diamond v2.1.8.162 (C) Max Planck Society for the Advancement of Science, Benjamin Buchfink, University of Tuebingen Documentation, support and updates available at http://www.diamondsearch.org Please cite: http://dx.doi.org/10.1038/s41592-021-01101-x Nature Methods (2021) #CPU threads: 40 Scoring parameters: (Matrix=BLOSUM62 Lambda=0.267 K=0.041 Penalties=11/1) Temporary directory: ../output/30.00-Ptua-transcriptome-GOslims #Target sequences to report alignments for: 1 Opening the database... [0.105s] Database: ../../M-multi-species/data/blastdbs/20250620-diamond.dmnd (type: Diamond database, sequences: 573661, letters: 207922125) Block size = 15000000000 Opening the input file... [0.063s] Opening the output file... [0s] Loading query sequences... [2.044s] Masking queries... [0.987s] Algorithm: Double-indexed Building query histograms... [1.389s] Seeking in database... [0s] Loading reference sequences... [0.727s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 1/4. Building reference seed array... [0.464s] Building query seed array... [0.222s] Computing hash join... [0.113s] Masking low complexity seeds... [0.02s] Searching alignments... [0.316s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 2/4. Building reference seed array... [0.239s] Building query seed array... [0.148s] Computing hash join... [0.081s] Masking low complexity seeds... [0.019s] Searching alignments... [0.305s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 3/4. Building reference seed array... [0.289s] Building query seed array... [0.163s] Computing hash join... [0.079s] Masking low complexity seeds... [0.021s] Searching alignments... [0.318s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 1/16, index chunk 4/4. Building reference seed array... [0.214s] Building query seed array... [0.143s] Computing hash join... [0.076s] Masking low complexity seeds... [0.021s] Searching alignments... [0.308s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 1/4. Building reference seed array... [0.217s] Building query seed array... [0.124s] Computing hash join... [0.076s] Masking low complexity seeds... [0.019s] Searching alignments... [0.262s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 2/4. Building reference seed array... [0.265s] Building query seed array... [0.134s] Computing hash join... [0.074s] Masking low complexity seeds... [0.02s] Searching alignments... [0.273s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 3/4. Building reference seed array... [0.236s] Building query seed array... [0.129s] Computing hash join... [0.076s] Masking low complexity seeds... [0.022s] Searching alignments... [0.282s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 2/16, index chunk 4/4. Building reference seed array... [0.214s] Building query seed array... [0.137s] Computing hash join... [0.071s] Masking low complexity seeds... [0.02s] Searching alignments... [0.268s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 1/4. Building reference seed array... [0.226s] Building query seed array... [0.132s] Computing hash join... [0.076s] Masking low complexity seeds... [0.02s] Searching alignments... [0.277s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 2/4. Building reference seed array... [0.244s] Building query seed array... [0.15s] Computing hash join... [0.077s] Masking low complexity seeds... [0.019s] Searching alignments... [0.29s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 3/4. Building reference seed array... [0.273s] Building query seed array... [0.145s] Computing hash join... [0.079s] Masking low complexity seeds... [0.027s] Searching alignments... [0.303s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 3/16, index chunk 4/4. Building reference seed array... [0.21s] Building query seed array... [0.128s] Computing hash join... [0.076s] Masking low complexity seeds... [0.025s] Searching alignments... [0.211s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 1/4. Building reference seed array... [0.133s] Building query seed array... [0.086s] Computing hash join... [0.065s] Masking low complexity seeds... [0.015s] Searching alignments... [0.189s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 2/4. Building reference seed array... [0.162s] Building query seed array... [0.093s] Computing hash join... [0.067s] Masking low complexity seeds... [0.017s] Searching alignments... [0.193s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 3/4. Building reference seed array... [0.164s] Building query seed array... [0.097s] Computing hash join... [0.067s] Masking low complexity seeds... [0.015s] Searching alignments... [0.195s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 4/16, index chunk 4/4. Building reference seed array... [0.132s] Building query seed array... [0.086s] Computing hash join... [0.068s] Masking low complexity seeds... [0.017s] Searching alignments... [0.257s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 1/4. Building reference seed array... [0.227s] Building query seed array... [0.132s] Computing hash join... [0.076s] Masking low complexity seeds... [0.02s] Searching alignments... [0.261s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 2/4. Building reference seed array... [0.224s] Building query seed array... [0.13s] Computing hash join... [0.074s] Masking low complexity seeds... [0.022s] Searching alignments... [0.28s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 3/4. Building reference seed array... [0.246s] Building query seed array... [0.137s] Computing hash join... [0.072s] Masking low complexity seeds... [0.022s] Searching alignments... [0.298s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 5/16, index chunk 4/4. Building reference seed array... [0.223s] Building query seed array... [0.137s] Computing hash join... [0.075s] Masking low complexity seeds... [0.021s] Searching alignments... [0.262s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 1/4. Building reference seed array... [0.217s] Building query seed array... [0.138s] Computing hash join... [0.075s] Masking low complexity seeds... [0.018s] Searching alignments... [0.273s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 2/4. Building reference seed array... [0.264s] Building query seed array... [0.154s] Computing hash join... [0.075s] Masking low complexity seeds... [0.018s] Searching alignments... [0.255s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 3/4. Building reference seed array... [0.248s] Building query seed array... [0.127s] Computing hash join... [0.074s] Masking low complexity seeds... [0.019s] Searching alignments... [0.27s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 6/16, index chunk 4/4. Building reference seed array... [0.204s] Building query seed array... [0.13s] Computing hash join... [0.075s] Masking low complexity seeds... [0.023s] Searching alignments... [0.286s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 1/4. Building reference seed array... [0.195s] Building query seed array... [0.128s] Computing hash join... [0.075s] Masking low complexity seeds... [0.018s] Searching alignments... [0.247s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 2/4. Building reference seed array... [0.251s] Building query seed array... [0.137s] Computing hash join... [0.077s] Masking low complexity seeds... [0.019s] Searching alignments... [0.256s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 3/4. Building reference seed array... [0.242s] Building query seed array... [0.147s] Computing hash join... [0.074s] Masking low complexity seeds... [0.021s] Searching alignments... [0.242s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 7/16, index chunk 4/4. Building reference seed array... [0.219s] Building query seed array... [0.123s] Computing hash join... [0.074s] Masking low complexity seeds... [0.02s] Searching alignments... [0.259s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 1/4. Building reference seed array... [0.227s] Building query seed array... [0.136s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.26s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 2/4. Building reference seed array... [0.257s] Building query seed array... [0.135s] Computing hash join... [0.07s] Masking low complexity seeds... [0.021s] Searching alignments... [0.28s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 3/4. Building reference seed array... [0.255s] Building query seed array... [0.132s] Computing hash join... [0.073s] Masking low complexity seeds... [0.019s] Searching alignments... [0.264s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 8/16, index chunk 4/4. Building reference seed array... [0.211s] Building query seed array... [0.148s] Computing hash join... [0.079s] Masking low complexity seeds... [0.018s] Searching alignments... [0.249s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 1/4. Building reference seed array... [0.189s] Building query seed array... [0.129s] Computing hash join... [0.077s] Masking low complexity seeds... [0.019s] Searching alignments... [0.281s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 2/4. Building reference seed array... [0.253s] Building query seed array... [0.156s] Computing hash join... [0.073s] Masking low complexity seeds... [0.021s] Searching alignments... [0.288s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 3/4. Building reference seed array... [0.273s] Building query seed array... [0.153s] Computing hash join... [0.077s] Masking low complexity seeds... [0.019s] Searching alignments... [0.288s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 9/16, index chunk 4/4. Building reference seed array... [0.208s] Building query seed array... [0.122s] Computing hash join... [0.076s] Masking low complexity seeds... [0.021s] Searching alignments... [0.279s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 1/4. Building reference seed array... [0.215s] Building query seed array... [0.116s] Computing hash join... [0.073s] Masking low complexity seeds... [0.023s] Searching alignments... [0.251s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 2/4. Building reference seed array... [0.252s] Building query seed array... [0.142s] Computing hash join... [0.072s] Masking low complexity seeds... [0.018s] Searching alignments... [0.247s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 3/4. Building reference seed array... [0.258s] Building query seed array... [0.13s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.276s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 10/16, index chunk 4/4. Building reference seed array... [0.2s] Building query seed array... [0.128s] Computing hash join... [0.075s] Masking low complexity seeds... [0.02s] Searching alignments... [0.249s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 1/4. Building reference seed array... [0.214s] Building query seed array... [0.132s] Computing hash join... [0.077s] Masking low complexity seeds... [0.019s] Searching alignments... [0.276s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 2/4. Building reference seed array... [0.265s] Building query seed array... [0.138s] Computing hash join... [0.073s] Masking low complexity seeds... [0.02s] Searching alignments... [0.264s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 3/4. Building reference seed array... [0.256s] Building query seed array... [0.152s] Computing hash join... [0.074s] Masking low complexity seeds... [0.02s] Searching alignments... [0.29s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 11/16, index chunk 4/4. Building reference seed array... [0.206s] Building query seed array... [0.121s] Computing hash join... [0.075s] Masking low complexity seeds... [0.022s] Searching alignments... [0.265s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 1/4. Building reference seed array... [0.202s] Building query seed array... [0.113s] Computing hash join... [0.069s] Masking low complexity seeds... [0.02s] Searching alignments... [0.257s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 2/4. Building reference seed array... [0.249s] Building query seed array... [0.146s] Computing hash join... [0.072s] Masking low complexity seeds... [0.019s] Searching alignments... [0.252s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 3/4. Building reference seed array... [0.265s] Building query seed array... [0.142s] Computing hash join... [0.074s] Masking low complexity seeds... [0.02s] Searching alignments... [0.267s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 12/16, index chunk 4/4. Building reference seed array... [0.205s] Building query seed array... [0.135s] Computing hash join... [0.076s] Masking low complexity seeds... [0.018s] Searching alignments... [0.243s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 1/4. Building reference seed array... [0.222s] Building query seed array... [0.134s] Computing hash join... [0.071s] Masking low complexity seeds... [0.019s] Searching alignments... [0.269s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 2/4. Building reference seed array... [0.231s] Building query seed array... [0.122s] Computing hash join... [0.074s] Masking low complexity seeds... [0.022s] Searching alignments... [0.269s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 3/4. Building reference seed array... [0.265s] Building query seed array... [0.149s] Computing hash join... [0.074s] Masking low complexity seeds... [0.019s] Searching alignments... [0.258s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 13/16, index chunk 4/4. Building reference seed array... [0.205s] Building query seed array... [0.136s] Computing hash join... [0.074s] Masking low complexity seeds... [0.021s] Searching alignments... [0.255s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 1/4. Building reference seed array... [0.219s] Building query seed array... [0.132s] Computing hash join... [0.081s] Masking low complexity seeds... [0.023s] Searching alignments... [0.257s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 2/4. Building reference seed array... [0.251s] Building query seed array... [0.15s] Computing hash join... [0.074s] Masking low complexity seeds... [0.021s] Searching alignments... [0.255s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 3/4. Building reference seed array... [0.264s] Building query seed array... [0.14s] Computing hash join... [0.075s] Masking low complexity seeds... [0.019s] Searching alignments... [0.281s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 14/16, index chunk 4/4. Building reference seed array... [0.225s] Building query seed array... [0.13s] Computing hash join... [0.076s] Masking low complexity seeds... [0.019s] Searching alignments... [0.281s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 1/4. Building reference seed array... [0.223s] Building query seed array... [0.131s] Computing hash join... [0.073s] Masking low complexity seeds... [0.02s] Searching alignments... [0.291s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 2/4. Building reference seed array... [0.238s] Building query seed array... [0.148s] Computing hash join... [0.078s] Masking low complexity seeds... [0.018s] Searching alignments... [0.256s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 3/4. Building reference seed array... [0.272s] Building query seed array... [0.141s] Computing hash join... [0.075s] Masking low complexity seeds... [0.022s] Searching alignments... [0.264s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 15/16, index chunk 4/4. Building reference seed array... [0.21s] Building query seed array... [0.137s] Computing hash join... [0.073s] Masking low complexity seeds... [0.019s] Searching alignments... [0.254s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 1/4. Building reference seed array... [0.194s] Building query seed array... [0.105s] Computing hash join... [0.069s] Masking low complexity seeds... [0.028s] Searching alignments... [0.258s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 2/4. Building reference seed array... [0.235s] Building query seed array... [0.136s] Computing hash join... [0.074s] Masking low complexity seeds... [0.021s] Searching alignments... [0.277s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 3/4. Building reference seed array... [0.247s] Building query seed array... [0.126s] Computing hash join... [0.074s] Masking low complexity seeds... [0.019s] Searching alignments... [0.247s] Deallocating memory... [0s] Processing query block 1, reference block 1/1, shape 16/16, index chunk 4/4. Building reference seed array... [0.198s] Building query seed array... [0.107s] Computing hash join... [0.069s] Masking low complexity seeds... [0.017s] Searching alignments... [0.246s] Deallocating memory... [0s] Loading trace points... [0.221s] Sorting trace points... [0.037s] Computing alignments... [9.584s] Deallocating buffers... [0.011s] Loading trace points... [0s] Loading reference sequences... [0s] Deallocating buffers... [0s] Deallocating queries... [0.034s] Loading query sequences... [0s] Closing the input file... [0s] Closing the output file... [0.001s] Closing the database... [0.005s] Cleaning up... [0s] Total time = 65.318s Reported 8924 pairwise alignments, 8924 HSPs. 8924 queries aligned.