769945 2687.22821152255 1308.64152394937 406.30360591548 0 95.3992397635236 29058 212880 84 3807.5927900426 5389.71931780548 9244.8394908461 5283.25020958753 13618.742532986 17638.4845700475 14825.9266558144 30118.5563657279 34181.57954471 812129.677034823 21910.0304797787 35124.7922060863 14235.4236903524 24427.3379152175 17405.5222619249 10457.6712433165 13581.6019542358 24895.2151758444 8793.95622181719 9926.64648641716 10322.9182732216 7973.03065913146 11980.1001894925 6202.52428813928 3384.24946024005 16217.5642053618 2420.97387424486 1065.78378045533 369.294315603605 0 85.7233618764923 28925 226512 85 4790.8123280917 4158.14015662012 7480.57026436147 5713.52613301587 10943.6360172782 14657.4668336254 12493.5442706353 23462.3614594543 26140.3148759892 762445.167134895 37367.6815699626 33263.5598554548 14150.2328717776 18764.6204053008 14909.6768886076 9530.27852800906 12888.6377645733 21561.1856477305 7965.27723125575 13605.8731133796 9304.32498356958 7210.10235243309 10738.2683156736 4234.5025599838 2552.36023073114 15544.4495757358 1912.24612946645 1298.81713673972 494.870758613917 0 82.5262429340784 28943 215509 86 4903.80553277934 4767.05884778437 8163.96267572869 4555.41075742241 13536.0208440741 14251.4701295118 13787.1467400677 23049.2081158953 34171.4841200342 788057.512970466 39834.0588873654 29185.6686188007 15939.8600934973 18886.914435324 14291.4195489976 9873.09624214803 12332.8480699217 25007.2304048758 7605.65808541849 16495.1436818163 9390.87084204946 9373.84680446962 11753.3893890479 6093.39424212998 4128.8605615528 14474.5296339681 2049.79700750143 1608.38973070567 389.019120824179 0 79.5222773937134 29102 207891 87 4757.36020492741 4058.6681809213 8472.9031905425 5314.87141613008 10582.0950139053 13887.1541483641 13041.3514019695 21369.2233871355 23811.8144172286 812401.842194212 28508.3209184154 30361.8657303678 14314.4832986808 21209.4825087212 17991.8347441305 9846.18166864796 13387.834845678 26088.1601918739 7636.52646020046 11856.2216866643 8865.45548330938 6951.05505630917 11692.6281649387 5340.26096602022 2631.29468173333 16601.2281786537 1888.98828204554 1450.66757794168 232.108883165982 0 100.90223087311 29189 218774 88 4527.5117182867 4973.18050523991 9580.19489412926 5786.12874489359 14603.2694732929 17542.9134708015 15940.110319342 22921.3854449574 35724.1082059041 822211.861823218 48592.1711589351 29837.2461975694 11882.9972535408 18092.2158688692 15585.3117239532 17671.932200581 14192.6086384065 27574.4011361628 7196.34839114737 10173.7386420947 10226.9597254059 6836.74428437867 12826.5400974934 4736.09088244485 2993.02433639137 15447.8428299213 1946.89866702629 1477.84219511831 543.186825198 0 72.7571937652268 29246 220857 89 4983.45052071357 3563.35073167858 8718.06365579165 5137.22219210729 10814.2329295613 14470.4284231475 12035.0666804276 24322.7868309819 41423.299871313 738260.873910811 28307.741100066 31645.6815400243 14207.3874559721 21398.2507802617 17303.3281801028 10382.1691287087 11429.0868593203 24873.7320700582 7815.44142429983 13255.0810756269 7859.83067844567 7209.6715105873 10855.0380403565 5600.33499828807 2790.64868634242 17450.203857265 2361.3215914197 1615.48207531134 150.564602293332 0 73.3146502310564 29093 217965 90 5031.83520864466 4169.96056685575 8394.77418575818 7385.12643908996 13818.6380837395 17431.1222833345 14824.8199364114 26492.3671836864 23162.5414452216 742798.347160918 21365.647940104 30611.3228282141 13765.0784174288 18318.2665889866 11711.1117023623 27772.0407745661 11687.3330811566 22995.7245064985 7164.91966167087 11894.7791936053 8269.44111691398 6516.15217416039 10154.4125517536 4655.48446166855 4127.74154085263 15649.5400029592 1892.39645735394 1399.61927983406 445.546132171891 0 94.1984449553347 29237 222514 91 4517.11504333207 4377.60489867655 9203.80035707187 6658.95457877364 12417.9639176394 13770.0023116839 14174.1618561082 22997.5976501052 22090.5643696343 701508.790726115 20622.1916635452 34356.4691280707 11797.742993446 22432.2802253262 12522.8307909594 9257.25570764082 11831.5660817164 22145.6481594088 7027.31887196923 12448.1974254295 9540.85539198734 7071.69445543976 8619.0230241479 3509.11478256301 3565.75113497727 13637.4432568244 1833.6948835161 1147.77605066923 290.251732287342 0 90.9009204811331 28980 231956 92 4690.64097032822 4726.91639317097 7780.09211580472 5138.43600254843 13676.0147128125 12884.8711795187 14309.6733285297 24608.1888078739 32647.6763717272 766155.875213023 19447.4489749218 33505.4538390953 12644.3652739024 24587.0851032558 17469.6676171417 20307.7393800883 12376.2176959555 22773.9098439269 7462.42175591796 10983.7167771252 10575.3815472079 9564.30121215702 11595.8259060013 4617.35817254917 2927.57852278848 14379.9126315346 2307.14220630327 1094.66411924716 235.122081000986 0 87.6963594486342 28799 212034 93 4496.08002347237 4867.95870103621 8795.35177136437 5267.1892967685 9976.1935267816 12850.2174295329 14320.3717554275 19818.9930919453 32243.2360243917 765243.964452346 19961.5459589152 34569.2874974714 11540.5969783608 21270.0026126074 17601.4731868326 18270.7321492275 12051.6767111559 23228.3568446303 7461.36387742858 12447.6150944056 9694.61547169216 7604.63904662962 11738.4245945145 4298.63464685631 2720.40765742193 14722.4809614678 1944.42366639996 1324.29809491797 452.140647484207 0 90.2724192690799 28735 208153 94 4700.61317314351 5396.75410524934 8514.06668584609 6687.21766154595 13545.9021161028 12443.7873048882 15058.410506948 22894.4905006268 24558.5771581297 781421.01909194 38494.7474578899 32720.6670299467 10693.6264886583 21218.6002860319 14012.8440764272 10222.865672427 13953.0676553007 22615.6342404946 7190.32904507025 13887.3895843199 10213.9718427648 7257.05887390894 10473.6379121402 4642.81092821248 3165.04409739115 13035.8190076827 1822.60787720362 1571.1808704239 237.138982441121 0 85.3232834631146 29109 213563 95 4222.97652481227 4335.51687137952 7702.62618896395 4759.02516565835 12264.7779595315 16474.9650878471 12615.7081279491 20336.2037912585 24092.7994524008 769707.24990323 19372.7984405787 30106.9670634597 13976.3248951503 14803.4591066861 13389.7478367231 17837.2587439653 12133.7112129495 22450.8628710602 8210.23226710258 14413.442542418 9929.29670986039 7937.74605540036 12246.1445706609 4765.21311807442 4025.15137673313 15178.8566063278 2057.77825639696 1649.26147923358 262.471408798559 0 111.187650413473 28674 205729 96 3981.5971432668 4949.14702565289 9343.23976468357 5340.43313533726 11267.9140946475 14611.6771299717 14700.4450747045 24384.3279603219 24506.130681232 733032.590740394 36640.6034326638 37483.2319130317 12320.8745879492 20725.0242662851 15908.1696241592 18534.159905632 13088.9280986024 24622.7284296563 7120.35196785146 14617.9953908147 9208.37122323158 7028.45277124256 10352.2133805488 4722.29293994577 3306.56881822042 15421.2922194097 2454.47218865411 1860.87572496069 393.503275698778 0 76.1701120511794 29157 220531 97 5460.06805368979 4890.30553233751 10187.2736478672 7116.67162486296 13837.8504061774 17341.4801404659 13752.2742233541 25762.1954584428 23473.5472810927 832440.104886984 39485.2339310848 36433.0617724106 13829.3772957454 21097.84707815 17366.5342777053 46191.5958571387 13415.2557135919 19999.496211264 7423.50243237936 12809.3883927422 8426.06169151469 7652.44174531514 12516.1801854711 4598.73988297557 3003.4110487534 18228.0071250559 2147.79064660272 1490.61330196643 782.742771359226 0 72.1476014394868 28745 220872 98 5272.67323668741 5810.81104959426 8582.79843320756 6682.89253229156 11348.6086283247 15512.2838539731 14813.6341194377 24779.8169151543 23679.1919483883 780092.524315736 19530.4726605408 34902.7974256374 13669.2063029444 18815.4605977678 16983.9958139712 9211.73653745912 12633.6971123532 24214.5999691836 8759.27439603545 12984.3668033447 9194.02934006295 7934.07440985947 12916.2434200029 3858.40711591963 2824.77676027209 13169.9595172839 1849.49014615234 1373.90881661794 356.964093391662 0 101.578870297572 28862 211282 99 4513.39094786726 5688.73675712574 8187.05118380141 6707.26179767369 14562.3017725223 14907.774173957 15520.1511957897 23901.3757419631 22721.7908037825 752134.459128546 20805.5725276803 32062.5056701939 12528.5694668127 18892.297866363 13618.4509462043 26592.3990150801 13943.1067273359 21094.6572889844 8662.84329726486 13594.832690851 9311.70116361942 6196.47803692811 10458.9370150552 3890.86696619318 3796.20885225195 13681.8543763625 1862.16654340202 1256.34168148711 508.10390909861 0 101.288111694219 29283 229348 100 5288.9029237506 4576.70994089161 9060.64395703379 7213.13521158653 13779.6243415596 10922.0323263347 15421.9658799226 21616.165862557 32715.858850332 888793.986367762 39696.7751853739 31334.549726654 15045.012232116 16737.0573626676 16885.635098133 17217.0624529156 14388.2718783404 18925.5490787587 6821.71905058308 14144.8163246178 8337.80301816691 7726.25201874515 13705.2818613921 4299.41519585532 2960.20756051708 13988.25603082 2113.44982396292 1363.24040356975 223.729770385792 0 94.8100609616023 28824 208389 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 4758.7714456453 4761.61393901435 8362.85682322858 5804.29772310928 12366.5408463392 14305.5475262267 13780.233263408 23543.9490876291 28356.4972213287 772910.309716773 27765.0875929036 33578.4468674109 13310.4210342356 20122.4155190669 15868.3254586408 20685.6164487767 12983.4180809012 23491.1830841312 7751.24105452436 13088.5761623781 9517.12132933122 7657.84242729147 11090.0084244218 5005.98841046995 3323.03607309781 15299.7950833371 2124.42728229467 1360.7504154321 392.750105669487 0 86.4915788242977 29015.7 216470.33 DEV 458.776541061264 469.514806581003 1021.4082249039 972.578822059305 1086.23199763955 1941.75363896773 1073.42029506686 2334.86181121214 6108.99615821245 46295.2856960193 9386.56376217939 2736.78954184069 1758.2941062406 2399.50002349604 2107.96612871696 10662.2170911674 1229.35145636797 2012.2556034068 689.455694654886 2161.13842305434 774.157248654841 775.557461210112 1290.04768280955 884.895034827193 726.830652997377 1234.86563385147 281.05729379188 198.104198391465 149.770433058149 0 12.7249861398412 281.654948474192 9194.75798491184 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-53_preproc.map (3496970 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 6427.27167031914 12584.0499271148 9079.67626886733 12111.7003970945 9635.68398647988 11071.5027817596 15495.9748339401 17463.2750574299 29852.1982298458 543838.181005462 28017.1025351073 17646.1138186121 23512.2866872147 15749.0112073044 33088.4489929754 279472.059908756 14015.3776851126 47474.04169372 31420.1185820826 26371.6864936143 30915.6271904 8984.92349120567 8417.05291562117 10037.0055004437 2697.22349865226 6079.71085990838 6123.23975504092 2294.4558902426 152.306689901604 0 8.67380699143166 30519 195111 2 4746.17354070375 12152.4481271176 8874.58997918312 12434.8500935725 11316.434195795 11922.6295485622 14148.852678449 20528.649574355 23075.855771908 587576.670972081 25931.5037814976 16567.4185275923 30030.9197919645 14964.0465820307 30031.9363482515 276685.716285119 14444.0481271129 45772.2343794975 31246.969000171 21723.6742242401 31560.9848022528 10208.6867750895 8377.13430712797 7836.14435963417 2948.80956249899 7577.97145641298 6256.07021899152 1753.05781780782 131.086552904837 0 9.5227048105326 30263 180901 3 5171.48717650765 12908.3521829282 8435.21333055992 13752.832515593 10407.7731677547 10835.7514967212 13345.6665897924 16839.0604443383 25452.6723033311 498414.406554889 23387.3441606734 15200.0193892081 30919.8797939411 13627.1402789036 24962.0053360191 277326.403472754 14609.7171819737 45192.7954697321 35880.0112935818 28155.4457843194 31979.9211500918 8731.56181093527 9102.67310690071 8962.50554612056 3376.49967216334 7050.74611686126 5759.02489682642 1978.8647366408 206.247102159034 0 7.96228548399858 30493 187218 4 4964.80546392996 11565.2028168413 8093.90071215205 11344.0510405161 10258.915679186 12218.9446990179 14852.6156806818 16970.4431990054 24910.672562196 499712.954947481 28562.6628035956 16857.8009181235 30969.0098918046 15173.3929343481 31613.9479653506 305047.021287359 13810.6096333767 42679.129281689 34337.754358104 21561.8837500714 30770.4546344578 8588.68958145958 10913.501214524 8660.52815824509 3159.93841928718 5618.56183636137 6700.77934385517 1616.20559976627 142.54402836244 0 7.20984489262894 29700 179973 5 4946.12168490185 12816.8594582035 7445.78738415234 12685.5779320962 11240.1436347559 11778.3567014085 14538.8620930232 20919.936956313 38491.0804559957 562201.436612078 31432.9597832033 16910.0375502495 27348.749094626 16805.3596712051 31133.823210081 268929.146020837 12168.1659046783 47727.7714899411 39788.4119927543 27441.5177778658 36009.5033642653 9336.61096114063 7600.98041419993 7701.45020592064 3336.9242032603 9190.37923042944 8038.49708398314 1422.81834901761 162.687056911875 0 9.34400399026249 30007 184575 6 4295.05358981672 11124.6386000376 6098.74679729771 11170.3137344813 10766.5684518063 10482.1980775626 13351.0873507564 15661.438660539 30061.426159301 569901.517345088 26606.3811727389 17533.7553045498 24010.264971462 14266.0300192918 29843.5082355726 313706.27845629 16791.2722007258 50824.2138321197 26141.9028073043 25404.1313801225 32515.5644957331 8625.21319176479 7571.82540061564 7900.40109801371 3084.81299815572 9303.58510686495 8682.65022432239 1543.29876124973 153.205885473947 0 8.03336634440583 29989 187448 7 4687.73671566507 11890.8808929295 8319.07243553316 11646.5636632821 10289.9086263321 11366.6973915839 14071.8833018077 14928.0846855428 25925.5753004936 568770.31917149 26406.5599202749 18245.7649918645 26741.8794264164 16510.8093047015 26643.3648081393 291435.547678249 14443.7087150851 48947.9570845494 32643.4578266505 22478.5316497337 33382.8196437694 7890.86969112515 6818.27129929535 8814.03951486189 3305.13373343362 9656.73981391575 7844.26044860814 1359.90531575852 121.920987318712 0 8.68611746079204 29975 184752 8 5485.84420326781 13207.6307151445 10101.2905751559 13935.7685174942 10258.4314704257 12738.1845605882 13728.5551547342 18194.2056013344 29901.0260559672 574110.158118535 27700.537813702 19456.5342130843 28367.2612965368 14159.5747154979 27064.7291619497 291623.170367841 13450.8518825759 49747.5204408607 34090.7035674807 24310.6785368755 32298.5725886625 9131.14751501128 8435.66902406611 8923.60417240777 3203.38519949043 8019.60289134706 6213.31993255176 2073.71071077354 158.742587451674 0 8.77845760351237 30066 178516 9 4322.97613221357 11528.5898084198 7821.68414138455 11428.1183632666 12034.2490332466 12639.8927289304 16068.4285041218 20043.6951270352 26020.9583360906 568292.325691151 29012.6218378324 18744.1995289457 31775.7934066011 16129.6829015468 25056.7348795925 298102.037689826 18675.3678349364 48922.4025583258 30773.8597520427 23162.2419458681 33608.4326369654 9387.04245394942 10513.3570965607 9104.37001208544 3070.77957815026 6551.87561487925 8363.18369990308 2688.05151306574 136.474783321908 0 8.48574855534777 30029 187685 10 4557.89683990275 11585.4041101475 8648.32952424122 11574.7604906446 9870.38631953266 11848.6322728267 13726.6809813118 16805.2710858969 23959.9776910348 492432.962117181 23490.9312862732 18900.9804513782 30131.8075083154 14277.2111399139 28286.340825691 299976.52558592 19171.2195309182 44919.8694097001 37106.3816648041 25250.3877982169 36466.036562746 9348.10371133606 8479.24454241806 7837.87403160701 3341.00767906452 8236.24989361685 9183.87782371764 1828.9202577826 138.369487345218 0 7.22579066086302 29161 187178 11 5397.57636062894 10602.5012601817 9054.64286619042 14756.2545835035 10543.965331142 13056.7874600037 14611.6172064075 20817.3365685973 24452.7523123531 554215.580341943 24924.537771461 16344.0880424268 27679.4911701303 16857.2206668507 27623.9332976254 301948.673318718 10282.3240511304 49269.1125428452 31818.1947294876 22228.0036033129 38725.8334147443 8482.66819718267 8243.32671257341 10010.4697554096 3375.79962084613 7589.92376640246 10172.3832488995 1305.68444723381 204.448820384287 0 8.1407116730795 30561 193176 12 4404.00952275325 11060.3973848606 9766.39912862976 11713.1028816129 11398.2413069122 11189.7084094873 13052.2461322902 15151.06379333 22224.4908045991 521078.595681921 27821.9623233009 16043.650912123 25197.3129911256 16002.4779726713 21184.598348127 279994.126561108 10693.4572473468 42606.7062189038 24222.8112401325 21316.0472628471 28534.7366130907 8381.86020652263 9775.80120751817 9027.15696707533 2484.83179357871 8450.27833305836 7028.07141470903 2578.78499666131 145.547298480937 0 8.2706049652413 29714 185561 13 4641.38661616662 11838.6325406085 10059.4396864145 11403.5324843291 9899.60005901716 12411.3490421361 14678.3939159143 15924.0809734201 23584.3719953941 475398.578823056 30000.2544773874 18602.3149160385 27856.9104743628 15095.5739931238 27874.9614773327 278138.307508165 15663.926516421 42047.8832748963 29204.1489307446 21362.7135702527 33748.6714153296 9197.76547356958 8455.0880960504 8611.44522591587 3242.91727057698 6044.91943770461 9869.75716622434 1963.91613053319 207.871962393589 0 7.56215227313729 30030 181713 14 5484.67763590888 13158.5345434025 8857.13249257036 11615.8235478992 9164.49199567584 11223.8657249175 13675.2377203178 21598.3931052215 23157.1516024366 534851.533188117 25790.429408164 17368.8075751517 21931.2011208487 14200.6132114649 25690.1272778332 318238.948439449 12416.8859295373 45139.6317424858 33539.3582017611 24702.3886248196 35413.5549342624 7780.1964323529 8891.73483847915 7392.43063643 3766.98668994798 5800.48750493864 6541.13322567049 1090.57338756675 268.336315705608 0 7.40956748741769 29756 189108 15 4796.13100281114 12532.7757486448 9262.40964823443 15276.2425243147 10645.6334689565 11685.9984316329 13961.1761916523 14501.2647572375 27103.9673415576 478772.105954333 27594.5952511175 16678.256442763 27685.926966359 15269.1963926526 26917.0416279398 302015.477717456 13704.0601411214 53879.417114906 29519.0331177568 21086.8773959376 30020.6016305474 9340.1292084989 7999.25003765751 8037.37070872457 2850.10520512737 5420.44993299207 7943.17832679599 1734.31267762418 125.947858440199 0 6.94603214099949 29739 177622 16 5083.68286707269 13792.3781049693 10476.089475807 12442.5511434165 10055.7852222088 11776.5698616426 14372.7451564013 18036.1891241833 24457.9934842723 536996.331003368 27861.1720885638 17603.1680842935 24656.8327503195 16520.6667500318 32204.1239660234 258127.115713703 13317.6473008752 38017.8819727343 35946.719432885 25262.267153326 35180.3473588983 9587.59908976813 7139.94105340022 8259.18859338274 2689.56717173169 5782.66393703394 4334.67943303726 1498.95944086239 132.240078641431 0 9.34574589926006 29852 174682 17 5772.40450090188 11908.8651845907 8527.16863325416 13111.4871020605 10423.6639248114 10427.5784557471 16377.9887229369 18819.533007803 24481.2994186506 566356.326924707 24961.7874600829 16822.3918924895 26423.9484528443 14320.3239202041 23927.5472842841 311455.893458032 14367.2482263119 46244.9439258912 36325.3998651272 21749.8140577956 39093.7345691208 8474.31919773553 9673.41938545788 7152.6828849346 3246.93657558714 7748.41012743348 11116.4833383448 2061.48824191801 128.367407516323 0 8.06064883254953 30102 193348 18 5143.98517951144 12446.0367997995 10009.3809152959 11185.6353227624 9563.8363497058 10202.0195012252 12663.5194286116 16087.1345013949 25161.9308982506 529215.676742026 28147.0957831545 19125.8567828147 28690.8344793237 14820.4238789976 24615.1779234651 287000.674653787 13942.729553616 44707.8295962396 35637.3025140688 24354.5251766826 32430.6127698 9381.34017336352 7923.60906296259 7890.32120131608 4582.49576670456 7643.08694979708 5984.17280878965 1473.68510048068 208.981783097441 0 8.18333966469303 30111 185412 19 4815.0656319987 13318.3930790769 8935.37865582898 11438.4682974721 10075.2082377166 12063.63329711 15224.0959815684 19857.6908092072 32620.7794305417 563748.681679384 27681.8997127929 18206.7855828129 23822.673299486 17165.7568103902 26439.9835464591 285111.431469161 14208.8027131324 44939.3964350511 34218.4959463811 26074.0237546851 28910.8125208138 9237.56244947927 9071.84027467566 8642.19940136998 2911.85602914517 5656.45936707413 6815.30533683704 2083.19727374541 133.392902744449 0 8.82963539470806 30434 186687 20 4857.58955400951 14997.0034776193 7790.83672277942 13601.3997801256 9337.41163539256 12014.2326088469 13083.8501651264 16680.551240965 26507.8456953615 527137.099243815 27619.8815659906 19018.9444621927 25758.5831505155 14747.618660849 25788.6360636666 344866.658477328 13516.7940707104 42734.023733626 29363.8259468557 24388.017903241 34894.1253320428 8300.87030891231 7219.65524480367 8639.73591149452 3114.88572507964 8860.88837366673 6977.72508276552 1448.67569025513 133.374507467221 0 6.69695492658328 30262 189757 21 4812.07922036625 13244.9584983229 9592.89504223421 12374.5894504452 11197.0066297994 11352.17712796 14258.4735849989 16848.0364453814 24328.0947011755 552961.896610187 23464.3867913112 17011.3845232271 23067.4006088048 14897.8962580017 31911.7348479908 277117.064031595 13278.0171000671 49315.5987392084 32267.5069693184 18795.9582700796 34309.3612940607 9545.25135780423 9642.78440662467 8417.44454264412 3557.39979982897 9255.13078048951 6158.28640572456 1898.75420994838 111.618481976061 0 8.89612571593086 30265 185760 22 4875.85179580884 11771.2069207059 9499.41075306593 11646.0028768258 11407.141116089 11626.5474732174 14884.6176519957 16329.2463274696 30565.2484998606 518613.818409837 28589.555569219 17895.8309134941 35376.7594562983 17272.854909066 26571.1829932299 277038.76314002 12646.6837828681 41651.2162032896 41393.5395680247 29025.9482256051 33743.4875493876 8781.01239466192 7267.19861274239 8218.12793742039 3596.80090733885 7807.72523875992 7058.97496736339 1971.16946902456 177.240485371106 0 8.31839399392721 30173 186589 23 4593.20281929579 12877.6041189965 7329.17300722978 11921.3037737357 11379.7910212805 13347.5551968847 14898.3557987266 19939.5513005719 21695.3653755971 578141.574686522 25070.5630123116 18209.1412342122 27992.8073422114 14261.9908810571 24590.9871238317 323042.405615102 15393.4974780507 54719.8421671467 29869.8715730763 23884.3206842276 35331.1566786475 9513.06619466072 8265.95382164172 9213.1952365877 3382.80391460198 10932.1806514672 5078.7543908536 1787.2689049783 124.540478359619 0 7.91035948632493 29867 183983 24 4941.07515969254 11511.4310895538 8077.30115029023 11144.812667316 9975.37869164692 11907.2878916982 14598.2104618539 17201.0328251042 22535.8630274949 538634.280240929 29679.6780872726 17455.9428254425 27106.4442481348 18240.1765159404 24239.5772060194 297089.464222406 14222.7403943341 42621.126367161 32930.917677258 23444.3826638714 33629.5042749231 8133.715101975 9468.2518816869 7941.7298563366 3370.50775964602 6223.29720979192 6332.13269753335 1409.75609683229 193.20990679797 0 8.04463032932752 29976 182759 25 4667.89197442673 11817.7836567624 8664.01062538344 11773.3573198899 9079.47238505111 12531.8789270796 13663.2468721923 19185.5599645917 36590.9213088846 533507.284548885 27954.6460611834 15993.1020165506 24298.1557400178 14652.0567496685 27669.9169800901 304150.419703882 16091.2438426625 50884.5354664858 32730.395404906 23550.1361541519 35208.4383967319 9061.74883017513 9438.56007488837 8400.74696408308 3553.87921863606 10795.2919309776 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14282.2435789337 25812.5761001892 313178.893337139 14664.2618673813 46742.3928929132 34247.2104767826 21465.5370424574 32317.1925636892 8491.69739078676 9187.05591100743 8255.55394943551 2800.30832750057 7233.94305429332 6228.96804893995 1441.95790005183 118.623221243913 0 7.72403704338469 29650 173114 91 5981.14630949336 16573.3636676408 9393.97284789094 15210.7105213389 9536.87519411182 11615.1187966429 13373.0753515348 14752.1644064178 25493.722772128 540270.568190312 27751.3559132609 18143.8165284619 26939.0508066267 16114.036832937 25933.7973095225 292772.159530882 13404.0151637516 41690.2974910063 32772.0535301953 22401.2390002158 36071.9920408846 9692.17998107401 7760.22609001295 7712.28573318733 3035.98532618978 7513.6024795727 7412.04161460385 1740.90389584119 111.972592366839 0 8.21117714800354 30265 184100 92 5041.61567349763 11336.4783654249 10352.2200372937 11474.3198933515 11207.4310158412 11245.5593663501 12398.4530623942 16212.0330765507 23578.001303665 603670.975092512 27572.7610403833 15970.3954088877 29222.1899099102 13742.0391941645 30446.4641517386 283058.671515991 13292.3234164237 44804.518114228 30453.6784274592 19158.6674260513 30621.2429867654 7811.22915563718 8833.12242246822 6295.05237526585 3405.24812640946 11854.0418449339 5832.46783759753 2041.24536152724 198.881824073055 0 9.54937136471606 30162 181102 93 5004.04637774272 12727.8573670472 9033.46329148506 13537.4076180636 10786.7443640828 11725.055955986 14596.7825281723 15929.4226704114 32042.4549010211 502910.504030921 22313.520905852 16231.9029113706 26328.6201466289 12448.6689568094 27936.824364257 302363.958085491 15074.0086731868 42843.8749960841 33088.026586466 27783.6417285959 36275.014241938 8161.96390883143 7560.33808892352 7534.63001649563 2816.32631073736 7159.86950364016 7758.27167497244 2181.22855066997 117.86987246687 0 7.33332885457705 29926 184369 94 4808.9539226843 11347.6420183952 8344.98609113946 13440.853083438 11424.4620435144 11381.9775655013 14826.0646169142 17508.2615648615 26568.9998796077 519406.981609076 28139.7314922334 17930.819501602 28444.5324494063 16133.8855127827 27471.3873644963 302474.002611559 15751.2080016449 50644.5249037468 27972.9164106892 24202.5867000425 30331.8905560618 8422.28669228309 7218.98359037406 6820.06344274728 3244.05654650025 9113.57763327428 7759.57429701635 2090.26430668557 132.377482361554 0 7.58196378100039 29975 183841 95 5186.06896934586 10582.197518815 9716.78939469877 12224.0280225842 10601.6335348997 12502.6744277859 13766.4551391268 14650.5060640357 24364.4958781203 513566.425210223 24610.1473585239 16073.572273713 28795.2887048105 15784.5219114286 26987.4261278595 289076.939034887 12090.8838176161 43146.8622384098 28955.7009065752 22208.8959138755 30788.8397627859 9990.09882938883 7492.87503433804 7444.41682287372 3110.64138171235 5695.17017389209 7945.21007841971 2804.75446283561 232.607124461204 0 7.87148005837201 30321 187911 96 4451.03175628194 13520.5537929041 9968.66767294435 12377.9517338462 9490.86622833342 13159.595924444 16070.2051491378 25828.3572641871 23794.6937178037 572799.237132963 28089.8781395664 16953.3025382289 29901.8767247803 15048.2015066824 28595.7922605906 267765.817737409 15311.6061891759 44432.7754379515 29372.0027174515 21127.280366919 30690.2567825686 7700.37820812931 9413.17513398658 9198.96343002406 3088.70650730629 9166.31508781474 6677.18125665149 1204.50079766379 214.924465186654 0 9.61827599448897 29783 183895 97 5131.87677576206 11123.6514313943 7890.42486925937 11543.8904150556 10066.5496130358 10748.4964964729 13474.976231496 14474.7888284888 26339.7198628264 517899.575820455 24445.4424414588 15321.0427827695 22189.6669247744 12522.1635222124 24941.5761022812 306968.706187815 15589.9462245922 43492.8839181771 34902.1053840105 24692.035475736 32759.0666962558 8920.49187688099 7273.25792158109 7164.34711888097 3121.8854518858 6182.13742785144 5539.2216127337 600.619536820579 178.021381242114 0 7.43485739579579 29701 178632 98 4999.16809947078 11862.4587986781 8575.38254713097 10385.5498475632 10356.0715275977 13692.2765208264 14591.1229483412 16871.6155215354 23755.2118089026 536906.483979103 29237.9693852204 18032.2576327202 29431.8856449705 15430.76467434 24533.1154303765 265092.342096716 13097.0290584817 44685.0560661321 31793.1389363067 22774.964590611 30747.8163998829 9337.04152720196 7567.74475817992 8011.01888513463 3255.54719660022 7604.50460337148 8071.76526501707 1367.28132716455 112.338245544474 0 9.05734584225207 29931 192359 99 5150.27720284236 12251.3467924982 8217.22553406694 10790.5359810292 13889.3686732983 11566.3350157625 12801.8406724009 13918.1085835271 24656.9932398526 587530.770471356 25295.2575443275 14209.3393110239 27542.9763839499 14675.6025012682 28943.3380062265 278251.713118772 12909.5179655085 50860.9274208 26007.6942656675 20526.2838314733 31645.9147300592 10352.214389164 7366.13895322207 8329.40022084118 3062.93234353471 6289.01320351023 8133.25514532389 2113.4558085468 154.010140975707 0 9.4332294044334 29667 179316 100 4617.28729292195 13058.6657068294 10184.9340366503 10702.2399973834 11422.3982809075 13276.8581596417 13505.0110168802 16362.1780497952 36844.0674953315 496056.469718183 23815.5934766796 16720.7010319079 25027.0002754507 15101.4613626862 28585.9336247078 312096.735491877 12246.8001131954 39610.1859044513 27046.4685789587 20563.7536749495 30502.0806221557 8685.45390482399 9444.49387564644 7674.16372294617 3631.60101267723 7657.5848442519 6067.00567468221 1048.03259784324 232.605119798845 0 6.98583382532976 30232 198910 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 5120.88343632904 12507.8924209876 8974.05133158653 12321.4617007787 10375.8239007887 11880.2313507967 14398.1645896683 17599.3570675834 26685.9122369721 545780.511191897 26461.4093623991 17032.0460308288 27088.6514379779 15263.6166136638 28118.7364347235 297828.469256845 14150.1257037479 46303.0635114832 31884.6387719469 23027.9700313209 32989.4368390573 8814.01854300271 8235.55774072515 8155.78202744655 3165.34208138134 7765.99515365956 7157.34874071659 1694.80663364495 154.074387761053 0 8.15599629920941 29944.37 184529.62 DEV 538.361401882498 1335.3217536811 1067.81384927075 1137.97763407943 931.446797188986 907.454540421925 1023.65197419464 2531.18757051513 3910.58128931173 31768.3782341935 2019.68459141751 1130.03350986574 3036.30888135605 1438.02802725598 3147.79564190144 17082.6918919301 1644.63679089647 4089.89087649524 3812.77357265796 2171.60245318195 2511.28871212325 733.708195418166 932.132589765103 967.668959669203 336.881056344937 1536.95149073781 1427.60005210632 442.022824864652 47.8160469591908 0 0.680285034665991 291.847893773452 7070.02910995421 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-POC-57_preproc.map (1585882 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 1990.86312898469 2664.54202747568 2353.73690089292 3417.12742822277 4912.06454959577 5393.25822197246 8527.92178492165 10922.2035996343 29446.7947643042 426475.366349594 53554.0724594504 7956.42573246382 42455.6335313613 16060.9558641959 22967.4673551776 166285.699989279 6204.03365452576 21747.8302950481 9177.24980728385 4534.47737105098 6272.98164829122 2392.75153514843 2990.898981794 2605.25943718081 1493.43032893476 2959.6735609359 6483.11690975351 742.440745041774 373.502930934153 0 11.0117067415951 15179 118521 2 2021.08857367206 3615.09876995457 2033.5255738048 2913.76738381259 2946.77718273984 5119.12382428496 7419.76268643202 8113.02249108234 32415.4821209748 413220.807734367 55115.0258043717 9425.66362060061 40371.0522274451 12894.9108304723 23610.7526134686 157769.279775511 5804.11834199496 21616.3647894794 13118.4766933249 12093.1902783095 6341.1471682766 2831.16761477143 2295.79090486932 2957.70165363679 1603.60372496593 3887.27696296901 8556.37779278017 817.925960698385 89.9475903194305 0 11.1921509307758 14928 126063 3 1617.0850866074 2578.09824889585 2048.41210323252 2875.74060066294 5835.89411319808 5536.93512923986 12293.9743974137 13403.6147766503 24094.8648942844 381893.463339752 64601.382898533 8273.23534329978 33381.6119874596 16160.1594926339 24749.2622708597 191233.325216926 6492.8370708834 20047.6277336731 12300.6599550199 9467.23516483058 6045.89491564857 2134.6884961368 2105.64950441219 2572.02464304244 1793.65887313708 32760.5983836759 8353.34925447375 708.66782205996 192.518958679165 0 8.52319451673726 15048 118066 4 2135.47885736337 3309.86023014591 2031.03789461985 2437.50002273263 3669.70229449026 5452.3657666425 8557.50878319494 9775.0673962228 26737.2607788738 398473.9155004 64483.3291853294 9857.57321389088 42390.5521880978 12823.3123443963 24684.0203991616 197265.273551072 6071.86440359627 21725.4606386417 14545.1223520918 9373.45707509478 7279.67618365863 2384.21150748611 2826.75437693924 3007.32499686704 1663.91230348366 13925.6507430218 10110.9913335277 1105.1924823544 194.401013091782 0 8.588546949953 15227 131428 5 1920.62149015012 3129.77968800925 2308.65286547559 4490.27876515612 5612.05777090126 6056.00120427319 5677.53346249002 12417.9270135818 32490.3933792215 438309.69262494 41822.4984927037 8778.79444214341 58209.9712086816 14799.9473824781 36606.2929177039 225389.308267702 5594.2957759158 16940.59460491 11549.2931144647 8008.94857850227 5812.33547507434 2578.17840961279 2707.07766531684 2908.44368868774 1459.16384796063 12835.919234437 10155.7247666555 577.389041872826 148.078559056562 0 8.35434055522175 14856 116764 6 1668.94892989464 2626.54808426401 2231.45505574329 2745.89662537728 4077.97012241829 6641.84660047657 7317.83382765719 7330.26959209419 17368.7612079376 407207.511264363 55822.7024556412 8686.00295908076 40860.1523798011 12947.7957243098 24876.8614413876 198691.703162669 7500.91652905878 20048.6115903226 8989.99529753165 10874.2866500488 5685.93737562094 2876.34212654526 2297.37084575026 2768.25138868085 1715.26883996568 22001.366797004 10247.2013236175 966.179502955122 304.13952902806 0 8.81827716828851 14973 118888 7 1976.66594257706 2451.92806499714 2113.87578581415 2586.53099983276 5943.3399948616 5092.79609477958 6515.74425965945 13399.5427624352 20697.2745214639 421762.275899404 48363.5388237915 10139.7827698734 20424.0605844621 15274.061856106 21429.5313871208 157274.567424929 6588.00662699539 22238.8019594014 9855.15968845985 13921.7742649016 6457.397472151 2811.87102323226 2621.44015028791 2092.32968468978 1369.58935847702 32547.7822244272 7983.90166472522 976.324848231931 246.53416379212 0 11.7985184163239 14861 116173 8 1897.09133580699 2700.15085150296 1944.63147544549 2968.69580405019 5900.2583429553 5345.72243588136 7683.97508261005 12874.2684828298 16826.15547666 369707.557625838 44443.8484928463 8702.17501346297 12049.2688112214 16044.2447320667 25763.2972357399 190657.400679158 6733.49388132554 23263.773499543 9022.19319950138 7914.21889830798 6325.31786213595 2782.12401645875 2590.65193708854 3079.66421188721 1558.55429659616 22450.4635699845 11045.9501092441 922.479540878969 286.691579418596 0 8.45835128115486 14597 104676 9 2068.99619554026 2894.96322447597 1901.43322569224 3277.13381643875 3664.30732934169 5125.20774271673 6537.10716392373 6968.98883473351 31252.3975363819 423230.314395898 61549.2872651842 9828.7658463232 76915.589687639 13184.4594220115 17722.8663097106 150855.796792759 4994.13013280688 25268.570383172 11510.2892379033 6369.94212049183 6374.99658383144 2246.63495764785 2223.5130842851 3018.27674102877 1638.6569809905 42958.7458246445 8285.85836632827 882.108266234416 282.749039297463 0 11.0842036240981 15168 129226 10 2117.44823607223 2776.61387626749 2182.33687859751 2641.85250093591 3690.03352373261 5737.56105405474 8332.56172063398 8369.4650708364 26324.6155618364 437645.874430854 46776.7362294406 7789.35710766113 22170.2882804232 13966.6888706569 25520.9527815168 167564.161129257 5176.44000874125 21645.2696530987 12812.5328322653 18931.5350682887 7742.18236140457 2632.95434208436 2621.46828056374 2316.61603359666 1507.81119412012 13352.7858669909 8410.00773195991 725.395718690374 256.262890186704 0 11.4844361251596 15369 118013 11 2216.2804603994 3085.78779193126 2063.95690924236 3036.72535933032 4795.02228456272 5642.28723380366 8551.48960163129 12662.1067492658 17851.174465583 381127.510512858 40061.0517026996 9046.51615896824 42070.1564536141 19200.2808040803 21927.5270499796 156709.251814815 6071.08848287929 18903.745020448 14208.0881414008 8284.71060679217 7824.32966370247 3165.67261922749 3191.74512722057 3056.85516305864 1716.5925909115 3836.56144164005 9247.12327344437 649.598698379866 122.695291433712 0 10.6129945436033 15124 117297 12 1754.62640917744 2889.30679993888 1955.49448532942 2851.82729227624 4548.19443923233 5798.17230109616 6692.99477722981 8967.53700514309 21371.0503938819 365789.724133784 54785.2528779827 10285.1658012116 28293.4960814929 13725.0498197143 30086.3165522572 221470.142787699 7200.59131399825 14354.2335709358 12157.8071892969 8754.6306443099 5560.1846511928 3156.96694885513 2561.46647403236 2863.99695352424 1235.46554072625 12994.9897048101 7757.16890177681 584.177369278256 96.4876516675932 0 7.08788331898216 15091 113249 13 1887.62786202847 2677.97463726766 2289.86678811012 3287.96769373382 3323.63036493819 5046.78625803328 6585.33340044665 7951.84498497423 19529.6764492313 364148.975525355 51163.7444048424 7542.4257688241 35690.5837961023 15728.9569963794 23663.9591463674 187043.220311018 5540.2228357769 14078.3260392279 9926.9204985437 6448.68949972668 5810.47046030847 2895.97723314845 2525.220114912 2319.64888320171 1197.01728925041 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2436.69281614571 2054.32592097721 2854.07267627054 1366.41544299666 12988.016718773 9393.06025734746 791.530325383449 246.977123547827 0 8.47980058561441 14816 115906 75 2105.01910036337 2486.49510487628 1660.70893317285 2943.22601145985 3759.34977445397 6825.73238940288 9268.14896292428 6789.03751352274 28088.5832851991 382478.303837409 38517.9221175816 8177.09603291946 13709.6209145029 11154.6586526122 19633.0802190653 144253.53397488 6032.31254670072 26277.6163387733 13466.7682173141 11691.3643546117 7332.73776837597 1860.31723542927 2290.1209726998 3371.00080087503 1411.72312166401 4851.26653507142 9704.91695485482 1030.66104935709 420.370134971957 0 11.6857861125526 15278 118172 76 1875.95804950943 3060.77112015343 1848.21821165649 3235.72437545982 3235.236353293 4756.96863917696 9034.29301739857 7384.97708581068 20244.3445335145 412147.2175772 47761.7461852268 8122.0215956317 29047.3748939888 13788.5026554359 21885.4726110017 177876.664673412 4905.51522999438 27795.1309639266 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27491.9212141792 190468.121580497 7136.60532791676 25845.4387145165 12350.0526078794 10074.4101882528 6462.72141001493 2074.86581505157 2387.47904132683 2448.90342760225 1442.42808656934 23466.4281110361 9796.97139014205 911.723544619614 461.774027436063 0 8.13631894533328 15176 110434 79 1760.24583375467 2527.10462580948 1893.89218335387 2397.11334002693 5091.72243435442 5740.20756868234 6930.39612302569 11111.4643578077 23404.0223430402 354783.592947497 58519.809804937 8808.61259072846 21208.2931305441 14153.0841593706 19756.9483205055 168086.927814495 6112.68921440592 24360.9869354949 10108.1058515423 14211.0506883209 6185.46723529362 2585.30894624254 2604.43126047519 3207.26331592433 1407.21140771956 22569.4163318607 7776.07700725894 815.592034370836 218.947302774731 0 9.0399731377822 15032 110264 80 2113.20535689183 2838.41405038887 2151.13623209271 2719.46444540234 5356.4085643248 6412.84418650244 8135.06102020763 10513.3242473445 26517.2321733631 406661.657082924 74658.3354836696 8337.03240991401 23320.7208036401 13967.3366005745 25394.947901347 185482.039469634 6033.71062285335 21252.8676488547 12106.2901505007 19248.4592973947 6454.07407122501 2429.8450233529 2681.04935108985 3080.56330140618 1726.33292396553 14313.6437516858 8612.44019331536 763.33306712368 329.591151695729 0 9.2602404233854 15124 120846 81 2271.48914322956 2503.99428892844 2137.20015157472 4080.1360373857 4950.64009614495 5817.37741025585 10427.5467674315 9243.11945134538 21742.3596433629 383063.502150967 61481.323949348 9301.15336501787 32657.8263535978 15839.9763516755 25673.000441412 158393.960948793 5103.59227344511 11586.8031691753 11553.3382775266 9405.94003128229 5585.59437200256 3143.86033424962 2547.06472858402 3648.40626433404 1299.42124926424 13604.7538955359 6492.94567614018 972.68477004346 183.132073530794 0 10.2929391184717 15517 118198 82 1830.73936655713 2832.73646209988 2221.24796472958 3127.57421454846 3981.6109567526 6686.68625458564 9939.35380428702 8882.66006907419 20301.2670528344 363185.183667433 41938.9747370657 9204.33685199416 41408.3930285399 13705.719179649 22429.3115760231 165261.536289331 5128.2004624138 15314.4381685465 8884.77409741045 8322.37244144612 4961.1060836263 3048.22782586579 2439.46515086206 3288.24013563324 1182.62941880523 32740.7578633668 10302.20620955 952.937175167931 381.285939318132 0 9.52415541549867 15036 116408 83 2031.73972662887 2790.01814116499 2032.00041702984 2826.90987352803 3182.10688021148 5045.83638823734 5120.44809519355 7505.52895461908 24193.5221686537 388233.633074249 50513.7606915322 7627.85138177408 35070.9591544913 19677.1537971112 24192.3820378573 184824.341503732 7501.76585591101 18026.6661037786 11531.6434482402 4666.51699959607 5926.91771559306 2442.08058645248 2984.32313414464 3523.20896307972 1378.81556750231 13558.1970399266 7806.9832750153 632.765285832692 233.950182506952 0 9.06284133431475 15617 134033 84 1827.13788103591 2229.14623391099 1841.0893171414 3260.46058380801 2910.40476654796 4808.60307961596 7612.88031297083 7359.28759645244 25847.1610987649 342344.549779214 37216.5331187028 9166.10850481755 31373.6680350043 14412.9777478492 26020.4687427133 182859.527014085 5427.23323257395 19970.8528145807 10209.9868381742 10757.4414122973 6887.11248094949 2242.30673349803 2777.67274022054 2797.05760474185 1634.19387249408 3449.4177254612 9347.56761499326 543.017883390569 333.835584413001 0 8.13048289784951 15134 110469 85 2186.62467920797 3389.45598428922 2031.82193732548 3323.12592520993 3094.81696696227 6679.34777803808 9645.10849647657 6358.25586033125 20750.1829567425 413662.9818959 52483.1743042702 9108.6905326737 44919.9495652333 14645.5459495258 25552.8131324877 197370.64520652 5607.09176688531 19522.6530738537 9845.76513204027 5045.82101823421 5653.20859320858 2035.78893175036 3138.80601178173 2824.56435798326 1325.83089599614 13591.4803326046 8438.28989718432 636.957157096219 209.040907302573 0 9.01952090457547 15405 112275 86 1945.49051911185 3039.09710053881 2002.72110638193 3300.44510505138 5939.13910439527 5822.24455065498 9557.78004338605 11954.7955424997 24911.0699022049 427749.485446231 41019.556253175 9493.28530991542 35838.3111950851 13857.1572138856 26471.6168403293 175755.28053666 7324.53905092824 21287.1370374014 8305.83098065547 8212.21407489136 6208.14456252016 2295.7307657143 2630.81997769546 2945.06296078572 1466.62984769431 13860.3387134418 10282.4124164554 832.390803248232 296.419275446775 0 10.7019911766151 15322 127150 87 1999.75808083272 2958.49147436335 1924.69499758169 3200.97366840793 3056.08125458278 5047.45495017182 8427.4103773258 5891.03064151172 23453.9693094907 384972.026666844 68564.586433763 8388.68674293193 32745.4283617055 12135.686367339 24251.816619288 164186.776433083 6285.38223692397 18225.3829656659 10062.6474246642 11093.3310091916 5986.59676100972 2594.63262221382 2414.27811313027 3942.24758859938 1410.79394110045 22976.4449466651 8196.37780351799 780.202303467336 269.762286176125 0 9.83590752898663 14918 105638 88 1812.83078587 3657.4936129613 1936.84650113305 3401.2102612033 4340.17166092109 5458.77498802683 8205.85517329301 9089.58190569731 21533.361163029 409312.644751691 60711.743573603 8294.30602758859 31949.9379402986 13414.3554109568 27295.6930849246 187562.794638077 5593.29493061603 19530.7395689419 11594.8710984264 8371.11548842256 5600.13067590175 2613.4118047868 2750.87848424071 2769.51856191899 1736.72474291405 32629.1494042589 10783.7209271041 914.685725488114 87.7524105568152 0 9.37511822710713 15409 133568 89 2396.20063771843 3298.29285228401 2053.13841950979 3179.10334020122 6884.60819673594 5923.10932534485 8665.63371344957 13697.7841975451 21727.9018860901 406035.735019 55358.549067236 9524.68676277251 20755.6787754185 18073.9557940667 27430.3312611217 191882.658849053 7640.03966178126 17962.9341956088 10463.0923495578 13134.0896247399 4931.35575927316 2146.7395498497 2939.78400362507 2858.45056459257 1527.61938031776 13380.52346552 7840.94962863154 1188.35920938771 363.026690535959 0 9.21454038882811 14965 108021 90 1885.07239913285 2825.75535525827 2056.76378584605 3326.38054701899 4274.05299255843 5978.63259572623 7406.84899913987 8961.64481543487 22917.8493629789 385054.280787402 45318.1494142476 8797.11649005243 50420.1309788858 16531.0312808165 23485.4271861308 173804.212332622 5068.24847043568 18619.1591888859 8808.66059716239 10394.9167190604 6617.74978815148 3355.1195489397 2673.76046359156 2233.36474264196 1393.56512350838 3837.0135554711 10009.0918456617 537.552381157322 257.018220347439 0 9.50751628573606 15356 131472 91 1741.16518186831 3032.28822734125 2093.25088125203 2962.75897213353 3112.22765783346 5862.17374753535 7364.58534132463 7788.42604134286 30647.3241414439 425489.511672286 65399.690549278 9725.20328413826 50594.7735209881 14432.486683686 23873.5137839467 176118.980537962 7816.87773898936 24303.5571272968 12027.8768098134 16415.9085328955 7342.16560912525 2216.44856171957 3010.23257193528 3277.22672073372 1480.69353856291 3649.81174483791 9366.26950178612 1008.31820459487 217.992521919135 0 10.1730371843885 15083 115890 92 1752.53350108402 2271.2329888168 1972.24471748116 2702.50344589363 3343.97577973816 6365.41549309123 9463.92527535294 6869.81836847713 32423.754947916 406470.735318619 67921.7313431754 7715.05016578466 31455.1116695948 17461.2142068652 23607.4749052465 158944.469943275 5649.47348106836 21351.6866166784 9867.03622452857 5921.5871204598 5405.87755195558 2670.12884006054 2651.17314744473 3063.52049645489 1645.36173071781 3803.99930726251 8071.12647660623 814.261909971269 236.693229338633 0 10.705363984944 15250 127785 93 1921.83331520347 3151.64794801551 2201.94893439507 2521.14319218768 4142.92167936086 5317.9311560436 8526.40469313291 8475.52456772303 24151.5153049338 398234.592331029 40064.9972904905 9309.89341591145 22802.7094360193 11783.8840976156 26772.7743367212 195112.987850628 6780.94449190877 20591.4769528519 13015.0553044555 18204.2411439699 7200.84968979365 2726.15972697166 2575.79664406118 2628.89193536732 1177.40795893887 12646.2404599926 7483.31886430813 866.311585071916 187.882396362256 0 8.96416132186655 15042 128756 94 1783.87648686112 3063.09226062979 2587.34754885806 3376.33593412795 5737.07366931112 5584.25155532068 6652.59598282283 11207.2829285623 25800.164190312 414430.418568255 38640.9175282434 8660.86810223443 33654.9401319945 14637.8759921285 29340.3507130737 206416.557617337 6985.10292519747 24006.9126241764 11115.694332077 11082.4448954149 6091.59483541083 1887.1964475827 2286.50870537303 3505.5261338283 1394.15108347879 13434.51716365 10241.0993996868 622.359735447967 183.299914455592 0 8.79363216401104 15228 119827 95 2145.63837735273 2891.45452247527 1977.54976287936 2752.98760565012 6160.01814660033 5356.16054105617 7440.92645861141 11965.7526002685 23481.0912440241 366248.416126337 62020.7782017418 7116.6716617442 12345.6828132625 15814.7632295011 23099.5838058687 192293.898871934 6528.99077914356 15202.1564450012 13255.3574617032 10223.0042695355 6323.37678163005 2013.83189780067 2266.9053245385 3640.19079122231 2013.53366901942 12594.0257182259 10112.0875859169 1005.01501068462 112.803826984448 0 8.1532851499908 15069 98871 96 1833.4955151493 3470.41683857906 1953.01904242418 3482.80451268129 4221.97373206126 5537.76976337059 7850.57338210932 9358.28112546823 23639.5258255199 402432.383694366 58521.701008977 7940.48476976146 46181.0559405498 15151.2498813112 30164.7532998985 221607.72713428 7153.04468768664 24238.3495099744 12608.013555718 13940.3283985516 7551.77212526268 2755.56335789301 2512.46282430201 2722.30678317154 1625.36356728543 4012.80461491926 8936.69340646169 1053.01509851804 218.896300502828 0 7.76845375114861 15048 107569 97 2119.61425350978 3529.26154061015 1843.38100817941 2642.09165286371 3369.45547833767 5516.99090714315 8886.47158953601 9045.56669279377 18256.0573854099 355111.279961673 63267.1267203845 9187.23271158821 38190.8349147013 15872.5940614721 23595.2696924741 164276.800909624 3968.71905368155 23675.9856012995 11663.4431239112 13329.7770664195 7865.41893056995 1941.32579528846 2972.28631836203 2594.40984391852 1686.1623523747 13251.9966102333 8917.08283259926 1046.95492249136 189.712311496552 0 9.10256558038722 15117 131698 98 1742.36842141686 2306.24118043266 1852.66370249841 3021.65932855035 3091.09758224742 6518.61553745315 9292.73575364351 8699.14068508394 24114.2486143212 358461.884906496 52065.3581762589 9377.85313413267 40224.4311610879 15233.52736533 27652.1517474633 192387.551231265 5359.92149079207 25760.6695616147 11785.8837888643 16091.1974401627 8531.50959053226 2335.39197176931 2167.19723750504 3990.44381050681 1335.780201398 12980.022805105 9117.13114436517 808.625198780275 227.842594795113 0 7.9810881195601 15298 124444 99 2027.23726875402 2828.08632293351 2151.81278729849 2301.62160987145 4010.87410392073 5670.47836380688 9690.87182260637 10070.5646461405 23303.5014414894 378194.286570095 40028.7358872414 9017.05585467607 23947.1801224723 12874.8746366453 23834.4996280758 179411.168336216 9215.33406771591 21215.9784625769 13758.8172296503 15347.6153358126 6351.57344459016 2759.41355305649 2413.12212854957 3292.80477252657 1701.90747803392 42116.035517762 11592.8025818379 940.975833924897 166.327518122884 0 9.27706541179814 15136 109862 100 1930.07533422715 2706.55906324194 2319.73016057791 2553.68926372223 3101.63364725332 5292.87370860271 10050.7171380204 6986.29181383306 23251.8092516734 404241.187460877 47241.879240536 9583.98989016849 12214.8109057382 12718.3883708993 30952.7075119036 206517.172686359 5727.56919969937 15668.3913842597 11543.5321718603 12989.3811561055 4579.73504444153 2823.26140532217 2609.02532263695 3133.69244185478 1445.15051052689 22882.7614672319 10215.5588975127 898.708724288153 313.005951256852 0 8.52514052080536 15160 109175 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 1975.2172075535 2937.12043794332 2080.14566391036 3018.64702073121 4306.03394041569 5585.76160484167 8077.48186478101 9256.26186369634 23698.968369241 388359.499252647 52587.9254903539 8803.81587228577 31811.049056841 14863.0758619289 24670.791005672 180430.040173616 6211.66364226151 21057.8625871808 11062.2019090071 11759.979729983 6353.90220893813 2625.06502156875 2676.78155002764 2985.00952203367 1509.65723054463 15427.5010233001 9088.7491211475 831.26545472767 227.982666224736 0 9.33086464709441 15156.09 118546.74 DEV 218.887905624811 362.617826626996 205.907057909583 447.611417577898 967.442582778061 489.520927307679 1342.06162899524 1908.09278106951 3902.19538581137 26680.2383922072 11315.0448944739 812.704828514632 12701.5456599069 1851.10737166845 3281.53765592702 18046.3506840771 938.429187754873 3643.52763481831 1675.28445502466 4523.51724113138 804.125727215624 442.238067281002 368.988960761641 521.278158734256 188.458004575622 11258.5025978481 1151.24154614785 178.209675641589 84.6567079133078 0 1.02366379821736 191.587217475488 8574.67322015247 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # # Testing for significantly different ping-pong signatures (Mann-Whitney U-test) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + group1 (sRNA-ACR-140_preproc.map, sRNA-ACR-145_preproc.map, sRNA-ACR-150_preproc.map, sRNA-ACR-173_preproc.map, sRNA-ACR-178_preproc.map) vs. group2 (sRNA-POC-47_preproc.map, sRNA-POC-48_preproc.map, sRNA-POC-50_preproc.map, sRNA-POC-53_preproc.map, sRNA-POC-57_preproc.map) -> Scores for 10nt 5' overlaps are different with significance level: p<0.0001 (Z=27.36). Sorted scores and ranks for group1 2718012.92158017 1000 2711872.00120478 999 2700450.78450985 998 2666026.78371462 997 2664187.63402009 996 2657278.63952419 995 2629922.00512958 994 2625031.71390824 993 2616420.27938062 992 2611826.91345613 991 2597616.70489144 990 2596030.90962769 989 2593979.73824922 988 2589857.02973306 987 2585500.09953347 986 2573842.98411916 985 2565329.44806353 984 2558412.16911938 983 2554381.27985533 982 2552153.86363848 981 2551440.69804121 980 2547920.82870593 979 2547435.67322693 978 2545490.73768012 977 2540527.96549249 976 2533797.06792466 975 2532955.11806628 974 2530132.99793942 973 2528973.10129213 972 2528011.72246997 971 2527754.04787591 970 2526601.78387263 969 2525221.6572897 968 2517035.92901867 967 2516224.09939717 966 2515962.43522174 965 2512507.10396986 964 2509643.78223652 963 2504325.27548477 962 2503501.69006116 961 2499980.29086188 960 2499498.6477084 959 2496409.90429514 958 2495812.75265748 957 2493121.0221561 956 2492789.98530953 955 2489629.23508029 954 2488052.67639693 953 2485486.96516912 952 2482167.9534757 951 2480418.61482963 950 2479585.24750417 949 2476675.90966676 948 2473865.14037277 947 2473079.45221646 946 2470850.64362629 945 2468772.36445819 944 2465904.83880813 943 2462808.40399934 942 2462218.61332303 941 2462020.89819961 940 2458086.58393617 939 2456780.88553867 938 2456465.6074273 937 2452439.17315246 936 2452108.89752804 935 2451654.14274299 934 2450048.73916193 933 2449309.59885454 932 2444694.29907236 931 2435219.82597616 930 2434078.50826317 929 2432941.75380614 928 2431828.42325671 927 2431660.53718126 926 2429904.48844817 925 2429687.85993403 924 2425121.7354107 923 2424814.87849021 922 2421862.49145208 921 2417721.22936332 920 2416977.78698048 919 2414130.62005845 918 2413575.59036878 917 2412161.9695326 916 2410185.06618708 915 2408726.53098396 914 2404400.69084676 913 2397741.06365725 912 2394277.70748149 911 2384821.99357504 910 2384482.80418737 909 2374440.73586597 908 2368321.22212545 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