Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF05-EM06-Larvae_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF05-EM06-Larvae_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 197315016 Number of paired-end alignments with a unique best hit: 69096435 Mapping efficiency: 35.0% Sequence pairs with no alignments under any condition: 106977420 Sequence pairs did not map uniquely: 21241161 Sequence pairs which were discarded because genomic sequence could not be extracted: 35 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 16463519 ((converted) top strand) GA/CT/CT: 18062442 (complementary to (converted) top strand) GA/CT/GA: 18070671 (complementary to (converted) bottom strand) CT/GA/GA: 16499768 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2770765034 Total methylated C's in CpG context: 75491086 Total methylated C's in CHG context: 24300011 Total methylated C's in CHH context: 100612703 Total methylated C's in Unknown context: 1879039 Total unmethylated C's in CpG context: 306626655 Total unmethylated C's in CHG context: 515819901 Total unmethylated C's in CHH context: 1747914678 Total unmethylated C's in Unknown context: 10676837 C methylated in CpG context: 19.8% C methylated in CHG context: 4.5% C methylated in CHH context: 5.4% C methylated in Unknown context (CN or CHN): 15.0% Bismark completed in 0d 13h 27m 13s