Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-173_preproc.map (7987330 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 36772.5277617648 19316.2339537702 10642.7520269067 28105.7201485728 22005.2817694551 30509.4783163383 35099.293890026 66492.0504959769 163123.763308846 2006466.67552895 135212.433198388 69104.6383726839 50012.2134545901 35246.9826043148 15677.2315637061 16810.9997460914 18430.3865410145 14717.3915726211 18760.4489427973 12317.8593089604 22390.8320625996 8328.93037576429 6707.61507661513 5940.92000928095 5463.4679451751 5377.88710918128 2742.62289249361 772.365263022309 286.512421866825 0 48.51649733468 55190 309684 2 37313.5535183784 17163.8061870022 13082.5340023917 26294.8382707308 21066.7196122805 31439.8661140435 39743.5544090079 67990.8711489013 165329.669213775 2022161.21204935 138679.626842911 71293.1296389873 45584.6914490961 34929.9280285727 16177.9499963462 19042.1322137507 17485.4416904496 15437.7202012743 19122.1381259033 11509.6726312794 21985.7278837159 6837.12022003723 5792.3648792318 6601.9773259609 5263.31377311443 4229.48041763255 2918.46848803724 1096.71171470038 681.900736350447 0 47.9548297204971 54838 315584 3 36746.8033667062 18072.4445308943 10716.3408715187 27823.967933277 19372.5161958869 30826.0468768381 40891.2580452066 62421.84282396 161135.712987022 1928719.24409613 129119.636926537 69744.249219713 47665.1720993839 30733.2417891006 15579.4930430517 18715.6804020657 16865.1162824888 17281.275823262 19663.004844068 11375.6627534391 22097.3070638569 7816.13572614027 5868.38310853831 5698.85768865236 5680.40285094748 3654.40641533622 2289.79918701819 849.929434489146 550.038786272883 0 47.9344493575873 54961 315008 4 39823.7393599447 16491.1705973258 11517.6617976196 25125.8098089945 21275.111537456 31103.4007362432 37548.9045028059 68518.2190085175 178412.584647967 1971602.44935691 136414.273156173 67495.2424999808 47403.3875199151 32832.9192751683 16164.2447814781 17674.1146905191 15348.2076670161 16000.0635849211 18648.8034169416 13517.5815392478 21446.9049678248 8679.53802658069 6253.50862658105 6564.27508054909 5721.21135219388 4046.52862213731 2809.04167850942 1367.17177752052 1240.67925039093 0 44.6753883216455 54934 312894 5 36569.4213583703 17859.7241361791 9697.01141232762 24362.8580940484 22846.9649992996 30985.673359054 36392.5180873618 55415.7245796391 158954.44441525 1936603.63566419 133145.513009615 71388.9866178802 46383.858915579 31150.8535591056 16423.536453955 17744.9263178023 16731.2040846131 15583.4056795755 18167.405427538 11680.4880700866 20972.7208290631 7712.79587626904 7211.9814906384 6267.2732953704 5359.33652989535 4523.61834490285 2542.18504144744 1165.42872299917 471.942696194859 0 47.9699063256004 55301 322168 6 40398.5041298401 19209.740138365 10153.1998979685 27156.391640965 21464.4009992427 32033.1188885156 40099.7840627363 61892.9179180383 169800.876596358 2006709.67037508 135164.696362592 68927.5970158917 48756.4103665067 34574.941938341 16025.6994692898 20165.3594423348 15285.6061193197 16480.8435570928 19809.5768120317 11540.8095561827 24973.5662658819 7010.1938657531 6381.90313323841 5899.19411504341 5132.22047867228 3854.58084449214 2927.05490013317 1385.55600525456 495.097711969721 0 47.5301774589175 55138 318760 7 38652.9289437116 17252.1082592395 11563.5577545826 23712.8002825478 21403.2698184655 33569.0827554244 37278.4112977596 67549.5050301351 161903.326086203 1970503.23203977 136474.348043817 76619.8863997343 44004.9468481811 32690.5368612588 16279.0552507896 16663.2786721963 18792.1778625649 15863.4363884127 20840.7641927404 12926.2119735464 24760.615947869 7743.36581067718 5735.46937809286 6039.03107430427 5462.23851502702 5221.85983081898 4205.69391492576 1064.27059227701 849.357724738439 0 47.3836345215714 54621 322575 8 39528.6976514675 21737.5930240777 11617.8732067229 33941.7527354402 22322.0161336644 36746.446695204 38154.7670388011 59220.5911927046 175215.370530518 1891612.52137827 132098.800940395 70211.8028699994 48174.7100878096 32329.2843049702 16306.5154447551 18337.7064596746 16469.2236470524 16280.9961595506 19260.2085620076 13080.8307403944 20076.7570636555 6549.92501146869 5697.07778583213 6398.40653838297 5501.36313275015 4412.82055674433 2196.99425312428 1201.94423128673 488.238685555962 0 44.4999285695406 54878 313177 9 37027.0301883879 16653.4986342431 10261.1995681885 30167.6047738947 20646.6364386775 31526.9199281625 36377.5701768217 70152.6320897429 176897.594373611 1894567.19877375 133223.010269216 73255.7038041329 45046.0812703299 32987.5729371678 17456.2683460832 18977.8494919158 15594.2920438055 15151.6161908671 19276.6734536309 12969.4769018209 23980.4553105355 7841.34362186692 5559.84927292063 6486.29930707501 5758.96077471167 4438.21921954231 2360.71172782324 1414.6063294865 611.352530050149 0 43.3382088707169 54697 311214 10 37880.5342214117 17134.315997275 14456.6851447609 28462.743172248 22312.1918728038 30129.3623424795 39109.4009098564 61922.3250018227 162014.097738646 1959082.26318246 131755.484886824 70848.0126817259 46980.6448459623 33140.7870073239 14960.8308394418 19489.0609365324 15945.5573331795 14048.4804641103 18733.9204645165 13748.8402377813 21222.990574441 7619.94396639936 5868.46179325776 6485.92994393612 5832.63419358464 4499.17932849837 2482.90601603432 1397.87983006512 482.816759903461 0 48.2987390529519 55115 312933 11 37184.9226295097 19001.8139533627 12088.6863124765 27579.8909352786 22921.2532693674 30162.5533965855 36070.8925929717 50525.5850861782 148880.155718313 2002510.02485967 141769.584106907 73957.9265432432 49289.0367879255 32703.7660249873 14853.0539724328 17244.958070998 15776.850851555 16281.7022543507 19023.6724677568 10922.4268643301 20763.8543087401 8302.58843776628 6537.10194701417 7613.58018929121 6696.69122706111 4755.69027803656 2925.16590150851 1552.83918469308 687.19493754149 0 50.2349747710241 55191 326210 12 36902.7215639626 17214.2635239759 10543.8392136358 26769.7401635501 19860.7156106532 31835.0967813223 42843.0547892087 54336.2996277088 156112.894456304 1934985.55227891 138993.23366095 74789.0545350576 44717.7615762374 31591.725884872 16829.6391432652 18161.7534294691 16707.1216960616 14512.6163868348 18500.9950094376 12464.0280295221 20818.6618438276 8155.06207956251 5466.50892741243 6571.22195003949 5222.95382418462 4516.97059462724 2314.00814184832 1608.30003119943 558.701011994132 0 47.4691009132757 54960 326471 13 33460.337172979 16240.9246803235 10895.9043081397 26499.4842834067 21777.4686301172 30766.0488033524 38017.02782646 56185.3210751541 158026.03596364 1985100.71442332 135933.387949294 71331.1066099293 46197.3216083913 33948.4709090477 14787.8053773841 19540.0208920531 20779.8178619232 14658.1421805503 18881.1614385095 13295.6053866705 23241.9533219039 7582.74758997236 5807.80603453901 6201.09068932338 5578.82650704552 5498.15026373079 2379.83941066413 1478.32331555093 455.694175697666 0 49.1597627286604 55209 310280 14 38358.248346672 17953.3673533821 11522.5306112301 30572.5535379917 20705.3963639235 32553.5959108443 38979.2703785381 57729.3638841023 167249.426507859 1942218.38029767 137574.645384329 77079.4278061064 46889.2183972927 35110.2036442734 15930.1885927732 18994.7306967188 15676.5663963641 15171.7849527902 18424.0062859637 12097.3776271504 22229.0485050954 7845.28597909419 7411.8933082031 5866.26350330816 5345.93070064211 4744.43121393179 2594.41788197486 1332.0407658472 471.588258979128 0 45.8331613086915 54486 316556 15 36165.4317789561 15583.770041643 10012.1254791895 26864.1952495802 20836.5949723643 31818.4095110084 36839.3963058436 65824.9284699599 166771.624661809 2002463.07829904 130213.457243497 68490.2182785986 44702.5005716632 35021.8393965315 16039.8191439241 16936.1643044526 16358.8004530367 16864.4665902302 17479.1056575688 11281.6378429692 22676.0279735128 8062.07035726342 6239.3295696376 6165.32483956263 5758.07202599091 4631.36300640937 2774.29062480155 1390.13549641286 859.730533866442 0 48.2709427058046 54309 318775 16 36526.5842861643 18349.6444854799 12286.3047748519 23314.0782249667 21090.7020997359 30325.2794379352 38006.3454909665 61595.2201160814 164132.88692324 1980025.07490169 131427.194144815 73924.0400445939 44707.296771093 31713.3178310416 16529.9594608977 19465.0667763046 17163.2661084741 15243.9507239848 18380.8675699123 11827.3924967166 19871.2974841601 8159.19894438181 5826.27214266603 6986.30489101107 5229.54829507073 5203.95213040631 2291.67044796835 1181.63546871335 1278.10659902784 0 48.1579158458073 54965 321362 17 39767.8354827297 19021.0426813567 10922.2217939863 28651.4968165377 22493.9460241842 29690.8248901739 39714.0377382475 59713.7996397614 154982.039027686 1971402.31597152 135210.411849915 74581.4683835467 46037.3322490145 31985.0551423386 17024.6043839237 18196.5923105841 15620.7487243023 15949.0436638864 18130.3206173757 13675.7046286481 24116.5889728374 8486.20361949489 6411.63715525658 5710.58972440084 5383.05470331843 4819.65016026062 2459.98612685026 1036.33122597274 425.997655843334 0 49.3147824272965 54575 311225 18 41170.9524650953 18306.2335843418 11106.6901349633 28467.3995518799 20688.1797049133 29183.7886923362 37468.4004259455 62680.3237751687 164273.321389291 1963917.4404264 137381.033065333 75739.9898950869 48826.4385999528 32798.6098362504 16996.2533859956 17191.7545285461 16724.2012845289 15225.4502306478 17414.051881216 12191.7941903998 22624.1148109168 8390.62008248766 6197.00954456853 6578.91791550745 5336.89333218567 4382.55296961404 2524.31210475047 1896.14192892549 529.79388257707 0 46.6986527695505 55072 308068 19 38265.8918841826 18770.7871447865 10642.1451526153 28090.7734032301 23400.9001306928 34470.5081993437 39876.7156460164 60746.5185007221 158217.284087548 1976216.09559116 133046.198340894 72936.8961834076 47862.5418876391 32491.7728852598 17187.1496008342 16462.7297022755 15413.2132050721 19746.9795094875 18309.2730641378 12872.4266494554 21705.0271692611 7939.56487654357 5385.03474982187 6759.01982829131 5719.93207350165 4151.31554235277 2491.31316525421 1172.53239504465 1072.17570164656 0 49.2989872547447 55248 312856 20 36690.6128870756 17389.7781691754 12676.1719043327 24796.1423952856 23295.3509292833 32512.0194827097 39125.9339460402 58086.7963878758 163119.570275139 2009549.48181062 131911.207652773 69359.252708418 45220.4624293937 33431.1966489724 17708.5020274526 20330.2966757821 15334.6457622041 15461.0660818398 19004.2351599982 14002.5554733414 20923.3662806468 7464.57664574523 6174.65248009045 5658.54848123396 5962.70072741683 5246.30687074787 2712.17918822582 1291.04926985916 793.514342405563 0 49.6372335800347 55289 316874 21 40137.9881631749 22925.4761859315 11733.5936452805 24945.3534738525 21339.4630477867 33003.7284820039 39205.3228286109 66259.6352210544 174642.360178519 2041795.37887878 133876.003426491 76441.0366481787 44830.0467032386 32641.0131400626 17979.7575745607 19268.5556452309 16354.8834992999 15037.3903664884 17709.3629824537 10562.8377739597 22084.8375025982 7766.22651199054 4933.61233958579 6610.63849678489 5345.98293765842 4723.96350929877 2610.91605654603 1085.33033211087 650.493550762413 0 47.2279058334996 55604 326603 22 35481.7170937802 17349.6811434522 11569.5993045042 31164.1191918649 21721.3873999802 31474.7076677843 40388.1590177655 63254.5953184065 188811.084793593 2071800.48548547 138648.415067414 70067.6675296848 42662.2617415158 30329.1245366004 17598.7315596067 19334.1337599596 16477.2569145783 16286.0556366795 19119.9596981165 11521.2790151822 19128.5787082007 7844.15192666125 6383.03033842863 5839.51170496222 5946.06999420989 4386.88195949687 3235.83840983889 1052.91677389841 1100.58692886528 0 45.1626619928686 54885 313390 23 35681.6223701523 18733.770646802 11525.0313930535 29103.0547512893 21506.4115761166 34066.7265788156 37857.3158061651 65115.5247552596 189822.15895548 1980262.27355856 130820.913301232 76645.0248160098 50901.585582606 32276.4050667678 15472.302164694 20190.2530527091 17662.2366962708 15859.1987507938 18656.1948964426 10884.6389963818 20560.8019868012 6995.25292953445 6752.15026604296 6221.29324445564 6069.63697185692 4616.23645506174 3061.08921157497 1023.05392776691 259.452532499693 0 43.454697681154 55429 325676 24 37993.0913812584 19174.2632705889 11846.7912368107 28130.1991504777 24006.1735873674 31420.5375599004 38165.413200472 58441.3432325972 169938.580322986 2044466.93171139 140920.181941172 71547.4061367932 50403.5053364488 35675.7672537047 16620.0028096204 18767.5122668103 16342.7691315539 15677.6489892882 18037.4296796226 11698.9035109525 21457.1088295942 7532.74970429533 6447.14137304277 5894.66073430723 5968.65953036633 5643.37377984069 2718.47677302142 1001.82391133647 334.537549979126 0 47.6616469148687 55525 324852 25 37094.5808367905 18186.9531840514 10823.2722213907 22828.8708380853 23002.232600654 32089.2498457008 36374.2675642898 55114.9315651963 183163.966443856 1950934.29422721 134196.048826144 68012.5019398571 48823.9631452375 30885.1644562878 17260.7074035271 19614.5120973623 17689.5714447972 14658.6506297993 20494.1522512627 11787.7651422633 23977.7207449026 9581.54952108794 6104.78472470782 5168.23599572808 5945.69394072622 5757.84057823697 2564.7317198623 1108.287337873 373.192172594644 0 44.0375534397502 54731 311572 26 34323.7638170142 21191.4733755309 12959.3247161175 26185.1316596585 22134.6848159477 35214.4440263981 36840.6136090999 65652.3427540553 197720.693495073 1901609.22096614 134386.022474893 70650.855467357 47079.2233938782 34170.7485953044 16934.6447911549 20108.8749362441 16190.0316682419 15084.5892559046 18654.6573843917 13447.7561614991 19703.2764105625 7370.16530029241 6157.16762748082 5376.35587595442 5249.14441328126 5236.74238109977 2420.52286069789 913.670639319719 864.331767725854 0 40.4447238826806 55459 324269 27 38288.3108637915 21484.0483310034 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16551.6670614614 19977.0574367542 14193.8078932162 13719.3735031383 19449.5201306427 11957.4328645476 21006.8930826989 7844.12731672133 5642.16013089266 5945.32586068526 4490.00250633321 5042.96296694641 2428.48570566771 951.333023140909 1021.10698690093 0 50.738338619909 55012 311621 96 35897.3735716177 18724.2137708485 11270.0052174122 27065.425708614 22506.041200442 33984.6848852506 37557.7561697972 67226.5764553158 161759.640465322 1920093.73379748 135023.617299731 73590.1863358267 46931.4260492517 34136.2045097246 15396.1036107478 18257.047015216 13401.6301896744 15969.9562471391 19204.5508097376 12254.8027076069 22253.2428063725 8325.05066516588 6515.59588311187 7009.61423898496 5206.8504021916 4666.74020222663 2849.74910756198 768.027585912056 395.088229010149 0 46.4198405953422 55637 315493 97 40760.9489538124 21352.2204862972 11120.3188690243 30666.0639729603 20773.9050390732 34105.5647235667 36382.3600198134 57430.9658826249 163072.477480775 1903433.92114615 132670.904168707 75203.4835443887 45210.2531376738 34075.4011757734 17904.0891681936 18801.7187234921 14948.9526158398 14779.8171428019 20715.8972224827 13827.3264455585 19661.3334934696 6599.2579776973 5484.15689411927 6334.90238605974 6502.44936117764 4396.36806214975 2534.46503682569 693.923074669826 661.218280024477 0 46.6942684474132 55297 318029 98 38616.0798624839 20329.0726537539 13211.2744239569 30710.2505954982 19358.2036794752 30797.272533764 36858.7433526431 62812.7074796173 159294.043539564 1901408.91630387 130339.700874373 74562.0554605881 49488.5866558455 32944.1610873778 17840.4147247676 18924.746261037 17531.4304310996 16020.4673947793 19028.1337705999 11580.5860436721 22064.9623725742 8409.45674655703 5863.3823914287 6194.40046594388 5186.79302688522 4375.09127330215 2625.48910215211 1222.83208479742 708.517864986245 0 47.4783309862696 55287 315662 99 39241.2429713764 19249.7908167205 10825.5244760505 26043.9699894222 20692.3724928181 32753.8000135949 37692.5401126446 69241.8634869985 157859.112617962 1915833.66891053 134465.321822154 74506.5208781522 48471.8938814529 35166.1244305096 18067.2562722944 19905.8290084237 17807.560211481 15316.6081242392 20331.7552997259 12232.5372662987 23462.1842101556 7319.67591399351 6042.28438893705 6533.51633414092 4960.14329518205 4322.99745733614 2439.16091211933 1282.96067852558 286.660177698752 0 47.2386113481844 54767 306635 100 38236.3035888583 16980.6145740915 10576.4445807553 30916.4944681844 21357.1672356946 32921.0137521622 35375.7486120733 59591.8252820928 166327.160899967 1872111.94992499 137564.226580458 76912.7494911375 49032.2278751827 37506.6544499301 17138.8496248844 20108.9639178184 17604.8005550727 15702.8552642746 19852.4582929199 12101.7509881798 20948.7828689047 8584.13621227471 6088.95335671208 7152.64705239869 5068.52472026174 5237.07284498689 2491.66463635411 1109.76343221704 1156.32980027804 0 44.4036665355475 55243 324178 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 37966.4675596094 18531.9998445536 11417.5320666778 27786.7468299616 21619.506444315 31999.1212527067 38269.6499057532 61326.2784860903 168376.318826771 1965659.74086886 135715.261799749 72963.429529523 47106.3608916399 33595.7205755569 16426.8263448748 18707.6896016851 16595.7357408826 15731.6714555062 18760.2832464286 12209.7171611641 21850.5020565353 7820.33304640562 6238.24583329911 6266.71498337639 5512.02897310494 4712.70375804757 2648.28902849789 1163.873154664 668.824771897251 0 46.6037189240698 55031.45 316115.35 DEV 1823.73600740948 1683.63970956343 953.844481174936 2645.13162138575 1262.45761393756 1563.72635886792 2117.53478571193 4591.15700203291 12570.8747472985 53857.304942312 4605.87527406694 3357.59802085769 1871.17336114856 1894.16553740143 902.260610308301 1245.70081658842 1528.06155955675 957.53718268386 859.318578572709 1014.69751629256 1340.29398123901 803.217127310019 513.684288895494 581.413838896436 589.463231945797 510.147078480517 428.90606541616 277.017422409764 284.246723690616 0 2.32658692927183 315.885434137125 6592.88790497002 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #