Ping-pong signatures for 100 pseudo-replicate datasets (1000000 reads each) based on sRNA-ACR-140_preproc.map (7898972 reads) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads 1 7344.19900759328 9554.62794889149 10068.2137019766 13929.1721471451 28111.7816134261 19184.0534223279 37880.5573657569 69070.3708793474 111577.50196225 1774209.68973882 119955.257444674 149348.605462194 35739.0662499566 28869.0068374168 29946.6779194349 12772.8549777938 19499.5611150387 13005.3465437595 20964.2882138154 11841.1464918489 8921.39763550138 8632.18183619604 8575.02297713423 6018.69212865567 5655.34771087239 6163.22755153894 2503.19880793506 1612.77237115959 449.728596640694 0 42.4391930773633 53904 260929 2 6953.98524990333 8195.54286648235 10368.6374179578 14722.5046739099 24741.4469310812 21379.2751806204 35938.2176672078 65793.6777852503 121412.093817456 1734396.82766259 115905.041458892 136666.350966767 32257.1123704011 28630.3969381085 28897.312239673 14222.7640884218 20431.0586738769 12948.2934067446 24835.9494499787 12814.955816334 8501.98455129903 7272.20712930143 9423.76602700271 5500.27303022404 7275.84030274431 6116.68074481507 2933.45240362956 1827.0941432882 772.463694317882 0 42.9236184204574 53527 258632 3 8010.8512148919 8761.0529757406 10636.865518486 15410.104006279 24339.6058813997 19919.3043889557 38316.3203524756 52700.1865912985 110531.234045117 1661379.63299767 105274.179503022 130208.863024277 34344.2145003734 26864.0691891325 35667.0603406743 13876.6785536727 19397.4852748473 12855.251577181 29337.5184262903 12978.6242562744 10424.9914251125 8101.51329880555 7941.47605494722 4941.66147447121 7515.97545527719 7896.84072428765 3145.92252393554 1705.95982614089 679.108209906319 0 45.2929254773114 54222 265228 4 7756.0567412197 6841.14175521144 11063.8152660013 14155.7154231063 26511.5981975104 19432.2110287054 34446.9453514187 54023.1581326157 98329.8041694467 1680997.77648587 107127.99968772 135941.94384271 32588.7475646541 28235.706556666 29563.5043296981 13437.075398889 19127.5914491279 12356.0520226719 25846.646276236 12315.9636396528 10543.7346435246 7136.03747241461 9029.26759332536 6309.7854912076 8022.7595969602 6883.50890871308 3473.85337114534 1590.86520607404 669.555092362743 0 45.802295250942 54045 263782 5 8700.5832433407 9990.59744869708 11735.3955777745 14747.9609979703 29988.8714348667 20184.0827136278 38650.5066996122 68321.4726331761 93412.9166036898 1741898.95706128 118126.713781058 127893.383310213 37354.728800066 27204.3430776445 27048.2361737011 14077.0512217035 21783.038722631 13799.9185452184 23832.5734818496 11567.6192905623 10223.0313251468 8720.39215661597 8568.40836356183 5922.20108695852 6542.14281496973 7345.35821136361 2888.16360756435 1457.28476003188 595.651362097537 0 47.647220463601 54080 260509 6 7698.50804742937 9763.61070889731 10450.4546684253 12971.7062234609 29020.6275357917 21005.5177941264 38411.4723573413 67326.1552206093 135268.646909559 1755688.11030097 116682.383732994 137335.146257136 35696.1654611593 29327.4924752441 31029.1416079216 14205.1667511208 20961.529284363 12588.059929803 23841.9867805606 12908.3481854411 9314.985689401 8871.75562247863 9507.60803852785 5237.92032073977 9412.59676990372 6207.89059460511 4643.24261695277 1250.88548806588 710.942052153038 0 41.66307980041 53912 255115 7 7769.97708151681 8957.13752605436 10501.1894183052 12521.0494494395 27220.5708829933 19328.1535177738 35126.5309116405 55086.1051184731 100737.079372672 1788025.76644439 119677.377879112 131009.560833989 36307.9050352357 28635.3814748137 28026.1253997646 14093.2078582312 19691.7070094301 12317.6050145111 24439.4361392638 13414.1855323382 8394.2576992781 7705.8712287597 8003.21735948273 4716.23401684734 6482.3020528398 8043.12707854589 3070.8619184124 1673.35643372055 381.311361632773 0 47.594061786516 53993 260986 8 7272.44115721295 8917.7405885748 10824.3388434751 13726.0528152728 27107.7974316411 22450.9042698541 35545.5198920999 61778.6074271492 109509.043004116 1773925.31381912 123452.544465266 111296.626750059 35243.0664183137 25987.8384093049 30276.7177606876 13563.0644385892 20916.2431211489 12948.0430490259 28152.3554119001 10953.8683862995 9515.1179621246 8156.48981586164 9605.38921720708 6966.53148413471 7381.66699683609 5703.08251859164 2268.95160606239 1740.61625643838 477.814463246953 0 48.4720371806695 53420 259185 9 8713.05796390458 10208.7978468679 10237.5445569317 16034.1661100974 26795.4122216399 21392.9789471386 39649.8203879451 62574.3772169709 122870.116058722 1578974.52754768 109929.024607611 141009.376475609 34975.0934172067 26750.4449570605 29873.3666432723 12975.8331413438 20072.336461934 13532.0433513885 22738.5086035086 12467.0390932347 9354.92944180911 6875.11071469241 11185.3753178716 6910.47278870618 6965.02899684333 6558.5177151331 2941.85579945804 1556.08337229958 755.568586896619 0 39.0870808865478 53667 258290 10 6490.47097130086 7664.5863036786 10298.2000381083 14847.5130593686 26698.0690461453 22029.3353584737 35801.9323458294 63994.2298497224 105452.849532252 1629588.91184397 114601.235475631 123924.346828586 35264.9739219012 30034.0171423675 28130.8009575369 14263.461026472 23176.8891885814 13865.7481527268 26460.9023330251 10893.2406562468 10623.5752015628 8087.1514636124 9987.60117476793 6341.22257227321 7284.32745532793 6016.41350864856 4062.11188425557 1496.59445612309 547.550593572427 0 44.3535388906742 53508 258256 11 7332.33729833661 8295.02444595018 10959.6602384644 14652.2513519702 27839.9167799667 19812.6591413287 37819.0476955745 58941.2435864491 93652.1461643097 1746487.17058068 113715.723094182 136782.613636187 33671.8487953902 27484.3860200643 32878.3832499025 12983.8961509113 20472.5011696776 12402.5895187969 20839.5155634641 12037.52858746 8686.78556419738 8119.67935949078 10293.7145738983 6162.06045527259 6159.19040628457 5648.81295250074 2418.12062095595 1533.13003579469 762.475541584215 0 46.8919449861969 53661 259486 12 8396.70356613958 7924.54747624085 11797.3231718732 14762.0608051752 25043.3008854205 20741.462970005 36848.3733500677 53066.4325321732 103843.584925729 1540351.3626459 102211.429888156 126962.206694076 34746.8153669326 28635.7592268646 32147.1814242737 13529.1271701649 21688.3490952902 12517.686226916 22854.494690277 11405.4650589248 8613.07815163311 8980.35795712323 9490.5680664693 6445.04357464308 8016.43583054789 6219.25602051134 3258.79389961494 1428.01870781109 510.95072059764 0 43.6100062813274 53845 259954 13 7096.69263548661 7701.70904536969 10225.1965464605 14083.5119899883 25088.0061611201 19081.0468389145 36407.5962255805 57215.2192834362 96415.791132948 1681189.63892381 108246.598617524 128035.671546686 33368.3918584315 27192.9909208617 28740.4140551183 13815.8659216382 19742.4967982657 12730.4681977816 24624.1802375523 12616.8135923655 8374.95379265779 7887.11247799671 11946.0196138722 7936.33105502683 9296.21035291559 5589.68484659185 5703.72551863123 1509.66589096459 985.799076941584 0 47.1979684084658 53331 259697 14 6257.72212224538 8812.00892435293 9780.47436400503 14359.9628225581 26714.4371855893 17946.8202497307 36096.5728565336 53572.8004216245 112605.025841849 1692877.34788892 125249.53279424 144075.126863022 33369.3675055879 30267.0091447933 32401.0024263174 13899.531563971 20921.2000282676 14086.5885828638 22851.4834669201 12116.893896083 10007.6642614077 8156.24826910395 8494.87396727906 5706.85830556421 8380.58472352144 6332.77088150612 3277.73421731388 1533.97944431361 547.02687744531 0 41.0398978067003 54257 257223 15 7484.46762194399 9895.30097777506 10319.8632657432 13983.4275140238 27156.6737309093 20062.1722365881 37493.0721299632 63230.3123959871 109128.16407396 1772173.48845505 118958.93723967 126376.905285765 33256.1698561495 26533.3912915398 31147.7117481916 13086.2797662016 17191.7985939674 10952.3312105884 20283.4813440055 10985.5342481646 9270.02155247876 7869.72501652685 10688.2522363767 5929.96041621283 6819.41041676754 6414.90457382266 2758.08682414656 1549.4080187953 552.854976693233 0 46.382839919647 53901 262947 16 8162.0468238829 7948.18494098157 10775.5889590266 14549.9431813118 25651.4851164892 19365.0598067175 36584.0617061876 56578.1056590609 92978.1399756317 1650097.73635843 113816.18013937 130593.839956231 32374.8950093214 29968.9263187061 31687.6476195215 16411.8528670651 18015.2934547776 14108.4152868752 29523.4273454708 13641.9265849553 8548.54196691383 7506.25266925256 10084.294781738 5814.02401286368 8480.01727655655 8128.15302183292 5415.74925346943 1578.27238054069 553.112287108705 0 45.8740230680905 53879 257703 17 7940.81001979251 10632.4723727291 10946.0587575211 13609.4335053574 25475.2900307712 19862.4271058831 38614.7907745294 61505.8901945269 98703.2718431528 1797400.22689009 113782.507942773 128772.79803308 35610.6617329577 31609.1045393894 31447.7231453554 12717.4616921043 21356.160826607 12851.0181657094 24145.2682682852 12209.5050179841 9055.06943220809 6951.83992206038 9200.3631777731 6091.87281829657 8101.62538973782 6474.90851668203 3397.14285187679 1583.94389198884 762.717154831163 0 49.338568440402 53896 259803 18 7401.67935871657 9983.89957736344 12041.8803894781 14919.2159911095 25335.089400596 21640.018675846 38856.9654132851 57706.2213653247 100721.40071014 1708994.88365559 114103.095000397 138064.127791799 33861.1021842828 27612.0472720631 34540.9597249876 14649.6558958005 21176.4527779642 12873.3849562736 26346.2777256179 13341.5360001386 8960.80088762374 8582.28353546255 8735.54440656603 6442.16087154193 6399.9231387679 6345.23656337646 3212.63671969137 1677.54462800461 904.752138869025 0 45.3213711530196 54001 257306 19 7040.38183975278 8729.92514019547 10946.4091739333 15583.7683758554 25777.6210890552 19530.5544134725 37179.1000816547 56911.0289575311 83646.1064552773 1646393.50103067 106805.718338794 134403.55353676 32711.3005934745 28192.1922540575 28510.5339971789 14390.7378533099 21196.847529913 14950.7533534472 23407.763291826 13594.1027152634 9472.87410178938 9519.83799047094 12069.4284061266 6210.76722718207 9847.94830011812 5676.31790708297 4929.1258505539 1687.8522247519 574.45958014293 0 46.9800327409943 53773 256469 20 9147.24773358983 10227.7293321855 10377.4471272527 15399.9502493048 28171.0370662945 19241.496617475 34760.7196027025 50225.3909548592 103214.985058127 1608063.93442791 105439.007083313 138326.937359374 33832.7815054357 28280.3899005591 26785.6233756552 12629.2939929563 18173.8543549642 13454.1708333394 24719.8630403668 11443.5440959719 9067.21139369241 7487.84160536088 8442.58753462113 4782.56737415779 5605.65884449504 5098.13867871925 2299.00296709285 1403.0546199486 744.818103738933 0 43.264637750335 53610 259373 21 7935.20206512213 8503.46095799711 10460.142247627 12746.2588030717 29248.6442556784 18216.6058247807 37279.4002900416 58639.1106382021 102593.710644716 1783267.39310619 120390.462723712 132130.555792766 32739.436125517 25941.7006965757 27380.3728480375 13095.3267017282 20640.4425942612 13361.8760047222 22310.4527111084 12051.8366751838 9707.37559950704 8119.81017723713 8293.73218472029 5514.35999090483 8931.23021140919 5926.23201722074 4001.73362707129 1353.87174992527 481.431965555964 0 46.662295894029 53746 256170 22 6667.98895529864 8307.54240067656 10461.5665514794 12511.3563138257 28972.7417599965 18249.9974582758 34265.4322369361 60043.7777546132 120991.803513619 1662846.47657585 123299.274305312 137916.829925409 35662.0387520637 25663.6609624565 27724.7162414202 15093.3628455785 20551.5967412216 14214.5894623957 26557.908571768 12967.8923143587 10999.5495375726 7314.86239239464 11065.9260180957 6289.05710616852 6186.90516433837 5579.9645492022 2281.70309062161 1747.63331261115 588.954035522359 0 40.3405888089569 53513 259394 23 7239.58053580304 6880.22024199507 10707.9738190347 14325.5516769789 26024.4767987645 17705.333763607 38737.8654270949 60281.9051442751 94356.0458219104 1775514.66764349 117573.101905033 125045.862598964 33080.8560213952 26422.00873475 26812.3982462824 14450.2165143286 18038.6490869013 14466.3593728216 25271.5396524701 11186.3465171427 8548.00168523008 8022.33243647856 9426.89569017492 5431.69438746801 6810.7435000846 7171.0282886919 3639.38564476525 1791.5799721361 443.386619972136 0 48.9680359636134 53741 255250 24 7449.08099774067 9337.28921641058 11827.8355900062 15842.8503735744 26291.933135562 21232.4032761012 36441.8831714751 57223.3528655956 112793.15380143 1694311.04985389 115175.856210623 109264.890895672 35039.458176334 30502.7075212752 25450.5131971703 13346.0063307401 18927.6395506787 13867.8735855479 26335.7840035429 15002.1002565408 8474.90801526162 6455.83156996155 10504.8735422756 6207.11598470478 6010.90734891345 7444.76737888235 2997.46417293274 1255.28046985672 695.809206823182 0 47.8777658255147 53952 263606 25 8500.85583554936 8215.11465507817 11436.1762550244 14939.9343681503 25102.352804559 19845.3019346665 38785.9637426216 54760.4680285466 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12955.6899778527 20804.6059416928 13383.0679875067 23413.3422820922 12475.8926210296 9190.1896209366 7164.21983278065 9910.28015738442 8300.20768201022 7618.21879917525 7815.83475403047 3382.03281446869 1449.69816708434 689.164266496748 0 45.4281581818782 53911 257734 96 7890.18855361294 7947.18046510767 11377.7715711533 13621.6813008992 26201.0357226386 20207.5711315667 35754.5276822802 60391.5036336344 116448.201213455 1724104.83752902 113128.761544295 140205.151879523 38178.5118767464 28646.5969434071 35086.6948177506 13447.4866525508 20181.8758531843 13794.8193544916 24241.3701711597 11780.3200079272 9360.93272551226 7760.14431549052 8378.33457287883 5898.72576708015 6416.84648144756 7506.32619504394 3106.91794562173 1814.39548805072 467.515932281497 0 43.2974078618741 53493 256664 97 9019.68599505142 7938.31862852504 11442.5744897024 13675.5936486343 29060.5594634924 21463.815938087 39220.145052897 55933.5307420031 97556.4650250263 1770302.16742649 118632.485377359 127684.772898856 30931.174610928 27710.3236892997 26133.2970013306 11059.4194495172 19245.9149502489 12447.0218581839 25242.5267133738 14163.1650540785 8921.69406784142 8405.01019929328 8191.39263679604 4852.91640467554 6014.14697951252 7195.79704167336 2709.76161228625 1294.29447161103 743.398284648467 0 48.2379973562657 53321 255757 98 8184.1074498695 8012.83101639836 11397.803236937 13264.1757523695 24939.5065057292 19756.888120296 37904.9948336298 51833.3901720517 102363.433367704 1719386.32803173 111501.08913709 115580.538271913 34057.0676717034 25407.3589323797 30344.1789391535 13290.1139659493 19913.6903616302 13225.7010173819 24481.7367429651 13070.8684764786 8854.01303699144 8018.70644583177 8947.93471956585 5677.77999796471 6169.60325219145 5620.21440021678 2583.31354929644 1733.1563668438 532.871778997223 0 49.642874291664 53775 260533 99 7306.1634117109 8413.96475745857 10885.7772088071 13026.2316578328 23126.1467705561 19271.5446917081 34554.4493876322 53565.2099343311 113029.009433074 1711643.38879883 110538.778360717 133787.40830014 35549.9843898228 28407.2474677931 27986.4775558349 12613.5760561752 20848.4196318373 13489.4386614724 25401.0256888737 12752.2439531925 11502.2420311127 7585.91257162864 9082.67686824721 6216.58065104991 6519.58946671409 6749.93178476722 2963.15580816472 1403.62168879338 550.300956202734 0 44.7656721097837 53564 254150 100 7454.45429087431 8370.75598825261 12794.2756003848 14096.6460001162 25839.0895072203 21427.4613803214 37335.5301165781 56648.4120445761 111384.16531471 1792928.31318977 117701.607487056 119117.10819057 34461.1669031891 27630.5371734629 26406.657833279 13820.8375097595 19926.3121540795 13137.0576236326 24067.212896475 12457.4281228529 8228.68026905886 7067.85226675656 10914.7141067801 7139.58133472505 6833.12046534269 8374.64839884632 3758.4414830801 1797.87677966318 802.582562941844 0 48.6799262506654 53813 263891 Average (AVG) ping-pong signature and standard deviation (DEV) per [nt] 5' overlap (last 3 columns refer to avg. ppZScore, avg. ppSequeces and avg. ppReads incl. standard deviation). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 ppZScore ppSequences ppReads AVG 7687.09413139014 8673.17237952825 11082.2110192901 14354.9673919161 26797.562406392 20370.1768877432 36892.6968628949 58637.987600435 100357.00304638 1708755.19467126 116133.407912363 133178.299695755 34054.8120774112 28575.0554403324 29901.7000115487 13673.1255513557 19919.9930534731 13097.3343328709 24254.1378367715 12566.968254734 9181.20025291687 7893.34797779992 9580.21293714654 6216.90257249442 7259.12975897672 6655.82099957338 3275.99918365534 1525.98993265422 657.137570210966 0 45.5294464423446 53793.84 259236.93 DEV 608.236565897121 871.283670223808 654.690977544641 1192.41411145664 1487.74421487993 1186.84520531777 1590.85651344258 4885.96439847267 10390.5792785095 61616.8067503602 5163.67679281753 9536.48612519782 1618.68694404332 1457.54578119806 2691.69531155485 982.799033377549 1246.28954190093 908.692303365263 2119.83326671009 1019.53892545652 814.691376634633 787.129723443205 941.624502802597 813.106603384401 977.688969354795 848.448283077063 747.447689512579 174.280655970941 157.65028351858 0 2.45680377671887 325.532078910819 2915.49459013389 # # # # # # # # # # # # # # # # # # # # # # # # # # # # # #