Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-145_preproc.map (8414719 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 38124.5584957518 10057.0183753722 11433.4889617406 11738.3006153328 31562.911852527 31576.7254965659 51437.957829033 124262.124050342 147510.424661382 2452108.89752804 174012.478502065 56294.3872419822 124073.61916524 26046.6292561669 46305.8239703522 20556.9564893859 20998.2286657839 70474.5084988278 25546.7584179135 18150.2686468912 19005.3131221081 6972.36921530757 12572.1341053481 9668.88327990828 8848.36977645628 10589.1567384753 3875.61910996872 1761.41719840146 358.492597273819 0 49.1536137199023 63995 318296 2 47458.2519065189 8979.34365655817 12547.4642553193 12658.5541774792 31171.9979926256 31950.4289529312 51438.6728377383 141275.725454842 118803.140748963 2397741.06365725 164949.283173241 54924.1931176831 128823.334485168 23647.0732405497 46091.4741271746 18653.6824327734 21036.2648508752 80083.7897709774 27333.6303002428 16157.1417255485 22513.6646414695 7726.67709880424 11558.1605423355 10083.6627678399 7840.62504151914 9895.47941150187 4078.56612162323 1515.62507756948 287.01212918261 0 49.6167331773789 64790 324682 3 41597.7147607066 9358.20314024323 13707.94130418 12270.5302809912 35434.5176686832 30858.2198173603 54101.440383916 131007.16557681 140425.025254532 2664187.63402009 156668.817121085 57068.8246953767 126283.495841309 25581.0385857566 49367.2869338966 21421.3009885339 20377.85269358 71403.211268842 23479.206909783 18462.1939371703 21577.105306852 7114.43755905262 13233.5578864342 10785.2043777147 8988.48548922436 8531.77407694999 5306.72565291612 1458.01967488192 368.021835886013 0 56.1232065574982 64733 323524 4 48050.478425454 10218.5034406498 17908.2607444527 11851.1436582914 32369.4290355867 29109.2958417598 52499.9115073478 130112.495607866 129814.15158951 2479585.24750417 158819.10721976 57523.4685405518 127446.666080099 24718.6934017053 44233.9738297926 19846.203050023 20674.4879431212 71628.0051517798 23426.8791542707 17814.6614647899 20201.1490179225 8205.78731590143 12351.810218162 9690.76796510572 8245.1530426915 9897.18807847546 4121.09807329095 1711.06371995045 423.271686370159 0 52.967902299025 64810 320242 5 39227.6977056438 9942.92635437188 15102.1504781635 13819.0514707626 30127.1090160331 30844.3931357953 52168.376304769 133296.358632042 148674.173799085 2492789.98530953 155518.606389758 60935.1872778123 125803.615792474 26488.6366661307 46432.811622369 21097.9232386506 23454.8671090567 71478.8473090063 27523.4756138103 17616.5171236815 19412.3969921893 8207.21227246216 11988.4538465183 9937.86237612605 8633.09249652493 9367.86432535648 4571.35125198089 1703.21703924256 451.797506200263 0 51.6680133734419 64701 322804 6 44942.0094199624 10190.0540332172 14215.316850745 11804.0197756144 32019.0865939598 30873.4915087151 55923.6938326838 130183.938819736 139904.635127252 2589857.02973306 169478.241176632 57098.6863759341 121571.478289763 23842.0523987303 47088.891378446 18945.0092758692 19615.095892136 74737.5561083892 25219.91552139 16774.7962862585 21152.6507716552 6572.84225570255 12086.7270581798 10530.6662020542 8219.35825933461 8039.00494567775 5010.4441142218 1793.89473443105 304.427670282935 0 52.9924495885943 64705 327981 7 44974.3648862222 9092.23102007864 13686.0219885072 12642.0310601209 36040.5121050802 29779.7065638526 57895.2832893546 136456.086586286 131733.036208059 2451654.14274299 176781.460785731 56986.4052950446 121732.825155305 27246.9645237544 47397.6753400824 20603.8229925198 19792.973516359 68352.0797734883 23766.7396673017 17618.2538297394 23016.9207215738 8494.65436775163 12525.1905714502 9315.62589074687 8996.31770975159 9247.83922986628 4424.9049980634 1905.27421997177 434.117465559359 0 49.6130887964214 64566 319494 8 52661.212505326 9304.39791474326 15284.6381528305 14114.9434986661 30931.6471536481 32688.9165827722 53667.5875515474 144432.166701461 125458.980452046 2434078.50826317 165683.10107347 61563.9511700114 127115.165750413 26179.0180240622 46234.3451254302 20527.3879031816 17757.6662425345 74168.0436516458 27120.415942331 18208.1954410216 20955.1237780057 7770.54470439227 12391.2985038531 8824.70353873519 7871.48931273051 10039.0532284781 3490.31359358962 1563.06489570033 414.949906442912 0 50.0813010311303 64340 314896 9 41807.8541121997 8004.00388964082 15007.1924697013 12994.3043842515 29643.7573935052 33409.0709333037 56154.1472349175 130943.594417678 153684.643361219 2480418.61482963 161209.24722206 61308.9815586225 142262.143872388 25660.403441335 47687.1740436954 20796.0814344551 20696.9411054461 68586.8720390034 26093.4429613262 15042.5881025796 19588.6197473531 7306.11890640396 11109.3109817875 10057.5885336859 8733.12813493688 7613.71034384856 4191.15763138618 1963.08808444528 291.234690038698 0 48.6657275064579 64299 319535 10 44741.4670309632 8433.76894687421 14154.4735629516 12018.5498530961 34400.8894700568 32939.4513555264 55326.3550614422 116512.342262145 105986.526286026 2421862.49145208 145974.806624835 65260.4345012328 123484.682180405 27150.5844852682 45037.4992535003 18302.0283052924 20728.9798367129 73122.4645575507 28221.14985375 16988.8404258556 19231.8953727994 8277.15847305101 12773.9358771483 9915.07031631603 9241.69641396634 10681.617225472 3818.57480703962 1398.11242261014 454.186666605421 0 57.8577043666081 64583 315015 11 39034.4850415861 9118.73366415643 14272.3072481302 12044.5391151849 32032.9629704063 33646.8839529545 56009.643736376 123662.457063926 127030.689245387 2528011.72246997 141939.838918283 53966.0778899883 120502.272900566 24364.1880429975 45173.7349980875 18843.1560356899 21286.6033897508 78164.2231312931 25547.9205643695 16376.6045951127 20839.9008486869 7682.61570326904 13130.9888485725 10889.2450340803 9326.86528726747 8426.45551054874 4560.17463611093 1849.58351754726 350.175600267845 0 57.8204207144407 64460 316900 12 43624.7659769219 8040.76004815655 14083.6642742325 12322.890908045 31478.9070846619 36554.8822316002 55799.7115012018 129196.866152608 133233.808997718 2629922.00512958 146775.658276118 55130.1536099116 122008.972334748 24242.3969780266 44131.3432050055 20351.4541346908 17000.3353790354 67675.0520777422 26548.0872467595 16701.3472276845 19131.2946878051 8372.66969881448 11703.0803644192 9502.57208215636 7571.51769972641 9325.74532987352 4671.04705552844 1511.50788770533 292.995468420891 0 58.1438253811575 64142 323426 13 44090.8315981845 9015.88072118686 14247.4015844985 12337.4276348761 32182.9101236379 28497.6123974523 58094.5525410706 133484.224817144 143926.292949665 2450048.73916193 160351.313460585 57169.9357649762 119873.657625561 26709.3188492096 41375.0687563239 20622.7564298956 21251.0085242092 63355.5647124268 24635.653206021 16887.6683372431 21705.6492657369 8493.69921281632 11918.7374659543 9473.07112934125 9120.96414160532 8490.18513823766 3881.32393340444 1618.14422380867 285.576996315404 0 50.9216296488559 64207 317394 14 40626.2008482979 8592.25923578985 15737.7189836052 13211.9041720986 32401.6765491527 30337.2372521622 52588.3714985663 134960.911192388 124093.502560781 2449309.59885454 159554.623278108 63180.1819652035 115037.182275581 26817.874344815 44810.2997187304 22553.0892127682 21271.03795058 73029.9178831795 25267.6217009219 18474.2776787705 19392.6878726706 7740.37709060368 13107.4168753137 9527.53060215635 7761.99789502841 10163.2751907881 3460.45506078898 1632.10877723678 295.510017704392 0 53.4603137969056 64517 321675 15 42988.2485910698 8044.91644841519 14453.991241793 12652.6760448405 32589.0810104168 32300.9406361638 57022.5686820578 123563.977949434 114592.918948875 2585500.09953347 153698.508769837 52651.2223101241 125113.489680841 28599.6892500368 48544.0802720724 19593.0408168459 23641.7434789632 79158.7873876152 24298.5590951442 17212.2398926953 19727.4615230986 7712.26920767425 12590.7607105468 8716.17614796043 7748.94962810358 8377.08992303801 3832.17181079661 2004.55469654961 356.025354420001 0 58.5089760116739 63732 309955 16 48333.4456975537 9351.99357090535 14104.127656257 12954.5282622444 29185.3769756731 30708.0360487582 55009.1370981311 134039.733948583 111254.527123777 2512507.10396986 160174.614649709 60806.5722183048 112784.087949428 24969.7104277938 47177.1778968201 20227.3771656459 21607.4742819824 70733.0104901336 23272.1130432568 15484.1538373206 19852.1954074361 6923.58798658324 11938.5676909824 9650.85761929919 10024.1701960928 8801.39067587668 5744.49343254656 1615.23902572288 340.756341010901 0 55.9846675756473 64190 317045 17 39495.4966369679 8336.54927123818 14003.6381105016 14008.6584469923 32470.6893106408 29707.9101803201 55491.7211917996 129227.360740774 130566.659206856 2504325.27548477 168370.252040979 53722.721196151 124069.175481753 25648.6795936787 46873.0954897357 19195.3890617479 19407.2137385606 72611.7485915263 26872.6733819837 16207.8927709633 20812.2363731374 6912.16894241093 11995.8411357678 9342.05552432682 8452.64084374348 12288.8012445089 4920.10919296263 1605.24080274798 348.449377486105 0 52.218643465364 64514 319117 18 43374.0138471013 8510.70467586302 12732.6820406106 14097.4919746083 30158.6754393685 29807.2358966095 53147.3054487677 124809.116048509 139082.284025093 2530132.99793942 163419.333452631 59189.4577802736 119900.843367388 24775.3471039142 45847.2468666036 18440.2697203435 18856.1451609226 67620.155688237 25574.7625263392 15725.1422944316 19502.1955763803 8667.24239203715 11169.1619770738 10087.2182625262 9434.28744047521 9986.21626920084 4487.4580343511 1645.96731364124 394.623416644684 0 53.2516948352557 65015 323931 19 48769.6374620699 7692.2929806123 13931.9732822292 12748.0360171327 31149.7360201279 40206.2833459276 56301.1051520456 141820.475604153 144201.594500474 2547435.67322693 164417.960673516 56453.1222989003 123471.9753479 24741.1875513763 43923.0829201771 20263.9752656696 19197.6252800402 75208.8876649824 24823.9063856896 16700.2780933956 22049.0323158933 7265.15104185611 11664.4805197178 9392.16541407877 9077.34969058839 11351.6978510987 3527.93736353834 2083.11114629062 540.590711399902 0 51.2005620813364 65010 317457 20 45059.2260796584 7958.36142072839 13647.663712251 13544.3556166796 31110.105561012 36831.274800976 54850.1250647312 128614.828463682 153582.362198616 2488052.67639693 145476.648886047 63440.5526654646 126329.125807376 24984.2613487709 46533.9852647406 19686.2396553249 19230.1242109802 71139.9840648309 24947.4701339677 16877.8376794971 20151.3630051848 7293.61111556442 12312.703175949 10534.7563047296 8200.47940131413 9942.08202841503 4414.16183776819 1417.11150634604 264.130394483066 0 52.2251953469728 64432 318557 21 47606.9208434871 9253.50314678402 13840.0588272798 12914.6944426051 31885.9679582059 30011.8806203987 51981.8814996437 141717.984447615 154272.708525376 2444694.29907236 162319.817736947 59702.4160520843 118050.581296516 25650.3013202245 44693.642208824 20143.4363711505 20943.6928843833 66815.3489229618 27160.0463799103 17718.4815421261 21662.5677139446 7348.43006727474 12202.7513754795 9239.39327987344 8288.91348690965 8564.41510313275 4339.7787910938 1800.72780190156 393.991049770194 0 48.922460867182 64706 322670 22 41964.1333345822 8002.96799409526 16517.7050246873 13614.4584219373 30825.1652080428 34001.5812716712 55683.9154430781 137593.138021484 123740.629064498 2473079.45221646 161299.613530262 56417.388820377 120134.39630853 25266.8779170277 46408.4586324382 22072.7572952013 19121.9996193348 71332.4246093239 26132.7358605377 15287.7352814607 20438.1179050262 7544.5726639223 12343.778440006 9914.95903686988 9361.64259328265 9575.02463582889 4126.99113142146 1525.50272760189 488.182645492351 0 52.9385224210177 64364 321985 23 48405.4010640933 10236.61168764 14615.8273925951 12340.9253703939 31448.7851390134 45264.4848485155 53606.8902806949 140366.990016714 127560.452782331 2309637.35854332 144224.467380193 58877.7998264282 123918.892794701 25102.5413383499 46750.1157600496 23333.1586356154 21207.0446140164 73619.8985123749 26080.6370187899 17350.3612958614 20715.7956809246 7724.8590001552 12441.3988867312 9472.74242583484 10056.8781924078 11401.9157245926 5280.98000210067 1556.25522115245 385.991149526432 0 50.999917491987 64297 319863 24 45349.5759490441 9051.2819273837 16453.2587107865 14052.0430694739 33868.8107343934 40514.7303016334 51675.5228455804 136935.216335059 118773.43461087 2384482.80418737 161977.804616748 59272.064616309 119867.432049986 27458.2742461511 46669.5470283922 20049.0616747697 20156.2138572658 67752.5594176826 21974.0100553988 16437.5005139516 19473.3644310059 8316.21856355974 11726.0523010036 9188.01422115497 9881.0547511458 9550.17307980106 3520.80002200138 1446.77519437517 307.291748267301 0 51.786212851376 64449 320250 25 45786.5427022405 8460.09714471475 14614.8375516182 11648.588857565 31774.0806282393 28948.7354709907 50799.3783024047 146316.773743532 122260.391520567 2456780.88553867 153206.05767604 62390.6952245979 115761.645794671 25194.9679443768 45931.1134794117 20361.5126648666 20472.1763791184 69798.0829640897 22527.2412496555 14665.2111830917 21914.9935020596 8075.18574738717 12599.2501556188 8756.79652635652 8321.59171529684 10082.6690894417 3700.64933758464 1564.87332965681 355.225979758294 0 52.7469528679022 63935 316751 26 38240.0475136885 9549.0847412948 17197.7129291787 12529.402247323 32073.9379689017 32347.9178797639 53538.6839872803 138721.114592323 127731.168985563 2452439.17315246 169400.186038835 62545.7050265839 113008.314449352 26154.3232880424 45616.4457336012 20325.2299148674 19641.8494141383 65544.9694902152 22730.9448049955 18255.316853095 18536.5417438075 7475.3916711505 10835.5120501426 9722.34656567086 8322.79600238763 9137.11218418134 4950.13474109014 1612.82607042691 366.734853322179 0 51.4548922186036 64453 317244 27 39688.9167690637 8510.08913934769 13544.619319711 12659.4578982871 33388.0149842908 31266.8857230014 55979.206934329 129551.749720538 120630.100116063 2565329.44806353 186870.242527376 62112.4583951515 125328.347535972 27887.7256560213 45530.8532343457 21602.8700974357 19483.2468381115 68482.0445189225 22311.642927569 18892.6368449001 18622.9725795067 7469.0719498244 12935.9796202306 9330.8088636227 10214.6222681895 10328.0102818612 4687.36227271121 1448.12238917612 267.452271184319 0 51.6150124300675 64410 318339 28 54981.1381621974 8661.48641677245 16236.7431536673 14709.6558534264 32132.8536137708 31081.5512563948 54298.8066847888 132162.239239503 131000.508671217 2432941.75380614 154592.45882401 63637.6176351901 123055.977953713 24301.0836021438 50270.662927879 19042.3438297374 17765.2058304999 81570.097985119 25051.149133694 16330.337616591 20360.1768034928 8618.09573747974 11683.6144216393 9700.42715677568 7506.78421376163 9544.24945256548 4584.83353861864 1941.25305626562 419.682241344689 0 52.1698645079824 64561 325494 29 41620.2514584592 9052.72132068425 13245.1336944058 12023.5284697122 31382.821431213 33216.2826293037 54390.220614638 122930.448414003 103205.931030825 2499980.29086188 151997.501879832 55421.3212661265 125014.499358581 25350.7750286582 49285.7626463048 19235.639556086 19149.5675791835 65932.3989878106 24286.4282767561 16635.634806904 20223.6300977766 7528.76938632118 12150.5770942604 9673.67719831366 8466.87546749388 8286.77033644826 3678.2122328644 1699.94645657499 278.403640926275 0 57.9697519107505 64237 317750 30 39318.3481172887 9442.42294794533 13853.3396913741 10731.181363718 36509.3982169965 28552.5565937238 55738.2081978081 130162.83575975 126496.273977026 2231197.18469668 156297.853494022 57875.5907473386 117829.668010829 24652.7649036135 48308.8114321821 19001.5187365081 22231.8503503111 72674.9529910338 24503.1958329726 17555.0305208659 19789.8955125336 7625.13522578791 12671.8841964152 10081.8400819022 7692.88737200595 9770.3173156191 5057.11489356135 1838.89629836283 301.985623480643 0 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124545.00358979 25720.5942310144 45849.149675424 21889.1879696194 20001.2861062575 76217.043718285 24391.7072696466 17822.6259267238 18825.2037249158 8058.01053776645 11840.3148435966 9808.50607324594 8022.03692178595 10058.0140254435 5275.24831989225 1495.88056014934 303.131822343146 0 54.1383090447358 64715 322548 96 42941.1300566159 9043.30561289338 12640.3579778974 10574.2937433381 33360.9494705696 28688.9136699802 53043.7702777944 139520.592406611 131153.230872039 2525221.6572897 139759.12301151 60438.2939561415 123551.925140871 24223.3844601753 45816.6908001893 21592.3526030644 20791.9619179305 67035.0299354517 24350.5707061363 17116.0481512573 18508.4721514923 7242.81077955449 13005.3511681964 9123.75653474904 8137.74245947347 9597.89982016519 4602.07760242544 1522.70425401441 408.654491229285 0 55.4270757444458 64452 318895 97 44159.1933047281 8514.4894132367 15319.1455175718 13017.8610376161 32338.7865644348 31118.6779595832 55454.9459916391 121165.79316077 150826.42482088 2465904.83880813 164331.040059627 63833.21071386 129499.518271284 25928.2550787943 49142.4459991435 21686.7103024562 21024.2529984872 72042.9570314802 25724.7275540329 18532.285222447 19691.0759290915 8947.61461029416 12931.860168608 9396.95057195599 8081.32535821937 11441.4313018875 5519.50546159704 1584.51818222925 354.668479914827 0 50.3980115115027 65211 324448 98 45527.7097087323 9487.50892223396 12686.4090685017 13630.4580541701 32947.524928504 31019.5397838468 54555.0283953299 167966.206331618 131803.881582687 2516224.09939717 161994.990481618 58806.5689104498 121251.766230205 23533.4504805497 43253.1874819942 21111.5544000855 20995.2836070399 63875.7462483099 23577.8550477849 16753.9473795532 20054.6720549343 7925.69151114807 10351.2301201529 9684.72780492327 8732.52294777689 9063.86250275424 4806.49217782314 1532.66703739977 238.105297564717 0 49.3738916874224 64293 315573 99 48302.9281405485 9976.71503234515 15928.3897030796 14643.7656123216 33553.6595699087 35716.1374883356 51505.8378684486 138645.173646741 129776.096565627 2545490.73768012 167395.628443912 56472.8836536774 117468.000469404 21951.9166343444 42880.3254465302 20381.6558741809 20945.9807093045 65751.9011374537 23536.3742548196 17118.3801437979 21320.2147429054 8220.48657037533 12070.149598726 11009.8005338609 7129.82329978479 10688.6599053031 4308.72979202126 1785.31928313032 404.360243138965 0 53.4163549488645 64853 323612 100 42578.616181168 7989.79087693059 13099.4425128381 12782.4356539426 32728.177387351 36369.8962283736 54473.441475612 131751.007980694 138649.304540907 2404400.69084676 161192.30696334 57435.6259090139 137765.103351752 24816.7991270208 48041.2203413906 18969.6884078258 20738.9807945966 75049.4246577404 26842.0678782494 16694.4939796386 21624.8908320103 8217.55184642619 13421.3688115583 10910.1466111365 8651.30795261738 11743.9053552775 3936.83333308135 1661.62106041344 310.81366494449 0 48.8897064001458 64350 324659 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 45274.6403641471 8717.55293198017 14192.9576600736 12591.0338419893 32830.5871251901 33418.4365344502 54167.6105345372 133959.614407736 133418.988375637 2489305.44665048 161156.265735015 58965.1135811589 123991.497531854 25007.9132780744 46427.4330148607 20194.4652578002 20253.5136280383 71212.6647333819 24819.4229931977 17133.2069232117 20294.9700479866 7838.78839130989 12166.4202260563 9748.6592986076 8628.71037137162 9637.77092030815 4313.5745527431 1667.56476058671 353.617884299572 0 52.2130943010635 64361.34 319154.68 DEV 4934.20617719818 761.54911433089 1235.58951293981 934.759188164381 1625.63928743389 3544.93860638131 2330.93801295188 7993.48314782824 12958.6997355376 87232.8326483773 9294.04629410275 3517.77313211631 6022.16078882566 1514.30911068324 2504.75288187567 1185.57550756635 1367.27854334037 4362.75569830888 1540.28449561659 1059.32704433376 1155.4906119318 568.696786613314 997.5883919646 639.096615062766 736.607177713208 1043.1995720441 622.538833734649 188.544069047189 73.625194950995 0 2.75905188275164 389.940180540554 4201.24504136571 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #