FastQCFastQC Report
Wed 16 Apr 2025
POR-82-TP2_R2.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-82-TP2_R2.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224200417
Total Bases22.9 Gbp
Sequences flagged as poor quality0
Sequence length15-141
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6972700.3110029897937255No Hit
AGTGTATGGCCCAGAGGTATCCAGGAGATCGGAAGAGCGTCGTGTAGGGA3923430.17499655230346872Illumina Single End PCR Primer 1 (100% over 25bp)
AGTGTTATGGCCCAGAGGTATCCAGGAGATCGGAAGAGCGTCGTGTAGGG2336490.10421434675565301Illumina Single End PCR Primer 1 (100% over 24bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)