Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF04-EM04-Zygote_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF04-EM04-Zygote_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 156051705 Number of paired-end alignments with a unique best hit: 13101846 Mapping efficiency: 8.4% Sequence pairs with no alignments under any condition: 137152854 Sequence pairs did not map uniquely: 5797005 Sequence pairs which were discarded because genomic sequence could not be extracted: 39 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3227099 ((converted) top strand) GA/CT/CT: 3293198 (complementary to (converted) top strand) GA/CT/GA: 3327067 (complementary to (converted) bottom strand) CT/GA/GA: 3254443 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 277701930 Total methylated C's in CpG context: 6445114 Total methylated C's in CHG context: 2039614 Total methylated C's in CHH context: 9515860 Total methylated C's in Unknown context: 462087 Total unmethylated C's in CpG context: 30876926 Total unmethylated C's in CHG context: 50171833 Total unmethylated C's in CHH context: 178652583 Total unmethylated C's in Unknown context: 2103819 C methylated in CpG context: 17.3% C methylated in CHG context: 3.9% C methylated in CHH context: 5.1% C methylated in Unknown context (CN or CHN): 18.0% Bismark completed in 0d 6h 59m 32s