FastQCFastQC Report
Thu 10 Apr 2025
trimmed_POR-82-TP2_S8_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POR-82-TP2_S8_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263184807
Total Bases26.9 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20128850.7648180846548639No Hit
CCTAATGATACGGCGACCACCG13223190.5024298382087078No Hit
TCCTAATGATACGGCGACCACCG10475400.39802449538813994No Hit
CCCTAATGATACGGCGACCACCG7503670.2851103027387139No Hit
ACCTAATGATACGGCGACCACCG6871730.2610990382890909No Hit
ACTAATGATACGGCGACCACCG5339560.20288253189326388No Hit
GCCTAATGATACGGCGACCACCG5142890.19540983610045545No Hit
CTTAATGATACGGCGACCACCG4976580.1890907023367804No Hit
TCTAATGATACGGCGACCACCG4967850.18875899625923317No Hit
TAATGATACGGCGACCACCG4883950.18557112227226702No Hit
GTAGGGAAAGAGTGTATGGCCCAGAGGTATCCAGG4855860.18450381142251876No Hit
CTCTAATGATACGGCGACCACCG4456910.1693452616358664No Hit
CTAATGATACGGCGACCACCG3337410.12680861171442925No Hit
GCTAATGATACGGCGACCACCG2994070.11376302584214142No Hit
CCTTAATGATACGGCGACCACCG2794950.10619723956937985No Hit
GTAGGGAAAGAGTGTTATGGCCCAGAGGTATCCAGG2672150.10153131673744373No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)