FastQCFastQC Report
Thu 10 Apr 2025
trimmed_POR-76-TP2_S6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POR-76-TP2_S6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173500998
Total Bases17 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGG4389700.2530071901949521No Hit
CGGTGGTCGCCGTATCATTAGGA4276910.24650636303544493No Hit
CGGTGGTCGCCGTATCATTAAG3790940.21849672588050473No Hit
CGGTGGTCGCCGTATCATTA3739200.21551461046927234No Hit
CGGTGGTCGCCGTATCATTAGGT3106100.179024906819268No Hit
CGGTGGTCGCCGTATCATTAGGG2786820.1606227071961857No Hit
CGGTGGTCGCCGTATCATTAGT2672040.15400718329009266No Hit
CGGTGGTCGCCGTATCATTAGA2510580.1447011849464981No Hit
CGGTGGTCGCCGTATCATTAGAA2294850.13226725070480574No Hit
CGGTGGTCGCCGTATCATTAGAG2242540.12925228245661158No Hit
CGGTGGTCGCCGTATCATTAGGC2219190.1279064688723001No Hit
CGGTGGTCGCCGTATCATTAG1918690.11058668377227432No Hit
CGGTGGTCGCCGTATCATTAGTA1878210.10825355598242725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)