Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF03-EM03-Zygote_R1_001.fastp-trim.REPAIRED.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF03-EM03-Zygote_R2_001.fastp-trim.REPAIRED.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 133337327 Number of paired-end alignments with a unique best hit: 21524633 Mapping efficiency: 16.1% Sequence pairs with no alignments under any condition: 103555667 Sequence pairs did not map uniquely: 8257027 Sequence pairs which were discarded because genomic sequence could not be extracted: 21 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 6049554 ((converted) top strand) GA/CT/CT: 4687480 (complementary to (converted) top strand) GA/CT/GA: 4703312 (complementary to (converted) bottom strand) CT/GA/GA: 6084266 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 492559811 Total methylated C's in CpG context: 12916237 Total methylated C's in CHG context: 3461459 Total methylated C's in CHH context: 19406022 Total methylated C's in Unknown context: 595330 Total unmethylated C's in CpG context: 47347964 Total unmethylated C's in CHG context: 87279346 Total unmethylated C's in CHH context: 322148783 Total unmethylated C's in Unknown context: 2693339 C methylated in CpG context: 21.4% C methylated in CHG context: 3.8% C methylated in CHH context: 5.7% C methylated in Unknown context (CN or CHN): 18.1% Bismark completed in 0d 6h 41m 33s