Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF02-EM02-Zygote_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/EF02-EM02-Zygote_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 142822546 Number of paired-end alignments with a unique best hit: 32256397 Mapping efficiency: 22.6% Sequence pairs with no alignments under any condition: 95841532 Sequence pairs did not map uniquely: 14724617 Sequence pairs which were discarded because genomic sequence could not be extracted: 49 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9153439 ((converted) top strand) GA/CT/CT: 7050972 (complementary to (converted) top strand) GA/CT/GA: 6977351 (complementary to (converted) bottom strand) CT/GA/GA: 9074586 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 896414654 Total methylated C's in CpG context: 20476573 Total methylated C's in CHG context: 3063299 Total methylated C's in CHH context: 15176232 Total methylated C's in Unknown context: 778818 Total unmethylated C's in CpG context: 110840827 Total unmethylated C's in CHG context: 183025506 Total unmethylated C's in CHH context: 563832217 Total unmethylated C's in Unknown context: 4778053 C methylated in CpG context: 15.6% C methylated in CHG context: 1.6% C methylated in CHH context: 2.6% C methylated in Unknown context (CN or CHN): 14.0% Bismark completed in 0d 6h 41m 35s