Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF07-EM03-Larvae_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF07-EM03-Larvae_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 128061872 Number of paired-end alignments with a unique best hit: 42132656 Mapping efficiency: 32.9% Sequence pairs with no alignments under any condition: 70148503 Sequence pairs did not map uniquely: 15780713 Sequence pairs which were discarded because genomic sequence could not be extracted: 44 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 10067430 ((converted) top strand) GA/CT/CT: 10973939 (complementary to (converted) top strand) GA/CT/GA: 11003713 (complementary to (converted) bottom strand) CT/GA/GA: 10087530 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1472676037 Total methylated C's in CpG context: 38937577 Total methylated C's in CHG context: 4763094 Total methylated C's in CHH context: 20707519 Total methylated C's in Unknown context: 766513 Total unmethylated C's in CpG context: 173273221 Total unmethylated C's in CHG context: 302035165 Total unmethylated C's in CHH context: 932959461 Total unmethylated C's in Unknown context: 5448077 C methylated in CpG context: 18.3% C methylated in CHG context: 1.6% C methylated in CHH context: 2.2% C methylated in Unknown context (CN or CHN): 12.3% Bismark completed in 0d 11h 11m 11s