FastQCFastQC Report
Thu 10 Apr 2025
trimmed_POR-71-TP2_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed_POR-71-TP2_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236709796
Total Bases25.3 Gbp
Sequences flagged as poor quality0
Sequence length20-151
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGG6478360.27368364594425154No Hit
CGGTGGTCGCCGTATCATTAGGA4944410.20888066668774452No Hit
CGGTGGTCGCCGTATCATTAGGG3642160.15386604447920693No Hit
CGGTGGTCGCCGTATCATTAGGT3261490.13778432726966652No Hit
CGGTGGTCGCCGTATCATTAGGC2685690.1134591827369916No Hit
CGGTGGTCGCCGTATCATTAAG2609920.11025821677443379No Hit
CGGTGGTCGCCGTATCATTAGT2380060.10054759203966364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)