Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF07-EM01-Zygote_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF07-EM01-Zygote_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 236542126 Number of paired-end alignments with a unique best hit: 23579759 Mapping efficiency: 10.0% Sequence pairs with no alignments under any condition: 202949461 Sequence pairs did not map uniquely: 10012906 Sequence pairs which were discarded because genomic sequence could not be extracted: 53 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6970090 ((converted) top strand) GA/CT/CT: 4852195 (complementary to (converted) top strand) GA/CT/GA: 4827905 (complementary to (converted) bottom strand) CT/GA/GA: 6929516 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 538311693 Total methylated C's in CpG context: 10902218 Total methylated C's in CHG context: 1510834 Total methylated C's in CHH context: 11438727 Total methylated C's in Unknown context: 424447 Total unmethylated C's in CpG context: 57349452 Total unmethylated C's in CHG context: 98086035 Total unmethylated C's in CHH context: 359024427 Total unmethylated C's in Unknown context: 2507832 C methylated in CpG context: 16.0% C methylated in CHG context: 1.5% C methylated in CHH context: 3.1% C methylated in Unknown context (CN or CHN): 14.5% Bismark completed in 0d 10h 41m 11s